FastQCFastQC Report
Fri 27 May 2016
SRR2139438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7292314
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90150.050.2841721
GTATCAA169600.035.8321341
TCAACGC220600.030.4717884
ATCAACG222200.030.2910823
CAACGCA227900.029.4768585
TATCAAC230800.029.38682
AACGCAG234700.028.6582896
CTTATAC65200.025.8579941
ACGCAGA263150.025.5436067
CGCAGAG271200.024.78548
TGGTATC42000.022.1187652
GCAGAGT311200.021.6134349
TATACAC90000.021.2648333
GTGGTAT40700.021.0288281
TACATGG217700.020.6252162
GAGTACT187250.020.50955612-13
ACATGGG219950.019.7293013
AGAGTAC283550.019.51980810-11
CAGAGTA304650.019.41011610-11
GTACATG212650.019.132961
Produced by FastQC (version 0.11.5)