##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9080891
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.485769292903086	18.0	18.0	30.0	18.0	33.0
2	30.65854462959637	32.0	29.0	33.0	27.0	33.0
3	30.99655199032782	33.0	31.0	33.0	27.0	33.0
4	32.21112102325642	33.0	33.0	33.0	30.0	34.0
5	32.60278941791064	33.0	33.0	34.0	31.0	34.0
6	36.47850943260964	38.0	37.0	38.0	34.0	38.0
7	36.757561895633366	38.0	37.0	38.0	34.0	38.0
8	36.765441078414	38.0	38.0	38.0	34.0	38.0
9	37.13095950606609	38.0	38.0	38.0	36.0	38.0
10-11	37.18958348910917	38.0	38.0	38.0	36.0	38.0
12-13	37.246656853385865	38.0	38.0	38.0	36.0	38.0
14-15	37.24692846770212	38.0	38.0	38.0	36.5	38.0
16-17	37.25568542778457	38.0	38.0	38.0	36.0	38.0
18-19	37.290286052326806	38.0	38.0	38.0	37.0	38.0
20-21	37.27672216636709	38.0	38.0	38.0	37.0	38.0
22-23	37.30574385312205	38.0	38.0	38.0	37.0	38.0
24-25	37.32896930235212	38.0	38.0	38.0	37.0	38.0
26-27	37.31039099641855	38.0	38.0	38.0	37.0	38.0
28-29	37.28813090569186	38.0	38.0	38.0	37.0	38.0
30-31	37.28342575570252	38.0	38.0	38.0	37.0	38.0
32-33	37.22438421814145	38.0	38.0	38.0	36.5	38.0
34-35	37.23326412167424	38.0	38.0	38.0	37.0	38.0
36-37	37.23054300866973	38.0	38.0	38.0	37.0	38.0
38-39	37.21377393516468	38.0	38.0	38.0	37.0	38.0
40-41	37.20685845747897	38.0	38.0	38.0	37.0	38.0
42-43	37.18338019941275	38.0	38.0	38.0	36.0	38.0
44-45	37.15873258348543	38.0	38.0	38.0	36.0	38.0
46-47	37.12753939967832	38.0	38.0	38.0	36.0	38.0
48-49	37.08276786800257	38.0	38.0	38.0	36.0	38.0
50-51	37.05071678745807	38.0	38.0	38.0	36.0	38.0
52-53	37.01497705387378	38.0	38.0	38.0	36.0	38.0
54-55	36.993323400737516	38.0	38.0	38.0	36.0	38.0
56-57	36.96355693395216	38.0	38.0	38.0	36.0	38.0
58-59	36.9705819000475	38.0	38.0	38.0	36.0	38.0
60-61	36.99285726761768	38.0	38.0	38.0	36.0	38.0
62-63	37.00095977695453	38.0	38.0	38.0	36.0	38.0
64-65	36.99930903495212	38.0	38.0	38.0	36.0	38.0
66-67	37.00430472279437	38.0	38.0	38.0	36.0	38.0
68-69	37.00712859440116	38.0	38.0	38.0	36.0	38.0
70-71	36.99649064915736	38.0	38.0	38.0	36.0	38.0
72-73	36.97273619322419	38.0	38.0	38.0	36.0	38.0
74-75	36.967114482368515	38.0	38.0	38.0	36.0	38.0
76-77	36.96485518920316	38.0	38.0	38.0	36.0	38.0
78-79	36.93846418398904	38.0	38.0	38.0	36.0	38.0
80-81	36.913127541084386	38.0	38.0	38.0	35.5	38.0
82-83	36.90947387263443	38.0	38.0	38.0	35.0	38.0
84-85	36.85817950205225	38.0	38.0	38.0	35.0	38.0
86-87	36.84022601431422	38.0	38.0	38.0	35.0	38.0
88-89	36.845823519953285	38.0	38.0	38.0	35.0	38.0
90-91	36.82250271620906	38.0	38.0	38.0	35.0	38.0
92-93	36.82640400031792	38.0	38.0	38.0	35.0	38.0
94-95	36.77237341518317	38.0	38.0	38.0	35.0	38.0
96-97	36.74516555010678	38.0	38.0	38.0	35.0	38.0
98-99	36.82205297002693	38.0	38.0	38.0	35.0	38.0
100	35.204526267929005	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	31.0
19	55.0
20	127.0
21	319.0
22	848.0
23	2299.0
24	5039.0
25	9794.0
26	16989.0
27	27096.0
28	41387.0
29	60867.0
30	84142.0
31	110972.0
32	147184.0
33	198929.0
34	294014.0
35	516715.0
36	1507896.0
