##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5439231
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.690146640214397	28.0	18.0	33.0	18.0	33.0
2	30.236320906392834	31.0	28.0	33.0	27.0	34.0
3	32.00341757869816	33.0	31.0	33.0	29.0	34.0
4	31.937011868037963	33.0	31.0	33.0	29.0	34.0
5	32.521619140646905	33.0	33.0	33.0	32.0	34.0
6	35.897504077322694	38.0	36.0	38.0	31.0	38.0
7	36.94391560130467	38.0	37.0	38.0	35.0	38.0
8	36.46677811624474	38.0	37.0	38.0	34.0	38.0
9	37.102149917883615	38.0	38.0	38.0	36.0	38.0
10-11	37.26934616308813	38.0	38.0	38.0	36.0	38.0
12-13	37.268190117316216	38.0	38.0	38.0	36.0	38.0
14-15	37.26752954599648	38.0	38.0	38.0	36.5	38.0
16-17	37.31058011693197	38.0	38.0	38.0	36.5	38.0
18-19	37.33611120763211	38.0	38.0	38.0	37.0	38.0
20-21	37.36676926253968	38.0	38.0	38.0	37.0	38.0
22-23	37.38245433430666	38.0	38.0	38.0	37.0	38.0
24-25	37.39352790054477	38.0	38.0	38.0	37.0	38.0
26-27	37.366081490463756	38.0	38.0	38.0	37.0	38.0
28-29	37.339842636483084	38.0	38.0	38.0	37.0	38.0
30-31	37.34622416110379	38.0	38.0	38.0	37.0	38.0
32-33	37.33052610461597	38.0	38.0	38.0	37.0	38.0
34-35	37.29926413919424	38.0	38.0	38.0	37.0	38.0
36-37	37.27235625920106	38.0	38.0	38.0	37.0	38.0
38-39	37.24178058363462	38.0	38.0	38.0	37.0	38.0
40-41	37.262180093489064	38.0	38.0	38.0	37.0	38.0
42-43	37.22800404832443	38.0	38.0	38.0	37.0	38.0
44-45	37.18289754773623	38.0	38.0	38.0	36.0	38.0
46-47	37.148629355544045	38.0	38.0	38.0	36.0	38.0
48-49	37.088853912382206	38.0	38.0	38.0	36.0	38.0
50-51	37.0283028389076	38.0	38.0	38.0	36.0	38.0
52-53	37.00534109249807	38.0	38.0	38.0	36.0	38.0
54-55	36.97196399857661	38.0	38.0	38.0	36.0	38.0
56-57	36.90589204946519	38.0	38.0	38.0	36.0	38.0
58-59	36.87062260703368	38.0	38.0	38.0	35.5	38.0
60-61	36.88756811493584	38.0	38.0	38.0	35.5	38.0
62-63	36.89211029732883	38.0	38.0	38.0	35.0	38.0
64-65	36.89618377089744	38.0	38.0	38.0	35.0	38.0
66-67	36.887506155216876	38.0	38.0	38.0	35.0	38.0
68-69	36.87087407709605	38.0	38.0	38.0	35.0	38.0
70-71	36.860942643527366	38.0	38.0	38.0	35.0	38.0
72-73	36.854677073388494	38.0	38.0	38.0	35.0	38.0
74-75	36.83349003303728	38.0	38.0	38.0	35.0	38.0
76-77	36.8118743635343	38.0	38.0	38.0	35.0	38.0
78-79	36.8135183907621	38.0	38.0	38.0	35.0	38.0
80-81	36.807905165656635	38.0	38.0	38.0	35.0	38.0
82-83	36.75295105608433	38.0	38.0	38.0	35.0	38.0
84-85	36.73067007062308	38.0	38.0	38.0	35.0	38.0
86-87	36.7304503545719	38.0	38.0	38.0	35.0	38.0
88-89	36.71393398418481	38.0	38.0	38.0	34.0	38.0
90-91	36.67683760854683	38.0	38.0	38.0	34.0	38.0
92-93	36.66392256404275	38.0	38.0	38.0	34.0	38.0
94-95	36.63949311024605	38.0	38.0	38.0	34.0	38.0
96-97	36.607803476839386	38.0	38.0	38.0	34.0	38.0
98-99	36.59594780155304	38.0	38.0	38.0	34.0	38.0
100	35.0208923486941	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	37.0
21	104.0
22	398.0
23	1060.0
24	2626.0
25	5285.0
26	9289.0
27	16035.0
28	25598.0
29	37572.0
30	50595.0
31	64269.0
32	83673.0
33	114035.0
34	176380.0
35	328401.0
36	1008337.0
37	3515530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.846033933841014	17.245470913075764	13.47745664782393	22.43103850525929
2	14.507726581016167	19.833589534702647	43.75525939032715	21.903424493954034
