##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5661958
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.74290978491893	30.0	18.0	33.0	18.0	34.0
2	30.825131341489993	32.0	30.0	33.0	27.0	34.0
3	32.14123753655537	33.0	31.0	33.0	29.0	34.0
4	32.48428511832832	33.0	33.0	33.0	31.0	34.0
5	32.93171832076465	33.0	33.0	34.0	32.0	34.0
6	36.249063663135615	38.0	36.0	38.0	33.0	38.0
7	37.083750356325496	38.0	38.0	38.0	36.0	38.0
8	36.62604473576102	38.0	37.0	38.0	34.0	38.0
9	37.177390047753796	38.0	38.0	38.0	36.0	38.0
10-11	37.32610803541813	38.0	38.0	38.0	36.5	38.0
12-13	37.40139020812235	38.0	38.0	38.0	37.0	38.0
14-15	37.389503595752565	38.0	38.0	38.0	36.5	38.0
16-17	37.46828729566698	38.0	38.0	38.0	37.0	38.0
18-19	37.494905207703766	38.0	38.0	38.0	37.0	38.0
20-21	37.527867619734735	38.0	38.0	38.0	37.0	38.0
22-23	37.53934172385341	38.0	38.0	38.0	37.5	38.0
24-25	37.55958392466718	38.0	38.0	38.0	38.0	38.0
26-27	37.53221669719086	38.0	38.0	38.0	37.5	38.0
28-29	37.513526265324366	38.0	38.0	38.0	37.0	38.0
30-31	37.512078864340765	38.0	38.0	38.0	37.5	38.0
32-33	37.430166041349004	38.0	38.0	38.0	37.0	38.0
34-35	37.466814400637574	38.0	38.0	38.0	37.0	38.0
36-37	37.45098811579854	38.0	38.0	38.0	37.0	38.0
38-39	37.43467649069468	38.0	38.0	38.0	37.0	38.0
40-41	37.43594853340721	38.0	38.0	38.0	37.0	38.0
42-43	37.40185748031606	38.0	38.0	38.0	37.0	38.0
44-45	37.360601985393686	38.0	38.0	38.0	37.0	38.0
46-47	37.33015942958608	38.0	38.0	38.0	37.0	38.0
48-49	37.21015812309089	38.0	38.0	38.0	37.0	38.0
50-51	37.19162737254292	38.0	38.0	38.0	37.0	38.0
52-53	37.192505653221296	38.0	38.0	38.0	37.0	38.0
54-55	37.16971669208845	38.0	38.0	38.0	37.0	38.0
56-57	37.11230168018997	38.0	38.0	38.0	36.0	38.0
58-59	37.090098359413346	38.0	38.0	38.0	36.0	38.0
60-61	37.10174508342898	38.0	38.0	38.0	36.0	38.0
62-63	37.10299267688745	38.0	38.0	38.0	36.0	38.0
64-65	37.107458934627324	38.0	38.0	38.0	36.0	38.0
66-67	37.046794445243705	38.0	38.0	38.0	36.0	38.0
68-69	37.061755226253624	38.0	38.0	38.0	36.0	38.0
70-71	37.078281775957635	38.0	38.0	38.0	36.0	38.0
72-73	37.090517941227105	38.0	38.0	38.0	36.0	38.0
74-75	37.07675370063343	38.0	38.0	38.0	36.0	38.0
76-77	37.042494176798	38.0	38.0	38.0	36.0	38.0
78-79	37.04207817073983	38.0	38.0	38.0	36.0	38.0
80-81	37.03957631913063	38.0	38.0	38.0	36.0	38.0
82-83	36.97607198825952	38.0	38.0	38.0	36.0	38.0
84-85	36.97457818510231	38.0	38.0	38.0	36.0	38.0
86-87	36.96865551732087	38.0	38.0	38.0	36.0	38.0
88-89	36.946349417091525	38.0	38.0	38.0	35.0	38.0
90-91	36.92068388182654	38.0	38.0	38.0	35.0	38.0
92-93	36.90782533474235	38.0	38.0	38.0	35.0	38.0
94-95	36.900156083391764	38.0	38.0	38.0	35.0	38.0
96-97	36.86932343296084	38.0	38.0	38.0	35.0	38.0
98-99	36.89595616979396	38.0	38.0	38.0	35.0	38.0
100	35.47837615778051	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	10.0
20	18.0
21	72.0
22	276.0
23	819.0
24	1910.0
25	3957.0
26	7325.0
27	12461.0
28	20380.0
29	30181.0
30	40578.0
31	51893.0
32	66377.0
33	89848.0
34	138351.0
35	268706.0
36	903934.0
37	4024860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09134472562318	18.764409767787043	13.500947905300603	22.643297601289163
