##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7832614
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.6357254423619	28.0	18.0	33.0	18.0	33.0
2	30.021778680782685	31.0	27.0	33.0	27.0	34.0
3	31.876327238901343	33.0	31.0	33.0	29.0	34.0
4	31.7218167523639	33.0	31.0	33.0	29.0	34.0
5	32.44040010142208	33.0	33.0	33.0	32.0	34.0
6	35.83202108517029	38.0	36.0	38.0	31.0	38.0
7	36.888549595320285	38.0	37.0	38.0	35.0	38.0
8	36.41259201589661	38.0	37.0	38.0	34.0	38.0
9	37.07275476616108	38.0	38.0	38.0	36.0	38.0
10-11	37.22758838875502	38.0	38.0	38.0	36.0	38.0
12-13	37.233308382105896	38.0	38.0	38.0	36.0	38.0
14-15	37.218540068488	38.0	38.0	38.0	36.0	38.0
16-17	37.27225004321674	38.0	38.0	38.0	36.0	38.0
18-19	37.297731063984514	38.0	38.0	38.0	36.5	38.0
20-21	37.33332472265934	38.0	38.0	38.0	37.0	38.0
22-23	37.34748967127601	38.0	38.0	38.0	37.0	38.0
24-25	37.35693869509247	38.0	38.0	38.0	37.0	38.0
26-27	37.33539062443529	38.0	38.0	38.0	37.0	38.0
28-29	37.317258855140594	38.0	38.0	38.0	37.0	38.0
30-31	37.326699002954484	38.0	38.0	38.0	37.0	38.0
32-33	37.316361112555086	38.0	38.0	38.0	37.0	38.0
34-35	37.29310257844067	38.0	38.0	38.0	37.0	38.0
36-37	37.27761938150397	38.0	38.0	38.0	37.0	38.0
38-39	37.26014187971937	38.0	38.0	38.0	37.0	38.0
40-41	37.26387087436592	38.0	38.0	38.0	37.0	38.0
42-43	37.226571674534284	38.0	38.0	38.0	36.0	38.0
44-45	37.18402667749221	38.0	38.0	38.0	36.0	38.0
46-47	37.14823506006769	38.0	38.0	38.0	36.0	38.0
48-49	37.086646512789635	38.0	38.0	38.0	36.0	38.0
50-51	37.02143703935147	38.0	38.0	38.0	36.0	38.0
52-53	36.99207867877013	38.0	38.0	38.0	36.0	38.0
54-55	36.95317458149232	38.0	38.0	38.0	36.0	38.0
56-57	36.88152461218006	38.0	38.0	38.0	35.0	38.0
58-59	36.84214595563424	38.0	38.0	38.0	35.0	38.0
60-61	36.8615017235723	38.0	38.0	38.0	35.0	38.0
62-63	36.86330062312608	38.0	38.0	38.0	35.0	38.0
64-65	36.8617751174165	38.0	38.0	38.0	35.0	38.0
66-67	36.85034850383339	38.0	38.0	38.0	35.0	38.0
68-69	36.826431123072425	38.0	38.0	38.0	35.0	38.0
70-71	36.81227235130107	38.0	38.0	38.0	35.0	38.0
72-73	36.799577230942774	38.0	38.0	38.0	35.0	38.0
74-75	36.769543091715974	38.0	38.0	38.0	35.0	38.0
76-77	36.74215925751144	38.0	38.0	38.0	34.5	38.0
78-79	36.74141426350482	38.0	38.0	38.0	34.0	38.0
80-81	36.73308629385626	38.0	38.0	38.0	34.0	38.0
82-83	36.6685567555501	38.0	38.0	38.0	34.0	38.0
84-85	36.637507320310476	38.0	38.0	38.0	34.0	38.0
86-87	36.63562181571638	38.0	38.0	38.0	34.0	38.0
88-89	36.61391104865119	38.0	38.0	38.0	34.0	38.0
90-91	36.57432404505239	38.0	38.0	38.0	34.0	38.0
92-93	36.55681655964973	38.0	38.0	38.0	34.0	38.0
94-95	36.52889302601018	38.0	38.0	38.0	34.0	38.0
96-97	36.49488969393579	38.0	37.0	38.0	34.0	38.0
98-99	36.48855941515009	38.0	37.0	38.0	34.0	38.0
100	34.940060155963934	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	11.0
20	26.0
21	141.0
22	554.0
23	1590.0
24	3559.0
25	7185.0
26	12939.0
27	21709.0
28	34392.0
29	51198.0
30	69666.0
31	93397.0
32	123769.0
33	173049.0
34	270356.0
35	513876.0