3	18.75863418063671	26.126106747061762	30.05101175958396	25.06424731271757
4	12.724537714982137	18.386808723512573	38.29133199159955	30.597321569905745
5	13.874103801882143	37.14077386363908	35.280969152418166	13.704153182060614
6	29.98065532877484	37.424603215458674	18.671395037233253	13.92334641853323
7	26.98912769102838	32.600656232471096	22.362499404787183	18.047716671713335
8	24.307075761261103	37.04126557596101	20.66876733126429	17.98289133151359
9	25.297178957834298	18.006994003380257	21.434813119722254	35.26101391906319
10-11	24.65123286729319	26.973436134630063	28.075393378218354	20.299937619858397
12-13	25.597561493527305	24.21363424351714	28.564212477830047	21.624591785125507
14-15	22.56204047961927	25.63322830010345	26.450329099830473	25.354402120446807
16-17	21.891973332259653	29.067610844253533	27.06382575036802	21.976590073118793
18-19	21.844126495087266	27.794627586142234	29.192849136210615	21.16839678255989
20-21	22.659541032348578	26.832111280796795	29.15039073721469	21.357956949639938
22-23	22.395608485025882	26.909915916079346	29.07023393907391	21.624241659820857
24-25	22.23540836033437	27.048954549073613	29.198872235822087	21.516764854769928
26-27	22.15292704266783	27.134648974189098	29.24595783653508	21.466466146607992
28-29	21.90470303052381	27.076068919287007	29.387839470926657	21.631388579262527
30-31	22.251750064817216	27.22489695535862	29.078448349517995	21.444904630306173
32-33	22.34209503328602	27.039000631533156	29.119416222044325	21.499488113136497
34-35	22.271669879813988	27.05898813231558	29.152182227621033	21.517159760249395
36-37	22.06160266953811	27.22767939926854	29.133309660369694	21.577408270823653
38-39	21.941579347933423	27.05317639451273	29.34576799634565	21.659476261208194
40-41	22.216896013045925	26.914405845866835	29.221819233908885	21.64687890717836
42-43	22.25350631855438	26.97775049309562	29.04350937153508	21.725233816814917
44-45	22.104956675983843	27.247493479913253	28.874671232712267	21.772878611390635
46-47	22.412586414375475	27.091014250688033	28.42891770882026	22.06748162611623
48-49	22.341804891228346	27.11242152286328	28.38944908851214	22.156324497396234
50-51	22.076036044176206	27.336548008068274	28.534985868202245	22.05243007955327
52-53	22.303334419287975	27.327291590051367	28.52899710693409	21.84037688372657
54-55	22.33906820900193	27.244584839605412	28.292615726011476	22.123731225381178
56-57	22.483369902082632	27.538064880077822	28.010180595671745	21.9683846221678
58-59	22.219552697593894	27.736272278052304	28.375366568914956	21.66880845543885
60-61	22.458141603186267	27.937750198938048	27.996444554795985	21.607663643079704
62-63	22.181110946178162	28.221117433616854	27.953772177853892	21.643999442351095
64-65	22.15994714761482	28.17169908688547	27.96483458649494	21.70351917900477
66-67	22.31890647936417	28.103551774007187	27.772964509830622	21.80457723679802
68-69	22.243916949529694	27.943460892277848	28.07602192756708	21.736600230625378
70-71	22.363756937228825	27.51972645133181	28.179859103641157	21.93665750779821
72-73	22.567635629743155	27.395651536368614	28.16552425548588	21.87118857840235
