##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139444_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4297674
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.608844225969676	27.0	18.0	33.0	18.0	33.0
2	30.04090584814018	31.0	27.0	33.0	27.0	34.0
3	31.88420038374246	33.0	31.0	33.0	29.0	34.0
4	31.744955061738047	33.0	31.0	33.0	29.0	34.0
5	32.45726990925789	33.0	33.0	33.0	32.0	34.0
6	35.82577342999958	38.0	36.0	38.0	31.0	38.0
7	36.8737121522014	38.0	37.0	38.0	35.0	38.0
8	36.403682317458234	38.0	37.0	38.0	34.0	38.0
9	37.05407785699892	38.0	38.0	38.0	36.0	38.0
10-11	37.204956448534716	38.0	38.0	38.0	36.0	38.0
12-13	37.21617426077455	38.0	38.0	38.0	36.0	38.0
14-15	37.20398662160043	38.0	38.0	38.0	36.0	38.0
16-17	37.24949600644442	38.0	38.0	38.0	36.0	38.0
18-19	37.28301018178671	38.0	38.0	38.0	36.0	38.0
20-21	37.31778534389117	38.0	38.0	38.0	36.5	38.0
22-23	37.33122913133832	38.0	38.0	38.0	37.0	38.0
24-25	37.34250153909245	38.0	38.0	38.0	37.0	38.0
26-27	37.317761239900456	38.0	38.0	38.0	37.0	38.0
28-29	37.29966293571893	38.0	38.0	38.0	37.0	38.0
30-31	37.30767399883799	38.0	38.0	38.0	37.0	38.0
32-33	37.2960091121045	38.0	38.0	38.0	37.0	38.0
34-35	37.27494596443508	38.0	38.0	38.0	37.0	38.0
36-37	37.25526384099997	38.0	38.0	38.0	36.5	38.0
38-39	37.24201518856634	38.0	38.0	38.0	36.5	38.0
40-41	37.23264254453515	38.0	38.0	38.0	36.5	38.0
42-43	37.19310087002258	38.0	38.0	38.0	36.0	38.0
44-45	37.14796154687228	38.0	38.0	38.0	36.0	38.0
46-47	37.11127515990722	38.0	38.0	38.0	36.0	38.0
48-49	37.05307110103348	38.0	38.0	38.0	36.0	38.0
50-51	36.98707937097883	38.0	38.0	38.0	36.0	38.0
52-53	36.959703360716944	38.0	38.0	38.0	36.0	38.0
54-55	36.92197683922157	38.0	38.0	38.0	36.0	38.0
56-57	36.848306557412926	38.0	38.0	38.0	35.0	38.0
58-59	36.81252591389522	38.0	38.0	38.0	35.0	38.0
60-61	36.83350326392181	38.0	38.0	38.0	35.0	38.0
62-63	36.837466042181134	38.0	38.0	38.0	35.0	38.0
64-65	36.83794870353037	38.0	38.0	38.0	35.0	38.0
66-67	36.82862558239487	38.0	38.0	38.0	35.0	38.0
68-69	36.80939195729071	38.0	38.0	38.0	35.0	38.0
70-71	36.79071621760802	38.0	38.0	38.0	35.0	38.0
72-73	36.781921731239635	38.0	38.0	38.0	35.0	38.0
74-75	36.75542558594533	38.0	38.0	38.0	35.0	38.0
76-77	36.72578359773464	38.0	38.0	38.0	34.0	38.0
78-79	36.726171673541195	38.0	38.0	38.0	34.0	38.0
80-81	36.71616877332678	38.0	38.0	38.0	34.0	38.0
82-83	36.6512999744844	38.0	38.0	38.0	34.0	38.0
84-85	36.62185387596094	38.0	38.0	38.0	34.0	38.0
86-87	36.61885724322355	38.0	38.0	38.0	34.0	38.0
88-89	36.59930068762844	38.0	38.0	38.0	34.0	38.0
90-91	36.55967047203116	38.0	38.0	38.0	34.0	38.0
92-93	36.540437819258784	38.0	38.0	38.0	34.0	38.0
94-95	36.51343749011461	38.0	37.5	38.0	34.0	38.0
96-97	36.47568951456856	38.0	37.0	38.0	34.0	38.0
98-99	36.46566261097911	38.0	37.0	38.0	34.0	38.0
100	34.824780600251664	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	22.0
21	93.0
22	297.0
23	860.0
24	2090.0
25	4181.0
26	7462.0
27	12828.0
28	20460.0
29	29585.0
30	40057.0
31	51782.0
32	69224.0
33	96876.0
34	150330.0
35	283450.0
36	864150.0
37	2663923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.88548410139997	16.399778112532502	13.68007438442283	22.034663401644703
