##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7292314
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.816645717669317	18.0	18.0	31.0	18.0	33.0
2	30.77093512429662	32.0	29.0	33.0	27.0	33.0
3	31.023296034701744	33.0	31.0	33.0	27.0	33.0
4	32.207429356442965	33.0	33.0	33.0	30.0	34.0
5	32.58769795156928	33.0	33.0	34.0	31.0	34.0
6	36.43265498441235	38.0	37.0	38.0	34.0	38.0
7	36.72427709503458	38.0	37.0	38.0	34.0	38.0
8	36.724320016938385	38.0	38.0	38.0	34.0	38.0
9	37.1146963775833	38.0	38.0	38.0	36.0	38.0
10-11	37.173208668743555	38.0	38.0	38.0	36.0	38.0
12-13	37.22719551023173	38.0	38.0	38.0	36.0	38.0
14-15	37.2282740430541	38.0	38.0	38.0	36.0	38.0
16-17	37.23605531796903	38.0	38.0	38.0	36.0	38.0
18-19	37.27506892873785	38.0	38.0	38.0	37.0	38.0
20-21	37.26307870619522	38.0	38.0	38.0	37.0	38.0
22-23	37.296102674861025	38.0	38.0	38.0	37.0	38.0
24-25	37.31783361138841	38.0	38.0	38.0	37.0	38.0
26-27	37.30007045653459	38.0	38.0	38.0	37.0	38.0
28-29	37.2784427695719	38.0	38.0	38.0	37.0	38.0
30-31	37.274220522423896	38.0	38.0	38.0	37.0	38.0
32-33	37.214348113933035	38.0	38.0	38.0	36.5	38.0
34-35	37.22048391594969	38.0	38.0	38.0	37.0	38.0
36-37	37.21764949992304	38.0	38.0	38.0	37.0	38.0
38-39	37.19919841523226	38.0	38.0	38.0	36.5	38.0
40-41	37.19519794876503	38.0	38.0	38.0	36.5	38.0
42-43	37.169891200382665	38.0	38.0	38.0	36.0	38.0
44-45	37.14254428047865	38.0	38.0	38.0	36.0	38.0
46-47	37.10390638565572	38.0	38.0	38.0	36.0	38.0
48-49	37.052671885854906	38.0	38.0	38.0	36.0	38.0
50-51	37.021123054687266	38.0	38.0	38.0	36.0	38.0
52-53	36.98333080348979	38.0	38.0	38.0	36.0	38.0
54-55	36.95852526508343	38.0	38.0	38.0	36.0	38.0
56-57	36.92684062330099	38.0	38.0	38.0	36.0	38.0
58-59	36.933561512947406	38.0	38.0	38.0	36.0	38.0
60-61	36.95637792123031	38.0	38.0	38.0	36.0	38.0
62-63	36.96532033817488	38.0	38.0	38.0	36.0	38.0
64-65	36.96273836193744	38.0	38.0	38.0	36.0	38.0
66-67	36.967739538036525	38.0	38.0	38.0	36.0	38.0
68-69	36.96963561739339	38.0	38.0	38.0	36.0	38.0
70-71	36.95784269455173	38.0	38.0	38.0	36.0	38.0
72-73	36.93243697157645	38.0	38.0	38.0	36.0	38.0
74-75	36.929168941913815	38.0	38.0	38.0	36.0	38.0
76-77	36.92667345021112	38.0	38.0	38.0	36.0	38.0
78-79	36.89939846148136	38.0	38.0	38.0	35.0	38.0
80-81	36.87560927876667	38.0	38.0	38.0	35.0	38.0
82-83	36.873486610707175	38.0	38.0	38.0	35.0	38.0
84-85	36.82042398304395	38.0	38.0	38.0	35.0	38.0
86-87	36.80419162686175	38.0	38.0	38.0	35.0	38.0
88-89	36.80735904649835	38.0	38.0	38.0	35.0	38.0
90-91	36.78596841246976	38.0	38.0	38.0	35.0	38.0
92-93	36.79373027730223	38.0	38.0	38.0	35.0	38.0
94-95	36.74222932648986	38.0	38.0	38.0	34.5	38.0
96-97	36.70933359274339	38.0	38.0	38.0	34.0	38.0
98-99	36.79049629601319	38.0	38.0	38.0	35.0	38.0
100	35.14446091021774	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	11.0
18	21.0
19	59.0
20	109.0
21	274.0
22	827.0
23	1997.0
24	4425.0
25	8569.0
26	14387.0
27	23112.0
28	34648.0
29	50410.0
30	69696.0
31	92344.0
32	120389.0
33	162363.0
34	239431.0
35	422595.0
36	1221037.0
37	4825609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.007373242567446	19.007697693763596	18.923622871971777	24.061306191697177
