##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4723522
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.84327542033254	28.0	18.0	33.0	18.0	33.0
2	30.38787857873849	32.0	29.0	33.0	27.0	34.0
3	32.10095115466806	33.0	31.0	33.0	29.0	34.0
4	32.05921619503413	33.0	33.0	33.0	30.0	34.0
5	32.570666549240165	33.0	33.0	33.0	32.0	34.0
6	35.963976879963724	38.0	36.0	38.0	33.0	38.0
7	37.00768579039115	38.0	38.0	38.0	35.0	38.0
8	36.55898056577274	38.0	37.0	38.0	34.0	38.0
9	37.15607612285917	38.0	38.0	38.0	36.0	38.0
10-11	37.33184803627462	38.0	38.0	38.0	36.5	38.0
12-13	37.3294549067412	38.0	38.0	38.0	36.0	38.0
14-15	37.3362246433911	38.0	38.0	38.0	36.5	38.0
16-17	37.37661463204786	38.0	38.0	38.0	37.0	38.0
18-19	37.39728024977972	38.0	38.0	38.0	37.0	38.0
20-21	37.42335005360077	38.0	38.0	38.0	37.0	38.0
22-23	37.43999226454981	38.0	38.0	38.0	37.0	38.0
24-25	37.45208708850632	38.0	38.0	38.0	37.0	38.0
26-27	37.42516153746001	38.0	38.0	38.0	37.0	38.0
28-29	37.401127747589854	38.0	38.0	38.0	37.0	38.0
30-31	37.41483850582862	38.0	38.0	38.0	37.0	38.0
32-33	37.40346692973613	38.0	38.0	38.0	37.0	38.0
34-35	37.379995626918756	38.0	38.0	38.0	37.0	38.0
36-37	37.362738368503315	38.0	38.0	38.0	37.0	38.0
38-39	37.354547293097184	38.0	38.0	38.0	37.0	38.0
40-41	37.34918465498063	38.0	38.0	38.0	37.0	38.0
42-43	37.315659430533614	38.0	38.0	38.0	37.0	38.0
44-45	37.27918988674056	38.0	38.0	38.0	37.0	38.0
46-47	37.251356245903196	38.0	38.0	38.0	37.0	38.0
48-49	37.19767402074207	38.0	38.0	38.0	37.0	38.0
50-51	37.14875187263499	38.0	38.0	38.0	36.0	38.0
52-53	37.129518809522395	38.0	38.0	38.0	36.0	38.0
54-55	37.10407790583986	38.0	38.0	38.0	36.0	38.0
56-57	37.04376913291658	38.0	38.0	38.0	36.0	38.0
58-59	37.01457291423493	38.0	38.0	38.0	36.0	38.0
60-61	37.037352241088456	38.0	38.0	38.0	36.0	38.0
62-63	37.04268440250383	38.0	38.0	38.0	36.0	38.0
64-65	37.047920689811235	38.0	38.0	38.0	36.0	38.0
66-67	37.04062618861485	38.0	38.0	38.0	36.0	38.0
68-69	37.02770992715791	38.0	38.0	38.0	36.0	38.0
70-71	37.01467702128218	38.0	38.0	38.0	36.0	38.0
72-73	37.007778564151366	38.0	38.0	38.0	36.0	38.0
74-75	36.98632369787144	38.0	38.0	38.0	36.0	38.0
76-77	36.963520428875654	38.0	38.0	38.0	36.0	38.0
78-79	36.96604785658624	38.0	38.0	38.0	36.0	38.0
80-81	36.960898426496215	38.0	38.0	38.0	35.5	38.0
82-83	36.908542651556225	38.0	38.0	38.0	35.0	38.0
84-85	36.885081487295494	38.0	38.0	38.0	35.0	38.0
86-87	36.883534569033635	38.0	38.0	38.0	35.0	38.0
88-89	36.86550864995897	38.0	38.0	38.0	35.0	38.0
90-91	36.835939124065206	38.0	38.0	38.0	35.0	38.0
92-93	36.8205881794299	38.0	38.0	38.0	35.0	38.0
94-95	36.79760865219347	38.0	38.0	38.0	35.0	38.0
96-97	36.76303707909432	38.0	38.0	38.0	35.0	38.0
98-99	36.74046713953332	38.0	38.0	38.0	34.5	38.0
100	35.29047847492018	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	11.0
21	66.0
22	244.0
23	784.0
24	1804.0
25	3736.0
26	6772.0
27	11188.0
28	17910.0
29	26893.0
30	36877.0
31	48171.0
32	63866.0
33	86784.0
34	134246.0
35	254815.0
36	821549.0
37	3207802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.025736304393206	17.44787046614793	13.225660852220017	22.300732377238848
2	14.839102234590035	20.059144187669318	42.92770080357846	22.17405277416219
