##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8378128
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.798971202158764	32.0	30.0	33.0	18.0	33.0
2	31.433502567638023	33.0	31.0	33.0	28.0	33.0
3	31.09519763842233	33.0	31.0	33.0	28.0	33.0
4	32.11379152956364	33.0	33.0	33.0	30.0	34.0
5	32.21665961656351	33.0	33.0	33.0	31.0	34.0
6	35.74690718499407	38.0	36.0	38.0	31.0	38.0
7	36.793411845701094	38.0	37.0	38.0	35.0	38.0
8	36.703419785422234	38.0	38.0	38.0	34.0	38.0
9	37.04975681918443	38.0	38.0	38.0	36.0	38.0
10-11	37.17456387632177	38.0	38.0	38.0	36.0	38.0
12-13	37.23063200991916	38.0	38.0	38.0	36.0	38.0
14-15	37.277799646890095	38.0	38.0	38.0	37.0	38.0
16-17	37.284991706977976	38.0	38.0	38.0	37.0	38.0
18-19	37.31118538652071	38.0	38.0	38.0	37.0	38.0
20-21	37.28719915979667	38.0	38.0	38.0	37.0	38.0
22-23	37.311124669171406	38.0	38.0	38.0	37.0	38.0
24-25	37.33436242376738	38.0	38.0	38.0	37.0	38.0
26-27	37.30881454458955	38.0	38.0	38.0	37.0	38.0
28-29	37.28664246373525	38.0	38.0	38.0	37.0	38.0
30-31	37.28170979101809	38.0	38.0	38.0	37.0	38.0
32-33	37.21496759235853	38.0	38.0	38.0	36.5	38.0
34-35	37.22250195876359	38.0	38.0	38.0	37.0	38.0
36-37	37.21439660061655	38.0	38.0	38.0	37.0	38.0
38-39	37.17778212453876	38.0	38.0	38.0	36.0	38.0
40-41	37.20300423782959	38.0	38.0	38.0	37.0	38.0
42-43	37.17534110938817	38.0	38.0	38.0	36.0	38.0
44-45	37.14812132347224	38.0	38.0	38.0	36.0	38.0
46-47	37.117292166475046	38.0	38.0	38.0	36.0	38.0
48-49	37.075969252772566	38.0	38.0	38.0	36.0	38.0
50-51	37.05079097141234	38.0	38.0	38.0	36.0	38.0
52-53	37.01953091710388	38.0	38.0	38.0	36.0	38.0
54-55	36.99472383804505	38.0	38.0	38.0	36.0	38.0
56-57	36.96404643796192	38.0	38.0	38.0	36.0	38.0
58-59	36.96430880752861	38.0	38.0	38.0	36.0	38.0
60-61	36.983699987924055	38.0	38.0	38.0	36.0	38.0
62-63	36.98913663536083	38.0	38.0	38.0	36.0	38.0
64-65	36.984714314695125	38.0	38.0	38.0	36.0	38.0
66-67	36.98659928931587	38.0	38.0	38.0	36.0	38.0
68-69	36.987473555349425	38.0	38.0	38.0	36.0	38.0
70-71	36.97530886644669	38.0	38.0	38.0	36.0	38.0
72-73	36.951941328145836	38.0	38.0	38.0	36.0	38.0
74-75	36.94946819997543	38.0	38.0	38.0	36.0	38.0
76-77	36.95025206490647	38.0	38.0	38.0	36.0	38.0
78-79	36.92833744110119	38.0	38.0	38.0	36.0	38.0
80-81	36.90479557992292	38.0	38.0	38.0	35.5	38.0
82-83	36.90573160045548	38.0	38.0	38.0	35.0	38.0
84-85	36.85336413880135	38.0	38.0	38.0	35.0	38.0
86-87	36.83998204231804	38.0	38.0	38.0	35.0	38.0
88-89	36.849203022644176	38.0	38.0	38.0	35.0	38.0
90-91	36.82574379966266	38.0	38.0	38.0	35.0	38.0
92-93	36.830686155163306	38.0	38.0	38.0	35.0	38.0
94-95	36.78218017616455	38.0	38.0	38.0	35.0	38.0
96-97	36.75686161632699	38.0	38.0	38.0	35.0	38.0
98-99	36.83425108975672	38.0	38.0	38.0	35.0	38.0
100	35.16687391804353	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	10.0
18	46.0
19	76.0
20	111.0
21	278.0
22	794.0
23	2032.0
24	4703.0
25	9264.0
26	16070.0
27	25844.0
28	39548.0
29	56952.0
