##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139435_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6676806
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.07175511764158	30.0	18.0	33.0	18.0	33.0
2	29.977668813501545	32.0	28.0	33.0	25.0	33.0
3	31.70659623778196	33.0	31.0	33.0	29.0	33.0
4	31.544522036434785	33.0	31.0	33.0	29.0	33.0
5	32.64939972795376	33.0	33.0	33.0	31.0	34.0
6	36.404214380348925	38.0	37.0	38.0	34.0	38.0
7	37.02407273777312	38.0	38.0	38.0	36.0	38.0
8	37.18571409742922	38.0	38.0	38.0	36.0	38.0
9	37.22205467704169	38.0	38.0	38.0	36.0	38.0
10-11	37.28134769828567	38.0	38.0	38.0	36.5	38.0
12-13	37.2999300863317	38.0	38.0	38.0	37.0	38.0
14-15	37.320985213588656	38.0	38.0	38.0	37.0	38.0
16-17	37.332350752740155	38.0	38.0	38.0	37.0	38.0
18-19	37.333996749343925	38.0	38.0	38.0	37.0	38.0
20-21	37.29690705457189	38.0	38.0	38.0	37.0	38.0
22-23	37.30940246307084	38.0	38.0	38.0	37.0	38.0
24-25	37.32993548635018	38.0	38.0	38.0	37.0	38.0
26-27	37.304507031744706	38.0	38.0	38.0	37.0	38.0
28-29	37.28243186187434	38.0	38.0	38.0	37.0	38.0
30-31	37.28141319575914	38.0	38.0	38.0	37.0	38.0
32-33	37.21998952715896	38.0	38.0	38.0	36.5	38.0
34-35	37.23091547229647	38.0	38.0	38.0	37.0	38.0
36-37	37.2262765050077	38.0	38.0	38.0	37.0	38.0
38-39	37.21826264833412	38.0	38.0	38.0	37.0	38.0
40-41	37.21197008055158	38.0	38.0	38.0	37.0	38.0
42-43	37.18774815247527	38.0	38.0	38.0	36.0	38.0
44-45	37.1682022735455	38.0	38.0	38.0	36.0	38.0
46-47	37.14250604360467	38.0	38.0	38.0	36.0	38.0
48-49	37.102508183619385	38.0	38.0	38.0	36.0	38.0
50-51	37.07555959552606	38.0	38.0	38.0	36.0	38.0
52-53	37.043871734869995	38.0	38.0	38.0	36.0	38.0
54-55	37.01872078924508	38.0	38.0	38.0	36.0	38.0
56-57	36.98531097577633	38.0	38.0	38.0	36.0	38.0
58-59	36.99087727223581	38.0	38.0	38.0	36.0	38.0
60-61	37.01685637510246	38.0	38.0	38.0	36.0	38.0
62-63	37.02361407900824	38.0	38.0	38.0	36.0	38.0
64-65	37.02206493048136	38.0	38.0	38.0	36.0	38.0
66-67	37.025323928350744	38.0	38.0	38.0	36.0	38.0
68-69	37.0230841530695	38.0	38.0	38.0	36.0	38.0
70-71	37.01135517288691	38.0	38.0	38.0	36.0	38.0
72-73	36.990927698950415	38.0	38.0	38.0	36.0	38.0
74-75	36.98651566552011	38.0	38.0	38.0	36.0	38.0
76-77	36.98212698890963	38.0	38.0	38.0	36.0	38.0
78-79	36.95837972956991	38.0	38.0	38.0	36.0	38.0
80-81	36.93155562506719	38.0	38.0	38.0	36.0	38.0
82-83	36.93082759967749	38.0	38.0	38.0	36.0	38.0
84-85	36.880772327103486	38.0	38.0	38.0	35.5	38.0
86-87	36.866046786349074	38.0	38.0	38.0	35.0	38.0
88-89	36.869675248446235	38.0	38.0	38.0	35.0	38.0
90-91	36.84758946813375	38.0	38.0	38.0	35.0	38.0
92-93	36.85224088102848	38.0	38.0	38.0	35.0	38.0
94-95	36.79998100820218	38.0	38.0	38.0	35.0	38.0
96-97	36.77374299900892	38.0	38.0	38.0	35.0	38.0
98-99	36.84842132540733	38.0	38.0	38.0	35.0	38.0
100	35.24482771963694	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	41.0
20	94.0
21	217.0
22	586.0
23	1514.0
24	3571.0
25	7067.0
26	12104.0
27	19648.0
28	29540.0
29	43610.0
30	59243.0
31	77718.0
32	102574.0
33	138682.0
34	204267.0
35	357258.0
36	1012093.0
