##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7810739
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.683820698656042	30.0	18.0	33.0	18.0	34.0
2	30.79457846434249	32.0	30.0	33.0	27.0	34.0
3	32.149834861976565	33.0	31.0	33.0	30.0	34.0
4	32.46267094573253	33.0	33.0	33.0	31.0	34.0
5	32.9421126477277	33.0	33.0	34.0	32.0	34.0
6	36.35867169546953	38.0	36.0	38.0	34.0	38.0
7	37.146360286779526	38.0	38.0	38.0	36.0	38.0
8	36.756499096948446	38.0	37.0	38.0	34.0	38.0
9	37.26365789459871	38.0	38.0	38.0	36.0	38.0
10-11	37.384581151668236	38.0	38.0	38.0	37.0	38.0
12-13	37.462027984803996	38.0	38.0	38.0	37.0	38.0
14-15	37.43175344868136	38.0	38.0	38.0	37.0	38.0
16-17	37.49921953607719	38.0	38.0	38.0	37.0	38.0
18-19	37.526931894664514	38.0	38.0	38.0	37.0	38.0
20-21	37.55627383847437	38.0	38.0	38.0	37.0	38.0
22-23	37.567659065325884	38.0	38.0	38.0	37.5	38.0
24-25	37.58317834056753	38.0	38.0	38.0	38.0	38.0
26-27	37.56063004032131	38.0	38.0	38.0	38.0	38.0
28-29	37.54715547940868	38.0	38.0	38.0	38.0	38.0
30-31	37.54426960634396	38.0	38.0	38.0	38.0	38.0
32-33	37.46105464227334	38.0	38.0	38.0	37.0	38.0
34-35	37.51017132559139	38.0	38.0	38.0	37.5	38.0
36-37	37.499116576943905	38.0	38.0	38.0	37.5	38.0
38-39	37.49102482442379	38.0	38.0	38.0	38.0	38.0
40-41	37.47665350582624	38.0	38.0	38.0	38.0	38.0
42-43	37.442745996459884	38.0	38.0	38.0	37.0	38.0
44-45	37.40601346946987	38.0	38.0	38.0	37.0	38.0
46-47	37.379282928769484	38.0	38.0	38.0	37.0	38.0
48-49	37.262973937870726	38.0	38.0	38.0	37.0	38.0
50-51	37.245085224940546	38.0	38.0	38.0	37.0	38.0
52-53	37.249003675799855	38.0	38.0	38.0	37.0	38.0
54-55	37.22696853055764	38.0	38.0	38.0	37.0	38.0
56-57	37.16879195922492	38.0	38.0	38.0	36.5	38.0
58-59	37.1469797521065	38.0	38.0	38.0	36.0	38.0
60-61	37.158690222578485	38.0	38.0	38.0	36.0	38.0
62-63	37.1600312954108	38.0	38.0	38.0	36.0	38.0
64-65	37.16226102336839	38.0	38.0	38.0	36.0	38.0
66-67	37.09800578996655	38.0	38.0	38.0	36.0	38.0
68-69	37.11255348062698	38.0	38.0	38.0	36.0	38.0
70-71	37.12459075009537	38.0	38.0	38.0	36.0	38.0
72-73	37.13590946875956	38.0	38.0	38.0	36.0	38.0
74-75	37.12184647248887	38.0	38.0	38.0	36.0	38.0
76-77	37.087672637321816	38.0	38.0	38.0	36.0	38.0
78-79	37.08675508085706	38.0	38.0	38.0	36.0	38.0
80-81	37.08189685707575	38.0	38.0	38.0	36.0	38.0
82-83	37.01820720814127	38.0	38.0	38.0	36.0	38.0
84-85	37.01451558907391	38.0	38.0	38.0	36.0	38.0
86-87	37.005768413398584	38.0	38.0	38.0	36.0	38.0
88-89	36.98211090926173	38.0	38.0	38.0	36.0	38.0
90-91	36.954018644312896	38.0	38.0	38.0	35.0	38.0
92-93	36.938089154437385	38.0	38.0	38.0	35.0	38.0
94-95	36.93036585793755	38.0	38.0	38.0	35.0	38.0
96-97	36.90250283419505	38.0	38.0	38.0	35.0	38.0
98-99	36.926801294471275	38.0	38.0	38.0	35.0	38.0
100	35.48818124730419	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	18.0
21	94.0
22	339.0
23	972.0
24	2285.0
25	4656.0
26	8579.0
27	14789.0
28	24338.0
29	36836.0
30	49469.0
31	64240.0
32	83587.0
33	113259.0
34	178697.0
35	352233.0
36	1228813.0
37	5647530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70888874919518	18.261741942727827	13.526082487201274	22.50328682087572
