FastQCFastQC Report
Fri 27 May 2016
SRR2139439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8870228
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101600.047.4914971
GTATCAA191100.036.4888271
TCAACGC244900.031.2714794
ATCAACG245950.031.1203373
CAACGCA251000.030.4769215
TATCAAC257100.029.9740642
AACGCAG257600.029.7285826
ACGCAGA297100.025.6883457
CGCAGAG300900.025.3495128
CTTATAC86400.024.307281
GCAGAGT348450.021.890289
TATACAC118600.020.926853
GTGGTAT51150.020.1899871
AGAGTAC316450.019.79176910-11
GAGTACT213700.019.74503912-13
TGGTATC54100.019.7307032
TTATACA119850.018.9351352
CAGAGTA343500.018.77634410-11
TACATGG234500.018.3002992
AGTACTT235900.017.96045112-13
Produced by FastQC (version 0.11.5)