2	14.437824560364183	20.89465251231046	41.64822223063307	23.019300696692287
3	17.78120086436212	25.67761070080087	31.124427719376868	25.41676071546014
4	12.360671797731145	17.948980255101468	38.29104177357146	31.399306173595924
5	13.343451286062006	37.0778672321091	35.6370257826774	13.941655699151493
6	30.04225004456756	37.74617061832344	18.530641909959822	13.680937427149184
7	26.395338982934636	32.54257839144063	22.913426382901232	18.148656242723504
8	24.385880805461753	36.70022985845097	20.995585214789163	17.918304121298124
9	25.46985771594586	16.73356358489226	21.192559727954666	36.60401897120722
10-11	24.260646209145683	26.68331341738713	28.707917678805384	20.348122694661804
12-13	25.0869614226705	24.23267154979887	28.82790702649392	21.852460001036707
14-15	22.221794069756186	25.45798219879177	26.573368892233656	25.746854839218386
16-17	21.820316568924486	28.67257910177757	27.18821487939219	22.31888944990575
18-19	21.874243210042792	27.55192659010569	29.089799205026008	21.484030994825513
20-21	22.69388607007607	26.688414177634645	29.015963754575026	21.601735997714265
22-23	22.386390682627887	26.70228065133725	28.88627545042557	22.02505321560929
24-25	22.115689646021085	26.848917948589236	29.07656314868464	21.958829256705037
26-27	22.091058635769915	26.922833448954965	29.03927511924231	21.946832796032812
28-29	21.965326841881172	26.783167760779687	29.08501754198681	22.166487855352326
30-31	22.14556577661843	26.680297598921538	29.064992024970753	22.10914459948928
32-33	21.993584826672627	26.871797856073865	29.00221430855634	22.132403008697167
34-35	22.107856179534203	27.007915204179007	29.045172089485526	21.83905652680126
36-37	22.165167986944763	26.852057224857585	28.832356759708095	22.150418028489558
38-39	21.998696835211955	26.9181960589243	29.04068172859643	22.042425377267318
40-41	22.231262370338023	26.80087895689136	28.93777979975058	22.030078873020038
42-43	22.216331425652772	26.855052465903068	28.78555947316966	22.1430566352745
44-45	22.13334464144999	27.03943429588957	28.692184451334874	22.135036611325564
46-47	22.360837325767037	26.88698773677923	28.378458026036885	22.37371691141685
48-49	22.283191950720457	27.02942604292901	28.294742292879565	22.392639713470963
50-51	22.172052810088857	27.163205699363722	28.276671515592923	22.3880699749545
52-53	22.36479823954434	27.17884325376766	28.251839752901304	22.204518753786697
54-55	22.324508827846905	27.085730618279385	28.176237777472767	22.41352277640094
56-57	22.468212878384346	27.250958871156904	27.96405676526125	22.3167714851975
58-59	22.228167496334265	27.39114986942881	28.220414988083242	22.160267646153684
60-61	22.38068472750088	27.525922032871534	27.98500780079955	22.10838543882803
62-63	22.261612686670798	27.70006616285573	27.905414829293317	22.132906321180155
64-65	22.234400449316276	27.594556482607086	27.959015308537786	22.21202775953885
66-67	22.333752505715356	27.603102081002955	27.83545933416901	22.227686079112676
68-69	22.280582639797856	27.484031379612585	27.95616435799263	22.27922162259693
70-71	22.33004292486224	27.225968009466573	27.978099912861666	22.465889152809524