2	14.723408612046068	19.94852923211525	43.30939353148338	22.018668624355307
3	18.79428630464789	26.095703559471744	29.850819200652268	25.259190935228094
4	12.892159805513401	18.220553722781208	38.5295161987624	30.35777027294299
5	14.183462961592713	37.05678932371325	35.05882484339203	13.700922871302012
6	30.33165397452388	37.25237756627309	18.870006831605576	13.545961627597453
7	27.439028646658635	32.529759120863986	22.58198271902429	17.44922951345309
8	24.654150128650986	36.745295245753866	20.733052344128474	17.86750228146667
9	25.80293433145464	17.93102501492668	21.41079104650562	34.85524960711306
10-11	24.760486719095027	26.852199585170954	28.227676180184908	20.159637515549107
12-13	25.696237080802316	24.259238834774347	28.62823006119124	21.4162940232321
14-15	22.722454053052886	25.561896970314642	26.59587488487959	25.119774091752888
16-17	22.050357937805426	28.854701403596454	27.160610600059478	21.93433005853864
18-19	22.044622277073596	27.817081984347812	29.14690597751249	20.991389761066102
20-21	22.9115829432144	26.85633516775909	29.155118610971048	21.076963278055462
22-23	22.736161771293276	26.671274076918156	29.032141340453876	21.560422811334696
24-25	22.34149664185804	26.85628087915704	29.119426887034223	21.6827955919507
26-27	22.290665058857385	26.96911542210987	29.091572628559863	21.648646890472882
28-29	22.246103413071392	26.97301928590803	29.13657524088468	21.644302060135896
30-31	22.496576238190134	26.84591586429379	29.050533035005376	21.606974862510697
32-33	22.23960334470738	27.063061300805646	28.99885901613017	21.698476338356805
34-35	22.478266021625952	26.995309585269638	28.99883970811506	21.52758468498935
36-37	22.396931641293026	26.936427827441562	28.9516198510423	21.715020680223116
38-39	22.264305110551465	27.13269279492831	29.007789340049705	21.59521275447052
40-41	22.543605291393135	26.98410323865658	28.831204017638612	21.641087452311673
42-43	22.381973361853895	27.09416205441702	28.688885750735178	21.83497883299391
44-45	22.438954061413355	27.16976608895133	28.599881724283755	21.79139812535156
46-47	22.483935312819163	27.220743881946923	28.31750582115447	21.977814984079437
48-49	22.497571646228025	27.257561188640008	28.272904369861777	21.971962795270194
50-51	22.466139710468138	27.430378502141377	28.143863064262593	21.959618723127893
52-53	22.684127620308146	27.334532608796962	28.04987246047207	21.93146731042282
54-55	22.637422065269575	27.258018177937483	28.078660483422226	22.025899273370715
56-57	22.592688226758312	27.401014221361347	28.079269393115403	21.927028158764944
58-59	22.596537242842317	27.37088082929246	28.072578868816034	21.960003059049193
60-61	22.639083612016115	27.359608258962353	28.128749649241875	21.87255847977966
62-63	22.637472988761257	27.38160289084875	28.13932629576402	21.84159782462598
64-65	22.645048665378024	27.278698012620932	28.13345151838532	21.942801803615723
66-67	22.758130708568352	27.309055570512104	27.988101882555338	21.944711838364206
68-69	22.639533581928884	27.29524817710214	28.17825480094359	21.886963440025383
70-71	22.766498086356762	27.076474061523253	28.192048379125996	21.96497947299399
72-73	22.881323112874345	27.15963689636612	27.99139967995734	21.967640310802203