37	6056179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.14911895760009	18.972906953733943	19.842700457477136	24.035273631188833
2	14.325306925947935	20.915950017906123	41.781518412913904	22.977224643232034
3	17.732797365368665	25.54010393913989	31.13490735655785	25.59219133893359
4	12.323019844638592	17.933196202883618	38.7145490459031	31.0292349065747
5	13.31337843009314	37.06608960434338	35.793876421662965	13.826655543900513
6	30.0006056688401	38.001270803348135	18.587844556744013	13.410278971067749
7	26.350464948869007	32.499310915635924	23.22621205342075	17.924012082074327
8	24.117380111709302	37.057663174241384	21.0760706190615	17.748886094987814
9	25.317053139389074	16.946685077488542	21.329063414592248	36.40719836853014
10-11	24.09353883886504	26.820440857620692	28.959250804794372	20.12676949871989
12-13	24.926122337554762	24.32376404473966	29.058073706643984	21.692039911061592
14-15	22.049345157870523	25.66507515617135	26.767098074407013	25.518481611551113
16-17	21.68071392994366	28.70807501158201	27.494934142475667	22.11627691599866
18-19	21.668930945212313	27.84216879158664	29.277721756598552	21.21117850660249
20-21	22.42342764629959	27.003114228061666	29.196184034090166	21.377274091548582
22-23	22.17377509623532	26.90195946277818	29.070070677923503	21.854194763062992
24-25	21.94719152082755	27.040275540504734	29.191104736978556	21.821428201689162
26-27	21.88714978280327	27.21030357756134	29.095748301605067	21.806798338030323
28-29	21.81876215995174	27.228309692544418	29.18593900402665	21.766989143477197
30-31	22.123360330067367	27.060874227093773	29.0587214939701	21.757043948868755
32-33	21.931856850193107	27.16775512264223	29.006612686200967	21.893775340963696
34-35	21.98596369088022	27.204644937495726	29.059476528627005	21.74991484299705
36-37	22.0400637684748	27.157142432823388	28.983066044453054	21.819727754248756
38-39	21.818107782093026	27.288021810220403	29.087671786862266	21.8061986208243
40-41	22.11030490396713	27.101123285549818	28.97800185039706	21.810569960085992
42-43	22.034278560826262	27.181147219954287	28.827099197139493	21.95747502207996
44-45	22.035388033495813	27.327923841937434	28.689886190547536	21.946801934019213
46-47	22.110163054453245	27.278150540936057	28.46788006919107	22.143806335419626
48-49	22.04484005487676	27.386369813726745	28.462241360088676	22.106548771307818
50-51	22.027761482833014	27.517186237649188	28.336404437513586	22.118647842004215
52-53	22.207655274232913	27.433248575641787	28.293895666034658	22.065200484090642
54-55	22.161641639206817	27.351813025677274	28.27140289201633	22.21514244309958
56-57	22.21753080250735	27.494703364128643	28.179227198309526	22.10853863505448
58-59	22.11694725814917	27.542946009062202	28.298604280042532	22.041502452746094
60-61	22.201915103631173	27.539233701480192	28.2680976908746	21.990753504014034
62-63	22.127704345138792	27.62893680910234	28.220381569536297	22.022977276222573
64-65	22.114819394162645	27.56187406686928	28.196163739898633	22.127142799069443
66-67	22.189400499934106	27.59663913045749	28.06718052708427	22.146779842524136