74-75	22.270735452919364	27.50822320889339	28.207956615743583	22.01308472244366
76-77	22.27636135979355	27.513709300531247	28.13210546371017	22.077823875965034
78-79	22.35916976044251	27.44885795718109	28.03056486793876	22.16140741443764
80-81	22.342265879223984	27.524725464407624	28.05754596081984	22.075462695548545
82-83	22.399787312062045	27.49884225035906	28.12032384551406	21.98104659206483
84-85	22.455243312255917	27.4718184216118	28.042158798660623	22.03077946747166
86-87	22.236598224362105	27.549514757915265	28.10863772983174	22.105249287890885
88-89	22.362522952145593	27.53179911198779	28.00606562399015	22.099612311876463
90-91	22.39007878733602	27.560346535430348	27.978725425195922	22.070849252037704
92-93	22.405281004487147	27.591146977852414	27.976268922003232	22.027303095657203
94-95	22.28036206483809	27.66667651565356	27.975158845063575	22.077802574444775
96-97	22.390878613705308	27.781768742951325	28.011099511177544	21.816253132165823
98-99	23.456223352197693	29.722987988717662	28.1459717268961	18.67481693218854
100	22.44054362992891	30.549359312518813	22.117102546436318	24.89299451111596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	755.0
1	740.5
2	1029.5
3	1444.5
4	1926.5
5	2938.0
6	3815.0
7	4799.0
8	6181.5
9	7043.5
10	7112.5
11	7011.0
12	7608.5
13	9410.5
14	13186.0
15	17851.5
16	22032.5
17	25393.0
18	26320.0
19	24502.0
20	21727.0
21	19322.0
22	18446.0
23	20129.5
24	24360.5
25	31329.0
26	40921.5
27	51577.5
28	62497.0
29	74506.5
30	86085.5
31	97627.0
32	110635.5
33	123751.0
34	137425.5
35	150354.0
36	163635.5
37	175695.5
38	183339.0
39	189565.5
40	197150.0
41	203406.0
42	208623.5
43	219248.5
44	230723.5
45	236626.0
46	235857.0
47	232612.5
48	230436.5
49	226635.5
50	220954.5
51	214428.5
52	206417.5
53	196231.0
54	184861.5
55	171746.5
56	158058.5
57	143702.5
58	128045.0
59	111684.5
60	94336.0
61	76844.5
62	60513.0
63	46596.5
64	34932.0
65	25518.5
66	18513.5
67	13895.0
68	10464.0
69	7550.0
70	5486.0
71	3862.5
72	2587.0
73	1713.0
74	1084.0
75	699.5
76	452.0
77	272.0
78	180.0
79	118.0
80	69.0
81	38.5
82	22.5
83	16.5
84	12.0
85	7.5
86	5.5
87	3.0
88	2.0
89	2.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0027577427765064586
3	0.0017098005214340043
4	0.0
5	7.353980737350556E-5
6	1.2869466290363473E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.4823548243049017E-4
26-27	0.0019309874288120797
28-29	0.007055211676586889
30-31	0.002531858373919023
32-33	0.0052671112625136554
34-35	0.002811116634708709
36-37	0.001122999124153493
38-39	3.733496312892379E-5
40-41	4.696831282775075E-5
42-43	0.002231301855904228
44-45	4.3825918877080875E-4
46-47	2.9798386042512875E-4
48-49	0.0016970886783141258
50-51	4.49821660379757E-4
52-53	0.0
54-55	0.004714244342360929
56-57	0.04636130301873711
58-59	0.07008979523651675
60-61	0.03311145437612883
62-63	0.01407260170095906
64-65	0.0016032543373718793
66-67	0.0
68-69	0.002206135145727893
70-71	0.0038549056236879572
72-73	0.013105763402614887
74-75	0.016050156739811913
76-77	0.021733357997485093
78-79	0.006300881423301327
80-81	0.008239739139288525
82-83	0.03343680715568082
84-85	0.0189550892583249
86-87	0.013729418584547794
88-89	0.00644393079778665
90-91	4.7197099926997885E-5
92-93	0.0
94-95	0.0