3	18.943910417906935	26.011380679013023	29.838948021824752	25.205760881255284
4	13.125544879435303	18.032540125779025	38.204056210598786	30.637858784186882
5	14.244017173633223	37.21680018291444	34.875707099789985	13.663475543662354
6	30.664196470761585	37.0621266549127	18.637594230733402	13.63608264359231
7	27.626885192870915	32.63088432741501	22.27009845619434	17.472132023519737
8	24.939017961597298	36.61333640448801	20.53313184526292	17.914513788651774
9	26.04228370271166	17.90286993476478	21.208729418429723	34.846116944093836
10-11	24.93622343666442	26.859100052884266	27.989972313032517	20.21470419741879
12-13	25.82957166283972	24.32658300310658	28.361188960271594	21.482656373782106
14-15	22.92716113103739	25.610169699643613	26.333051904913322	25.129617264405667
16-17	22.231155057603203	28.852443155763858	26.974395377008936	21.942006409624003
18-19	22.209052905861345	27.89912908207054	28.84052408351226	21.051293928555854
20-21	23.177913084347605	26.89031064290145	28.7946894523683	21.137086820382645
22-23	22.954134667683775	26.687474606712357	28.68762408173955	21.670766643864315
24-25	22.62142148047071	26.890432200876784	28.7413831855172	21.746763133135307
26-27	22.453667232511336	27.016668699641517	28.77757433911376	21.752089728733388
28-29	22.438074884255187	27.03022139703892	28.800543948708324	21.73115976999757
30-31	22.69841929534321	26.924534319140932	28.68225346999723	21.694792915518622
32-33	22.453987286842228	27.152762409544035	28.58847741391112	21.80477288970261
34-35	22.64537573821443	27.101043564278598	28.62381671839765	21.629763979109327
36-37	22.594231400242222	26.987707469079368	28.58854074644163	21.82952038423678
38-39	22.428213357333053	27.199513377282603	28.682074951347413	21.69019831403693
40-41	22.70155013190111	27.038877708013302	28.52271512316963	21.736857036915954
42-43	22.591999560248805	27.122625689824243	28.368260215790013	21.917114534136942
44-45	22.641357375598606	27.227729086048246	28.2510470224454	21.879866515907747
46-47	22.708522145998657	27.256416541925926	27.970515428475423	22.064545883599994
48-49	22.6579247168808	27.34712638322864	27.920667040888596	22.074281859001957
50-51	22.6538747962013	27.49202654534544	27.831954641910773	22.022144016542487
52-53	22.86215718589962	27.41347879354899	27.689462086516137	22.034901934035254
54-55	22.807999956836664	27.330151414913644	27.730055123941337	22.131793504308355
56-57	22.760408769783737	27.515964999721433	27.702530759257893	22.02109547123694
58-59	22.792117663491158	27.440180887689152	27.719226680583503	22.048474768236186
60-61	22.827632858989357	27.469957979131504	27.736484686267605	21.965924475611533
62-63	22.828322504528316	27.51224630613554	27.720056646150347	21.939374543185792
64-65	22.89448382022903	27.447298256382712	27.65934005332515	21.998877870063108
66-67	23.05524895767812	27.48079624547559	27.477493336411207	21.98646146043508
68-69	22.831348131510794	27.527256360428304	27.667267517338296	21.97412799072261
70-71	22.943675926890425	27.287772071423177	27.706089058296808	22.062462943389594
72-73	23.080387937402314	27.374126859714476	27.496465300477198	22.049019902406013