2	14.688518602123303	20.111001247359823	43.26453954900754	21.935940601509337
3	18.730555530534758	26.049403059754784	29.934049304860494	25.285992104849964
4	12.759543606646323	18.08427402675894	38.59126118561812	30.56492118097662
5	13.881205053092941	37.26306341375192	35.21582109934408	13.639910433811059
6	30.242612184689467	37.34494321575681	18.729651473924743	13.682793125628978
7	27.193596278884442	32.52111725307747	22.532046334501246	17.753240133536842
8	24.46904410099828	36.86539179555906	20.83987553422332	17.82568856921934
9	25.64780593568515	17.723709713141638	21.40854806764727	35.219936283525946
10-11	24.636971874393982	26.960240609343977	28.24084000623106	20.16194751003098
12-13	25.47232777071112	24.281061427866472	28.683778650424465	21.56283215099794
14-15	22.563660839589414	25.62173721528842	26.554833151358594	25.25976879376357
16-17	21.93823232175159	28.886738474570105	27.241856968914284	21.93317223476402
18-19	21.929878321245052	27.812163212796705	29.199766229279696	21.05819223667855
20-21	22.762637134973108	26.86305793935228	29.18386239800124	21.19044252767338
22-23	22.519515560519963	26.82520564727227	29.05054298792787	21.604735804279894
24-25	22.23666267456608	27.008256229266692	29.125747635651933	21.629333460515298
26-27	22.157028494505408	27.127769047789403	29.096477118450192	21.618725339255004
28-29	22.059473508510038	27.091369472018727	29.148140876298235	21.701016143173
30-31	22.41989240196824	26.98847411638903	29.051353573897927	21.540279907744807
32-33	22.163055881693484	27.207442014853566	28.99155808364536	21.63794401980759
34-35	22.286475571042747	27.201838973520744	29.059812873383677	21.45187258205283
36-37	22.251916491898918	27.076845866677623	28.9891272655995	21.68211037582396
38-39	22.054289206874238	27.134581505716543	29.17594556698066	21.63518372042856
40-41	22.31308758103286	27.028718458653778	29.017739777963985	21.640454182349377
42-43	22.25778851697488	27.051778506248453	28.901636213710752	21.788796763065918
44-45	22.212976050975588	27.219133982631604	28.77891953890423	21.78897042748857
46-47	22.377630041386702	27.194060940115865	28.43245790512802	21.995851113369405
48-49	22.311863729653766	27.32189119322831	28.32998303044626	22.036262046671666
50-51	22.23757510002137	27.464551729933877	28.31879110851332	21.979082061531432
52-53	22.410181757398437	27.413371236905533	28.263129483327216	21.913317522368814
54-55	22.397253167245832	27.329787732240735	28.23877312598872	22.034185974524714
56-57	22.367742944911377	27.49757717842239	28.123616232046118	22.011063644620112
58-59	22.268161925708842	27.519461565793453	28.29171842224526	21.920658086252445
60-61	22.386329056463786	27.6320664176866	28.192039465971234	21.78956505987838
62-63	22.272229690906087	27.72947903414292	28.17118695016292	21.82710432478808
64-65	22.263496454456106	27.635991429855594	28.199476514685966	21.901035601002334
66-67	22.348945921377954	27.692291761376964	27.98927008083276	21.969492236412325
68-69	22.244809140992373	27.690955960159386	28.19687375795601	21.86736114089224
70-71	22.423455873299147	27.41937240646952	28.22216549155698	21.93500622867435