2	15.06577192757156	20.01875228785978	42.6600464748426	22.255429309726054
3	19.055743395277553	25.80406944952826	29.490162067932896	25.65002508726129
4	13.151393229245018	17.750919599285037	38.058242632355274	31.039444539114676
5	14.33857923046718	37.14234978277984	34.70890526491795	13.810165721835027
6	31.10909223826324	36.70488797538876	18.521166819170375	13.664852967177627
7	28.138477037832143	32.25436159113754	22.02513744218057	17.582023928849754
8	25.230980064754437	36.38674906433309	20.41592991393004	17.96634095698243
9	26.25476283358079	17.712190869519517	20.980370743408532	35.05267555349116
10-11	25.22064173441207	26.672962699176097	27.791614852320635	20.314780714091203
12-13	26.068794514834	24.00139730696417	28.197703187880172	21.73210499032166
14-15	23.152469951946927	25.25753325005483	26.10111540022013	25.488881397778112
16-17	22.53914770420571	28.527083032732243	26.721511242406127	22.21225802065592
18-19	22.566014560209986	27.60592179562011	28.526244443707565	21.301819200462337
20-21	23.513141014971847	26.60936463755944	28.480243666988446	21.39725068048027
22-23	23.31992419799018	26.41036947526147	28.348855691046758	21.920850635701587
24-25	22.942642353754334	26.6045347971718	28.41506432889911	22.037758520174748
26-27	22.765036484437314	26.750686658348506	28.450811558872118	22.033465298342065
28-29	22.75525622179735	26.762494035209077	28.441743608466318	22.040506134527252
30-31	23.036696114680662	26.65856843075604	28.30870155534093	21.99603389922236
32-33	22.75711695426928	26.896438192869628	28.266115396287482	22.08032945657361
34-35	22.975781275367964	26.78538939167392	28.30233255145236	21.936496781505756
36-37	22.97571002103948	26.6901384579517	28.248938646015837	22.085212874992983
38-39	22.791577802501237	26.887762598043512	28.34021039935805	21.9804492000972
40-41	23.087707490678635	26.75768145823627	28.16073676347708	21.993874287608016
42-43	22.974804629515834	26.824878004413975	27.998341755023016	22.20197561104717
44-45	22.940968674185406	26.923255809775476	27.95801887981989	22.17775663621923
46-47	23.113011279235625	26.911110544030386	27.643367707379184	22.33251046935481
48-49	23.010635573455637	27.026836727232485	27.608563784537694	22.353963914774184
50-51	22.976587266104406	27.210559287639935	27.510351988104	22.302501458151664
52-53	23.25020348794742	27.078953599235277	27.385970260577587	22.284872652239716
54-55	23.185114727571683	26.9829632626162	27.431747749930402	22.40017425988172
56-57	23.096887631966776	27.143833728940475	27.461438466246022	22.297840172846726
58-59	23.145795722648103	27.106325072719194	27.42917970878943	22.31869949584327
60-61	23.1950607519408	27.11182328866245	27.454052278854036	22.239063680542714
62-63	23.203137368408676	27.161071301231487	27.436249906222628	22.199541424137216
64-65	23.253359145123717	27.06664786491288	27.409395422063103	22.2705975679003
66-67	23.472242210614883	27.13529593565662	27.14735973382342	22.245102119905077
68-69	23.159958482638295	27.155051025227817	27.459808328870018	22.225182163263877
70-71	23.32294665641364	26.934958167697904	27.430369281419807	22.311725894468644
