FastQCFastQC Report
Fri 27 May 2016
SRR2139443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4723522
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76900.047.421171
GTATCAA135800.036.01291
TCAACGC157600.030.3028374
ATCAACG158400.030.2037123
TATCAAC160900.029.999212
CAACGCA160450.029.8174975
AACGCAG162250.029.4605376
ACGCAGA185650.025.792967
CGCAGAG190800.025.0967648
GTGGTAT42650.022.7940671
TGGTATC39900.022.3435332
GCAGAGT218550.021.9295719
GTACATG182300.021.5640181
TACATGG182050.021.1039282
ACATGGG182350.020.4632553
GAGTACT112400.020.33791512-13
CAGAGTA214800.019.35785310-11
GTACTTT120450.018.78513314-15
CATGGGG129950.018.5549184
AGAGTAC198800.017.92632910-11
Produced by FastQC (version 0.11.5)