74-75	22.69921198553314	27.41988511195656	27.690049992586324	22.19085290992398
76-77	22.721276342300627	27.382262856207195	27.689225528829205	22.20723527266297
78-79	22.723811302380703	27.32003127227209	27.622568798549054	22.33358862679816
80-81	22.72598604490271	27.431702191534075	27.526011287486014	22.3163004760772
82-83	22.717615417976354	27.321361771209613	27.71433744752654	22.24668536328749
84-85	22.7760367680735	27.287052082754425	27.6782918247646	22.258619324407476
86-87	22.619780536696354	27.362034043855672	27.723276613228958	22.29490880621901
88-89	22.694931120295976	27.359134942987467	27.660311770578904	22.285622166137657
90-91	22.709885432952724	27.411149219434055	27.642804583778098	22.236160763835127
92-93	22.773909631054625	27.384496556649395	27.605371130692347	22.236222681603632
94-95	22.610692006251014	27.43361242953687	27.642966143050934	22.31272942116118
96-97	22.784555616333968	27.4977170069719	27.648328951773948	22.069398424920188
98-99	23.853742924484273	29.455436188409706	27.78161165857695	18.909209228529072
100	30.46834721850844	40.43325416424178	29.098398617249778	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	575.0
1	562.5
2	709.0
3	990.5
4	1367.0
5	2043.0
6	2618.0
7	3186.0
8	3951.0
9	4516.0
10	4813.5
11	5068.5
12	5637.0
13	7032.0
14	9846.5
15	13112.5
16	16003.5
17	18506.0
18	19223.5
19	17847.5
20	15891.5
21	14272.5
22	13922.0
23	15556.0
24	19225.5
25	24939.0
26	32757.0
27	41695.0
28	50801.5
29	61340.5
30	71728.5
31	81822.0
32	93555.5
33	106453.5
34	118626.5
35	129752.0
36	142618.0
37	153287.0
38	161283.5
39	169357.0
40	177498.5
41	183531.5
42	187188.0
43	191967.0
44	196654.0
45	199680.5
46	200528.0
47	200923.0
48	201329.0
49	200443.5
50	197870.5
51	193399.5
52	186627.5
53	177758.5
54	167075.5
55	155951.5
56	143353.5
57	129232.0
58	115264.0
59	99798.5
60	84013.0
61	68619.0
62	54011.0
63	41527.5
64	31553.5
65	23873.0
66	17748.0
67	13439.5
68	10283.5
69	7662.5
70	5564.0
71	3889.0
72	2670.0
73	1759.5
74	1134.5
75	735.5
76	478.0
77	296.5
78	201.5
79	140.5
80	86.5
81	57.0
82	43.5
83	29.0
84	21.5
85	17.5
86	10.5
87	5.5
88	4.5
89	3.0
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00317559651463463
3	0.001651310187610008
4	0.0
5	4.2341286861795074E-5
6	2.1170643430897538E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.646719296150527E-4
26-27	0.0020858733959554176
28-29	0.008473323224658727
30-31	0.002968530607034824
32-33	0.005864227204808666
34-35	0.003174385056102995
36-37	0.0012513121398132765
38-39	6.456161373044752E-5
40-41	6.499455183169271E-5
42-43	0.0026720278815748517
44-45	7.366374785976578E-4
46-47	2.774269251930503E-4
48-49	0.0022511048052799945
50-51	6.100367309893914E-4
52-53	0.0
54-55	0.005085208814974161
56-57	0.04771035450147947
58-59	0.07044230902203509
60-61	0.03524860163458761
62-63	0.01510795411793975
64-65	0.0016064422650644125
66-67	0.0
68-69	0.002223513434663646
70-71	0.004924183008310516
72-73	0.014708868487882198
74-75	0.01716556286056806
76-77	0.0226617416673297
78-79	0.006331630514297926
80-81	0.008166814920108685
82-83	0.033589059968350946
84-85	0.01973251043813997
86-87	0.014873112253675802
88-89	0.0068678555507105795
90-91	6.851271904372687E-5
92-93	0.0
94-95	0.0