72-73	23.439065153833553	26.902503160281704	27.327863052348018	22.330568633536718
74-75	23.083869855813024	26.996884472437788	27.45212481781175	22.467120853937438
76-77	23.054872315023598	27.03725079574148	27.43398945694916	22.473887432285768
78-79	23.04900032056916	26.968371933552465	27.352852968433922	22.629774777444453
80-81	23.05056351615995	26.994619212057184	27.35527238079925	22.599544890983616
82-83	23.07451306492113	26.973986365865414	27.440739807618396	22.510760761595055
84-85	23.096233786298605	26.95377602324079	27.39055909403632	22.559431096424284
86-87	22.923818328257468	27.034266830422453	27.47665042471627	22.5652644166038
88-89	23.040659332203823	27.020479381871148	27.3750086154099	22.56385267051513
90-91	23.048412116491377	27.087717258906448	27.355107741076996	22.508762883525183
92-93	23.061728725887512	27.103520148119802	27.314579140213464	22.52017198577923
94-95	22.95237231950811	27.132901907530073	27.318241619346217	22.5964841536156
96-97	23.11856609164131	27.157335189320314	27.382620991570917	22.34147772746746
98-99	24.19021463396194	29.101053910210695	27.570555913870066	19.138175541957295
100	31.092242195891373	40.10016259440625	28.80759520970238	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	938.0
1	876.5
2	1100.5
3	1516.0
4	2065.5
5	3149.0
6	4113.5
7	5062.0
8	6419.0
9	7435.5
10	7775.5
11	8009.0
12	8853.5
13	11005.0
14	15388.5
15	20736.5
16	25290.5
17	28553.5
18	29164.5
19	27228.5
20	24384.5
21	21886.0
22	21294.5
23	23747.5
24	29161.0
25	37595.5
26	49482.5
27	63248.5
28	77103.5
29	92961.5
30	108609.0
31	123636.5
32	142528.0
33	163413.0
34	182718.5
35	201489.0
36	222537.0
37	239739.5
38	253942.5
39	267517.5
40	281876.0
41	294625.0
42	302135.5
43	311080.5
44	320718.0
45	328482.5
46	332117.0
47	334279.5
48	336636.0
49	338103.0
50	337001.0
51	331903.0
52	322184.5
53	307769.0
54	290380.0
55	272646.5
56	251407.0
57	227981.5
58	205551.0
59	179536.5
60	152015.0
61	124597.5
62	99330.0
63	77489.0
64	59158.0
65	44686.5
66	33316.0
67	25548.0
68	19708.0
69	14794.0
70	11007.0
71	7963.5
72	5574.0
73	3654.0
74	2365.0
75	1562.0
76	1010.5
77	617.0
78	378.0
79	240.5
80	155.5
81	107.5
82	77.5
83	45.5
84	33.5
85	31.0
86	24.5
87	19.0
88	11.5
89	5.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006299019849466228
3	2.5605771745797676E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.9450140272298557E-4
26-27	3.1373898888198664E-4
28-29	0.002747731536252859
30-31	0.012508721964450225
32-33	0.007613592878251646
34-35	5.927210250259703E-4
36-37	5.822015174112216E-5
38-39	6.639747049071832E-4
40-41	0.0
42-43	0.0016236333429343304
44-45	6.786899925763292E-4
46-47	0.0014773726941234144
48-49	0.003029136635069677
50-51	0.0019551018382469054
52-53	0.0
54-55	0.001715619114497433
56-57	0.04854540999295706
58-59	0.06712818975504677
60-61	0.04643688359101062
62-63	0.013376996040193413
64-65	0.0
66-67	0.0
68-69	2.1435709097322332E-4
70-71	0.0016052761769399613
72-73	0.005662699032372165
74-75	0.009651829288064935
76-77	0.01859414718662636
78-79	0.013265313651273697
80-81	0.005817281313597074
82-83	0.03813410445792918
84-85	0.02677964400787631
86-87	0.007887515286109899
88-89	0.0027093809162831582
90-91	0.003444098636335638
