##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139448_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4094541
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.012031385202885	31.0	18.0	33.0	18.0	34.0
2	31.015381699682578	32.0	31.0	33.0	27.0	34.0
3	32.257611536921964	33.0	33.0	33.0	30.0	34.0
4	32.50783518836421	33.0	33.0	33.0	31.0	34.0
5	32.980891631076595	33.0	33.0	34.0	32.0	34.0
6	36.38204086856133	38.0	37.0	38.0	34.0	38.0
7	37.1886543571062	38.0	38.0	38.0	36.0	38.0
8	36.768913536340214	38.0	37.0	38.0	34.0	38.0
9	37.30654107505579	38.0	38.0	38.0	36.0	38.0
10-11	37.461572737945474	38.0	38.0	38.0	37.0	38.0
12-13	37.4929614821295	38.0	38.0	38.0	37.0	38.0
14-15	37.451287824447235	38.0	38.0	38.0	37.0	38.0
16-17	37.527221610432036	38.0	38.0	38.0	37.0	38.0
18-19	37.5510830151658	38.0	38.0	38.0	37.0	38.0
20-21	37.580360167229756	38.0	38.0	38.0	37.5	38.0
22-23	37.59063102970465	38.0	38.0	38.0	38.0	38.0
24-25	37.60779378437128	38.0	38.0	38.0	38.0	38.0
26-27	37.5856659562718	38.0	38.0	38.0	38.0	38.0
28-29	37.57592492295103	38.0	38.0	38.0	38.0	38.0
30-31	37.573691070101916	38.0	38.0	38.0	38.0	38.0
32-33	37.47389487585998	38.0	38.0	38.0	38.0	38.0
34-35	37.53662285649472	38.0	38.0	38.0	38.0	38.0
36-37	37.53121022072423	38.0	38.0	38.0	38.0	38.0
38-39	37.52387721413169	38.0	38.0	38.0	38.0	38.0
40-41	37.51055056745464	38.0	38.0	38.0	38.0	38.0
42-43	37.478286690168616	38.0	38.0	38.0	38.0	38.0
44-45	37.44414161586558	38.0	38.0	38.0	37.0	38.0
46-47	37.42081662435443	38.0	38.0	38.0	37.0	38.0
48-49	37.288614422954396	38.0	38.0	38.0	37.0	38.0
50-51	37.28213580876827	38.0	38.0	38.0	37.0	38.0
52-53	37.29147337647571	38.0	38.0	38.0	37.0	38.0
54-55	37.27011986203222	38.0	38.0	38.0	37.0	38.0
56-57	37.20991693091413	38.0	38.0	38.0	37.0	38.0
58-59	37.187336458538276	38.0	38.0	38.0	36.5	38.0
60-61	37.19454396552403	38.0	38.0	38.0	36.0	38.0
62-63	37.19346574565168	38.0	38.0	38.0	36.0	38.0
64-65	37.19635066485134	38.0	38.0	38.0	36.0	38.0
66-67	37.102345452566865	38.0	38.0	38.0	36.0	38.0
68-69	37.125522843300644	38.0	38.0	38.0	36.0	38.0
70-71	37.147100193114426	38.0	38.0	38.0	36.0	38.0
72-73	37.16055948026546	38.0	38.0	38.0	36.0	38.0
74-75	37.1464066731771	38.0	38.0	38.0	36.0	38.0
76-77	37.111032678420116	38.0	38.0	38.0	36.0	38.0
78-79	37.10984011545132	38.0	38.0	38.0	36.0	38.0
80-81	37.10326808432029	38.0	38.0	38.0	36.0	38.0
82-83	37.039831383753594	38.0	38.0	38.0	36.0	38.0
84-85	37.033155598411675	38.0	38.0	38.0	36.0	38.0
86-87	37.026688734919794	38.0	38.0	38.0	36.0	38.0
88-89	37.005507681048684	38.0	38.0	38.0	36.0	38.0
90-91	36.97735189408185	38.0	38.0	38.0	35.5	38.0
92-93	36.95671592172711	38.0	38.0	38.0	35.0	38.0
94-95	36.9481745088503	38.0	38.0	38.0	35.0	38.0
96-97	36.917397581300875	38.0	38.0	38.0	35.0	38.0
98-99	36.93763831450084	38.0	38.0	38.0	35.0	38.0
100	35.467652414981536	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	14.0
21	45.0
22	165.0
23	499.0
24	1112.0
25	2372.0
26	4184.0
27	7090.0
28	11684.0
29	17558.0
30	23713.0
31	30154.0
32	38903.0
33	54526.0
34	87033.0
35	176312.0
36	646341.0
37	2992831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.256086091212666	18.845604427944426	13.729988294170214	22.168321186672692