96-97	2.8519579975033957E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	208.0
22-23	358.0
24-25	482.0
26-27	1152.0
28-29	3024.0
30-31	9033.0
32-33	11857.0
34-35	16170.0
36-37	26603.0
38-39	31278.0
40-41	30556.0
42-43	35071.0
44-45	41253.0
46-47	41933.0
48-49	39385.0
50-51	35836.0
52-53	34329.0
54-55	36795.0
56-57	37833.0
58-59	38124.0
60-61	36995.0
62-63	35349.0
64-65	37329.0
66-67	39726.0
68-69	40971.0
70-71	40958.0
72-73	38276.0
74-75	38589.0
76-77	40376.0
78-79	41967.0
80-81	42463.0
82-83	39149.0
84-85	38572.0
86-87	40097.0
88-89	41785.0
90-91	42209.0
92-93	41631.0
94-95	48266.0
96-97	145024.0
98-99	1093695.0
100-101	2288772.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.75060241393798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.30319268940356	81.91963955914362
2	5.587928009558815	9.918639541881245
3	1.2140044674895187	3.232308834687203
4	0.41054687358973413	1.45745129400991
5	0.19026488375717887	0.8443061525833755
6	0.09405800850582019	0.5008622950048107
7	0.05841430621880201	0.3629013406557646
8	0.036352215180210895	0.2581024797059855
9	0.022707867372299607	0.18138032179446573
>10	0.08011718904704658	1.0896445160304984
>50	0.0016811135559042355	0.10454974690234259
>100	6.9794876223209E-4	0.10772568351661777
>500	3.4427558849397796E-5	0.022488234084186622
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.1170643430897537E-5	0.0
3	0.0	0.0	0.0	4.2341286861795074E-5	0.0
4	0.0	2.1170643430897537E-5	0.0	6.351193029269261E-5	0.0
5	0.0	2.1170643430897537E-5	0.0	6.351193029269261E-5	0.0
6	4.2341286861795074E-5	2.1170643430897537E-5	0.0	6.351193029269261E-5	2.1170643430897537E-5
7	4.2341286861795074E-5	4.2341286861795074E-5	0.0	6.351193029269261E-5	4.2341286861795074E-5
8	4.2341286861795074E-5	4.2341286861795074E-5	0.0	6.351193029269261E-5	6.351193029269261E-5
9	4.2341286861795074E-5	1.2702386058538521E-4	0.0	6.351193029269261E-5	8.468257372359015E-5
10-11	8.468257372359015E-5	1.2702386058538521E-4	0.0	6.351193029269261E-5	8.468257372359015E-5
12-13	8.468257372359015E-5	1.2702386058538521E-4	0.0	6.351193029269261E-5	8.468257372359015E-5
14-15	8.468257372359015E-5	1.5877982573173153E-4	0.0	6.351193029269261E-5	1.0585321715448769E-4
16-17	1.2702386058538521E-4	1.693651474471803E-4	0.0	8.468257372359015E-5	1.0585321715448769E-4
18-19	1.2702386058538521E-4	1.693651474471803E-4	0.0	9.526789543903892E-5	1.693651474471803E-4
20-21	1.2702386058538521E-4	1.693651474471803E-4	0.0	1.0585321715448769E-4	2.2229175602442417E-4
22-23	1.2702386058538521E-4	2.011211125935266E-4	0.0	1.1643853886993645E-4	2.3287707773987293E-4
24-25	1.2702386058538521E-4	2.328770777398729E-4	0.0	1.2702386058538521E-4	2.3287707773987293E-4
26-27	1.2702386058538521E-4	2.5404772117077043E-4	0.0	1.9053579087807785E-4	2.3287707773987293E-4
28-29	1.2702386058538521E-4	2.5404772117077043E-4	0.0	4.4458351204884833E-4	2.3287707773987293E-4
30-31	1.2702386058538521E-4	2.9638900803256554E-4	0.0	0.001429018431585584	2.434623994553217E-4
32-33	1.2702386058538521E-4	3.387302948943606E-4	0.0	0.0034402295575208497	2.5404772117077043E-4
34-35	1.4819450401628277E-4	4.763394771951946E-4	0.0	0.005874853552074066	2.9638900803256554E-4