68-69	22.136202842830567	27.52578484825914	28.180461776854	22.157550532056298
70-71	22.167814790654266	27.34038346788978	28.195145340650402	22.296656400805556
72-73	22.235580690865852	27.348528431977257	28.132077150906703	22.28381372625018
74-75	22.113904670847052	27.462343155166085	28.10114603627772	22.32260613770914
76-77	22.254981059680635	27.438390578792614	28.01267911929984	22.293949242226915
78-79	22.21909264843711	27.47327901761802	27.967377190608207	22.340251143336666
80-81	22.223417467114484	27.497110633274254	27.9277055836808	22.351766315930465
82-83	22.282643701703996	27.492352000927312	27.956652227946925	22.268352069421766
84-85	22.380266793963845	27.45493088014347	27.906121962822038	22.25868036307065
86-87	22.248713181386606	27.483448679245083	27.977153574919083	22.290684564449226
88-89	22.305958716237914	27.510748922537694	27.88315008831525	22.30014227290914
90-91	22.31972408162697	27.529111200624445	27.923064721169638	22.228099996578948
92-93	22.377554390583445	27.50424912966103	27.895437968912574	22.22275851084295
94-95	22.320728035477142	27.57261732532821	27.83952911165164	22.26712552754301
96-97	22.345895394165627	27.711739151682004	27.9339039008393	22.00846155331307
98-99	23.458341019003864	29.593033056730633	28.147259177912176	18.801366746353327
100	22.292445955009804	30.51626509132184	22.121636698999072	25.069652254669283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1767.0
1	2040.5
2	2907.5
3	3660.5
4	4429.0
5	5642.0
6	6764.0
7	9960.5
8	13045.0
9	13434.0
10	13155.5
11	13268.5
12	14738.5
13	17761.0
14	23825.5
15	30793.0
16	34606.5
17	35240.0
18	33578.0
19	30747.0
20	28273.0
21	27295.5
22	28701.5
23	33734.5
24	42112.0
25	53870.0
26	70125.5
27	88122.0
28	105592.5
29	125361.0
30	145895.0
31	165996.0
32	187296.5
33	208248.0
34	229457.0
35	249174.0
36	270545.0
37	291283.5
38	306063.5
39	317815.0
40	331712.0
41	344721.5
42	352981.0
43	363782.0
44	371884.5
45	375960.5
46	377519.5
47	376237.0
48	375041.5
49	369128.5
50	359728.0
51	348715.0
52	334692.5
53	317765.5
54	298612.5
55	278068.5
56	255534.5
57	230722.5
58	204741.0
59	176888.5
60	147485.0
61	120186.0
62	95442.5
63	73439.0
64	55212.5
65	40926.5
66	30382.0
67	22900.5
68	17125.5
69	12482.5
70	9153.0
71	6691.0
72	4743.5
73	3309.0
74	2284.0
75	1526.0
76	984.0
77	585.5
78	348.0
79	197.0
80	120.5
81	74.5
82	51.0
83	31.0
84	20.0
85	14.5
86	11.5
87	9.5
88	5.5
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0021914149173247427
3	0.0
4	0.0
5	0.0045810482693823765
6	2.3125484052170654E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.582817669701132E-5
44-45	2.2692559557899014E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0011848231969471483
54-55	0.002279578158186626
56-57	0.0024380322617944842
58-59	0.0016096222502733676
60-61	0.004156149666072593
62-63	0.0017968446319266622
64-65	0.0016272480553776282
66-67	2.0889423740055944E-4
68-69	1.1154077720126344E-4
70-71	1.5628506646178737E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	6.469027525323978E-5
80-81	2.415143130193904E-4
82-83	0.0
84-85	6.642977807804037E-6
86-87	0.0
88-89	0.0