2	15.012544281116948	20.372147006469486	43.08320649020003	21.53210222221354
3	19.32212165469137	26.177983363210522	29.686802424692395	24.813092557405717
4	13.223484634785681	18.086544987582247	38.59416720946255	30.09580316816952
5	14.405033433539927	37.54044714657882	34.89770892512738	13.156810494753868
6	30.981885393258974	37.236383760719455	18.67212955005213	13.109601295969439
7	28.14244624733273	32.59713359812492	22.23675376556249	17.023666388979862
8	25.064054798816276	37.02813086985818	20.632715608416184	17.27509872290936
9	26.364420334293882	18.113312334642636	21.318140421600372	34.20412690946311
10-11	25.248580976475754	27.112391840746007	28.036036273662905	19.60299090911533
12-13	26.019570936034096	24.483037292824765	28.455057599862844	21.042334171278295
14-15	23.110429227598406	25.858893585386006	26.33939921471051	24.691277972305077
16-17	22.517603316220306	29.05301229124339	26.98800671430571	21.4413776782306
18-19	22.46436169524252	28.141615873427572	28.737079443092643	20.656942988237265
20-21	23.419225081481354	27.23889026607677	28.620709025166718	20.72117562727516
22-23	23.238346388680164	27.04956000409838	28.46026530301459	21.25182830420686
24-25	22.825923672378583	27.229340708756833	28.573932261623657	21.370803357240927
26-27	22.756786639719532	27.34428954375127	28.54442722722439	21.35449658930481
28-29	22.726325753757315	27.41297710511296	28.553365378917096	21.307331762212627
30-31	22.974210963927593	27.27569872753342	28.468725599415663	21.28136470912332
32-33	22.746071670617635	27.516450385011098	28.396370665989263	21.341107278382
34-35	22.960768058048018	27.45060377738062	28.409606529614862	21.1790216349565
36-37	22.946748784197382	27.32014224964744	28.401776687621	21.33133227853418
38-39	22.736616453018566	27.52232643797587	28.468789762008907	21.27226734699666
40-41	23.021464871835498	27.412360088003418	28.328105539923428	21.238069500237657
42-43	22.95185618491017	27.48393199650273	28.130858866479524	21.43335295210758
44-45	22.962211104116616	27.578872301817558	28.071882351711352	21.387034242354474
46-47	23.090442885760705	27.581259845317934	27.79559388142381	21.532703387497552
48-49	22.984785856476574	27.67680379462922	27.751788809328463	21.586621539565744
50-51	22.99917122114322	27.757120664976554	27.739384561135143	21.504323552745085
52-53	23.20694864868336	27.695824633024724	27.60803045932201	21.489196258969905
54-55	23.17961628409619	27.61892632491243	27.618886274962133	21.582571116029246
56-57	23.115181365634093	27.774487126771106	27.58462217913154	21.52570932846326
58-59	23.19852212672649	27.70116888518229	27.60756334909122	21.492745639000002
60-61	23.29741421653994	27.699089498546158	27.595109130611185	21.408387154302716
62-63	23.29015243424399	27.846390956064504	27.515051344469395	21.34840526522211
64-65	23.416059844650768	27.825215667588182	27.428467072077652	21.330257415683402
66-67	23.8372283713563	27.904963975115944	27.022607374851624	21.235200278676132
68-69	23.252528039096433	27.895443633429508	27.491529130428876	21.360499197045183
70-71	23.42029156507032	27.734888370176964	27.47460217827197	21.37021788648075
