FastQCFastQC Report
Fri 27 May 2016
SRR2139445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7832614
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113500.054.783471
GTATCAA220950.041.2992131
TCAACGC274000.032.580774
TATCAAC276850.032.4794732
ATCAACG278500.032.0701563
CAACGCA284300.031.3852885
AACGCAG290050.030.7927076
ACGCAGA331250.027.001687
CGCAGAG347050.025.784768
GTGGTAT52600.024.2464161
GTACATG358550.023.2103061
CTTATAC50400.022.9191551
GCAGAGT394000.022.8320679
TGGTATC52500.022.3295612
TACATGG368300.022.059532
ACATGGG368150.021.4146443
TATACAC68200.020.651543
GAGTACT176600.020.52150312-13
CAGAGTA384850.019.64273610-11
CATGGGG275250.019.6406484
Produced by FastQC (version 0.11.5)