36	1555559.0
37	4899636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.89464921927724	16.510260814588847	13.40113275082878	22.193957215305133
2	14.964615765477005	19.955089363561278	42.78689292727697	22.29340194368475
3	18.541314472650892	26.74905383999309	29.663506267324564	25.046125420031462
4	13.241288285111457	18.341871564205768	37.37080622126917	31.046033929413603
5	14.46838097793954	37.342783906924524	34.329969916800415	13.858865198335524
6	30.74690987225834	36.6747078398566	18.698593890538334	13.879788397346731
7	28.13991599739244	32.398481528644204	22.10482988182489	17.356772592138462
8	25.7078160624282	36.03914350943376	20.392898207418366	17.860142220719673
9	26.87017386532772	17.479975395187353	20.764064206406697	34.885786533078225
10-11	25.51933492445817	26.45322493869863	27.570450937579714	20.45698919926349
12-13	26.51766064304969	23.964119768955804	27.88972493729424	21.628494650700265
14-15	23.609538271642137	24.878469690961406	25.718872396877977	25.79311964051848
16-17	22.926656413810257	28.208009484445423	26.259190099244005	22.606144002500315
18-19	22.993428502923802	27.25526880298199	28.092907425286118	21.65839526880809
20-21	23.961785078089267	26.19589360670561	27.95049627882645	21.891825036378673
22-23	23.903555348465108	26.12461316570498	27.879513194451228	22.092318291378685
24-25	23.40937635283985	26.40113908436958	27.881334610247098	22.30814995254347
26-27	23.184881845281268	26.47687652086147	28.05076382745284	22.287477806404425
28-29	23.14948343388922	26.29313328941012	28.106140981252075	22.45124229544858
30-31	23.376408812441305	26.309818005930335	27.84649746068312	22.46727572094524
32-33	23.212917026037356	26.558388462445276	27.901972318518524	22.326722192998847
34-35	23.40544791106111	26.507321885224293	27.90255616566812	22.184674038046477
36-37	23.325692254588667	26.37373134528057	27.819433907477766	22.481142492652996
38-39	23.195631097738534	26.52968040366634	28.004530083356403	22.270158415238726
40-41	23.367748687409843	26.315364506454337	27.884368154941715	22.432518651194115
42-43	23.24950932190288	26.465540310941982	27.751577324406824	22.533373042748313
44-45	23.20785184899401	26.537471720266954	27.716696180581046	22.537980250157993
46-47	23.452166474597195	26.552378697646915	27.323312898415864	22.67214192934002
48-49	23.379815744105354	26.651797770347713	27.17038781429097	22.797998671255964
50-51	23.30107562461389	26.81219891706344	27.164506766651886	22.722218691670786
52-53	23.513293449782786	26.67397671286959	27.18858704331413	22.624142794033496
54-55	23.446871396996396	26.623753623846014	27.10243887638496	22.826936102772628
56-57	23.42434966310832	26.851757815310634	27.08699019128364	22.636902330297406
58-59	23.355760472079485	26.899763742367526	27.189730485048084	22.5547453005049
60-61	23.53296793312123	27.04809727653149	26.99562754574163	22.42330724460565
62-63	23.432588162203118	27.18883331378934	27.008265136071298	22.370313387936243
64-65	23.523491482016247	27.08036314799282	26.92334844437689	22.472796925614034