72-73	23.566496815379768	27.639754413903383	27.363707686154598	21.43004108456225
74-75	23.170197284709943	27.60230077061361	27.549639686149984	21.677862258526464
76-77	22.875815430099202	27.57394558437388	27.677920583731048	21.872318401795876
78-79	22.92205634008643	27.530248860519475	27.584800512053327	21.962894287340763
80-81	22.879580927316546	27.58874235312424	27.622711450740827	21.90896526881839
82-83	22.81421578570928	27.537354541072784	27.735998754383385	21.912430918834556
84-85	22.874682849070748	27.54872628986477	27.671311703141864	21.90527915792261
86-87	22.72327183169541	27.55000823696821	27.761931621419418	21.964788309916962
88-89	22.766781602861037	27.553324347734016	27.70592636639298	21.973967683011963
90-91	22.773171895017345	27.544631302385923	27.739972098822907	21.94222470377382
92-93	22.82805241416393	27.515357838249738	27.734726074223985	21.921863673362346
94-95	22.64394267714251	27.604847135202565	27.779971897807233	21.971238289847694
96-97	22.792217489049317	27.72722773304451	27.767247857060973	21.713306920845195
98-99	23.886564048125862	29.630192657298515	27.928314744627215	18.554928549948414
100	30.38834292898356	40.46985978562149	29.141797285394954	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	653.0
1	612.0
2	589.0
3	644.0
4	868.0
5	1309.5
6	1645.5
7	1976.0
8	2448.5
9	2802.5
10	2971.5
11	3119.5
12	3614.0
13	4745.0
14	6763.5
15	9211.0
16	11461.5
17	13207.5
18	13765.5
19	13218.5
20	12243.5
21	11345.0
22	11353.0
23	12986.5
24	16313.0
25	21186.0
26	27886.5
27	35784.5
28	44232.5
29	53561.0
30	62992.5
31	72794.5
32	83979.5
33	95632.0
34	106596.0
35	117693.5
36	130466.0
37	141200.0
38	149054.0
39	156381.5
40	163693.5
41	169603.0
42	173206.0
43	176901.5
44	181272.5
45	184514.0
46	184890.0
47	184339.0
48	183250.0
49	180889.0
50	177480.0
51	171721.0
52	163199.0
53	152615.0
54	141019.0
55	129865.5
56	117425.0
57	104749.0
58	92923.0
59	79922.0
60	66903.5
61	54150.5
62	42657.0
63	32978.5
64	24639.5
65	18363.0
66	13748.0
67	10422.0
68	8042.0
69	6007.0
70	4315.5
71	3010.5
72	2025.0
73	1330.5
74	861.5
75	545.5
76	337.5
77	197.0
78	128.0
79	86.0
80	58.5
81	41.5
82	24.0
83	14.5
84	15.0
85	12.5
86	8.5
87	4.5
88	2.0
89	1.0
90	2.0
91	4.0
92	4.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005983576669521687
3	2.442276191641505E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.8319650793259198E-4
26-27	1.5879012672307137E-4
28-29	0.001967222409033681
30-31	0.01025002351511958
32-33	0.005957974945612025
34-35	5.656422966651206E-4
36-37	2.470640758210001E-5
38-39	6.593265787606029E-4
40-41	1.2534669328529046E-5
42-43	0.0014022236993996209
44-45	5.099242736957348E-4
46-47	0.0016094751475792142
48-49	0.002613420159559052
50-51	0.0016409893406583598
52-53	0.0
54-55	0.001695419151000838
56-57	0.04660892458527823
58-59	0.06575823735826787
60-61	0.04533345565080632
62-63	0.012420226727357587
64-65	0.0
66-67	0.0
68-69	3.144628467149424E-4
70-71	0.0016483604669371421
72-73	0.005074077139425404
74-75	0.01013804140429312
76-77	0.01884798880300922
78-79	0.013114276016333703
80-81	0.006064448548667199
82-83	0.036771864376930835
84-85	0.02636444222501463
86-87	0.008045870667151853
88-89	0.002490284676491134
90-91	0.0037113373869385015