30	78107.0
31	102324.0
32	132974.0
33	179980.0
34	265372.0
35	457525.0
36	1221466.0
37	5784652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.15541753480014	20.794991434840814	14.561259985524213	25.48833104483484
2	13.10901594798996	20.825596230854888	44.405945102609586	21.65944271854557
3	16.66730324482987	26.92353232130137	31.50804093706852	24.90112349680024
4	11.072580891578644	19.14779769418658	41.2185156397706	28.561105774464174
5	12.295523714611784	37.74234475092935	36.96861053348521	12.99352100097366
6	27.444257913045867	39.92635557367158	19.894474817962795	12.73491169531976
7	24.665489076496453	33.513206472043215	24.339306386737753	17.481998064722582
8	22.794196985293137	37.709247220858884	22.406568627263752	17.08998716658423
9	24.631087039968833	17.13292038507886	22.36497222291185	35.87102035204045
10-11	22.903976878844535	27.49627959849742	30.173464764443796	19.426278758214245
12-13	24.050754536096846	24.917165266512995	30.014449528582045	21.017630668808117
14-15	21.18568133597386	26.31309762753684	27.758826315377373	24.742394721111925
16-17	20.681051900854225	29.502974888901196	28.348426999444264	21.46754621080031
18-19	20.52561144924021	28.580799911388322	30.27662623440463	20.616962404966838
20-21	21.480993296891366	27.75876066046242	29.964853693525235	20.795392349120974
22-23	21.09000430014345	28.012901027170283	29.79822151319094	21.098873159495334
24-25	20.964346434867313	28.06226757617294	30.14337645997453	20.830009528985215
26-27	21.065779074570628	28.291878675583977	29.89283949084432	20.749502759001075
28-29	20.702949605923095	28.269548602818244	30.085604251253883	20.941897540004778
30-31	21.152631121959363	28.36215848335815	29.81939415763576	20.66581623704673
32-33	21.434016083252512	27.96260902105348	29.805979326355835	20.79739556933817
34-35	21.035412033114003	28.103411714853216	29.94626408997036	20.91491216206243
36-37	20.89521333412009	28.393662897047367	29.876482850058583	20.834640918773957
38-39	20.809736646721486	27.987562536150577	30.167097018089894	21.035603799038043
40-41	21.015342147315945	27.891140221450307	30.14613854231999	20.947379088913756
42-43	21.237312800316968	27.837049825229737	29.993758095400942	20.93187927905236
44-45	21.037052448061875	28.365310295268838	29.711157838070097	20.886479418599198
46-47	21.271699967959133	27.9069118741617	29.32667851253162	21.494709645347555
48-49	21.304542797935806	27.852771315416142	29.4632699124939	21.379415974154153
50-51	20.945300309944802	28.081114218739128	29.490175206201	21.483410265115072
52-53	21.08125774420018	28.147832835600077	29.607208427475385	21.16370099272436
54-55	21.165851769876685	28.05717988515535	29.332751232077563	21.444217112890406
56-57	21.528393983518235	28.332296035684074	28.865189896695	21.274120084102695
58-59	21.028920795707208	28.67003430636878	29.464262224406124	20.836782673517888
60-61	21.336427036331262	29.024996461988593	28.78884160569768	20.849734895982465
