##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139436_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11342074
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.503879008371836	27.0	18.0	32.0	18.0	33.0
2	29.10189388642677	31.0	27.0	33.0	18.0	33.0
3	31.29476213962279	33.0	31.0	33.0	27.0	33.0
4	31.20441358432329	33.0	31.0	33.0	29.0	33.0
5	32.50537185703426	33.0	33.0	33.0	31.0	34.0
6	36.24235523414853	38.0	37.0	38.0	33.0	38.0
7	36.93274298862801	38.0	38.0	38.0	35.0	38.0
8	37.08209107082179	38.0	38.0	38.0	36.0	38.0
9	37.10892064361421	38.0	38.0	38.0	36.0	38.0
10-11	37.1961799931829	38.0	38.0	38.0	36.0	38.0
12-13	37.225927374481955	38.0	38.0	38.0	36.0	38.0
14-15	37.24763530902726	38.0	38.0	38.0	36.0	38.0
16-17	37.25879318897056	38.0	38.0	38.0	36.0	38.0
18-19	37.259908593437146	38.0	38.0	38.0	36.0	38.0
20-21	37.224172838669546	38.0	38.0	38.0	36.0	38.0
22-23	37.23616234920348	38.0	38.0	38.0	36.0	38.0
24-25	37.25466237307266	38.0	38.0	38.0	36.5	38.0
26-27	37.230497736347274	38.0	38.0	38.0	36.0	38.0
28-29	37.21009200251609	38.0	38.0	38.0	36.0	38.0
30-31	37.20741631027833	38.0	38.0	38.0	36.0	38.0
32-33	37.14637296611034	38.0	38.0	38.0	36.0	38.0
34-35	37.15950330535436	38.0	38.0	38.0	36.0	38.0
36-37	37.15568448695438	38.0	38.0	38.0	36.0	38.0
38-39	37.14701221608999	38.0	38.0	38.0	36.0	38.0
40-41	37.13738528711434	38.0	38.0	38.0	36.0	38.0
42-43	37.106953551482945	38.0	38.0	38.0	36.0	38.0
44-45	37.08264101675958	38.0	38.0	38.0	36.0	38.0
46-47	37.05636742365055	38.0	38.0	38.0	36.0	38.0
48-49	37.0130056437885	38.0	38.0	38.0	36.0	38.0
50-51	36.97997790698348	38.0	38.0	38.0	36.0	38.0
52-53	36.93790925830536	38.0	38.0	38.0	36.0	38.0
54-55	36.9060742788178	38.0	38.0	38.0	36.0	38.0
56-57	36.86706627650372	38.0	38.0	38.0	35.0	38.0
58-59	36.865894743362134	38.0	38.0	38.0	35.0	38.0
60-61	36.88604423428508	38.0	38.0	38.0	35.0	38.0
62-63	36.88969438150937	38.0	38.0	38.0	35.0	38.0
64-65	36.88150246448939	38.0	38.0	38.0	35.0	38.0
66-67	36.88027948214435	38.0	38.0	38.0	35.0	38.0
68-69	36.873044094281454	38.0	38.0	38.0	35.0	38.0
70-71	36.853271394707	38.0	38.0	38.0	35.0	38.0
72-73	36.82382468005328	38.0	38.0	38.0	35.0	38.0
74-75	36.81516499895946	38.0	38.0	38.0	35.0	38.0
76-77	36.808913748596694	38.0	38.0	38.0	35.0	38.0
78-79	36.77386138944881	38.0	38.0	38.0	35.0	38.0
80-81	36.738970160406645	38.0	38.0	38.0	35.0	38.0
82-83	36.73386143502493	38.0	38.0	38.0	35.0	38.0
84-85	36.678475042785934	38.0	38.0	38.0	34.5	38.0
86-87	36.65457365973826	38.0	38.0	38.0	34.0	38.0
88-89	36.65256581980594	38.0	38.0	38.0	34.0	38.0
90-91	36.61842917307681	38.0	38.0	38.0	34.0	38.0
92-93	36.61976393431872	38.0	38.0	38.0	34.0	38.0
94-95	36.56127583510455	38.0	38.0	38.0	34.0	38.0
96-97	36.52561878077092	38.0	38.0	38.0	34.0	38.0
98-99	36.612116411594855	38.0	38.0	38.0	34.0	38.0
100	34.75931319522312	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	36.0
19	75.0
20	211.0
21	476.0
22	1365.0
23	3307.0
24	7238.0
25	14148.0
26	24164.0
27	38786.0
28	58346.0
29	83383.0
30	113586.0
31	151545.0
32	199192.0
33	273196.0
34	405065.0
35	714888.0
36	2019653.0