37	4606970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.10064273246819	22.727903132126347	14.814403773301185	24.357050362104278
2	13.453172305892227	22.452972504375712	41.51333323148548	22.580521958246575
3	17.17929201477473	27.181709338267428	31.039811550612672	24.59918709634517
4	12.292509322571302	19.111982585685432	39.76212578289679	28.833382308846478
5	13.116937699170398	38.212377726825835	35.74868597714592	12.921998596857847
6	28.677921068416808	39.04591948681965	19.49057893794852	12.785580506815023
7	25.264535168462288	33.13894398010066	24.514565796879527	17.081955054557525
8	23.625517949750225	37.17988810817627	22.0302342167797	17.164359725293803
9	25.104713241630805	17.038371341027432	22.089768670828537	35.76714674651323
10-11	23.42176034469176	27.308409140538153	29.7468654922728	19.522965022497285
12-13	24.33345375019133	24.877059480236507	29.813461706091203	20.976025063480954
14-15	21.618143465603165	26.240368523512593	27.325535592916733	24.815952417967512
16-17	21.19619021430307	29.12315409493701	28.114984919436033	21.565670771323894
18-19	21.253066211598778	28.514652065673317	29.526101252604914	20.70618047012299
20-21	22.115246289568766	27.652389094076423	29.28325118301624	20.949113433338574
22-23	21.808850555094686	27.597343681650404	29.117455580554953	21.47635018269996
24-25	21.611119707405763	27.684349452808814	29.159086657091077	21.545444182694347
26-27	21.561331245402503	27.911177103586944	29.076901033082347	21.45059061792821
28-29	21.497031283722855	27.8909727457335	29.1949949579356	21.417001012608043
30-31	21.78247334031512	27.715406978552558	29.10858120282826	21.393538478304063
32-33	21.601505657657956	27.835587105574227	29.03122243901085	21.53168479775697
34-35	21.73192103549006	27.86328775742628	29.001850141332223	21.40294106575144
36-37	21.696451414197792	27.815635642464336	29.019735905149325	21.46817703818855
38-39	21.525745787738973	27.908136276434643	29.12595097133816	21.440166964488228
40-41	21.77292629565596	27.772264959839553	28.990075865446236	21.46473287905825
42-43	21.680428678110943	27.83991096582244	28.86265664442996	21.617003711636656
44-45	21.765170294732254	27.878727372090033	28.741918753972577	21.614183579205132
46-47	21.77577390128682	27.862271500757718	28.55976571217956	21.80218888577591
48-49	21.73672910838286	27.95803906756651	28.59478562042373	21.7104462036269
50-51	21.74739854038795	28.038998176925894	28.470496436024867	21.743106846661288
52-53	21.876459124777227	27.97178406941856	28.431791015283093	21.71996579052112
54-55	21.85822643465693	27.90833224528106	28.44108041505121	21.792360905010792
56-57	21.877108019072757	27.97221883171357	28.386172233934648	21.764500915279026
58-59	21.825536025666132	28.02626242468581	28.444600030212875	21.703601519435185
60-61	21.873247515678226	28.013527977260566	28.446718067532533	21.666506439528675
62-63	21.84763033564913	28.072888747345935	28.388346516490735	21.691134400514198
64-65	21.8406263435305	27.989408307851438	28.35333659300569	21.816628755612374
66-67	21.913295641084314	27.99418197581068	28.275870116788088	21.816652266316925
68-69	21.836296654988242	27.99998981078714	28.340991540241678	21.82272199398294
