FastQCFastQC Report
Fri 27 May 2016
SRR2139441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8378128
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99300.049.014031
CTTATAC85650.041.5454331
GTATCAA191800.040.1152881
ATCAACG222850.033.9875873
CAACGCA230050.032.8680885
TCAACGC232600.032.58134
TATCAAC242850.031.6121252
AACGCAG240250.031.5946146
TATACAC123250.031.5074563
ACGCAGA264550.028.660197
TTATACA131200.028.2626322
CGCAGAG268750.028.2282058
ACACATC148150.024.4777976
ACATCTC148300.024.1935128
GCAGAGT320800.023.7014839
TACACAT174900.022.2029385
CATCTCC165850.021.5560389
CACATCT168800.021.2806537
GAGTACT203050.020.34474412-13
TATGCCG102600.020.08033642-43
Produced by FastQC (version 0.11.5)