37	7233409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.859845915306146	24.124194569705683	14.631556803455876	24.3844027115323
2	13.403702720448635	22.84425824578159	40.960638503570294	22.791400530199475
3	17.314505265968112	27.066443050891753	30.848811249159546	24.770240433980593
4	12.599723824760797	19.048720718979613	39.3265993503481	29.024956105911492
5	13.376943687818235	38.12351272729389	35.4458333175361	13.053710267351775
6	29.135307338098315	38.84131911568391	19.2216435595919	12.80172998662588
7	25.520561759692274	33.00231509686853	24.27579823584293	17.201324907596266
8	23.707401309495953	37.18914195058152	21.83225924993965	17.271197489982875
9	25.18169075602928	16.898514328155503	21.885362412553473	36.03443250326175
10-11	23.51204462252671	27.226356484713467	29.625604629276797	19.635994263483028
12-13	24.391165143165175	24.77813140700722	29.65362860443337	21.177074845394237
14-15	21.721287482342294	26.151747026161175	27.14598317732718	24.98098231416935
16-17	21.409069452377054	28.99732006685902	27.93156260486398	21.662047875899944
18-19	21.4425113078966	28.378491446978742	29.354587176913142	20.82441006821151
20-21	22.21981277906638	27.564274124880228	29.123574236258808	21.092338859794584
22-23	21.955589464191434	27.47991286581738	28.94909425382054	21.615403416170643
24-25	21.750217142812804	27.582024344595958	29.005438963989054	21.662319548602184
26-27	21.681755370367046	27.736185636314794	28.937595679941385	21.64446331337678
28-29	21.667701351434424	27.753085378823833	28.99876411101146	21.580449158730286
30-31	21.90543875797062	27.601130778054696	28.915320337923994	21.578110126050685
32-33	21.779632894318333	27.669741264415848	28.87213254167012	21.678493299595697
34-35	21.889241450347765	27.70764215107749	28.843718564406302	21.559397834168443
36-37	21.819364866342035	27.6812423696557	28.848847395241705	21.65054536876056
38-39	21.7030992963937	27.791848401306023	28.925252472772833	21.579799829527442
40-41	21.93998448083148	27.647514376575653	28.806660605286865	21.605840537305998
42-43	21.83860690807961	27.72119510179838	28.628137960013433	21.81206003010858
44-45	21.903952600707974	27.755204819801477	28.537509759325758	21.803332820164794
46-47	21.918286516735552	27.782528118484883	28.39932872995637	21.899856634823195
48-49	21.881223493430042	27.859769793383947	28.3960143024667	21.86299241071931
50-51	21.926414241405627	27.955645910551763	28.270109402813098	21.847830445229512
52-53	22.067661109629892	27.859576961588782	28.220386042365924	21.8523758864154
54-55	21.996316064379705	27.801625816920083	28.25762234206119	21.94443577663902
56-57	22.028345323653912	27.864731471591746	28.22910895373757	21.877814251016765
58-59	21.99674505346726	27.891205437995005	28.249319817350145	21.862729691187592
60-61	22.064300707284783	27.84863986848542	28.286278867413245	21.800780556816544
62-63	22.03751168475174	27.910625861915584	28.263356898184988	21.78850555514769
64-65	22.027992833861994	27.816631814055746	28.221355354449095	21.934019997633154
66-67	22.04989337494514	27.848957397249656	28.14021284698135	21.960936380823856
68-69	21.99937401259233	27.840831031385534	28.22303901374461	21.936755942277525