90-91	4.7799097389158676E-5
92-93	2.206439742613288E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	88.0
20-21	322.0
22-23	586.0
24-25	714.0
26-27	2000.0
28-29	5002.0
30-31	13823.0
32-33	18106.0
34-35	25021.0
36-37	39826.0
38-39	46522.0
40-41	46375.0
42-43	53475.0
44-45	64503.0
46-47	65965.0
48-49	61911.0
50-51	56335.0
52-53	55358.0
54-55	59978.0
56-57	62407.0
58-59	61978.0
60-61	61274.0
62-63	59629.0
64-65	64499.0
66-67	68826.0
68-69	69923.0
70-71	72892.0
72-73	64545.0
74-75	64858.0
76-77	67618.0
78-79	69065.0
80-81	69109.0
82-83	65477.0
84-85	65141.0
86-87	67708.0
88-89	69804.0
90-91	71113.0
92-93	71311.0
94-95	87953.0
96-97	281478.0
98-99	626133.0
100-101	6202240.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.28542546464037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89816076671502	76.67002889477934
2	7.010451817866231	11.957787319721662
3	1.7257570143176255	4.415457636439986
4	0.6376791138921194	2.175389381528171
5	0.28544832007513243	1.2172290712887257
6	0.14808605086246138	0.7577749111910037
7	0.0878820923883215	0.5246543148042583
8	0.05527712730704934	0.3771466656675835
9	0.0390144918885145	0.29946307859988486
>10	0.11012257093474696	1.4068311600772003
>50	0.0017162997200575799	0.09690802853846547
>100	3.2156324653157204E-4	0.04420376735741809
>500	6.20784621091996E-5	0.03871938936558735
>1k	2.069232400745063E-5	0.018406380640712074
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1012135262938405E-5	0.0	0.0	0.0
3	0.0	1.1012135262938405E-5	0.0	0.0	0.0
4	0.0	3.3036405788815215E-5	0.0	0.0	0.0
5	0.0	6.607281157763043E-5	0.0	0.0	0.0
6	0.0	6.607281157763043E-5	0.0	0.0	1.1012135262938405E-5
7	0.0	6.607281157763043E-5	0.0	0.0	1.1012135262938405E-5
8	0.0	6.607281157763043E-5	0.0	0.0	1.1012135262938405E-5
9	0.0	1.2113348789232246E-4	0.0	2.202427052587681E-5	1.1012135262938405E-5
10-11	0.0	1.2113348789232246E-4	0.0	2.202427052587681E-5	1.1012135262938405E-5
12-13	0.0	1.2113348789232246E-4	0.0	2.7530338157346013E-5	1.1012135262938405E-5
14-15	0.0	2.422669757846449E-4	0.0	3.854247342028442E-5	1.1012135262938405E-5
16-17	0.0	2.642912463105217E-4	0.0	4.404854105175362E-5	1.1012135262938405E-5
18-19	0.0	2.8631551683639853E-4	0.0	7.157887920909963E-5	1.1012135262938405E-5
20-21	0.0	2.9732765209933694E-4	0.0	9.360314973497644E-5	1.6518202894407608E-5
22-23	0.0	3.0833978736227534E-4	0.0	1.0461528499791485E-4	2.202427052587681E-5
24-25	0.0	3.3036405788815215E-4	0.0	1.2663955552379166E-4	2.202427052587681E-5
26-27	0.0	3.5238832841402896E-4	0.0	1.6518202894407608E-4	2.202427052587681E-5
28-29	0.0	3.5789439604549814E-4	0.0	3.4137619315109056E-4	2.202427052587681E-5
30-31	1.1012135262938405E-5	3.854247342028442E-4	0.0	9.745739707700489E-4	2.202427052587681E-5
32-33	1.1012135262938405E-5	3.964368694657826E-4	0.0	0.0022519816612709036	2.202427052587681E-5
34-35	1.1012135262938405E-5	4.294732752545978E-4	0.0	0.0038267170038710956	2.202427052587681E-5
36-37	1.1012135262938405E-5	4.5700361341194384E-4	0.0	0.005814407418831478	2.202427052587681E-5
38-39	1.1012135262938405E-5	4.62509681043413E-4	0.0	0.009013432712715085	2.202427052587681E-5