74-75	22.562726570845825	27.231120502749068	28.12799803116809	22.078154895237017
76-77	22.55304138849822	27.31166720305222	28.02367953346407	22.111611874985492
78-79	22.588189263821672	27.233949395235392	27.948708385735543	22.229152955207393
80-81	22.528290219692913	27.363441421524904	27.947183188326736	22.16108517045545
82-83	22.567267813440875	27.312940819712683	27.994589693175975	22.125201673670468
84-85	22.6230644184772	27.325620820102536	27.93483103817136	22.116483723248905
86-87	22.4849395919145	27.345227744672094	27.996888262659915	22.17294440075349
88-89	22.560464251812828	27.361742248376235	27.950338492689447	22.127455007121487
90-91	22.592912095064325	27.41052235222132	27.907583215481047	22.088982337233308
92-93	22.579676894793227	27.398067363091506	27.902783588255986	22.11947215385928
94-95	22.455171323235305	27.466131506871555	27.887357902042126	22.191339267851014
96-97	22.65258744680094	27.532463050599098	27.902090672834966	21.912858829764996
98-99	23.62468068895689	29.499227441974725	28.113066877770304	18.76302499129809
100	22.621859378293273	30.27842568557826	22.09311526285606	25.006599673272405
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	578.0
1	565.5
2	786.5
3	1125.5
4	1461.5
5	2126.0
6	2725.5
7	3503.0
8	4653.5
9	5346.0
10	5372.0
11	5299.0
12	5673.5
13	6908.5
14	9565.5
15	12822.0
16	15777.5
17	18106.0
18	18682.0
19	17489.0
20	15549.5
21	13696.5
22	13106.5
23	14303.5
24	17336.5
25	22637.0
26	29540.0
27	37118.0
28	45316.5
29	55065.0
30	64747.0
31	74060.5
32	85087.5
33	96453.5
34	106961.0
35	117131.0
36	128510.0
37	138425.0
38	146162.5
39	152993.0
40	159709.0
41	165016.5
42	167420.5
43	171637.0
44	175785.0
45	178283.0
46	179471.0
47	178737.0
48	178180.0
49	176786.0
50	175287.5
51	171833.0
52	165812.0
53	157841.5
54	147570.0
55	137734.5
56	127049.5
57	114884.0
58	102809.5
59	89833.0
60	75873.0
61	61885.5
62	49005.5
63	37926.5
64	28558.0
65	21460.5
66	16150.0
67	12362.5
68	9329.5
69	6795.5
70	4962.0
71	3581.5
72	2500.5
73	1655.0
74	1065.0
75	680.5
76	417.0
77	241.5
78	156.5
79	111.0
80	70.5
81	42.5
82	26.5
83	17.0
84	14.0
85	12.0
86	9.0
87	8.0
88	5.0
89	2.0
90	2.0
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0020708876475972818
3	0.0016287880374360644
4	0.0
5	0.0
6	4.6536801069601836E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.5598632753753137E-4
26-27	0.002013212258205062
28-29	0.006914370296423943
30-31	0.0026795205988146037
32-33	0.005625238226504769
34-35	0.002713785786149235
36-37	0.0011854689674154488
38-39	1.1800777388011213E-5
40-41	5.9363640767339166E-5
42-43	0.002389400047931365
44-45	4.693458774643818E-4
46-47	2.0632665490361994E-4
48-49	0.0016523303549364715
50-51	5.305148436819507E-4
52-53	0.0
54-55	0.00489444644311439
56-57	0.04268901431520084
58-59	0.06420641682668464
60-61	0.030911021259885024
62-63	0.012922367584499361
64-65	0.0015107176299445073
66-67	0.0
68-69	0.0020270571052458646
70-71	0.0033025032841189034
72-73	0.011562730714930252
74-75	0.015093369982049914
76-77	0.0198443870460236
78-79	0.0053930179134150285
80-81	0.007392843671319771
82-83	0.030569663271341394
84-85	0.017456391137348825
86-87	0.01285343154200399