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	21.0
20-21	148.0
22-23	337.0
24-25	420.0
26-27	1036.0
28-29	2679.0
30-31	8781.0
32-33	11458.0
34-35	16156.0
36-37	26821.0
38-39	30301.0
40-41	30160.0
42-43	34389.0
44-45	38878.0
46-47	38064.0
48-49	37294.0
50-51	33673.0
52-53	30353.0
54-55	33345.0
56-57	35141.0
58-59	35468.0
60-61	34277.0
62-63	33658.0
64-65	35705.0
66-67	38053.0
68-69	40088.0
70-71	39518.0
72-73	35870.0
74-75	37570.0
76-77	39137.0
78-79	40534.0
80-81	40247.0
82-83	37750.0
84-85	37103.0
86-87	38145.0
88-89	40145.0
90-91	40582.0
92-93	39499.0
94-95	43469.0
96-97	124632.0
98-99	902379.0
100-101	1931257.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.17839023353925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.05052079643683	83.91146175815139
2	5.206250757757561	9.389826249734416
3	1.0081631079904432	2.7274357851425983
4	0.3506686068886193	1.264909218986139
5	0.14565566107837116	0.6567496522224744
6	0.07793695304295915	0.4216937379072598
7	0.0474080910680683	0.2992629735594401
8	0.027642647438634264	0.1994215558247444
9	0.019250442466970864	0.1562376521659315
>10	0.06538917567294435	0.8738528078450125
>50	8.543530035337358E-4	0.055061320000384904
>100	2.5940715507629406E-4	0.04408728846019225
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4422761916415052E-5
2	0.0	0.0	0.0	0.0	2.4422761916415052E-5
3	0.0	0.0	0.0	0.0	2.4422761916415052E-5
4	0.0	4.8845523832830104E-5	0.0	0.0	2.4422761916415052E-5
5	2.4422761916415052E-5	7.326828574924515E-5	0.0	0.0	2.4422761916415052E-5
6	1.2211380958207526E-4	7.326828574924515E-5	0.0	0.0	2.4422761916415052E-5
7	1.2211380958207526E-4	7.326828574924515E-5	0.0	4.8845523832830104E-5	4.8845523832830104E-5
8	1.465365714984903E-4	7.326828574924515E-5	0.0	4.8845523832830104E-5	4.8845523832830104E-5
9	1.465365714984903E-4	1.9538209533132041E-4	0.0	7.326828574924515E-5	4.8845523832830104E-5
10-11	1.465365714984903E-4	1.9538209533132041E-4	0.0	7.326828574924515E-5	4.8845523832830104E-5
12-13	1.465365714984903E-4	1.9538209533132041E-4	0.0	7.326828574924515E-5	4.8845523832830104E-5
14-15	1.7095933341490535E-4	3.174959049133957E-4	0.0	1.2211380958207526E-4	7.326828574924515E-5
16-17	2.808617620387731E-4	3.419186668298107E-4	0.0	1.343251905402828E-4	7.326828574924515E-5
18-19	3.052845239551881E-4	3.5413004778801825E-4	0.0	1.587479524566978E-4	9.769104766566021E-5
20-21	3.174959049133957E-4	3.9076419066264083E-4	0.0	1.7095933341490535E-4	9.769104766566021E-5
22-23	3.174959049133957E-4	4.518210954536784E-4	0.0	1.7095933341490535E-4	9.769104766566021E-5
24-25	3.4191866682981074E-4	5.006666192865086E-4	0.0	1.7095933341490535E-4	9.769104766566021E-5
26-27	3.6634142874622576E-4	5.250893812029236E-4	0.0	2.930731429969806E-4	9.769104766566021E-5
28-29	3.6634142874622576E-4	5.373007621611311E-4	0.0	8.181625241999042E-4	9.769104766566021E-5
30-31	3.9076419066264083E-4	6.105690479103762E-4	0.0	0.0021247802867281097	9.769104766566021E-5
32-33	3.9076419066264083E-4	6.105690479103762E-4	0.0	0.005006666192865086	9.769104766566021E-5
34-35	4.029755716208484E-4	6.716259527014139E-4	0.0	0.008425852861163192	9.769104766566021E-5
