FastQCFastQC Report
Fri 27 May 2016
SRR2139435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6676806
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76200.042.3916931
GTATCAA131350.034.4831961
TCAACGC152450.029.3355924
ATCAACG153500.029.1063393
CAACGCA156700.028.5679665
AACGCAG162900.027.5863636
TATCAAC169150.026.906962
ACGCAGA188200.023.8079367
CGCAGAG190800.023.4835078
GTACATG173900.019.9634111
TACATGG172800.019.93812
ACATGGG174300.019.136583
GCAGAGT234200.019.0942619
AGAGTAC205650.018.8962410-11
GAGTACT133750.017.86568512-13
CATGGGG121450.017.4179734
CAGAGTA225650.016.95886210-11
AGTACTT149250.015.98088412-13
GTACTTT153100.015.56491514-15
GTGGTAT42950.014.7148951
Produced by FastQC (version 0.11.5)