##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139446_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5230819
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.625418887558524	28.0	18.0	33.0	18.0	33.0
2	30.0795114493543	31.0	27.0	33.0	27.0	34.0
3	31.927410411256822	33.0	31.0	33.0	29.0	34.0
4	31.795169551842648	33.0	31.0	33.0	29.0	34.0
5	32.484507684169536	33.0	33.0	33.0	32.0	34.0
6	35.86073117804305	38.0	36.0	38.0	31.0	38.0
7	36.92411781023201	38.0	37.0	38.0	35.0	38.0
8	36.45286732345355	38.0	37.0	38.0	34.0	38.0
9	37.101349520983234	38.0	38.0	38.0	36.0	38.0
10-11	37.25527025500213	38.0	38.0	38.0	36.0	38.0
12-13	37.257945361902216	38.0	38.0	38.0	36.0	38.0
14-15	37.24556192825636	38.0	38.0	38.0	36.0	38.0
16-17	37.29612380011619	38.0	38.0	38.0	36.0	38.0
18-19	37.32519611173699	38.0	38.0	38.0	36.5	38.0
20-21	37.35854532681839	38.0	38.0	38.0	37.0	38.0
22-23	37.37315582904321	38.0	38.0	38.0	37.0	38.0
24-25	37.38344368528153	38.0	38.0	38.0	37.0	38.0
26-27	37.36048626787708	38.0	38.0	38.0	37.0	38.0
28-29	37.34432619965885	38.0	38.0	38.0	37.0	38.0
30-31	37.354913413020185	38.0	38.0	38.0	37.0	38.0
32-33	37.34405895494514	38.0	38.0	38.0	37.0	38.0
34-35	37.324290166232274	38.0	38.0	38.0	37.0	38.0
36-37	37.3062060015679	38.0	38.0	38.0	37.0	38.0
38-39	37.2949811999783	38.0	38.0	38.0	37.0	38.0
40-41	37.288790767333325	38.0	38.0	38.0	37.0	38.0
42-43	37.252379509585566	38.0	38.0	38.0	37.0	38.0
44-45	37.20724709159364	38.0	38.0	38.0	36.0	38.0
46-47	37.17188673474942	38.0	38.0	38.0	36.0	38.0
48-49	37.107891949595256	38.0	38.0	38.0	36.0	38.0
50-51	37.045050652613355	38.0	38.0	38.0	36.0	38.0
52-53	37.01889884732583	38.0	38.0	38.0	36.0	38.0
54-55	36.98291485854825	38.0	38.0	38.0	36.0	38.0
56-57	36.91217027977909	38.0	38.0	38.0	36.0	38.0
58-59	36.87582153723358	38.0	38.0	38.0	35.0	38.0
60-61	36.89426799753373	38.0	38.0	38.0	35.0	38.0
62-63	36.89969304157635	38.0	38.0	38.0	35.0	38.0
64-65	36.89985649810652	38.0	38.0	38.0	35.0	38.0
66-67	36.890457353253865	38.0	38.0	38.0	35.0	38.0
68-69	36.871565499101486	38.0	38.0	38.0	35.0	38.0
70-71	36.85339515661878	38.0	38.0	38.0	35.0	38.0
72-73	36.84622818449087	38.0	38.0	38.0	35.0	38.0
74-75	36.818675287076005	38.0	38.0	38.0	35.0	38.0
76-77	36.79144015324948	38.0	38.0	38.0	35.0	38.0
78-79	36.789457152211725	38.0	38.0	38.0	35.0	38.0
80-81	36.78465778503187	38.0	38.0	38.0	35.0	38.0
82-83	36.7203653306795	38.0	38.0	38.0	34.0	38.0
84-85	36.6905329834626	38.0	38.0	38.0	34.0	38.0
86-87	36.69016636714802	38.0	38.0	38.0	34.0	38.0
88-89	36.670589361943314	38.0	38.0	38.0	34.0	38.0
90-91	36.633525997769084	38.0	38.0	38.0	34.0	38.0
92-93	36.61480678313195	38.0	38.0	38.0	34.0	38.0
94-95	36.58872382672101	38.0	38.0	38.0	34.0	38.0
96-97	36.548952889945035	38.0	38.0	38.0	34.0	38.0
98-99	36.53772017223865	38.0	37.5	38.0	34.0	38.0
100	34.994068354852224	38.0	34.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	16.0
21	87.0
22	312.0
23	955.0
24	2188.0
25	4479.0
26	8082.0
27	13831.0
28	22100.0
29	33010.0
30	45098.0
31	58650.0
32	78528.0
33	108636.0
34	170784.0
35	328335.0
36	1037934.0
