FastQCFastQC Report
Fri 27 May 2016
SRR2139448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094541
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58000.045.9597741
GTATCAA102150.036.0563961
TCAACGC118400.030.4656014
ATCAACG120050.030.1519343
CAACGCA122700.029.4664675
TATCAAC123450.029.4265422
AACGCAG127100.028.4463836
ACGCAGA143600.025.119277
GTGGTAT31250.024.3525891
CGCAGAG148350.024.2866428
TGGTATC29000.024.2122462
GTACATG153500.020.899351
GCAGAGT176650.020.4196289
TACATGG154950.020.2424872
ACATGGG152650.019.719253
GAGTACT83400.018.9285812-13
CAGAGTA168300.018.41015810-11
CATGGGG103800.017.94244
AGAGTAC157400.017.4681610-11
GTACTTT90950.017.33426314-15
Produced by FastQC (version 0.11.5)