70-71	21.8609922434175	27.830556780782413	28.379153139748407	21.92929783605168
72-73	21.926084446867634	27.847044416304783	28.327139145130353	21.899731991697227
74-75	21.831631993797647	27.952997383802398	28.256150749366927	21.959219873033028
76-77	21.95258816417712	27.90695178797369	28.183351272133887	21.95710877571531
78-79	21.9216374711075	27.92809427298495	28.178771212238846	21.971497043668712
80-81	21.90494629728979	27.929441108535524	28.180201874769566	21.985410719405117
82-83	21.934398876776452	27.93572580732989	28.222443761464355	21.907431554429305
84-85	22.0808502438544	27.865072601660128	28.13912853278897	21.9149486216965
86-87	21.933814834087013	27.92413972382973	28.168460867162835	21.973584574920427
88-89	21.99523116572212	27.90835398460475	28.141463390477757	21.954951459195374
90-91	22.00151203151189	27.908083536619948	28.168515715817975	21.921888716050184
92-93	22.044751707603496	27.9236957026335	28.10976470140692	21.921787888356082
94-95	21.997506665350055	27.966843150808263	28.1025280409389	21.933122142902782
96-97	22.010322722671944	28.12671352834018	28.167309022175946	21.69565472681193
98-99	23.095431566830722	30.00794133232884	28.45021234906435	18.44641475177609
100	21.924591326318783	30.98779234116605	22.431282897041736	24.656333435473428
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	641.0
1	767.5
2	1408.5
3	2159.0
4	2802.0
5	3728.5
6	4495.0
7	5613.0
8	6853.5
9	7368.0
10	7444.5
11	7527.0
12	8249.0
13	10112.5
14	13836.0
15	18029.0
16	20495.0
17	21182.5
18	20578.0
19	19113.5
20	17806.5
21	17630.5
22	19566.5
23	23997.5
24	31070.5
25	41284.0
26	54592.0
27	69332.0
28	84364.0
29	100648.5
30	117227.5
31	134048.5
32	151651.5
33	168330.5
34	185366.0
35	201098.0
36	216102.5
37	231150.5
38	242274.5
39	250542.5
40	260113.5
41	268007.5
42	272635.5
43	277272.5
44	280070.5
45	281001.0
46	279208.5
47	275181.0
48	269610.5
49	261126.5
50	250949.5
51	238558.5
52	224108.0
53	208747.0
54	192054.5
55	174715.5
56	157622.0
57	140346.5
58	123124.0
59	105699.0
60	86773.0
61	68720.5
62	53439.0
63	40535.0
64	29886.5
65	21725.5
66	15856.0
67	11686.0
68	8562.5
69	6101.0
70	4438.0
71	3204.0
72	2226.0
73	1496.0
74	974.0
75	627.0
76	405.0
77	231.5
78	129.5
79	67.5
80	40.5
81	29.0
82	19.5
83	14.0
84	9.0
85	6.5
86	5.0
87	5.5
88	5.5
89	4.0
90	2.5
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0026958998059850776
3	0.0
4	0.0
5	0.005421754054258878
6	1.6474943258797695E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.380469027424475E-5
44-45	4.593681238333964E-5
46-47	0.0
48-49	0.0
50-51	1.55778332417876E-5
52-53	8.690084120797179E-4
54-55	0.002266560232775736
56-57	0.0024458557586249066
58-59	0.0017120865987735408
60-61	0.003933421513540544
62-63	0.001740604202398746
64-65	0.001816000701105985
66-67	2.3665044631866156E-4
68-69	1.9721018258622583E-4
70-71	1.986104879247306E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	9.364090568462442E-5
80-81	3.25974208234383E-4
82-83	8.643371800083305E-6
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.6752142735165827E-5
92-93	2.518085474432934E-4
94-95	0.0
96-97	0.0
