FastQCFastQC Report
Fri 27 May 2016
SRR2139444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4297674
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61800.050.7161981
GTATCAA117000.040.2011571
TCAACGC135400.034.0390364
ATCAACG135850.033.9594233
TATCAAC138300.033.5756262
CAACGCA138700.033.229175
AACGCAG142500.032.3430566
ACGCAGA162900.028.3190487
CGCAGAG168850.027.3719148
TGGTATC26300.025.5953872
GTGGTAT28850.025.2650931
GCAGAGT192900.024.0037449
GTACATG148500.021.2793731
GAGTACT111000.021.14699412-13
TACATGG148850.020.5380152
ACATGGG147850.020.2124583
CAGAGTA190500.020.15385210-11
GTACTTT120150.019.4832514-15
AGAGTAC176500.018.84970310-11
GTCAACG5650.018.2129611
Produced by FastQC (version 0.11.5)