72-73	22.532269811510165	27.29627804775862	28.159365889851244	22.01208625087997
74-75	22.353357300710357	27.36130534846517	28.169807079493403	22.115530271331075
76-77	22.362282945321283	27.43232156979482	27.99029364422662	22.215101840657276
78-79	22.40285491566924	27.414647132600617	27.9534742949567	22.229023656773446
80-81	22.375442121672453	27.45610428786287	27.96048672653917	22.207966863925506
82-83	22.433558385030963	27.41038150164804	28.008158722796427	22.14790139052457
84-85	22.472251898589573	27.487384891708338	27.91823102719762	22.122132182504465
86-87	22.336638197719967	27.484894613385013	28.017323888929933	22.16114329996509
88-89	22.414010130244698	27.52747598559181	27.90954930170999	22.148964582453498
90-91	22.466175988766405	27.54744968160447	27.886148211542828	22.1002261180863
92-93	22.564431892284738	27.527096216503754	27.857523654871603	22.050948236339902
94-95	22.379961395402525	27.627320932052935	27.888965397259813	22.103752275284723
96-97	22.534768924549407	27.684379788029883	27.95877153878673	21.82207974863398
98-99	23.529890152850047	29.569985289984206	28.196159831111743	18.703964726054004
100	29.979671453852884	40.526320021461565	29.49400852468555	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	849.0
1	823.5
2	1127.0
3	1527.0
4	1961.0
5	2854.5
6	3677.5
7	4676.5
8	6167.0
9	7119.0
10	7151.0
11	6987.0
12	7508.0
13	9361.5
14	13082.5
15	17411.0
16	21005.0
17	23644.5
18	24070.0
19	22344.0
20	19990.0
21	18088.0
22	17682.5
23	19686.0
24	24237.5
25	31171.0
26	40703.5
27	51599.5
28	63043.0
29	76099.0
30	88715.5
31	100940.5
32	115643.0
33	131599.0
34	146077.0
35	160466.5
36	175959.5
37	188941.5
38	199680.5
39	208467.5
40	217020.0
41	224840.0
42	230498.5
43	236523.5
44	244247.0
45	252094.0
46	252307.0
47	249106.5
48	247583.5
49	243730.5
50	238459.0
51	231445.5
52	220418.0
53	206905.5
54	192265.5
55	176972.0
56	160823.5
57	144427.5
58	128177.5
59	110261.5
60	92225.5
61	74957.5
62	58973.5
63	45415.5
64	34273.5
65	25592.5
66	18916.0
67	14277.5
68	10890.5
69	7947.5
70	5733.0
71	4025.0
72	2662.0
73	1743.5
74	1140.5
75	764.5
76	529.0
77	339.0
78	202.5
79	130.0
80	85.5
81	49.5
82	36.0
83	31.5
84	25.0
85	15.5
86	8.5
87	5.5
88	3.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007064693874451205
3	1.7661734686128014E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.3277009336087396E-4
26-27	4.0631821287823806E-4
28-29	0.0030218826717825433
30-31	0.013771627846213816
32-33	0.008519298116215638
34-35	6.490494492993244E-4
36-37	1.0716001231268541E-4
38-39	8.269148561725419E-4
40-41	9.05293859744814E-6
42-43	0.0018147600545230522
44-45	6.713852480290244E-4
46-47	0.0014673944943358573
48-49	0.003497884576936018
50-51	0.0023186514117700613
52-53	0.0
54-55	0.0017404920572981523
56-57	0.05114141828580558
58-59	0.07048469525493041
60-61	0.048056984447918734
62-63	0.01409623976080026
64-65	0.0
66-67	0.0
68-69	2.4622804287076453E-4
70-71	0.001804929261814014
72-73	0.006094259417413974
74-75	0.010443764669993983
76-77	0.019021317890619293
78-79	0.013330764485286532
80-81	0.006842395262439196
82-83	0.039546554935954804
84-85	0.028266913343490224
86-87	0.008617209673065998
88-89	0.002838960156163331