70-71	22.00957762056774	27.728917696563084	28.238262266174075	22.023242416695112
72-73	22.057935246789555	27.73732739058832	28.163638771800247	22.041098590821875
74-75	21.994705625025503	27.80808366803676	28.12154082030004	22.0756698866377
76-77	22.103675359637652	27.746460194382294	28.0802707572357	22.06959368874436
78-79	22.04030917152483	27.759766097201393	28.069131876826237	22.130792854447538
80-81	22.034029483056372	27.84651026989817	28.035527636398477	22.08393261064698
82-83	22.082173607196214	27.813280096885485	28.087160940086477	22.01738535583183
84-85	22.1585396961337	27.78454811899364	28.00452900313783	22.05238318173483
86-87	22.079909182638065	27.787887119766946	28.053667469982997	22.078536227611995
88-89	22.104847539587546	27.794783213562173	28.00002268701935	22.100346559830925
90-91	22.152287709695965	27.82091881166672	27.97600194281581	22.050791535821507
92-93	22.14809658224692	27.802441141055105	28.013468897355096	22.035993379342873
94-95	22.152738790287376	27.849026832172534	27.938344356854255	22.05989002068583
96-97	22.188760193641603	27.941115354595546	28.01542283987994	21.854701611882913
98-99	23.22329577882563	29.857695534842048	28.316973350218568	18.60203533611375
100	22.030679633103986	30.80890365105257	22.29591906724503	24.86449764859841
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1377.0
1	1635.5
2	2659.5
3	3727.5
4	4655.0
5	6022.5
6	7205.0
7	9354.5
8	11588.0
9	12505.0
10	12629.0
11	12748.5
12	13990.5
13	16793.0
14	22956.0
15	30169.0
16	34254.0
17	34827.0
18	33354.0
19	31268.0
20	29429.0
21	29096.5
22	32059.5
23	39314.5
24	50729.5
25	66689.0
26	87044.0
27	110344.5
28	136145.5
29	164571.0
30	193131.5
31	222151.0
32	252215.5
33	281045.0
34	307773.0
35	332165.0
36	356983.5
37	381296.5
38	402188.5
39	418891.5
40	435288.5
41	449093.0
42	457002.0
43	464022.0
44	469411.5
45	471921.5
46	470191.5
47	467197.0
48	462128.5
49	449512.0
50	432573.5
51	412441.5
52	389604.5
53	364620.0
54	338431.5
55	310621.5
56	281657.0
57	251642.0
58	219982.0
59	187234.5
60	153878.0
61	123282.5
62	96326.5
63	73106.5
64	53747.5
65	39071.0
66	28628.0
67	21479.0
68	16031.5
69	11618.0
70	8489.0
71	6192.0
72	4388.0
73	2953.5
74	1933.5
75	1263.0
76	798.0
77	432.0
78	231.5
79	135.0
80	81.5
81	47.0
82	28.5
83	18.5
84	9.0
85	4.5
86	4.0
87	4.0
88	4.5
89	3.5
90	3.5
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0026891025398000402
3	0.0
4	0.0
5	0.003747110096442679
6	1.8515132241246176E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.17361497782658E-5
44-45	2.7032576011210952E-5
46-47	0.0
48-49	0.0
50-51	4.582412535721051E-6
52-53	0.0010270432934211106
54-55	0.0020231328809879813
56-57	0.0024956738936447154
58-59	0.0017377524518703462
60-61	0.0038071734498278153
62-63	0.0016777702035879352
64-65	0.0016115635885802124
66-67	2.2076028787333505E-4
68-69	1.8846273651952625E-4
70-71	2.287193550737082E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.101443496774698E-4
80-81	3.077627554796654E-4
82-83	5.083823608809128E-6
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.1484688838394445E-5
92-93	2.4875300647117716E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	71.0
20-21	293.0
22-23	528.0
24-25	712.0