72-73	22.423617080710535	27.127100596302917	27.932816818638116	22.516465504348425
74-75	22.30847382913577	27.24400638349131	27.932541504591047	22.514978282781872
76-77	22.469407812656485	27.176598291191052	27.797652693387185	22.55634120276528
78-79	22.379201640819716	27.226458723761375	27.809325691992363	22.585013943426542
80-81	22.477276627125466	27.236496648489393	27.710977858175113	22.575248866210025
82-83	22.49113157995849	27.21449359396267	27.817622669622722	22.47675215645611
84-85	22.57049973653195	27.22967917665688	27.704106324592974	22.495714762218196
86-87	22.437907634628235	27.28931300807533	27.75450222119547	22.51827713610097
88-89	22.49468329803692	27.203140185918194	27.699610915444467	22.602565600600418
90-91	22.52899294435021	27.262424473505277	27.689683458474818	22.518899123669698
92-93	22.536586260357865	27.269775834796317	27.688591862850696	22.50504604199512
94-95	22.4847569992614	27.341169706850227	27.683727797643083	22.49034549624529
96-97	22.5235517307649	27.466229343892977	27.74196545402385	22.26825347131827
98-99	23.651656079348943	29.342679490761686	27.934366838585756	19.07129759130362
100	22.4906421268766	30.199064499801892	21.918915659784403	25.391377713537107
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2567.0
1	2676.0
2	3086.5
3	3507.0
4	4076.0
5	4983.0
6	5957.0
7	9209.0
8	12160.0
9	12213.0
10	11706.5
11	11564.0
12	12553.5
13	15145.5
14	20341.0
15	26043.0
16	29513.5
17	30201.0
18	28426.5
19	25849.5
20	23674.0
21	22648.5
22	23975.0
23	28168.5
24	35016.0
25	45137.0
26	57930.5
27	72075.5
28	87304.5
29	103238.0
30	117415.5
31	131768.5
32	147564.0
33	162093.0
34	176050.5
35	189950.0
36	205565.5
37	220503.0
38	231585.0
39	240485.0
40	251232.5
41	262168.0
42	270194.0
43	282940.0
44	295277.5
45	300083.5
46	299936.0
47	298353.0
48	297719.0
49	295456.5
50	291477.0
51	285932.0
52	275912.5
53	261327.5
54	247144.5
55	232571.5
56	215697.0
57	197126.5
58	176894.5
59	155645.0
60	132739.5
61	109643.5
62	88380.0
63	69913.0
64	54410.0
65	42226.0
66	32372.0
67	23756.5
68	17130.5
69	12578.0
70	9298.0
71	6614.0
72	4755.5
73	3293.5
74	2203.5
75	1442.0
76	901.0
77	527.5
78	314.5
79	188.0
80	112.5
81	70.0
82	42.5
83	24.5
84	18.5
85	17.5
86	12.5
87	7.0
88	4.5
89	2.5
90	1.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001947255699631146
3	0.0
4	0.0
5	0.004456747199860017
6	1.9198295630166225E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.437738629795624E-5
44-45	1.4158741723949984E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.00101390900801258
54-55	0.0021883983310892457
56-57	0.0026037211169105544
58-59	0.0017515022299232008
60-61	0.00391376878130123
62-63	0.0018474981165362441
64-65	0.001564766167831934
66-67	1.7713647186814823E-4
68-69	1.5554458102122796E-4
70-71	1.728620422613121E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	8.159053902218471E-5
80-81	4.942659791866243E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.45678670934646E-5
92-93	3.667453801215447E-4
94-95	0.0
96-97	0.0
98-99	9.6950490196221E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	80.0
20-21	310.0
22-23	589.0
24-25	732.0
26-27	1767.0