90-91	0.0033212549592506234
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	31.0
20-21	184.0
22-23	458.0
24-25	504.0
26-27	1338.0
28-29	3669.0
30-31	12197.0
32-33	15281.0
34-35	21366.0
36-37	34334.0
38-39	39720.0
40-41	39471.0
42-43	44212.0
44-45	52090.0
46-47	52593.0
48-49	49764.0
50-51	44183.0
52-53	40226.0
54-55	42757.0
56-57	44135.0
58-59	46641.0
60-61	46611.0
62-63	44900.0
64-65	47580.0
66-67	51066.0
68-69	52454.0
70-71	55456.0
72-73	46856.0
74-75	46287.0
76-77	48376.0
78-79	50049.0
80-81	50218.0
82-83	46802.0
84-85	45479.0
86-87	47122.0
88-89	49317.0
90-91	50046.0
92-93	48882.0
94-95	56490.0
96-97	171424.0
98-99	1285335.0
100-101	2736054.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.36873041761329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.81643796753345	81.13702054658476
2	6.033299655777288	10.663100616201245
3	1.2560578426967	3.329887106705808
4	0.4084857480973079	1.4438946781219237
5	0.1841526175206458	0.8136666506689899
6	0.10234202336890541	0.5426300804087929
7	0.054544844901515926	0.3374041086340813
8	0.03682066666205252	0.2603036452844576
9	0.02181675023667508	0.1735126668227827
>10	0.08452526576209811	1.130803618476587
>50	0.0010755464674267708	0.06677976966865731
>100	3.8377514321855685E-4	0.06368102695029743
>500	5.729583276386071E-5	0.037315485471605285
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.532346937225603E-5	0.0	0.0	0.0
3	0.0	3.532346937225603E-5	0.0	0.0	0.0
4	0.0	7.064693874451206E-5	0.0	0.0	0.0
5	0.0	8.830867343064008E-5	0.0	0.0	0.0
6	3.532346937225603E-5	8.830867343064008E-5	0.0	0.0	3.532346937225603E-5
7	3.532346937225603E-5	1.0597040811676809E-4	0.0	0.0	3.532346937225603E-5
8	5.298520405838404E-5	1.0597040811676809E-4	0.0	1.7661734686128014E-5	3.532346937225603E-5
9	5.298520405838404E-5	1.7661734686128016E-4	0.0	1.7661734686128014E-5	5.298520405838404E-5
10-11	5.298520405838404E-5	1.8544821420434415E-4	0.0	4.415433671532004E-5	5.298520405838404E-5
12-13	5.298520405838404E-5	1.9427908154740815E-4	0.0	5.298520405838404E-5	5.298520405838404E-5
14-15	5.298520405838404E-5	3.002494896641762E-4	0.0	7.947780608757607E-5	5.298520405838404E-5
16-17	8.830867343064008E-5	3.002494896641762E-4	0.0	8.830867343064008E-5	5.298520405838404E-5
18-19	8.830867343064008E-5	3.4440382637949626E-4	0.0	8.830867343064008E-5	5.298520405838404E-5
20-21	8.830867343064008E-5	4.0621989778094435E-4	0.0	8.830867343064008E-5	7.064693874451206E-5
22-23	8.830867343064008E-5	4.3271249981013634E-4	0.0	8.830867343064008E-5	1.0597040811676809E-4
24-25	8.830867343064008E-5	5.210211732407764E-4	0.0	1.148012754598321E-4	1.5895561217515214E-4
26-27	8.830867343064008E-5	6.004989793283524E-4	0.0	2.2077168357660017E-4	1.5895561217515214E-4
28-29	8.830867343064008E-5	6.004989793283524E-4	0.0	4.4154336715320034E-4	1.7661734686128016E-4
30-31	8.830867343064008E-5	6.004989793283524E-4	0.0	0.0015718943870653933	2.1194081623353617E-4
32-33	8.830867343064008E-5	6.004989793283524E-4	0.0	0.004397771936845875	2.296025509196642E-4
34-35	8.830867343064008E-5	6.888076527589925E-4	0.0	0.008080243618903566	2.296025509196642E-4
36-37	8.830867343064008E-5	7.682854588465687E-4	0.0	0.014358990299822075	2.296025509196642E-4