26-27	1409.0
28-29	3302.0
30-31	10933.0
32-33	14711.0
34-35	21943.0
36-37	36735.0
38-39	43612.0
40-41	43983.0
42-43	51337.0
44-45	62349.0
46-47	64471.0
48-49	60368.0
50-51	55361.0
52-53	55222.0
54-55	60242.0
56-57	63852.0
58-59	63255.0
60-61	62321.0
62-63	62168.0
64-65	66755.0
66-67	71274.0
68-69	72242.0
70-71	71641.0
72-73	68773.0
74-75	71038.0
76-77	75141.0
78-79	76535.0
80-81	75845.0
82-83	73860.0
84-85	73690.0
86-87	77235.0
88-89	79957.0
90-91	81334.0
92-93	83143.0
94-95	106271.0
96-97	359402.0
98-99	827203.0
100-101	8091557.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.69348152981581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65473944823029	75.93172019519142
2	7.1399432271035845	12.09413299657262
3	1.7610658846069196	4.474524029122346
4	0.6451924463657513	2.1857437815781777
5	0.29708878238645436	1.258074165188132
6	0.16071847896165756	0.816708451766354
7	0.09584305839772107	0.5682097607317914
8	0.06409157902509048	0.43425111715025844
9	0.04590943105176234	0.3499406595744108
>10	0.13277451776072188	1.706932684459885
>50	0.0022817222960885546	0.12316986245486639
>100	3.1674689908820825E-4	0.039441007717107374
>500	3.4676914813073606E-5	0.01715128849265362
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.816729638688656E-6
2	0.0	8.816729638688656E-6	0.0	8.816729638688656E-6	1.763345927737731E-5
3	0.0	1.763345927737731E-5	0.0	1.763345927737731E-5	1.763345927737731E-5
4	0.0	4.408364819344328E-5	0.0	3.526691855475462E-5	1.763345927737731E-5
5	0.0	4.408364819344328E-5	0.0	3.526691855475462E-5	1.763345927737731E-5
6	0.0	4.408364819344328E-5	0.0	3.526691855475462E-5	1.763345927737731E-5
7	0.0	4.408364819344328E-5	0.0	4.408364819344328E-5	1.763345927737731E-5
8	0.0	5.290037783213193E-5	0.0	4.408364819344328E-5	2.6450188916065967E-5
9	0.0	7.935056674819791E-5	0.0	4.408364819344328E-5	2.6450188916065967E-5
10-11	0.0	7.935056674819791E-5	0.0	5.730874265147626E-5	2.6450188916065967E-5
12-13	8.816729638688656E-6	7.935056674819791E-5	0.0	7.053383710950924E-5	2.6450188916065967E-5
14-15	8.816729638688656E-6	8.816729638688656E-5	0.0	7.053383710950924E-5	3.526691855475462E-5
16-17	8.816729638688656E-6	8.816729638688656E-5	0.0	7.494220192885358E-5	3.526691855475462E-5
18-19	8.816729638688656E-6	8.816729638688656E-5	0.0	1.0139239084491953E-4	3.526691855475462E-5
20-21	8.816729638688656E-6	8.816729638688656E-5	0.0	1.0580075566426387E-4	3.526691855475462E-5
22-23	8.816729638688656E-6	1.0580075566426387E-4	0.0	1.1461748530295253E-4	3.9675283374098955E-5
24-25	8.816729638688656E-6	1.1461748530295252E-4	0.0	1.278425797609855E-4	6.171710747082059E-5
26-27	8.816729638688656E-6	1.2343421494164118E-4	0.0	1.3225094458032985E-4	6.171710747082059E-5
28-29	8.816729638688656E-6	1.2343421494164118E-4	0.0	1.719262279544288E-4	6.171710747082059E-5
30-31	8.816729638688656E-6	1.4547603903836283E-4	0.0	4.0116119856033384E-4	6.171710747082059E-5
32-33	8.816729638688656E-6	1.5429276867705147E-4	0.0	0.0010844577455587047	7.053383710950924E-5
34-35	8.816729638688656E-6	1.895596872318061E-4	0.0	0.0020498896409951125	7.053383710950924E-5
36-37	8.816729638688656E-6	2.204182409672164E-4	0.0	0.00357518386848825	7.053383710950924E-5