36-37	1.4819450401628277E-4	5.927780160651311E-4	0.0	0.009876105160513703	2.9638900803256554E-4
38-39	1.4819450401628277E-4	6.139486594960285E-4	0.0	0.017063538605303415	3.2814497317891185E-4
40-41	1.4819450401628277E-4	6.562899463578237E-4	0.0	0.023838144503190627	3.387302948943606E-4
42-43	1.4819450401628277E-4	7.092165549350676E-4	0.0	0.03087738344396406	3.387302948943606E-4
44-45	1.4819450401628277E-4	8.891670240976966E-4	0.0	0.03798013431503018	3.5990093832525817E-4
46-47	1.4819450401628277E-4	0.0010161908846830817	0.0	0.04617317332278753	3.704862600407069E-4
48-49	1.4819450401628277E-4	0.0010161908846830817	0.0	0.05464143069514654	4.022422251870532E-4
50-51	1.4819450401628277E-4	0.001227897318992057	0.0	0.06258042198173312	4.022422251870532E-4
52-53	1.4819450401628277E-4	0.0012702386058538523	0.0	0.06993722057397	4.2341286861795075E-4
54-55	1.4819450401628277E-4	0.0013231652144310962	0.0	0.07750572560051588	4.445835120488483E-4
56-57	1.4819450401628277E-4	0.001333750536146545	0.0	0.08495779208819182	4.445835120488483E-4
58-59	1.4819450401628277E-4	0.001333750536146545	0.0	0.09169005669921723	4.5516883376429707E-4
60-61	1.5877982573173153E-4	0.0013866771447237887	0.0	0.09877163692685247	4.6575415547974586E-4
62-63	1.693651474471803E-4	0.0013972624664392375	0.0	0.10529219510356891	4.6575415547974586E-4
64-65	1.693651474471803E-4	0.0015666276138864177	0.0	0.11136816976823649	4.6575415547974586E-4
66-67	1.693651474471803E-4	0.0015666276138864177	0.0	0.1174441444329041	4.6575415547974586E-4
68-69	1.693651474471803E-4	0.0015877982573173153	0.0	0.12400704389648232	4.6575415547974586E-4
70-71	1.693651474471803E-4	0.001608968900748213	0.0	0.12927853411077583	4.6575415547974586E-4
72-73	1.693651474471803E-4	0.001608968900748213	0.0	0.13401017291758144	4.869247989106434E-4
74-75	1.693651474471803E-4	0.001608968900748213	0.0	0.1374821584402486	4.975101206260921E-4
76-77	1.693651474471803E-4	0.0016618955093254568	0.0	0.14079536413718408	5.080954423415409E-4
78-79	1.693651474471803E-4	0.001693651474471803	0.0	0.14338876795746902	5.080954423415409E-4
80-81	1.693651474471803E-4	0.001693651474471803	0.0	0.14417208176441224	5.292660857724384E-4
82-83	1.693651474471803E-4	0.001693651474471803	0.0	0.14474368913704647	5.50436729203336E-4
84-85	1.693651474471803E-4	0.001693651474471803	0.0	0.14491305428449364	5.50436729203336E-4
86-87	1.693651474471803E-4	0.0017571634047644957	0.0	0.14509300475365627	5.50436729203336E-4
88	1.693651474471803E-4	0.0018418459784880859	0.0	0.1451035900753717	5.50436729203336E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7690	0.0	47.42117	1
GTATCAA	13580	0.0	36.0129	1
TCAACGC	15760	0.0	30.302837	4
ATCAACG	15840	0.0	30.203712	3
TATCAAC	16090	0.0	29.99921	2
CAACGCA	16045	0.0	29.817497	5
AACGCAG	16225	0.0	29.460537	6
ACGCAGA	18565	0.0	25.79296	7
CGCAGAG	19080	0.0	25.096764	8
GTGGTAT	4265	0.0	22.794067	1
TGGTATC	3990	0.0	22.343533	2
GCAGAGT	21855	0.0	21.929571	9
GTACATG	18230	0.0	21.564018	1
TACATGG	18205	0.0	21.103928	2
ACATGGG	18235	0.0	20.463255	3
GAGTACT	11240	0.0	20.337915	12-13
CAGAGTA	21480	0.0	19.357853	10-11
GTACTTT	12045	0.0	18.785133	14-15
CATGGGG	12995	0.0	18.554918	4
AGAGTAC	19880	0.0	17.926329	10-11
>>END_MODULE