62-63	21.05539386579435	29.178959870668248	28.71135409889573	21.054292164641673
64-65	20.91303016416191	29.211012081939618	28.779494162465895	21.096463591432574
66-67	21.12288886761116	29.11227922393888	28.63301675540133	21.13181515304863
68-69	21.130811912238766	28.970964288600516	28.82389311849152	21.074330680669203
70-71	21.106352359134416	28.52078863868679	28.95661608159311	21.416242920585677
72-73	21.320798089367003	28.270131151211835	29.19154426286006	21.217526496561103
74-75	21.275558789136863	28.420317801119488	28.95537092484665	21.348752484897
76-77	21.188966345763035	28.446925973684817	28.88498467072652	21.479123009825624
78-79	21.254182307766452	28.403706914554476	28.895264625920515	21.446846151758553
80-81	21.179046443279606	28.41054205342262	28.95344448799346	21.45696701530431
82-83	21.337346934956095	28.299106040328546	29.083938119686596	21.27960890502876
84-85	21.722241379885094	28.27356945039649	28.7432750121397	21.26091415757871
86-87	21.238947715009225	28.45681883087937	28.88219303836444	21.42204041574696
88-89	21.420596382821312	28.306114414985007	28.86389186569391	21.409397336499776
90-91	21.56605211389592	28.333863839308876	28.803872805521085	21.29621124127412
92-93	21.558393557589582	28.344035398964223	28.79469481207002	21.302876231376175
94-95	21.359040220767934	28.5106661625591	28.808679485030737	21.32161413164223
96-97	21.362828392025953	28.64184225529885	28.886441680793396	21.108887671881803
98-99	22.38486838037059	30.552251201316498	29.17317338012794	17.889707038184973
100	21.299016628776464	31.61610763934089	23.0057119390458	24.079163792836848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	914.0
1	1058.5
2	1993.5
3	3137.0
4	4344.0
5	6231.5
6	7519.5
7	8675.0
8	10045.5
9	10864.5
10	11121.0
11	11415.0
12	12942.0
13	16382.0
14	22800.5
15	30119.5
16	35206.5
17	37646.5
18	37056.5
19	34291.0
20	31365.5
21	29759.5
22	31747.0
23	38020.0
24	47903.0
25	62026.5
26	81119.0
27	102563.0
28	121935.0
29	143554.0
30	165775.5
31	185548.5
32	209607.0
33	233722.5
34	257826.5
35	283536.0
36	307994.0
37	329442.0
38	340788.5
39	344686.0
40	352414.5
41	356295.0
42	358663.0
43	377970.0
44	392749.5
45	388379.0
46	373186.0
47	356111.0
48	341938.0
49	326249.0
50	310221.5
51	291684.5
52	269942.0
53	249748.5
54	230160.0
55	205134.5
56	178896.0
57	152872.5
58	127480.5
59	107130.5
60	87884.5
61	67707.5
62	49384.0
63	35323.5
64	25002.0
65	17326.0
66	12094.5
67	8634.5
68	6184.5
69	4303.5
70	3083.0
71	2186.5
72	1555.0
73	1048.0
74	658.0
75	430.0
76	272.0
77	158.5
78	96.0
79	73.5
80	55.0
81	42.5
82	36.5
83	24.0
84	14.5
85	9.5
86	4.0
87	2.0
88	2.0
89	1.5
90	1.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0024707189959379946
3	0.0
4	0.0
5	0.0053114490492386845
6	3.9388273848286874E-4
7	1.1935840560086931E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.805633348615821E-5
44-45	1.8520664400437013E-5
46-47	0.0
48-49	0.0
50-51	6.3301836449577236E-6
52-53	9.756879604505841E-4
54-55	0.0020684894855531747