66-67	23.661971548530946	27.094917229804622	26.781611722771327	22.461499498893108
68-69	23.385767612648568	27.08731490546627	27.03554016016339	22.491377321721775
70-71	23.62553335027204	26.768883009015482	27.10501414966225	22.50056949105023
72-73	23.81075093457303	26.81633455022415	26.823363300541843	22.549551214660983
74-75	23.356738996156576	26.85502947705843	27.083292722613585	22.704938804171416
76-77	23.44208898891491	26.73861914798416	27.095989577183715	22.72330228591721
78-79	23.307068863502202	26.62346412997394	27.047371567090977	23.02209543943288
80-81	23.35112934397341	26.829199450301232	27.00727174428696	22.8123994614384
82-83	23.39412004903114	26.699603910901782	27.080904858649156	22.825371181417925
84-85	23.30340547497722	26.748923762917087	27.02351092106243	22.924159841043263
86-87	23.22041664065906	26.805758882774665	27.155403425384645	22.818421051181627
88-89	23.345495777687695	26.729321811474932	27.0189364911556	22.906245919681776
90-91	23.356687987082804	26.74818231501272	27.037458933159076	22.8576707647454
92-93	23.419605347739417	26.738072125654995	26.99653916329565	22.845783363309938
94-95	23.28457702835809	26.710359349735747	27.079429269901222	22.925634352004938
96-97	23.44535263089167	26.76904985975282	27.007767754205624	22.777829755149888
98-99	24.44904447093674	28.808614211914307	27.163948195513164	19.578393121635777
100	31.737466665319157	39.69796077657011	28.56457255811073	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	919.0
1	888.5
2	1289.0
3	1897.5
4	2440.0
5	3607.5
6	4668.5
7	5774.0
8	7361.0
9	8414.5
10	8680.0
11	8675.0
12	9434.5
13	11482.0
14	15673.5
15	21018.0
16	25537.5
17	28787.5
18	29082.5
19	26416.5
20	22833.0
21	19600.5
22	18169.5
23	19757.5
24	24225.5
25	31381.0
26	40467.0
27	50847.0
28	63190.5
29	79290.0
30	95495.0
31	111280.0
32	131629.0
33	153805.0
34	170586.5
35	187299.0
36	208132.5
37	225533.5
38	241726.5
39	253545.0
40	262277.0
41	272006.5
42	279529.0
43	288838.5
44	303839.5
45	319758.0
46	325139.0
47	326209.0
48	329875.5
49	332832.0
50	334894.5
51	335901.0
52	332775.5
53	322897.0
54	308256.5
55	293507.5
56	272330.0
57	247045.5
58	222841.0
59	192402.5
60	161967.5
61	134263.5
62	108545.0
63	85047.5
64	63931.5
65	47519.5
66	35338.5
67	26737.5
68	20083.5
69	14579.0
70	10267.0
71	6785.5
72	4248.5
73	2601.0
74	1600.0
75	969.0
76	616.5
77	402.0
78	264.5
79	160.0
80	108.0
81	78.0
82	53.5
83	37.0
84	24.5
85	15.5
86	8.5
87	5.0
88	2.5
89	1.5
90	1.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002183179204286079
3	0.002106576425188322
4	0.0
5	3.830138954887857E-5
6	1.1490416864663573E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.2344451336466325E-4
26-27	0.0017302532611922004
28-29	0.0064501139882025505
30-31	0.002281632582889251
32-33	0.0051210301054477715
34-35	0.0024564961270420265
36-37	0.0010292064342384045
38-39	3.2318945068264726E-5
40-41	3.900663873487949E-5
42-43	0.0019291916052829507
44-45	4.542776162934238E-4
46-47	2.9858706609742257E-4
