FastQCFastQC Report
Fri 27 May 2016
SRR2139437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9080891
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101450.047.650041
GTATCAA188450.034.003651
TCAACGC237550.029.5775554
ATCAACG240300.029.185093
CAACGCA245050.028.6370185
TATCAAC254100.027.8559232
AACGCAG253550.027.6929766
ACGCAGA292150.024.0192727
CGCAGAG296000.023.6922538
TGGTATC50850.019.9825272
GCAGAGT354050.019.9297929
TACATGG230850.019.5918452
GAGTACT220550.019.52538112-13
ACATGGG231650.018.9078043
AGAGTAC318300.018.68405510-11
GTACATG223950.018.3805891
CAGAGTA341800.018.33552710-11
GTGGTAT55500.018.15251
GTACTTT244200.017.49315314-15
AGTACTT242050.016.56746312-13
Produced by FastQC (version 0.11.5)