88-89	0.005646805145345562
90-91	1.4708551522438042E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27.0
20-21	147.0
22-23	318.0
24-25	418.0
26-27	897.0
28-29	2347.0
30-31	7238.0
32-33	9042.0
34-35	12542.0
36-37	21110.0
38-39	25954.0
40-41	25628.0
42-43	29123.0
44-45	34266.0
46-47	35014.0
48-49	33210.0
50-51	30064.0
52-53	28538.0
54-55	30297.0
56-57	31776.0
58-59	31834.0
60-61	31215.0
62-63	29774.0
64-65	31308.0
66-67	33225.0
68-69	34186.0
70-71	34247.0
72-73	32147.0
74-75	32577.0
76-77	33930.0
78-79	35412.0
80-81	35193.0
82-83	33502.0
84-85	32940.0
86-87	33908.0
88-89	35580.0
90-91	36656.0
92-93	36050.0
94-95	42365.0
96-97	134163.0
98-99	303304.0
100-101	2856202.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.30196267288673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.42060644848122	82.53341547267824
2	5.6255221652334315	10.047403408303456
3	1.15486385453099	3.0939482648877767
4	0.37705481153378395	1.3468693882088925
5	0.1761659000620226	0.7865980315787117
6	0.08393154814173408	0.4497151187538439
7	0.04721969705596418	0.29517681367417126
8	0.027987067410842704	0.19994400393973116
9	0.019517524646324377	0.15686579316898855
>10	0.06481085302998228	0.8876980105735096
>50	0.0016202958399167383	0.09271400437474271
>100	6.635331097408728E-4	0.08903419952980234
>500	3.630092402515166E-5	0.020617490328135087
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.6536801069601836E-5	0.0	0.0	0.0
3	0.0	4.6536801069601836E-5	0.0	0.0	0.0
4	0.0	6.980520160440275E-5	0.0	0.0	0.0
5	0.0	6.980520160440275E-5	0.0	0.0	0.0
6	6.980520160440275E-5	6.980520160440275E-5	0.0	0.0	0.0
7	6.980520160440275E-5	6.980520160440275E-5	0.0	2.3268400534800918E-5	0.0
8	6.980520160440275E-5	6.980520160440275E-5	0.0	2.3268400534800918E-5	2.3268400534800918E-5
9	6.980520160440275E-5	9.307360213920367E-5	0.0	4.6536801069601836E-5	4.6536801069601836E-5
10-11	6.980520160440275E-5	9.307360213920367E-5	0.0	8.143940187180321E-5	4.6536801069601836E-5
12-13	6.980520160440275E-5	9.307360213920367E-5	0.0	1.1634200267400459E-4	4.6536801069601836E-5
14-15	9.307360213920367E-5	1.2797620294140505E-4	0.0	1.1634200267400459E-4	4.6536801069601836E-5
16-17	1.396104032088055E-4	1.396104032088055E-4	0.0	1.1634200267400459E-4	5.8171001337002296E-5
18-19	1.396104032088055E-4	1.6287880374360643E-4	0.0	1.396104032088055E-4	6.980520160440275E-5
20-21	1.396104032088055E-4	1.977814045458078E-4	0.0	1.6287880374360643E-4	6.980520160440275E-5
22-23	1.396104032088055E-4	2.6758660615021053E-4	0.0	1.745130040110069E-4	8.143940187180321E-5
24-25	1.396104032088055E-4	3.0248920695241194E-4	0.0	2.0941560481320826E-4	9.307360213920367E-5
26-27	1.396104032088055E-4	3.2575760748721286E-4	0.0	2.559524058828101E-4	9.307360213920367E-5
28-29	1.396104032088055E-4	3.2575760748721286E-4	0.0	5.58441612835222E-4	9.307360213920367E-5
30-31	1.396104032088055E-4	4.1883120962641653E-4	0.0	0.0017102274393078676	1.0470780240660413E-4
32-33	1.5124460347620597E-4	4.1883120962641653E-4	0.0	0.004211580496798966	1.1634200267400459E-4
34-35	1.6287880374360643E-4	4.6536801069601836E-4	0.0	0.006631494152418262	1.1634200267400459E-4
36-37	1.6287880374360643E-4	5.119048117656202E-4	0.0	0.010668561645206222	1.1634200267400459E-4