48-49	0.0015983730034130948
50-51	4.6499729492877995E-4
52-53	0.0
54-55	0.004626117776311967
56-57	0.04380629263456894
58-59	0.06489453320768657
60-61	0.03177659918391598
62-63	0.013077731329283747
64-65	0.0013564954577643066
66-67	0.0
68-69	0.002175908857611705
70-71	0.0034825355224098967
72-73	0.012263775748859113
74-75	0.015101186878898597
76-77	0.020045055220337258
78-79	0.005923339115027382
80-81	0.007778584020830391
82-83	0.03135124563851574
84-85	0.018018192214737556
86-87	0.013497207534007453
88-89	0.0057692321446039755
90-91	5.620916554683288E-5
92-93	0.0
94-95	0.0
96-97	8.33861515114699E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	41.0
20-21	156.0
22-23	396.0
24-25	565.0
26-27	1364.0
28-29	3917.0
30-31	11874.0
32-33	14403.0
34-35	19294.0
36-37	34136.0
38-39	44593.0
40-41	44498.0
42-43	48908.0
44-45	57774.0
46-47	59662.0
48-49	58013.0
50-51	53578.0
52-53	49620.0
54-55	51502.0
56-57	54227.0
58-59	56391.0
60-61	57906.0
62-63	55475.0
64-65	58036.0
66-67	63183.0
68-69	65617.0
70-71	72108.0
72-73	59690.0
74-75	58281.0
76-77	60445.0
78-79	63535.0
80-81	64279.0
82-83	61333.0
84-85	59610.0
86-87	60433.0
88-89	64321.0
90-91	66619.0
92-93	66497.0
94-95	78683.0
96-97	252923.0
98-99	1919759.0
100-101	3858969.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.52429823285141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.38716516180331	72.94088777768951
2	7.915216988795228	13.06395454722139
3	1.977152287949948	4.89489315187638
4	0.7777010782346858	2.5671694286499727
5	0.3540713521912341	1.4609744931969184
6	0.18046966294977512	0.8935879372349649
7	0.11356856748761257	0.6560516433257816
8	0.0677018573493371	0.4469638613451727
9	0.05270653252305553	0.39146126478769055
>10	0.1675871163696479	2.174240391667661
>50	0.004812947867763764	0.26345096955787684
>100	0.0018464464784489869	0.24636453344670686
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.553425969925238E-5	0.0	0.0	0.0
4	0.0	7.660277909775714E-5	0.0	0.0	0.0
5	0.0	7.660277909775714E-5	0.0	0.0	0.0
6	8.936990894738333E-5	7.660277909775714E-5	0.0	0.0	0.0
7	8.936990894738333E-5	7.660277909775714E-5	0.0	0.0	0.0
8	8.936990894738333E-5	1.276712984962619E-4	0.0	0.0	0.0
9	8.936990894738333E-5	3.064111163910286E-4	0.0	0.0	0.0
10-11	1.0213703879700952E-4	3.064111163910286E-4	0.0	1.276712984962619E-5	0.0
12-13	1.0213703879700952E-4	3.064111163910286E-4	0.0	1.276712984962619E-5	0.0
14-15	1.0213703879700952E-4	3.8301389548878574E-4	0.0	1.276712984962619E-5	0.0
16-17	1.468219932707012E-4	3.957810253384119E-4	0.0	1.276712984962619E-5	0.0
18-19	1.532055581955143E-4	4.149317201128512E-4	0.0	1.276712984962619E-5	0.0
20-21	1.532055581955143E-4	4.340824148872905E-4	0.0	1.276712984962619E-5	0.0
22-23	1.532055581955143E-4	4.723838044361691E-4	0.0	1.276712984962619E-5	0.0
24-25	1.9789051266920596E-4	5.106851939850476E-4	0.0	2.553425969925238E-5	0.0
26-27	2.0427407759401905E-4	5.234523238346738E-4	0.0	7.660277909775714E-5	0.0
28-29	2.0427407759401905E-4	5.234523238346738E-4	0.0	2.3619190221808455E-4	0.0