96-97	3.675171217039074E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	219.0
22-23	492.0
24-25	611.0
26-27	1499.0
28-29	3285.0
30-31	9777.0
32-33	12490.0
34-35	17016.0
36-37	28221.0
38-39	34511.0
40-41	33650.0
42-43	38353.0
44-45	45779.0
46-47	46111.0
48-49	44091.0
50-51	38664.0
52-53	36581.0
54-55	39788.0
56-57	41243.0
58-59	41214.0
60-61	40930.0
62-63	40274.0
64-65	44645.0
66-67	50015.0
68-69	51738.0
70-71	59046.0
72-73	43140.0
74-75	41301.0
76-77	43926.0
78-79	45105.0
80-81	45011.0
82-83	41846.0
84-85	41631.0
86-87	42830.0
88-89	45019.0
90-91	45694.0
92-93	45356.0
94-95	52617.0
96-97	165602.0
98-99	372289.0
100-101	3567574.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.76920372061812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.79177170028143	78.7792973481757
2	6.646998695498402	11.535095678807673
3	1.483417249902053	3.8614480067829096
4	0.49768994654820536	1.7273664144697911
5	0.22436239099390462	0.9733873005697542
6	0.11858260305664982	0.6173590825406184
7	0.06741296038114841	0.40945582249052775
8	0.04202530517374627	0.291720181283355
9	0.029222414965270505	0.22820451095969962
>10	0.09539077032700337	1.2755986908859476
>50	0.002333414067802349	0.13637229407768625
>100	7.277397645410883E-4	0.11636129861270189
>500	3.240451995863272E-5	0.01917589216543937
>1k	3.240451986411197E-5	0.02915747817823592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8384951843376388E-5	0.0	0.0	0.0
3	0.0	1.8384951843376388E-5	0.0	0.0	0.0
4	0.0	3.6769903686752775E-5	0.0	0.0	0.0
5	0.0	5.515485553012917E-5	0.0	1.8384951843376388E-5	0.0
6	0.0	5.515485553012917E-5	0.0	1.8384951843376388E-5	3.6769903686752775E-5
7	1.8384951843376388E-5	5.515485553012917E-5	0.0	3.6769903686752775E-5	5.515485553012917E-5
8	1.8384951843376388E-5	1.1030971106025834E-4	0.0	3.6769903686752775E-5	5.515485553012917E-5
9	1.8384951843376388E-5	2.0223447027714028E-4	0.0	3.6769903686752775E-5	5.515485553012917E-5
10-11	1.8384951843376388E-5	2.0223447027714028E-4	0.0	4.596237960844097E-5	5.515485553012917E-5
12-13	1.8384951843376388E-5	2.0223447027714028E-4	0.0	6.434733145181735E-5	6.434733145181735E-5
14-15	1.8384951843376388E-5	3.76891512789216E-4	0.0	7.353980737350555E-5	7.353980737350555E-5
16-17	1.8384951843376388E-5	3.860839887109042E-4	0.0	8.273228329519375E-5	7.353980737350555E-5
18-19	2.757742776506458E-5	3.9527646463259234E-4	0.0	9.192475921688194E-5	7.353980737350555E-5
20-21	5.515485553012916E-5	4.0446894055428057E-4	0.0	9.192475921688194E-5	9.192475921688194E-5
22-23	7.353980737350555E-5	4.0446894055428057E-4	0.0	1.0111723513857014E-4	1.1030971106025834E-4
24-25	9.192475921688194E-5	4.3204636831934513E-4	0.0	1.3788713882532293E-4	1.1030971106025834E-4
26-27	1.1030971106025834E-4	4.5962379608440975E-4	0.0	2.0223447027714028E-4	1.1030971106025834E-4
28-29	1.1030971106025834E-4	4.5962379608440975E-4	0.0	5.423560793796035E-4	1.1030971106025834E-4
30-31	1.1030971106025834E-4	5.147786516145389E-4	0.0	0.0013145240568014117	1.1030971106025834E-4
32-33	1.286946629036347E-4	5.147786516145389E-4	0.0	0.0031989816207474916	1.2869466290363473E-4
34-35	1.470796147470111E-4	5.883184589880444E-4	0.0	0.00565337269183824	1.470796147470111E-4