56-57	0.0023503757590402543
58-59	0.0015406325850450403
60-61	0.0036925602020600537
62-63	0.0015795224864604063
64-65	0.0014789021888220826
66-67	1.9595894808696092E-4
68-69	1.5711977322288313E-4
70-71	1.7277158518344058E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	9.358196188968183E-5
80-81	2.9075667097568054E-4
82-83	7.3392685508819746E-6
84-85	1.4815805827604619E-5
86-87	0.0
88-89	0.0
90-91	4.5777100615778295E-5
92-93	2.3900241932126395E-4
94-95	0.0
96-97	0.0
98-99	8.529199287300108E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	92.0
20-21	291.0
22-23	529.0
24-25	729.0
26-27	1635.0
28-29	4388.0
30-31	14188.0
32-33	18073.0
34-35	24959.0
36-37	40717.0
38-39	50836.0
40-41	49903.0
42-43	56469.0
44-45	67067.0
46-47	69542.0
48-49	65117.0
50-51	58979.0
52-53	56454.0
54-55	60202.0
56-57	63127.0
58-59	62265.0
60-61	63474.0
62-63	61375.0
64-65	68619.0
66-67	78337.0
68-69	81806.0
70-71	99613.0
72-73	68329.0
74-75	62657.0
76-77	65883.0
78-79	67149.0
80-81	66641.0
82-83	63547.0
84-85	62992.0
86-87	64343.0
88-89	67095.0
90-91	68442.0
92-93	68594.0
94-95	81870.0
96-97	241122.0
98-99	548994.0
100-101	5561684.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.97811463498853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.9047901000661	69.50481264952703
2	8.192731710963157	13.104784719062343
3	2.533047095669804	6.07764992979913
4	1.0538492060830196	3.3713949044839757
5	0.5063113224390581	2.0246912493511813
6	0.2818788886453894	1.352648524155847
7	0.16651742812958256	0.9322424968969851
8	0.10450615967804212	0.6686564495034295
9	0.07056866616384536	0.5079553984881234
>10	0.1829186904976312	2.0428265688369773
>50	0.0017770991380266932	0.09347586532852345
>100	8.882692484376113E-4	0.14956772196276216
>500	1.615226598178682E-4	0.08541125708821073
>1k	5.3840618082570307E-5	0.08388226551551516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1935840560086931E-5	0.0
4	0.0	1.1935840560086931E-5	0.0	1.1935840560086931E-5	0.0
5	0.0	8.355088392060851E-5	0.0	1.1935840560086931E-5	0.0
6	0.0	8.355088392060851E-5	0.0	1.1935840560086931E-5	0.0
7	0.0	8.355088392060851E-5	0.0	2.3871681120173862E-5	0.0
8	0.0	8.355088392060851E-5	0.0	2.3871681120173862E-5	0.0
9	0.0	8.355088392060851E-5	0.0	7.161504336052159E-5	0.0
10-11	0.0	8.951880420065197E-5	0.0	7.161504336052159E-5	0.0
12-13	0.0	1.4323008672104316E-4	0.0	9.548672448069545E-5	0.0
14-15	0.0	1.8500552868134742E-4	0.0	1.551659272811301E-4	0.0
16-17	0.0	2.1484513008156476E-4	0.0	1.9694136924143437E-4	0.0
18-19	0.0	2.3871681120173863E-4	0.0	2.267809706416517E-4	0.0
20-21	0.0	2.6258849232191247E-4	0.0	2.56620572041869E-4	0.0
22-23	0.0	2.745243328819994E-4	0.0	2.804922531620429E-4	0.0
24-25	1.1935840560086931E-5	2.8646017344208637E-4	0.0	3.103318545622602E-4	0.0
26-27	2.3871681120173862E-5	2.9242809372212984E-4	0.0	3.46139376242521E-4	0.0
28-29	2.3871681120173862E-5	3.0436393428221674E-4	0.0	4.177544196030426E-4	0.0