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	46.0
20-21	211.0
22-23	557.0
24-25	615.0
26-27	1855.0
28-29	5268.0
30-31	15419.0
32-33	20081.0
34-35	27163.0
36-37	45061.0
38-39	53429.0
40-41	51847.0
42-43	58042.0
44-45	68221.0
46-47	68017.0
48-49	65380.0
50-51	59815.0
52-53	56378.0
54-55	60207.0
56-57	62289.0
58-59	62490.0
60-61	61580.0
62-63	58660.0
64-65	61449.0
66-67	65754.0
68-69	67333.0
70-71	66858.0
72-73	62458.0
74-75	63169.0
76-77	66249.0
78-79	68499.0
80-81	68574.0
82-83	63999.0
84-85	63261.0
86-87	65219.0
88-89	68544.0
90-91	69149.0
92-93	67558.0
94-95	78118.0
96-97	240825.0
98-99	1817444.0
100-101	3783648.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.52111925646453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.10662996609328	78.82647596350941
2	6.268302092468623	10.846810257560477
3	1.432094683346732	3.7171930465317424
4	0.5270512004049117	1.824042390579846
5	0.23313173533247386	1.0085409337583748
6	0.14114930810080745	0.7327437671494451
7	0.08266487383427264	0.5006580185134988
8	0.05165445320167568	0.35753608844717183
9	0.03254423545811241	0.25341873094636247
>10	0.1212155330964978	1.6580799970219746
>50	0.002762758061038197	0.1615220558840879
>100	7.749516445652731E-4	0.0962430074879782
>500	2.420895703604468E-5	0.01673574260973408
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2802885872898838E-5	0.0	0.0	0.0
3	0.0	1.2802885872898838E-5	0.0	0.0	0.0
4	0.0	3.840865761869651E-5	0.0	0.0	0.0
5	0.0	3.840865761869651E-5	0.0	0.0	0.0
6	5.121154349159535E-5	3.840865761869651E-5	0.0	0.0	1.2802885872898838E-5
7	5.121154349159535E-5	3.840865761869651E-5	0.0	0.0	1.2802885872898838E-5
8	5.121154349159535E-5	3.840865761869651E-5	0.0	0.0	2.5605771745797676E-5
9	5.121154349159535E-5	7.681731523739303E-5	0.0	1.2802885872898838E-5	2.5605771745797676E-5
10-11	5.121154349159535E-5	7.681731523739303E-5	0.0	1.9204328809348257E-5	2.5605771745797676E-5
12-13	6.40144293644942E-5	7.681731523739303E-5	0.0	2.5605771745797676E-5	3.20072146822471E-5
14-15	6.40144293644942E-5	1.344303016654378E-4	0.0	2.5605771745797676E-5	5.761298642804477E-5
16-17	7.681731523739303E-5	1.4083174460188724E-4	0.0	2.5605771745797676E-5	6.40144293644942E-5
18-19	7.681731523739303E-5	1.728389592841343E-4	0.0	5.121154349159535E-5	6.40144293644942E-5
20-21	7.681731523739303E-5	2.048461739663814E-4	0.0	5.761298642804477E-5	7.04158723009436E-5
22-23	8.321875817384245E-5	2.4325483158507794E-4	0.0	8.321875817384245E-5	7.681731523739303E-5
24-25	1.280288587289884E-4	2.75262046267325E-4	0.0	1.024230869831907E-4	7.681731523739303E-5
26-27	1.4083174460188724E-4	2.8166348920377447E-4	0.0	1.280288587289884E-4	7.681731523739303E-5
28-29	1.5363463047478608E-4	2.8166348920377447E-4	0.0	3.7768513325051576E-4	7.681731523739303E-5
30-31	1.664375163476849E-4	3.648822473776169E-4	0.0	0.0012098727149889403	7.681731523739303E-5
32-33	1.664375163476849E-4	3.712836903140663E-4	0.0	0.0032583344546527546	7.681731523739303E-5
34-35	1.728389592841343E-4	4.545024484879088E-4	0.0	0.00540281783836331	1.024230869831907E-4
36-37	1.7924040222058374E-4	5.249183207888524E-4	0.0	0.008853195581109547	1.024230869831907E-4