36-37	1.470796147470111E-4	6.6185826636155E-4	0.0	0.009486635151182216	1.470796147470111E-4
38-39	1.470796147470111E-4	6.6185826636155E-4	0.0	0.015553669259496425	1.470796147470111E-4
40-41	1.470796147470111E-4	7.905529292651847E-4	0.0	0.02177697545847933	1.470796147470111E-4
42-43	1.470796147470111E-4	7.905529292651847E-4	0.0	0.028138168796287562	1.470796147470111E-4
44-45	1.470796147470111E-4	8.181303570302494E-4	0.0	0.035216375255987475	1.654645665903875E-4
46-47	1.470796147470111E-4	8.457077847953139E-4	0.0	0.04236812152306089	1.838495184337639E-4
48-49	1.470796147470111E-4	8.457077847953139E-4	0.0	0.04963936997711625	2.2061942212051668E-4
50-51	1.470796147470111E-4	9.192475921688195E-4	0.0	0.05713123785329213	2.2061942212051668E-4
52-53	1.470796147470111E-4	9.192475921688195E-4	0.0	0.0638601302279679	2.2061942212051668E-4
54-55	1.470796147470111E-4	9.284400680905076E-4	0.0	0.07041436556013157	2.2061942212051668E-4
56-57	1.470796147470111E-4	9.744024476989486E-4	0.0	0.07518526056348775	2.5738932580726947E-4
58-59	1.470796147470111E-4	9.92787399542325E-4	0.0	0.08043416431477171	2.7577427765064586E-4
60-61	1.470796147470111E-4	0.001047942255072454	0.0	0.08602318967515812	2.941592294940222E-4
62-63	1.470796147470111E-4	0.0010663272069158305	0.0	0.09166736989107468	2.941592294940222E-4
64-65	1.470796147470111E-4	0.0012134068216628417	0.0	0.09638311003890071	2.941592294940222E-4
66-67	1.470796147470111E-4	0.0012134068216628417	0.0	0.1015492815068895	2.941592294940222E-4
68-69	1.562720906686993E-4	0.00122259929758453	0.0	0.10637533136577579	2.941592294940222E-4
70-71	1.654645665903875E-4	0.0012317917735062182	0.0	0.11105430160991508	2.941592294940222E-4
72-73	1.746570425120757E-4	0.0012317917735062182	0.0	0.11468532959898192	2.941592294940222E-4
74-75	1.838495184337639E-4	0.0012777541531146588	0.0	0.11756257456247032	2.941592294940222E-4
76-77	1.838495184337639E-4	0.0013421014845664764	0.0	0.12065124647215755	2.941592294940222E-4
78-79	1.838495184337639E-4	0.0013421014845664764	0.0	0.12234266204174818	2.941592294940222E-4
80-81	2.0223447027714028E-4	0.0013512939604881646	0.0	0.12283905574151935	3.033517054157104E-4
82-83	2.0223447027714028E-4	0.0013604864364098529	0.0	0.12324352468207361	3.125441813373986E-4
84-85	2.0223447027714028E-4	0.0013604864364098529	0.0	0.12353768391156765	3.125441813373986E-4
86-87	2.0223447027714028E-4	0.0013880638641749173	0.0	0.12359283876709778	3.125441813373986E-4
88	2.0223447027714028E-4	0.0014707961474701111	0.0	0.12360203124301947	3.30929133180775E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10320	0.0	41.87748	1
CTTATAC	4810	0.0	36.542892	1
GTATCAA	17505	0.0	35.241707	1
ATCAACG	20000	0.0	30.054937	3
TCAACGC	20115	0.0	29.925526	4
TATCAAC	20630	0.0	29.469067	2
CAACGCA	20565	0.0	29.22921	5
TATACAC	7415	0.0	28.824472	3
AACGCAG	21260	0.0	28.353958	6
ACGCAGA	23680	0.0	25.438284	7
TTATACA	7535	0.0	25.422243	2
CGCAGAG	24265	0.0	24.807415	8
ACATCTC	8225	0.0	22.977493	8
ACACATC	8180	0.0	22.84313	6
TACACAT	9270	0.0	22.366156	5
GCAGAGT	27325	0.0	22.15425	9
GTACATG	18970	0.0	21.252756	1
TACATGG	18600	0.0	20.918604	2
GAGTACT	15440	0.0	20.902412	12-13
TATGCCG	5285	0.0	20.7474	42-43
>>END_MODULE