98-99	9.917986188609154E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	66.0
20-21	254.0
22-23	394.0
24-25	430.0
26-27	912.0
28-29	2103.0
30-31	6436.0
32-33	9013.0
34-35	13265.0
36-37	21844.0
38-39	26057.0
40-41	26147.0
42-43	30201.0
44-45	37400.0
46-47	38269.0
48-49	36281.0
50-51	33102.0
52-53	32674.0
54-55	35690.0
56-57	37934.0
58-59	37670.0
60-61	36871.0
62-63	36858.0
64-65	39356.0
66-67	41990.0
68-69	42733.0
70-71	43279.0
72-73	40305.0
74-75	41438.0
76-77	43110.0
78-79	44748.0
80-81	44528.0
82-83	42715.0
84-85	42744.0
86-87	44292.0
88-89	46653.0
90-91	47407.0
92-93	48017.0
94-95	61560.0
96-97	206974.0
98-99	474071.0
100-101	4781015.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.30311067178243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.63160703453686	79.12421219297666
2	6.857745700791254	11.974050637502382
3	1.5021723471156394	3.9343295600498354
4	0.5241180258403916	1.8302853606007932
5	0.19799399612840224	0.8642745878173183
6	0.10329937510113207	0.541101406606806
7	0.06069800407271711	0.3709387196981706
8	0.03665223246203643	0.2559883125600851
9	0.020388383404792768	0.160197236352662
>10	0.06431210370951942	0.8142075267049245
>50	6.09443130203948E-4	0.036717473879266695
>100	3.544355084324618E-4	0.06531811127806642
>500	4.891819862812981E-5	0.028378873973110036
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.497722114436154E-5	0.0	0.0	0.0
3	0.0	1.497722114436154E-5	0.0	0.0	0.0
4	0.0	4.493166343308462E-5	0.0	0.0	0.0
5	1.497722114436154E-5	4.493166343308462E-5	0.0	0.0	0.0
6	4.493166343308462E-5	4.493166343308462E-5	0.0	0.0	0.0
7	4.493166343308462E-5	4.493166343308462E-5	0.0	0.0	0.0
8	4.493166343308462E-5	4.493166343308462E-5	0.0	0.0	0.0
9	4.493166343308462E-5	5.990888457744616E-5	0.0	0.0	1.497722114436154E-5
10-11	7.48861057218077E-5	5.990888457744616E-5	0.0	1.497722114436154E-5	2.246583171654231E-5
12-13	8.986332686616924E-5	6.739749514962694E-5	0.0	2.995444228872308E-5	3.7443052860903846E-5
14-15	9.735193743835E-5	1.1232915858271155E-4	0.0	2.995444228872308E-5	6.739749514962693E-5
16-17	1.0484054801053079E-4	1.1981776915489232E-4	0.0	3.7443052860903846E-5	8.986332686616924E-5
18-19	1.0484054801053079E-4	1.3479499029925388E-4	0.0	4.493166343308462E-5	8.986332686616924E-5
20-21	1.0484054801053079E-4	1.572608220157962E-4	0.0	4.493166343308462E-5	8.986332686616924E-5
22-23	1.2730637972707307E-4	1.722380431601577E-4	0.0	5.990888457744616E-5	1.0484054801053079E-4
24-25	1.572608220157962E-4	2.2465831716542313E-4	0.0	7.48861057218077E-5	1.0484054801053079E-4
26-27	1.6474943258797695E-4	2.546127594541462E-4	0.0	8.237471629398847E-5	1.0484054801053079E-4
28-29	1.6474943258797695E-4	2.546127594541462E-4	0.0	2.6958998059850776E-4	1.1981776915489232E-4
30-31	1.6474943258797695E-4	2.8456720174286926E-4	0.0	6.889521726406308E-4	1.3479499029925385E-4
32-33	1.7972665373233848E-4	2.8456720174286926E-4	0.0	0.001887129864189554	1.497722114436154E-4
34-35	1.8721526430451926E-4	2.9954442288723077E-4	0.0	0.003609510295791131	1.722380431601577E-4
36-37	1.9470387487670004E-4	3.2201025460377314E-4	0.0	0.0064327164815032815	1.947038748767E-4