28-29	4488.0
30-31	11988.0
32-33	15431.0
34-35	21870.0
36-37	34211.0
38-39	39639.0
40-41	39664.0
42-43	45533.0
44-45	54556.0
46-47	55890.0
48-49	52548.0
50-51	47366.0
52-53	45123.0
54-55	48583.0
56-57	50452.0
58-59	52226.0
60-61	53021.0
62-63	52220.0
64-65	56557.0
66-67	62082.0
68-69	64072.0
70-71	71940.0
72-73	55251.0
74-75	54174.0
76-77	57069.0
78-79	58610.0
80-81	58294.0
82-83	55335.0
84-85	54500.0
86-87	56432.0
88-89	58904.0
90-91	59916.0
92-93	59918.0
94-95	73246.0
96-97	228071.0
98-99	503548.0
100-101	4876108.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.31189003585207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.73571833669193	78.315713434006
2	6.542712009505208	11.294276790013246
3	1.5203225718848357	3.936657439306432
4	0.5349616064750498	1.8469418940590905
5	0.2516167981633991	1.0858760707126243
6	0.13348851428540454	0.6912987579630664
7	0.07973514040229908	0.4817463469277524
8	0.05052793494092653	0.348892925148795
9	0.037041872807589867	0.2877438647241644
>10	0.11131363873823623	1.4934334891003127
>50	0.0021737629359432553	0.1212497175576144
>100	3.124448343094129E-4	0.0514352924955904
>500	7.536833487063151E-5	0.04473397798529191
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.7426136614523183E-5	0.0	1.3713068307261591E-5	0.0
3	0.0	2.7426136614523183E-5	0.0	1.3713068307261591E-5	0.0
4	0.0	8.227840984356955E-5	0.0	4.1139204921784775E-5	0.0
5	0.0	9.599147815083114E-5	0.0	5.4852273229046365E-5	0.0
6	1.3713068307261591E-5	9.599147815083114E-5	0.0	5.4852273229046365E-5	0.0
7	1.3713068307261591E-5	9.599147815083114E-5	0.0	5.4852273229046365E-5	0.0
8	1.3713068307261591E-5	1.0970454645809273E-4	0.0	5.4852273229046365E-5	1.3713068307261591E-5
9	1.3713068307261591E-5	2.0569602460892387E-4	0.0	6.856534153630795E-5	1.3713068307261591E-5
10-11	2.7426136614523183E-5	2.0569602460892387E-4	0.0	1.234176147653543E-4	1.3713068307261591E-5
12-13	4.1139204921784775E-5	2.1255255876255467E-4	0.0	1.234176147653543E-4	1.3713068307261591E-5
14-15	4.1139204921784775E-5	2.879744344524934E-4	0.0	1.302741489189851E-4	2.7426136614523183E-5
16-17	4.799573907541557E-5	3.0168750275975497E-4	0.0	1.371306830726159E-4	2.7426136614523183E-5
18-19	5.4852273229046365E-5	3.1540057106701656E-4	0.0	1.371306830726159E-4	2.7426136614523183E-5
20-21	5.4852273229046365E-5	3.35970173527909E-4	0.0	1.371306830726159E-4	2.7426136614523183E-5
22-23	5.4852273229046365E-5	3.8396591260332456E-4	0.0	1.5084375137987749E-4	2.7426136614523183E-5
24-25	6.856534153630796E-5	4.7310085660052484E-4	0.0	1.782698879944007E-4	4.1139204921784775E-5
26-27	8.227840984356955E-5	5.073835273686788E-4	0.0	2.1255255876255467E-4	6.170880738267715E-5
28-29	8.227840984356955E-5	5.073835273686788E-4	0.0	5.005269932150481E-4	6.856534153630795E-5
30-31	8.913494399720035E-5	5.896619372122484E-4	0.0	0.0014261591039552053	6.856534153630795E-5
32-33	9.599147815083114E-5	5.896619372122484E-4	0.0	0.003174575313131058	6.856534153630795E-5
34-35	9.599147815083114E-5	6.856534153630795E-4	0.0	0.005505796925365529	8.227840984356955E-5
36-37	9.599147815083114E-5	8.090710301284339E-4	0.0	0.009276890709862467	8.227840984356955E-5