30-31	2.1704120744364525E-4	6.383564924813096E-4	0.0	8.553976999249547E-4	0.0
32-33	2.2342477236845834E-4	6.63890752180562E-4	0.0	0.002183179204286079	0.0
34-35	2.3619190221808455E-4	7.787949208271977E-4	0.0	0.0036641662668427167	0.0
36-37	2.489590320677107E-4	8.936990894738334E-4	0.0	0.005898413990527301	0.0
38-39	2.553425969925238E-4	9.32000479022712E-4	0.0	0.009990279107332495	0.0
40-41	2.553425969925238E-4	0.0010341375178197214	0.0	0.01478433636586713	0.0
42-43	2.553425969925238E-4	0.0010724389073686002	0.0	0.019284749637860362	0.0
44-45	2.617261619173369E-4	0.0011937266409400489	0.0	0.024117108285943878	0.0
46-47	2.6810972684215E-4	0.0012767129849626192	0.0	0.029032453278049958	0.0
48-49	2.6810972684215E-4	0.0012830965498874323	0.0	0.03386481192613347	0.0
50-51	2.6810972684215E-4	0.0015575898416543953	0.0	0.03938021202117199	0.0
52-53	2.6810972684215E-4	0.0015703569715040215	0.0	0.04480624220726312	0.0
54-55	2.6810972684215E-4	0.0015767405364288346	0.0	0.0499641626665121	0.0
56-57	2.808768566917762E-4	0.0015831241013536477	0.0	0.05509654886606183	0.0
58-59	2.808768566917762E-4	0.0015831241013536477	0.0	0.06057364757155147	0.0
60-61	2.936439865414024E-4	0.001602274796128087	0.0	0.06626778748448475	0.0
62-63	2.936439865414024E-4	0.0016916447050754704	0.0	0.07168743410565107	0.0
64-65	2.936439865414024E-4	0.0018256995684965453	0.0	0.07753477957677986	0.0
66-67	2.936439865414024E-4	0.0018320831334213584	0.0	0.08283952202929953	0.0
68-69	2.936439865414024E-4	0.0018895352177446764	0.0	0.08795914109899965	0.0
70-71	2.936439865414024E-4	0.001927836607293555	0.0	0.09212122542997778	0.0
72-73	3.064111163910286E-4	0.0019597544319176203	0.0	0.09556196692445204	0.0
74-75	3.064111163910286E-4	0.001991672256541686	0.0	0.09832605053689611	0.0
76-77	3.064111163910286E-4	0.002068275035639443	0.0	0.10090501076652061	0.0
78-79	3.191782462406548E-4	0.0021065764251883214	0.0	0.10278177885441565	0.0
80-81	3.191782462406548E-4	0.0021065764251883214	0.0	0.10364994368419023	0.0
82-83	3.255618111654679E-4	0.0021065764251883214	0.0	0.10432660156622042	0.0
84-85	3.31945376090281E-4	0.0021193435550379476	0.0	0.10463939624753626	0.0
86-87	3.31945376090281E-4	0.0021704120744364527	0.0	0.10473514972140846	0.0
88	3.31945376090281E-4	0.002272549113233462	0.0	0.10474153328633327	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11350	0.0	54.78347	1
GTATCAA	22095	0.0	41.299213	1
TCAACGC	27400	0.0	32.58077	4
TATCAAC	27685	0.0	32.479473	2
ATCAACG	27850	0.0	32.070156	3
CAACGCA	28430	0.0	31.385288	5
AACGCAG	29005	0.0	30.792707	6
ACGCAGA	33125	0.0	27.00168	7
CGCAGAG	34705	0.0	25.78476	8
GTGGTAT	5260	0.0	24.246416	1
GTACATG	35855	0.0	23.210306	1
CTTATAC	5040	0.0	22.919155	1
GCAGAGT	39400	0.0	22.832067	9
TGGTATC	5250	0.0	22.329561	2
TACATGG	36830	0.0	22.05953	2
ACATGGG	36815	0.0	21.414644	3
TATACAC	6820	0.0	20.65154	3
GAGTACT	17660	0.0	20.521503	12-13
CAGAGTA	38485	0.0	19.642736	10-11
CATGGGG	27525	0.0	19.640648	4
>>END_MODULE