37	3317790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.95340844330496	16.636668177583662	13.440801526491358	21.96912185262002
2	14.762511800519318	20.182797643605006	43.17070634113738	21.883984214738287
3	18.64720959545027	26.872086089720952	29.786307032920266	24.694397281908508
4	12.843266035395223	18.321566852150685	38.55388611228949	30.2812810001646
5	14.072712910294335	37.60801847818066	34.749459835918586	13.569808775606415
6	30.062806728366493	37.3755952473126	19.04560078565285	13.515997238668053
7	27.661958863420814	32.679987588941614	22.55987064358373	17.09818290405384
8	25.16045766446899	36.34199539307325	20.720177853601893	17.777369088855874
9	26.561576686174764	17.738312107530387	21.289572435979913	34.410538770314936
10-11	25.0005496271234	26.862466470355788	28.035466339018804	20.101517563502007
12-13	26.038895629919523	24.33985003113279	28.384637281465867	21.236617057481823
14-15	23.16912514082403	25.408124425639656	26.43082278320087	24.991927650335445
16-17	22.305197331431273	28.696232846137477	26.94300835108231	22.05556147134894
18-19	22.270413103569442	27.775401901690728	28.891278784450392	21.06290621028944
20-21	23.46597934833229	26.761062055179703	28.672436760672053	21.100521835815954
22-23	23.159208367328112	26.576877431911267	28.613562638578017	21.650351562182607
24-25	22.70956371376637	26.84610838583711	28.712449995306162	21.73187790509036
26-27	22.527065291717722	26.93469795639264	28.759941532544897	21.77829521934474
28-29	22.517191965156318	26.88849078260467	28.779905832380337	21.814411419858676
30-31	22.769205764313725	26.86914626498108	28.61169372469651	21.74995424600868
32-33	22.523140643194186	27.08574563996403	28.602503361766544	21.78861035507523
34-35	22.7331238474464	27.01750939630314	28.582348981601047	21.667017774649413
36-37	22.73890230032192	26.8676790570951	28.583400711922902	21.810017930660077
38-39	22.545984551657167	27.069668403962105	28.688798923727443	21.69554812065329
40-41	22.786568602339397	26.966109261224343	28.537558393403835	21.709763743032433
42-43	22.626847870357068	27.008496022268186	28.37422175613007	21.990434351244676
44-45	22.66094471562253	27.102495421086847	28.317275390827923	21.9192844724627
46-47	22.806506968601123	27.116095180094966	28.014648803382315	22.062749047921596
48-49	22.769059404295895	27.194304835698834	27.96842780741772	22.06820795258755
50-51	22.700354608488997	27.37844953407888	27.832979482901877	22.08821637453024
52-53	22.941189228873245	27.285870932350313	27.69939583622777	22.073544002548672
54-55	22.93084987828013	27.17840625425896	27.735243463446206	22.155500404014706
56-57	22.837477158572117	27.35825007007338	27.75570339121908	22.048569380135422
58-59	22.827023708475043	27.304460523535724	27.8240956035435	22.04442016444573
60-61	22.91643891376403	27.30535452800964	27.813561562006058	21.96464499622027
62-63	22.889994127479845	27.3934143987713	27.81795422035286	21.898637253395993
64-65	22.887001709595715	27.317473761999384	27.787620644074895	22.007903884330002
66-67	23.103290453463675	27.341581057609982	27.53574484811732	22.01938364080902