40-41	1.1012135262938405E-5	5.285824926210434E-4	0.0	0.012410676441331583	2.202427052587681E-5
42-43	1.1012135262938405E-5	5.451006955154511E-4	0.0	0.01581342623757955	2.202427052587681E-5
44-45	1.1012135262938405E-5	6.166795747245507E-4	0.0	0.019706216053028275	2.202427052587681E-5
46-47	1.1012135262938405E-5	6.827523863021811E-4	0.0	0.02430378252530506	2.202427052587681E-5
48-49	1.1012135262938405E-5	6.827523863021811E-4	0.0	0.029016976417842698	2.202427052587681E-5
50-51	1.1012135262938405E-5	7.5983733314275E-4	0.0	0.033647579295908295	2.202427052587681E-5
52-53	1.1012135262938405E-5	7.928737389315652E-4	0.0	0.038267170038710954	2.202427052587681E-5
54-55	1.1012135262938405E-5	8.369222799833188E-4	0.0	0.042996882134143	2.202427052587681E-5
56-57	1.1012135262938405E-5	8.479344152462573E-4	0.0	0.04747882118615894	2.202427052587681E-5
58-59	1.1012135262938405E-5	8.699586857721341E-4	0.0	0.051900193494228704	2.202427052587681E-5
60-61	1.1012135262938405E-5	8.864768886665416E-4	0.0	0.056062780623619426	2.202427052587681E-5
62-63	1.1012135262938405E-5	8.9748902392948E-4	0.0	0.0605722500137927	2.202427052587681E-5
64-65	1.1012135262938405E-5	0.0010186225118218024	0.0	0.06496609198370512	2.202427052587681E-5
66-67	1.1012135262938405E-5	0.0010241285794532716	0.0	0.06948106744150986	2.202427052587681E-5
68-69	1.1012135262938405E-5	0.001057164985242087	0.0	0.07394648829063138	2.202427052587681E-5
70-71	1.1012135262938405E-5	0.0010736831881364944	0.0	0.07813660575817946	2.202427052587681E-5
72-73	1.1012135262938405E-5	0.0010902013910309022	0.0	0.08118146115838193	2.202427052587681E-5
74-75	1.1012135262938405E-5	0.0011232377968197174	0.0	0.08368672193070043	2.202427052587681E-5
76-77	1.1012135262938405E-5	0.0011617802702400017	0.0	0.08618097056775595	2.202427052587681E-5
78-79	1.1012135262938405E-5	0.001167286337871471	0.0	0.0877832362485135	2.202427052587681E-5
80-81	1.6518202894407608E-5	0.0011838045407658786	0.0	0.08832283087639747	2.202427052587681E-5
82-83	2.202427052587681E-5	0.0012278530818176322	0.0	0.08865319493428564	2.202427052587681E-5
84-85	2.202427052587681E-5	0.0012553834199749783	0.0	0.08886242550428146	2.202427052587681E-5
86-87	2.202427052587681E-5	0.0012663955552379166	0.0	0.08898355899217378	2.202427052587681E-5
88	2.202427052587681E-5	0.001332468366815547	0.0	0.0890000771950682	2.202427052587681E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10145	0.0	47.65004	1
GTATCAA	18845	0.0	34.00365	1
TCAACGC	23755	0.0	29.577555	4
ATCAACG	24030	0.0	29.18509	3
CAACGCA	24505	0.0	28.637018	5
TATCAAC	25410	0.0	27.855923	2
AACGCAG	25355	0.0	27.692976	6
ACGCAGA	29215	0.0	24.019272	7
CGCAGAG	29600	0.0	23.692253	8
TGGTATC	5085	0.0	19.982527	2
GCAGAGT	35405	0.0	19.929792	9
TACATGG	23085	0.0	19.591845	2
GAGTACT	22055	0.0	19.525381	12-13
ACATGGG	23165	0.0	18.907804	3
AGAGTAC	31830	0.0	18.684055	10-11
GTACATG	22395	0.0	18.380589	1
CAGAGTA	34180	0.0	18.335527	10-11
GTGGTAT	5550	0.0	18.1525	1
GTACTTT	24420	0.0	17.493153	14-15
AGTACTT	24205	0.0	16.567463	12-13
>>END_MODULE