FastQCFastQC Report
Fri 27 May 2016
SRR2139446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5230819
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82050.047.670611
GTATCAA157700.039.0296441
TCAACGC186250.032.1973344
ATCAACG187150.032.066213
TATCAAC191100.031.4046082
CAACGCA191200.031.3637735
AACGCAG195400.030.645686
ACGCAGA223800.026.8330067
CGCAGAG231950.025.8350128
GTACATG228400.023.1198231
GCAGAGT263450.022.6974229
TACATGG231750.022.1230342
ACATGGG227950.021.9857653
GAGTACT122600.020.36869212-13
CATGGGG158900.019.3258884
TGGTATC42850.019.1121852
CAGAGTA256700.019.10727310-11
AGAGTAC232050.019.10599510-11
CGTCGAA2757.743152E-418.99322394
GTACTTT131950.018.77999914-15
Produced by FastQC (version 0.11.5)