68-69	22.886809075686447	27.38047043294291	27.801697161418964	21.931023329951675
70-71	23.063669081280207	27.059447926720193	27.848671697406147	22.028211294593458
72-73	23.1755402609674	27.10379514399615	27.68693060150347	22.03373399353299
74-75	22.76056828374283	27.25307830869628	27.80570900465673	22.180644402904157
76-77	22.73556512920678	27.23891700590082	27.81862151698194	22.206896347910458
78-79	22.793169675947265	27.1540656999638	27.71142038143663	22.341344242652298
80-81	22.779862631639265	27.24914823357354	27.65589193156916	22.315097203218038
82-83	22.837455725981346	27.13832019495576	27.759457943434313	22.264766135628577
84-85	22.782660758538228	27.175650060505856	27.728178250882777	22.313510930073132
86-87	22.601000875260596	27.253849849098565	27.834757879049487	22.31039139659135
88-89	22.753552306984854	27.257551106511336	27.711087489340603	22.277809097163203
90-91	22.785119870856153	27.26353263492809	27.697269876701785	22.254077617513975
92-93	22.851364552943647	27.282607983890927	27.62236090411169	22.243666559053736
94-95	22.673198372956197	27.31010323008991	27.646083475986305	22.370614920967586
96-97	22.83907090938492	27.38150509956185	27.713464720731807	22.06595927032143
98-99	23.847483200122703	29.35740589608554	27.866850578605174	18.92826032518658
100	30.6268093542153	40.23011014577614	29.143080500008566	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	519.0
1	527.5
2	852.5
3	1268.0
4	1658.5
5	2449.0
6	3112.5
7	3898.5
8	5077.5
9	5927.0
10	6194.5
11	6248.0
12	6749.5
13	8092.0
14	11137.0
15	14894.0
16	18027.0
17	20558.5
18	21084.5
19	19269.0
20	16562.5
21	14276.5
22	13792.0
23	15473.5
24	19293.0
25	25245.5
26	33659.5
27	43204.0
28	52268.5
29	63045.0
30	74279.5
31	85089.5
32	99706.0
33	116714.0
34	130789.5
35	144573.5
36	160791.5
37	173611.5
38	183233.5
39	190528.5
40	197367.5
41	202148.0
42	204658.5
43	209261.5
44	214395.0
45	218499.0
46	218840.5
47	219616.0
48	221947.0
49	220619.0
50	216664.5
51	211789.0
52	205678.5
53	196933.0
54	185080.5
55	172790.5
56	157719.5
57	141953.5
58	127384.0
59	110814.5
60	93968.0
61	76734.5
62	60467.0
63	46734.0
64	34837.0
65	25787.5
66	19199.5
67	14472.0
68	10900.0
69	7877.0
70	5431.5
71	3564.5
72	2292.5
73	1421.5
74	909.0
75	589.0
76	371.5
77	239.5
78	160.5
79	102.5
80	61.5
81	40.0
82	27.0
83	16.0
84	10.5
85	8.5
86	8.0
87	5.5
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00223674342392654
3	0.001720571864558877
4	0.0
5	1.9117465161765297E-5
6	1.9117465161765297E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.1987390518329788E-4
26-27	0.0020077706460489655
28-29	0.006981409653721124
30-31	0.002517363346175077
32-33	0.005877891361609219
34-35	0.002729341575176062
36-37	0.0012149694652138332
38-39	2.908634271138427E-5
40-41	1.073339743448383E-4
42-43	0.0022588241209810485
44-45	5.64012053234426E-4
46-47	3.1937115818952484E-4
48-49	0.002023533595036967
50-51	4.4671048490423136E-4
52-53	0.0
54-55	0.004422420082281754
56-57	0.04530883164734598
58-59	0.06690578805797175
60-61	0.03297584836182961
62-63	0.013909190148328457
64-65	0.0016550787865865107
66-67	0.0
68-69	0.0021444923964615
70-71	0.0038113557854226577