38-39	9.599147815083114E-5	8.502102350502187E-4	0.0	0.019095447617861763	8.227840984356955E-5
40-41	1.0970454645809273E-4	9.05062508279265E-4	0.0	0.0297230755559895	8.227840984356955E-5
42-43	1.0970454645809273E-4	9.530582473546805E-4	0.0	0.03624363953609239	8.227840984356955E-5
44-45	1.0970454645809273E-4	0.0010970454645809272	0.0	0.04355956147801644	8.913494399720035E-5
46-47	1.0970454645809273E-4	0.0011793238744244967	0.0	0.05199309848698232	1.0284801230446194E-4
48-49	1.0970454645809273E-4	0.0011861804085781275	0.0	0.060385496291026415	1.234176147653543E-4
50-51	1.0970454645809273E-4	0.0013918764331870516	0.0	0.0685310588655398	1.234176147653543E-4
52-53	1.0970454645809273E-4	0.0013987329673406824	0.0	0.08776363716647417	1.302741489189851E-4
54-55	1.0970454645809273E-4	0.0014055895014943131	0.0	0.11070560044452282	1.439872172262467E-4
56-57	1.0970454645809273E-4	0.0014398721722624671	0.0	0.13917393025039787	1.5084375137987749E-4
58-59	1.0970454645809273E-4	0.0014604417747233595	0.0	0.16195133670875939	1.5084375137987749E-4
60-61	1.0970454645809273E-4	0.0015152940479524059	0.0	0.1711528055429319	1.5084375137987749E-4
62-63	1.0970454645809273E-4	0.0015564332528741906	0.0	0.1825072261013445	1.645568196871391E-4
64-65	1.0970454645809273E-4	0.0017004204701004373	0.0	0.18833528013193068	1.645568196871391E-4
66-67	1.0970454645809273E-4	0.0017552727433294837	0.0	0.1937245159766845	1.645568196871391E-4
68-69	1.0970454645809273E-4	0.001775842345790376	0.0	0.19883263392113942	1.645568196871391E-4
70-71	1.0970454645809273E-4	0.0017826988799440069	0.0	0.20332366379176758	1.645568196871391E-4
72-73	1.0970454645809273E-4	0.0017964119482512685	0.0	0.20699190956396007	1.782698879944007E-4
74-75	1.0970454645809273E-4	0.001844407687326684	0.0	0.2097550928278733	1.782698879944007E-4
76-77	1.234176147653543E-4	0.0018992599605557304	0.0	0.2126074110357837	1.782698879944007E-4
78-79	1.234176147653543E-4	0.0019335426313238843	0.0	0.21436954031326683	1.782698879944007E-4
80-81	1.234176147653543E-4	0.0019403991654775151	0.0	0.21486321077232823	1.782698879944007E-4
82-83	1.234176147653543E-4	0.0019609687679384073	0.0	0.21521289401416338	1.782698879944007E-4
84-85	1.234176147653543E-4	0.0019883949045529305	0.0	0.2153911639021578	1.782698879944007E-4
86-87	1.371306830726159E-4	0.002036390643628346	0.0	0.21549401191446227	1.782698879944007E-4
88	1.371306830726159E-4	0.0021803778608545927	0.0	0.2155008684486159	1.782698879944007E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9015	0.0	50.284172	1
GTATCAA	16960	0.0	35.832134	1
TCAACGC	22060	0.0	30.471788	4
ATCAACG	22220	0.0	30.291082	3
CAACGCA	22790	0.0	29.476858	5
TATCAAC	23080	0.0	29.3868	2
AACGCAG	23470	0.0	28.658289	6
CTTATAC	6520	0.0	25.857994	1
ACGCAGA	26315	0.0	25.543606	7
CGCAGAG	27120	0.0	24.7854	8
TGGTATC	4200	0.0	22.118765	2
GCAGAGT	31120	0.0	21.613434	9
TATACAC	9000	0.0	21.264833	3
GTGGTAT	4070	0.0	21.028828	1
TACATGG	21770	0.0	20.625216	2
GAGTACT	18725	0.0	20.509556	12-13
ACATGGG	21995	0.0	19.729301	3
AGAGTAC	28355	0.0	19.519808	10-11
CAGAGTA	30465	0.0	19.410116	10-11
GTACATG	21265	0.0	19.13296	1
>>END_MODULE