38-39	1.7924040222058374E-4	5.377212066617512E-4	0.0	0.014012758587887779	1.0882452991964012E-4
40-41	1.920432880934826E-4	6.017356360262454E-4	0.0	0.02059984336949423	1.1522597285608955E-4
42-43	2.1764905983928025E-4	6.273414077720431E-4	0.0	0.026758031474358575	1.1522597285608955E-4
44-45	2.1764905983928025E-4	7.297644947552338E-4	0.0	0.032941825350968706	1.1522597285608955E-4
46-47	2.1764905983928025E-4	8.065818099926268E-4	0.0	0.03999621546693598	1.280288587289884E-4
48-49	2.1764905983928025E-4	8.193846958655256E-4	0.0	0.046973788267665836	1.280288587289884E-4
50-51	2.1764905983928025E-4	9.47413554594514E-4	0.0	0.0540537841553789	1.280288587289884E-4
52-53	2.1764905983928025E-4	9.602164404674128E-4	0.0	0.060711284809286295	1.280288587289884E-4
54-55	2.1764905983928025E-4	9.666178834038623E-4	0.0	0.06794491532747413	1.280288587289884E-4
56-57	2.1764905983928025E-4	9.986250980861095E-4	0.0	0.07526816604677228	1.280288587289884E-4
58-59	2.1764905983928025E-4	0.0010050265410225588	0.0	0.08236096482035823	1.280288587289884E-4
60-61	2.1764905983928025E-4	0.001056238084514154	0.0	0.08899926114545628	1.280288587289884E-4
62-63	2.1764905983928025E-4	0.0011010481850693	0.0	0.09517025213619351	1.280288587289884E-4
64-65	2.1764905983928025E-4	0.0012930914731627827	0.0	0.1013476445698672	1.280288587289884E-4
66-67	2.1764905983928025E-4	0.0013058943590356815	0.0	0.10726257784314647	1.280288587289884E-4
68-69	2.1764905983928025E-4	0.001344303016654378	0.0	0.11311989812999768	1.280288587289884E-4
70-71	2.1764905983928025E-4	0.0013507044595908275	0.0	0.11879797801462832	1.280288587289884E-4
72-73	2.1764905983928025E-4	0.0013763102313366251	0.0	0.12295891592332045	1.5363463047478605E-4
74-75	2.1764905983928025E-4	0.0014275217748282204	0.0	0.12614683450567227	1.5363463047478605E-4
76-77	2.1764905983928025E-4	0.0014787333183198157	0.0	0.12952679637611753	1.5363463047478605E-4
78-79	2.1764905983928025E-4	0.0015043390900656133	0.0	0.13176089996093837	1.5363463047478605E-4
80-81	2.1764905983928025E-4	0.0015107405330020628	0.0	0.132381839925774	1.5363463047478605E-4
82-83	2.1764905983928025E-4	0.0015299448618114114	0.0	0.13288755391775348	1.5363463047478605E-4
84-85	2.304519457121791E-4	0.0015363463047478607	0.0	0.13319482317870307	1.5363463047478605E-4
86-87	2.304519457121791E-4	0.0015491491906207595	0.0	0.13329724626568626	1.5363463047478605E-4
88	2.304519457121791E-4	0.001587557848239456	0.0	0.1333292534803685	1.5363463047478605E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10650	0.0	50.828472	1
GTATCAA	20305	0.0	40.08335	1
TCAACGC	24390	0.0	32.359802	4
ATCAACG	24725	0.0	32.02422	3
TATCAAC	24935	0.0	32.011555	2
CAACGCA	25165	0.0	31.4306	5
AACGCAG	25910	0.0	30.575945	6
ACGCAGA	29705	0.0	26.655407	7
CGCAGAG	30670	0.0	25.78908	8
TGGTATC	4885	0.0	23.77671	2
GTGGTAT	5485	0.0	22.953325	1
GCAGAGT	35355	0.0	22.407658	9
GTACATG	29400	0.0	21.079767	1
TACATGG	29425	0.0	20.500015	2
ACATGGG	29670	0.0	19.829414	3
GAGTACT	18050	0.0	19.819979	12-13
CAGAGTA	34640	0.0	19.089066	10-11
AGAGTAC	31920	0.0	18.730448	10-11
GTACTTT	19565	0.0	18.18786	14-15
CATGGGG	21625	0.0	17.386232	4
>>END_MODULE