FastQCFastQC Report
Fri 27 May 2016
SRR2139434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6968155
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73450.048.176311
GTATCAA123700.034.5890661
TCAACGC148900.028.6759434
ATCAACG149000.028.5979143
CAACGCA152500.027.9990025
AACGCAG157800.027.0857736
TATCAAC161750.026.7501752
ACGCAGA181500.023.5248247
CGCAGAG186100.022.919818
GCAGAGT230600.018.686779
AGAGTAC200950.018.67631510-11
GAGTACT134800.018.33886512-13
GTACATG162800.018.0771051
TACATGG165400.017.6605552
CAGAGTA225250.017.3419710-11
ACATGGG169800.016.4805223
TATACCG4950.015.9248395
AGTACTT152600.015.78361812-13
GTACTTT158100.015.60864214-15
TATACTG41850.015.5918835
Produced by FastQC (version 0.11.5)