72-73	0.012771748223667332
74-75	0.01583969820140058
76-77	0.02144404689052797
78-79	0.0069100639112040715
80-81	0.008223515380003543
82-83	0.03218679122540717
84-85	0.018765786644016697
86-87	0.012973555708448213
88-89	0.006173551707119942
90-91	4.886627194935499E-5
92-93	0.0
94-95	0.0
96-97	3.810808112346688E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	31.0
20-21	139.0
22-23	303.0
24-25	489.0
26-27	1142.0
28-29	2911.0
30-31	8864.0
32-33	10997.0
34-35	14883.0
36-37	25709.0
38-39	33189.0
40-41	32345.0
42-43	36470.0
44-45	42403.0
46-47	43687.0
48-49	42388.0
50-51	38686.0
52-53	36303.0
54-55	38600.0
56-57	39534.0
58-59	40805.0
60-61	40238.0
62-63	38486.0
64-65	39465.0
66-67	42032.0
68-69	43601.0
70-71	44227.0
72-73	41414.0
74-75	41019.0
76-77	43216.0
78-79	45172.0
80-81	45410.0
82-83	43052.0
84-85	41673.0
86-87	42397.0
88-89	44919.0
90-91	46497.0
92-93	45573.0
94-95	53010.0
96-97	162220.0
98-99	1228724.0
100-101	2568596.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.66642586354038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.33854557317682	80.07323833974509
2	6.319536471902867	11.080223512120247
3	1.3736545181236999	3.6127014592562587
4	0.4480301872933851	1.571088207959346
5	0.21108609288624644	0.925258165641826
6	0.10477575066661798	0.5511189346867114
7	0.05942810222848261	0.3646894522756918
8	0.0329174598752854	0.2308604844618219
9	0.026153791705098153	0.2063528497408918
>10	0.08319138443746159	1.1191173335957831
>50	0.0019598154480762586	0.11960282756711388
>100	6.563320288447993E-4	0.10298712479620675
>500	6.45202270928672E-5	0.04276130815301361
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.91174651617653E-5	0.0	0.0	0.0
3	0.0	1.91174651617653E-5	0.0	3.82349303235306E-5	0.0
4	0.0	7.64698606470612E-5	0.0	3.82349303235306E-5	7.64698606470612E-5
5	0.0	7.64698606470612E-5	0.0	3.82349303235306E-5	7.64698606470612E-5
6	0.0	7.64698606470612E-5	0.0	3.82349303235306E-5	7.64698606470612E-5
7	0.0	7.64698606470612E-5	0.0	3.82349303235306E-5	7.64698606470612E-5
8	0.0	7.64698606470612E-5	0.0	3.82349303235306E-5	9.55873258088265E-5
9	0.0	1.1470479097059179E-4	0.0	9.55873258088265E-5	9.55873258088265E-5
10-11	0.0	1.1470479097059179E-4	0.0	1.2426352355147444E-4	9.55873258088265E-5
12-13	0.0	1.1470479097059179E-4	0.0	1.338222561323571E-4	1.0514605838970914E-4
14-15	0.0	1.7205718645588769E-4	0.0	1.4338098871323975E-4	1.1470479097059179E-4
16-17	0.0	1.7205718645588769E-4	0.0	2.102921167794183E-4	1.1470479097059179E-4
18-19	0.0	2.0073338419853565E-4	0.0	3.058794425882448E-4	1.1470479097059179E-4
20-21	0.0	2.3896831452206623E-4	0.0	4.014667683970713E-4	1.1470479097059179E-4
22-23	0.0	2.676445122647142E-4	0.0	4.205842335588366E-4	1.2426352355147444E-4
24-25	0.0	2.867619774264795E-4	0.0	4.3970169872060185E-4	1.338222561323571E-4
26-27	0.0	2.867619774264795E-4	0.0	4.7793662904413247E-4	1.338222561323571E-4
28-29	0.0	2.867619774264795E-4	0.0	5.926414200147243E-4	1.338222561323571E-4
30-31	0.0	3.632318380735407E-4	0.0	0.0012999876310000403	1.338222561323571E-4
32-33	0.0	3.632318380735407E-4	0.0	0.0028676197742647947	1.338222561323571E-4