30-31	2.3871681120173862E-5	3.46139376242521E-4	0.0	9.668030853670414E-4	0.0
32-33	2.3871681120173862E-5	4.058185790429557E-4	0.0	0.002536366119018473	0.0
34-35	2.3871681120173862E-5	4.416261007232164E-4	0.0	0.004487876050592686	0.0
36-37	2.3871681120173862E-5	5.848561874442597E-4	0.0	0.00752554747313481	0.0
38-39	2.3871681120173862E-5	6.146957888444769E-4	0.0	0.012407306262210364	0.0
40-41	2.3871681120173862E-5	6.445353902446943E-4	0.0	0.017629236507248396	0.0
42-43	2.3871681120173862E-5	6.445353902446943E-4	0.0	0.0228332629914463	0.0
44-45	2.3871681120173862E-5	6.445353902446943E-4	0.0	0.02879521535120972	0.0
46-47	2.3871681120173862E-5	6.505033105247378E-4	0.0	0.03473329602985297	0.0
48-49	2.3871681120173862E-5	6.564712308047813E-4	0.0	0.04086831807773765	0.0
50-51	2.3871681120173862E-5	7.579258755655202E-4	0.0	0.04730770405990455	0.0
52-53	2.3871681120173862E-5	8.653484406063025E-4	0.0	0.05365160331759075	0.0
54-55	2.3871681120173862E-5	9.608351650869979E-4	0.0	0.059744849923515135	0.0
56-57	2.3871681120173862E-5	0.001038418128727563	0.0	0.06448934654614968	0.0
58-59	2.3871681120173862E-5	0.0011339048532082584	0.0	0.06943675245830572	0.0
60-61	2.3871681120173862E-5	0.0011697123748885192	0.0	0.07441996589214202	0.0
62-63	2.3871681120173862E-5	0.00120551989656878	0.0	0.07936737180429805	0.0
64-65	2.3871681120173862E-5	0.0012532632588091277	0.0	0.08415961178917294	0.0
66-67	2.3871681120173862E-5	0.0012651990993692145	0.0	0.08910701770132898	0.0
68-69	2.3871681120173862E-5	0.001283102860209345	0.0	0.09400071233096463	0.0
70-71	2.3871681120173862E-5	0.0013010066210494757	0.0	0.09805889812139418	0.0
72-73	2.3871681120173862E-5	0.0013129424616095625	0.0	0.10140690139849856	0.0
74-75	2.3871681120173862E-5	0.0013129424616095625	0.0	0.10417601640843874	0.0
76-77	2.3871681120173862E-5	0.0013129424616095625	0.0	0.10664673540437672	0.0
78-79	2.3871681120173862E-5	0.0013129424616095625	0.0	0.1083177530827889	0.0
80-81	2.3871681120173862E-5	0.0013487499832898233	0.0	0.10888470550939303	0.0
82-83	2.3871681120173862E-5	0.0013487499832898233	0.0	0.10936810705207656	0.0
84-85	2.3871681120173862E-5	0.0013487499832898233	0.0	0.1095531125807579	0.0
86-87	2.3871681120173862E-5	0.0013487499832898233	0.0	0.10966053514579868	0.0
88	2.3871681120173862E-5	0.0013487499832898233	0.0	0.10966650306607872	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9930	0.0	49.01403	1
CTTATAC	8565	0.0	41.545433	1
GTATCAA	19180	0.0	40.115288	1
ATCAACG	22285	0.0	33.987587	3
CAACGCA	23005	0.0	32.868088	5
TCAACGC	23260	0.0	32.5813	4
TATCAAC	24285	0.0	31.612125	2
AACGCAG	24025	0.0	31.594614	6
TATACAC	12325	0.0	31.507456	3
ACGCAGA	26455	0.0	28.66019	7
TTATACA	13120	0.0	28.262632	2
CGCAGAG	26875	0.0	28.228205	8
ACACATC	14815	0.0	24.477797	6
ACATCTC	14830	0.0	24.193512	8
GCAGAGT	32080	0.0	23.701483	9
TACACAT	17490	0.0	22.202938	5
CATCTCC	16585	0.0	21.556038	9
CACATCT	16880	0.0	21.280653	7
GAGTACT	20305	0.0	20.344744	12-13
TATGCCG	10260	0.0	20.080336	42-43
>>END_MODULE