38-39	8.816729638688656E-6	2.204182409672164E-4	0.0	0.006158485652624026	7.935056674819791E-5
40-41	8.816729638688656E-6	2.380517002445937E-4	0.0	0.00902392278519784	7.935056674819791E-5
42-43	8.816729638688656E-6	2.55685159521971E-4	0.0	0.01161163293415296	7.935056674819791E-5
44-45	8.816729638688656E-6	3.041771725347586E-4	0.0	0.014900273089383829	7.935056674819791E-5
46-47	8.816729638688656E-6	3.262189966314803E-4	0.0	0.01885457633233569	7.935056674819791E-5
48-49	8.816729638688656E-6	3.262189966314803E-4	0.0	0.022698670454803945	7.935056674819791E-5
50-51	8.816729638688656E-6	3.5266918554754625E-4	0.0	0.02651190602353679	7.935056674819791E-5
52-53	8.816729638688656E-6	3.5266918554754625E-4	0.0	0.030404492159017832	7.935056674819791E-5
54-55	8.816729638688656E-6	3.614859151862349E-4	0.0	0.034305895024137556	7.935056674819791E-5
56-57	8.816729638688656E-6	3.703026448249236E-4	0.0	0.03826019826708943	7.935056674819791E-5
58-59	8.816729638688656E-6	3.703026448249236E-4	0.0	0.04229826044160882	8.375893156754223E-5
60-61	8.816729638688656E-6	3.791193744636122E-4	0.0	0.04645975683106987	8.816729638688656E-5
62-63	8.816729638688656E-6	4.055695633796782E-4	0.0	0.05033911787209288	8.816729638688656E-5
64-65	8.816729638688656E-6	4.4965321157312143E-4	0.0	0.05430223784468344	8.816729638688656E-5
66-67	8.816729638688656E-6	4.4965321157312143E-4	0.0	0.05843728404522841	9.25756612062309E-5
68-69	8.816729638688656E-6	5.025535894052533E-4	0.0	0.06266931427179898	1.0580075566426387E-4
70-71	8.816729638688656E-6	5.11370319043942E-4	0.0	0.06616514757353903	1.0580075566426387E-4
72-73	8.816729638688656E-6	5.11370319043942E-4	0.0	0.06876608281695218	1.410676742190185E-4
74-75	8.816729638688656E-6	5.422288727793523E-4	0.0	0.07085564774132139	1.410676742190185E-4
76-77	8.816729638688656E-6	5.64270696876074E-4	0.0	0.07309509706954831	1.410676742190185E-4
78-79	8.816729638688656E-6	5.730874265147626E-4	0.0	0.0744264232449903	1.410676742190185E-4
80-81	8.816729638688656E-6	5.730874265147626E-4	0.0	0.07491134337511816	1.4547603903836283E-4
82-83	8.816729638688656E-6	5.730874265147626E-4	0.0	0.07529046274958179	1.4988440385770715E-4
84-85	8.816729638688656E-6	5.730874265147626E-4	0.0	0.07552851444982638	1.4988440385770715E-4
86-87	8.816729638688656E-6	5.863125209727956E-4	0.0	0.07564754029994866	1.5429276867705147E-4
88	8.816729638688656E-6	6.259878043468946E-4	0.0	0.07565635702958735	1.5870113349639582E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11160	0.0	45.16484	1
GTATCAA	20030	0.0	35.69504	1
TCAACGC	24160	0.0	30.392967	4
ATCAACG	24385	0.0	30.094515	3
CAACGCA	24920	0.0	29.501322	5
AACGCAG	25800	0.0	28.545166	6
TATCAAC	26680	0.0	27.736874	2
ACGCAGA	30060	0.0	24.514462	7
CGCAGAG	30165	0.0	24.385431	8
GCAGAGT	37135	0.0	19.832106	9
AGAGTAC	32930	0.0	19.448904	10-11
GAGTACT	22215	0.0	17.871464	12-13
TACATGG	26800	0.0	17.56127	2
CAGAGTA	36700	0.0	17.121754	10-11
ACATGGG	27045	0.0	16.930677	3
GTACATG	26135	0.0	16.915184	1
AGTACTT	25145	0.0	16.522972	12-13
GTACTTT	25425	0.0	15.649829	14-15
CATGGGG	20240	0.0	15.3497925	4
GTGGTAT	6155	0.0	14.136385	1
>>END_MODULE