34-35	0.0	4.4926043130148454E-4	0.0	0.004769807557860443	1.6249845387500503E-4
36-37	0.0	5.352890245294284E-4	0.0	0.007952865507294364	1.7205718645588769E-4
38-39	0.0	5.544064896911936E-4	0.0	0.012741790530316571	1.7205718645588769E-4
40-41	0.0	6.691112806617855E-4	0.0	0.018429236415941748	1.7205718645588769E-4
42-43	0.0	6.691112806617855E-4	0.0	0.023495364683809555	1.7205718645588769E-4
44-45	0.0	7.838160716323772E-4	0.0	0.028905607324589133	1.7205718645588769E-4
46-47	0.0	9.080795951838516E-4	0.0	0.034602611942795194	1.8161591903677034E-4
48-49	0.0	9.27197060345617E-4	0.0	0.04061505473617038	2.102921167794183E-4
50-51	0.0	0.001032343118735326	0.0	0.04705764049568528	2.102921167794183E-4
52-53	0.0	0.001032343118735326	0.0	0.05297449596325164	2.102921167794183E-4
54-55	0.0	0.0010419018513162087	0.0	0.058394297336612104	2.102921167794183E-4
56-57	0.0	0.0010514605838970914	0.0	0.06493247042193584	2.102921167794183E-4
58-59	0.0	0.0010514605838970914	0.0	0.07038094799303896	2.102921167794183E-4
60-61	9.55873258088265E-6	0.0010801367816397395	0.0	0.07665147656609797	2.102921167794183E-4
62-63	1.91174651617653E-5	0.0010992542468015046	0.0	0.08236759864946579	2.2940958194118358E-4
64-65	1.91174651617653E-5	0.0013573400264853363	0.0	0.08818886679122331	2.2940958194118358E-4
66-67	1.91174651617653E-5	0.0013668987590662188	0.0	0.0941535159216941	2.2940958194118358E-4
68-69	1.91174651617653E-5	0.0013764574916471015	0.0	0.09978360941183398	2.2940958194118358E-4
70-71	1.91174651617653E-5	0.0013860162242279842	0.0	0.1047254741561503	2.3896831452206623E-4
72-73	1.91174651617653E-5	0.0014051336893897495	0.0	0.10904602128270927	2.867619774264795E-4
74-75	1.91174651617653E-5	0.0014529273522941627	0.0	0.11211437444117259	2.867619774264795E-4
76-77	1.91174651617653E-5	0.001529397212941224	0.0	0.1151444926693124	2.867619774264795E-4
78-79	1.91174651617653E-5	0.0015867496084265198	0.0	0.11730476623259187	2.867619774264795E-4
80-81	1.91174651617653E-5	0.0015963083410074025	0.0	0.1180025537109963	2.867619774264795E-4
82-83	2.867619774264795E-5	0.0016154258061691676	0.0	0.11853784273552573	2.867619774264795E-4
84-85	3.82349303235306E-5	0.001634543271330933	0.0	0.11878636978262869	2.867619774264795E-4
86-87	3.82349303235306E-5	0.0017014543993971116	0.0	0.11890107457359927	2.867619774264795E-4
88	3.82349303235306E-5	0.001854394120691234	0.0	0.11892975077134192	3.058794425882448E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8205	0.0	47.67061	1
GTATCAA	15770	0.0	39.029644	1
TCAACGC	18625	0.0	32.197334	4
ATCAACG	18715	0.0	32.06621	3
TATCAAC	19110	0.0	31.404608	2
CAACGCA	19120	0.0	31.363773	5
AACGCAG	19540	0.0	30.64568	6
ACGCAGA	22380	0.0	26.833006	7
CGCAGAG	23195	0.0	25.835012	8
GTACATG	22840	0.0	23.119823	1
GCAGAGT	26345	0.0	22.697422	9
TACATGG	23175	0.0	22.123034	2
ACATGGG	22795	0.0	21.985765	3
GAGTACT	12260	0.0	20.368692	12-13
CATGGGG	15890	0.0	19.325888	4
TGGTATC	4285	0.0	19.112185	2
CAGAGTA	25670	0.0	19.107273	10-11
AGAGTAC	23205	0.0	19.105995	10-11
CGTCGAA	275	7.743152E-4	18.993223	94
GTACTTT	13195	0.0	18.779999	14-15
>>END_MODULE