FastQCFastQC Report
Fri 27 May 2016
SRR2139447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7810739
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106500.050.8284721
GTATCAA203050.040.083351
TCAACGC243900.032.3598024
ATCAACG247250.032.024223
TATCAAC249350.032.0115552
CAACGCA251650.031.43065
AACGCAG259100.030.5759456
ACGCAGA297050.026.6554077
CGCAGAG306700.025.789088
TGGTATC48850.023.776712
GTGGTAT54850.022.9533251
GCAGAGT353550.022.4076589
GTACATG294000.021.0797671
TACATGG294250.020.5000152
ACATGGG296700.019.8294143
GAGTACT180500.019.81997912-13
CAGAGTA346400.019.08906610-11
AGAGTAC319200.018.73044810-11
GTACTTT195650.018.1878614-15
CATGGGG216250.017.3862324
Produced by FastQC (version 0.11.5)