38-39	1.9470387487670004E-4	3.3698747574813464E-4	0.0	0.010633827012496693	2.0968109602106157E-4
40-41	2.246583171654231E-4	3.5945330746467696E-4	0.0	0.015291742788393133	2.1716970659324233E-4
42-43	2.3963553830978464E-4	3.744305286090385E-4	0.0	0.019942169953717392	2.3963553830978464E-4
44-45	2.6210137002632695E-4	4.3433941318648465E-4	0.0	0.024952050426506327	2.6210137002632695E-4
46-47	2.8456720174286926E-4	4.942482977639309E-4	0.0	0.030898007220817855	2.770785911706885E-4
48-49	2.8456720174286926E-4	4.942482977639309E-4	0.0	0.03722588315431061	3.294988651759539E-4
50-51	2.8456720174286926E-4	5.242027400526539E-4	0.0	0.04374097435210788	3.294988651759539E-4
52-53	3.070330334594116E-4	5.242027400526539E-4	0.0	0.050128759170178075	3.294988651759539E-4
54-55	3.1452164403159233E-4	5.391799611970155E-4	0.0	0.05555051322443695	3.3698747574813464E-4
56-57	3.294988651759539E-4	5.54157182341377E-4	0.0	0.05943710211139877	3.4447608632031545E-4
58-59	3.5945330746467696E-4	5.54157182341377E-4	0.0	0.06381045068555234	3.6694191803685776E-4
60-61	3.744305286090385E-4	5.54157182341377E-4	0.0	0.06738251792848257	3.744305286090385E-4
62-63	3.744305286090385E-4	5.691344034857385E-4	0.0	0.07081230157054136	3.894077497534001E-4
64-65	3.744305286090385E-4	6.290432880631847E-4	0.0	0.07468391323635883	3.9689636032558083E-4
66-67	3.744305286090385E-4	6.664863409240885E-4	0.0	0.07898237570479058	4.043849708977616E-4
68-69	3.894077497534001E-4	7.263952255015347E-4	0.0	0.08254695433714863	4.043849708977616E-4
70-71	4.043849708977616E-4	7.338838360737155E-4	0.0	0.0857970113254751	4.043849708977616E-4
72-73	4.043849708977616E-4	7.338838360737155E-4	0.0	0.08881492138606394	4.1936219204212314E-4
74-75	4.268508026143039E-4	7.638382783624385E-4	0.0	0.09091173234627456	4.268508026143039E-4
76-77	4.56805244903027E-4	8.162585523677039E-4	0.0	0.09300854330648517	4.418280237586654E-4
78-79	4.6429385547520777E-4	8.237471629398848E-4	0.0	0.0943564932094777	4.493166343308462E-4
80-81	4.717824660473885E-4	8.237471629398848E-4	0.0	0.09473092373808674	4.493166343308462E-4
82-83	4.867596871917501E-4	8.237471629398848E-4	0.0	0.09508288843497924	4.6429385547520777E-4
84-85	5.242027400526539E-4	8.387243840842463E-4	0.0	0.0952176834252785	4.6429385547520777E-4
86-87	5.242027400526539E-4	8.537016052286078E-4	0.0	0.09528508092042812	4.6429385547520777E-4
88	5.242027400526539E-4	8.986332686616924E-4	0.0	0.09530005814157248	4.792710766195693E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7620	0.0	42.391693	1
GTATCAA	13135	0.0	34.483196	1
TCAACGC	15245	0.0	29.335592	4
ATCAACG	15350	0.0	29.106339	3
CAACGCA	15670	0.0	28.567966	5
AACGCAG	16290	0.0	27.586363	6
TATCAAC	16915	0.0	26.90696	2
ACGCAGA	18820	0.0	23.807936	7
CGCAGAG	19080	0.0	23.483507	8
GTACATG	17390	0.0	19.963411	1
TACATGG	17280	0.0	19.9381	2
ACATGGG	17430	0.0	19.13658	3
GCAGAGT	23420	0.0	19.094261	9
AGAGTAC	20565	0.0	18.89624	10-11
GAGTACT	13375	0.0	17.865685	12-13
CATGGGG	12145	0.0	17.417973	4
CAGAGTA	22565	0.0	16.958862	10-11
AGTACTT	14925	0.0	15.980884	12-13
GTACTTT	15310	0.0	15.564915	14-15
GTGGTAT	4295	0.0	14.714895	1
>>END_MODULE