38-39	1.6287880374360643E-4	5.351732123004211E-4	0.0	0.017532739802972493	1.1634200267400459E-4
40-41	1.6287880374360643E-4	6.282468144396247E-4	0.0	0.02515314097811979	1.1634200267400459E-4
42-43	1.6287880374360643E-4	6.282468144396247E-4	0.0	0.03241288194497768	1.1634200267400459E-4
44-45	1.6287880374360643E-4	6.515152149744257E-4	0.0	0.0406498957342972	1.1634200267400459E-4
46-47	1.6287880374360643E-4	6.747836155092266E-4	0.0	0.04917776453030174	1.1634200267400459E-4
48-49	1.6287880374360643E-4	6.747836155092266E-4	0.0	0.05772890172684108	1.1634200267400459E-4
50-51	1.8614720427840735E-4	7.911256181832312E-4	0.0	0.06695482253888965	1.1634200267400459E-4
52-53	1.8614720427840735E-4	7.911256181832312E-4	0.0	0.07555249653649858	1.1634200267400459E-4
54-55	1.8614720427840735E-4	7.911256181832312E-4	0.0	0.0837080709239463	1.1634200267400459E-4
56-57	1.8614720427840735E-4	7.911256181832312E-4	0.0	0.08953680525791394	1.1634200267400459E-4
58-59	1.8614720427840735E-4	8.027598184506316E-4	0.0	0.09503978198439435	1.1634200267400459E-4
60-61	1.977814045458078E-4	8.376624192528331E-4	0.0	0.10058929551194437	1.396104032088055E-4
62-63	2.559524058828101E-4	8.376624192528331E-4	0.0	0.10562690422772877	1.6287880374360643E-4
64-65	2.559524058828101E-4	8.841992203224349E-4	0.0	0.11108334415313958	1.6287880374360643E-4
66-67	2.559524058828101E-4	8.841992203224349E-4	0.0	0.11642344207587639	1.6287880374360643E-4
68-69	2.559524058828101E-4	9.191018211246362E-4	0.0	0.1216471979959392	1.6287880374360643E-4
70-71	2.559524058828101E-4	9.307360213920367E-4	0.0	0.12582387589193594	1.6287880374360643E-4
72-73	3.0248920695241194E-4	9.307360213920367E-4	0.0	0.1294304779748301	1.6287880374360643E-4
74-75	3.0248920695241194E-4	0.00101217542326384	0.0	0.13278112765184144	1.6287880374360643E-4
76-77	3.0248920695241194E-4	0.0010587122243334419	0.0	0.13542209111254133	1.6287880374360643E-4
78-79	3.0248920695241194E-4	0.0010703464246008421	0.0	0.1372951973555928	1.6287880374360643E-4
80-81	3.0248920695241194E-4	0.0010703464246008421	0.0	0.13779546796709102	1.6287880374360643E-4
82-83	3.0248920695241194E-4	0.0010819806248682426	0.0	0.13831900697912405	1.8614720427840735E-4
84-85	3.0248920695241194E-4	0.001116883225670444	0.0	0.13859822778554165	1.8614720427840735E-4
86-87	3.0248920695241194E-4	0.001116883225670444	0.0	0.13869130138768088	1.8614720427840735E-4
88	3.0248920695241194E-4	0.001140151626205245	0.0	0.13870293558794827	2.0941560481320826E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6180	0.0	50.716198	1
GTATCAA	11700	0.0	40.201157	1
TCAACGC	13540	0.0	34.039036	4
ATCAACG	13585	0.0	33.959423	3
TATCAAC	13830	0.0	33.575626	2
CAACGCA	13870	0.0	33.22917	5
AACGCAG	14250	0.0	32.343056	6
ACGCAGA	16290	0.0	28.319048	7
CGCAGAG	16885	0.0	27.371914	8
TGGTATC	2630	0.0	25.595387	2
GTGGTAT	2885	0.0	25.265093	1
GCAGAGT	19290	0.0	24.003744	9
GTACATG	14850	0.0	21.279373	1
GAGTACT	11100	0.0	21.146994	12-13
TACATGG	14885	0.0	20.538015	2
ACATGGG	14785	0.0	20.212458	3
CAGAGTA	19050	0.0	20.153852	10-11
GTACTTT	12015	0.0	19.48325	14-15
AGAGTAC	17650	0.0	18.849703	10-11
GTCAACG	565	0.0	18.212961	1
>>END_MODULE