38-39	8.830867343064008E-5	7.771163261896326E-4	0.0	0.03165865942488447	2.296025509196642E-4
40-41	8.830867343064008E-5	8.389323975910806E-4	0.0	0.050088679569859046	2.472642856057922E-4
42-43	8.830867343064008E-5	8.919176016494647E-4	0.0	0.060570919106076024	2.560951529488562E-4
44-45	8.830867343064008E-5	9.802262750801048E-4	0.0	0.07318139767197143	2.6492602029192023E-4
46-47	8.830867343064008E-5	0.0010420423464815528	0.0	0.08584486144192521	2.7375688763498423E-4
48-49	8.830867343064008E-5	0.0010420423464815528	0.0	0.09854364868125126	3.002494896641762E-4
50-51	1.0597040811676809E-4	0.0011480127545983208	0.0	0.11143671500212471	3.002494896641762E-4
52-53	1.0597040811676809E-4	0.001165674489284449	0.0	0.1543017450853574	3.090803570072403E-4
54-55	1.0597040811676809E-4	0.001165674489284449	0.0	0.20605062771571248	3.1791122435030427E-4
56-57	1.0597040811676809E-4	0.001183336223970577	0.0	0.2768830146744289	3.1791122435030427E-4
58-59	1.0597040811676809E-4	0.001209828825999769	0.0	0.3210020279203767	3.1791122435030427E-4
60-61	1.236321428028961E-4	0.001253983162715089	0.0	0.3359615171995271	3.1791122435030427E-4
62-63	1.236321428028961E-4	0.001253983162715089	0.0	0.34841304015324737	3.708964284086883E-4
64-65	1.236321428028961E-4	0.0013952770402041131	0.0	0.3554159179562971	3.708964284086883E-4
66-67	1.236321428028961E-4	0.0013952770402041131	0.0	0.3619772523921937	3.797272957517523E-4
68-69	1.236321428028961E-4	0.0014129387748902413	0.0	0.36889182152181277	3.885581630948163E-4
70-71	1.501247448320881E-4	0.0014394313769194333	0.0	0.3745965618254321	4.0621989778094435E-4
72-73	1.7661734686128016E-4	0.0014482622442624972	0.0	0.3790384880989933	4.945285712115844E-4
74-75	1.8544821420434415E-4	0.0014659239789486251	0.0	0.38249135723013133	5.121903058977124E-4
76-77	1.9427908154740815E-4	0.0014659239789486251	0.0	0.38611201284078756	5.298520405838405E-4
78-79	1.9427908154740815E-4	0.0014659239789486251	0.0	0.3885051778907579	5.475137752699685E-4
80-81	1.9427908154740815E-4	0.0014659239789486251	0.0	0.38937060289037817	5.475137752699685E-4
82-83	1.9427908154740815E-4	0.0014659239789486251	0.0	0.3900859031451664	5.828372446422245E-4
84-85	1.9427908154740815E-4	0.0014659239789486251	0.0	0.3903331674307722	5.828372446422245E-4
86-87	1.9427908154740815E-4	0.0015012474483208813	0.0	0.390447968706232	6.004989793283524E-4
88	1.9427908154740815E-4	0.0015542326523792652	0.0	0.390447968706232	6.181607140144805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8500	0.0	49.96424	1
GTATCAA	15490	0.0	37.357616	1
TCAACGC	18250	0.0	30.824692	4
ATCAACG	18505	0.0	30.628496	3
TATCAAC	18695	0.0	30.613771	2
CAACGCA	18685	0.0	30.061798	5
AACGCAG	19255	0.0	29.259754	6
TGGTATC	3980	0.0	26.676888	2
ACGCAGA	21850	0.0	25.746025	7
CGCAGAG	22290	0.0	25.237803	8
GTGGTAT	4425	0.0	25.045662	1
GCAGAGT	25735	0.0	21.727875	9
GAGTACT	14150	0.0	20.999062	12-13
GTACATG	19755	0.0	20.920017	1
TACATGG	19860	0.0	20.021338	2
ACATGGG	19650	0.0	19.674044	3
CAGAGTA	25265	0.0	19.428345	10-11
GTACTTT	15565	0.0	18.981861	14-15
AGAGTAC	23520	0.0	18.082344	10-11
CATGGGG	14060	0.0	17.53853	4
>>END_MODULE