FastQCFastQC Report
Fri 27 May 2016
SRR2139449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5661958
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85000.049.964241
GTATCAA154900.037.3576161
TCAACGC182500.030.8246924
ATCAACG185050.030.6284963
TATCAAC186950.030.6137712
CAACGCA186850.030.0617985
AACGCAG192550.029.2597546
TGGTATC39800.026.6768882
ACGCAGA218500.025.7460257
CGCAGAG222900.025.2378038
GTGGTAT44250.025.0456621
GCAGAGT257350.021.7278759
GAGTACT141500.020.99906212-13
GTACATG197550.020.9200171
TACATGG198600.020.0213382
ACATGGG196500.019.6740443
CAGAGTA252650.019.42834510-11
GTACTTT155650.018.98186114-15
AGAGTAC235200.018.08234410-11
CATGGGG140600.017.538534
Produced by FastQC (version 0.11.5)