36-37	4.151869525790559E-4	7.69317000367074E-4	0.0	0.01475134819751469	9.769104766566021E-5
38-39	4.151869525790559E-4	8.303739051581118E-4	0.0	0.02919741187107419	1.0990242862386773E-4
40-41	4.151869525790559E-4	8.914308099491493E-4	0.0	0.04433952425925153	1.2211380958207526E-4
42-43	4.151869525790559E-4	9.158535718655645E-4	0.0	0.05492679155001745	1.465365714984903E-4
44-45	4.273983335372634E-4	0.0010257560004894321	0.0	0.0665031806983982	1.7095933341490535E-4
46-47	4.396097144954709E-4	0.0010990242862386774	0.0	0.0783604316088177	1.7095933341490535E-4
48-49	4.396097144954709E-4	0.0010990242862386774	0.0	0.09077940604331475	1.7095933341490535E-4
50-51	4.396097144954709E-4	0.0012455608577371675	0.0	0.1036746243351819	1.7095933341490535E-4
52-53	4.396097144954709E-4	0.0012699836196535827	0.0	0.13382452392099628	1.7095933341490535E-4
54-55	4.396097144954709E-4	0.0012699836196535827	0.0	0.17008011398591444	1.7095933341490535E-4
56-57	4.396097144954709E-4	0.0012699836196535827	0.0	0.2127222562919751	1.7095933341490535E-4
58-59	4.396097144954709E-4	0.0012699836196535827	0.0	0.2480986269279023	1.8317071437311288E-4
60-61	4.7624385737009354E-4	0.0012944063815699977	0.0	0.2610549021245605	1.9538209533132041E-4
62-63	5.128780002447161E-4	0.0013798860482774505	0.0	0.27562307960770205	2.0759347628952795E-4
64-65	5.128780002447161E-4	0.001465365714984903	0.0	0.2836093227543698	2.1980485724773546E-4
66-67	5.128780002447161E-4	0.0015019998578595256	0.0	0.29054538713863165	2.1980485724773546E-4
68-69	5.128780002447161E-4	0.0015508453816923556	0.0	0.2980065409040965	2.32016238205943E-4
70-71	5.617235240775462E-4	0.0015874795245669783	0.0	0.3039412720497853	2.442276191641505E-4
72-73	6.594145717432064E-4	0.0015874795245669783	0.0	0.3089723610045668	2.930731429969806E-4
74-75	6.594145717432064E-4	0.0016119022864833933	0.0	0.3128555801492768	2.930731429969806E-4
76-77	6.594145717432064E-4	0.001648536429358016	0.0	0.31642130338907337	2.930731429969806E-4
78-79	6.594145717432064E-4	0.0016851705722326386	0.0	0.3184728153900523	2.930731429969806E-4
80-81	6.594145717432064E-4	0.0016973819531908461	0.0	0.3189734820093388	3.052845239551881E-4
82-83	6.594145717432064E-4	0.0017095933341490536	0.0	0.3195229941524581	3.419186668298107E-4
84-85	6.594145717432064E-4	0.0017095933341490536	0.0	0.3196573193429984	3.419186668298107E-4
86-87	6.594145717432064E-4	0.0017340160960654686	0.0	0.31975501039066406	3.419186668298107E-4
88	6.594145717432064E-4	0.001856129905647544	0.0	0.3197916445335387	3.419186668298107E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5800	0.0	45.959774	1
GTATCAA	10215	0.0	36.056396	1
TCAACGC	11840	0.0	30.465601	4
ATCAACG	12005	0.0	30.151934	3
CAACGCA	12270	0.0	29.466467	5
TATCAAC	12345	0.0	29.426542	2
AACGCAG	12710	0.0	28.446383	6
ACGCAGA	14360	0.0	25.11927	7
GTGGTAT	3125	0.0	24.352589	1
CGCAGAG	14835	0.0	24.286642	8
TGGTATC	2900	0.0	24.212246	2
GTACATG	15350	0.0	20.89935	1
GCAGAGT	17665	0.0	20.419628	9
TACATGG	15495	0.0	20.242487	2
ACATGGG	15265	0.0	19.71925	3
GAGTACT	8340	0.0	18.92858	12-13
CAGAGTA	16830	0.0	18.410158	10-11
CATGGGG	10380	0.0	17.9424	4
AGAGTAC	15740	0.0	17.46816	10-11
GTACTTT	9095	0.0	17.334263	14-15
>>END_MODULE