FastQCFastQC Report
Fri 27 May 2016
SRR2139436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11342074
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111600.045.164841
GTATCAA200300.035.695041
TCAACGC241600.030.3929674
ATCAACG243850.030.0945153
CAACGCA249200.029.5013225
AACGCAG258000.028.5451666
TATCAAC266800.027.7368742
ACGCAGA300600.024.5144627
CGCAGAG301650.024.3854318
GCAGAGT371350.019.8321069
AGAGTAC329300.019.44890410-11
GAGTACT222150.017.87146412-13
TACATGG268000.017.561272
CAGAGTA367000.017.12175410-11
ACATGGG270450.016.9306773
GTACATG261350.016.9151841
AGTACTT251450.016.52297212-13
GTACTTT254250.015.64982914-15
CATGGGG202400.015.34979254
GTGGTAT61550.014.1363851
Produced by FastQC (version 0.11.5)