FastQCFastQC Report
Fri 27 May 2016
SRR2139440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7765860
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190200.051.8527871
GTATCAA353450.038.687861
ATCAACG424300.031.668723
TATCAAC449450.030.1694372
ACGCAGA447850.030.1133087
AACGCAG453500.029.7031976
TCAACGC455050.029.577014
CAACGCA455000.029.5380385
CGCAGAG500350.027.6451478
GCAGAGT530700.024.465519
GAGTACT317450.023.705312-13
TACATGG404750.023.6750932
ACATGGG403200.023.282973
GTACATG416800.023.2349261
GTGGTAT78850.022.5312481
CAGAGTA516800.021.74363510-11
TGGTATC78500.021.659322
AGAGTAC497850.021.16553910-11
AGTACTT333100.021.10077912-13
GTACTTT349900.021.01264414-15
Produced by FastQC (version 0.11.5)