FastQCFastQC Report
Fri 27 May 2016
SRR2139442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439231
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103200.041.877481
CTTATAC48100.036.5428921
GTATCAA175050.035.2417071
ATCAACG200000.030.0549373
TCAACGC201150.029.9255264
TATCAAC206300.029.4690672
CAACGCA205650.029.229215
TATACAC74150.028.8244723
AACGCAG212600.028.3539586
ACGCAGA236800.025.4382847
TTATACA75350.025.4222432
CGCAGAG242650.024.8074158
ACATCTC82250.022.9774938
ACACATC81800.022.843136
TACACAT92700.022.3661565
GCAGAGT273250.022.154259
GTACATG189700.021.2527561
TACATGG186000.020.9186042
GAGTACT154400.020.90241212-13
TATGCCG52850.020.747442-43
Produced by FastQC (version 0.11.5)