##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6968155
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.539475370453154	31.0	18.0	33.0	18.0	33.0
2	30.18565660494062	32.0	29.0	33.0	25.0	33.0
3	31.800287881081864	33.0	31.0	33.0	29.0	33.0
4	31.606078223001642	33.0	31.0	33.0	29.0	34.0
5	32.67329458084672	33.0	33.0	33.0	32.0	34.0
6	36.412335115966854	38.0	37.0	38.0	34.0	38.0
7	37.029092492919574	38.0	38.0	38.0	36.0	38.0
8	37.199830084147095	38.0	38.0	38.0	36.0	38.0
9	37.24158963168873	38.0	38.0	38.0	36.0	38.0
10-11	37.294419326206146	38.0	38.0	38.0	36.5	38.0
12-13	37.31039701326965	38.0	38.0	38.0	37.0	38.0
14-15	37.33127413210527	38.0	38.0	38.0	37.0	38.0
16-17	37.341457602478705	38.0	38.0	38.0	37.0	38.0
18-19	37.34376136868367	38.0	38.0	38.0	37.0	38.0
20-21	37.30658754187071	38.0	38.0	38.0	37.0	38.0
22-23	37.31802763759349	38.0	38.0	38.0	37.0	38.0
24-25	37.33853192846546	38.0	38.0	38.0	37.0	38.0
26-27	37.31338812346017	38.0	38.0	38.0	37.0	38.0
28-29	37.28981834276125	38.0	38.0	38.0	37.0	38.0
30-31	37.28760456743268	38.0	38.0	38.0	37.0	38.0
32-33	37.22616074035163	38.0	38.0	38.0	37.0	38.0
34-35	37.23628573630506	38.0	38.0	38.0	37.0	38.0
36-37	37.23490846718815	38.0	38.0	38.0	37.0	38.0
38-39	37.22875663588536	38.0	38.0	38.0	37.0	38.0
40-41	37.220408157368176	38.0	38.0	38.0	37.0	38.0
42-43	37.19632795291601	38.0	38.0	38.0	37.0	38.0
44-45	37.17648975883081	38.0	38.0	38.0	36.0	38.0
46-47	37.15433535592362	38.0	38.0	38.0	36.0	38.0
48-49	37.11697639986882	38.0	38.0	38.0	36.0	38.0
50-51	37.090216485877036	38.0	38.0	38.0	36.0	38.0
52-53	37.059047142577654	38.0	38.0	38.0	36.0	38.0
54-55	37.03651346635692	38.0	38.0	38.0	36.0	38.0
56-57	37.00613216720167	38.0	38.0	38.0	36.0	38.0
58-59	37.01146666850158	38.0	38.0	38.0	36.0	38.0
60-61	37.035576808556115	38.0	38.0	38.0	36.0	38.0
62-63	37.04173060568016	38.0	38.0	38.0	36.0	38.0
64-65	37.04078326007635	38.0	38.0	38.0	36.0	38.0
66-67	37.04477557868788	38.0	38.0	38.0	36.0	38.0
68-69	37.045816340353454	38.0	38.0	38.0	36.0	38.0
70-71	37.03324765882224	38.0	38.0	38.0	36.0	38.0
72-73	37.013332745030404	38.0	38.0	38.0	36.0	38.0
74-75	37.008069617607724	38.0	38.0	38.0	36.0	38.0
76-77	37.005702593700946	38.0	38.0	38.0	36.0	38.0
78-79	36.9791056952148	38.0	38.0	38.0	36.0	38.0
80-81	36.95492426854611	38.0	38.0	38.0	36.0	38.0
82-83	36.95479302370422	38.0	38.0	38.0	36.0	38.0
84-85	36.90519177919545	38.0	38.0	38.0	35.5	38.0
86-87	36.89122256544703	38.0	38.0	38.0	35.0	38.0
88-89	36.893957538492586	38.0	38.0	38.0	35.0	38.0
90-91	36.874858208101664	38.0	38.0	38.0	35.0	38.0
92-93	36.87876715554425	38.0	38.0	38.0	35.0	38.0
94-95	36.82579976346996	38.0	38.0	38.0	35.0	38.0
96-97	36.79931067553019	38.0	38.0	38.0	35.0	38.0
98-99	36.873271436399364	38.0	38.0	38.0	35.0	38.0
100	35.28330701574799	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	10.0
19	38.0
20	96.0
21	219.0
22	598.0
23	1608.0
24	3557.0
25	7130.0
26	12565.0
27	20354.0
28	30763.0
29	44122.0
30	60557.0
31	80278.0
32	104825.0
33	141112.0
34	208276.0
35	363377.0
36	1031020.0
37	4857647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.61352108269693	22.303550939954693	14.64997549566564	24.43295248168274
2	13.636362984025558	22.34345200585022	41.35494506771941	22.66523994240481
3	17.13825252165028	27.106543984742014	31.033738486012435	24.721465007595267
4	12.37905873218951	18.962767045222158	39.59049418389803	29.0676800386903
5	13.179847561903221	38.14801547458514	35.745582687937045	12.9265542755746
6	28.979873527152744	38.880900143165974	19.349823252588642	12.78940307709264
7	25.379831533598207	33.07106400474731	24.5046070301249	17.044497431529578
8	23.687317518051763	37.1180750141178	21.99034034116635	17.20426712666409
9	25.207404255502354	16.81575682515673	22.070820755278834	35.90601816406208
10-11	23.474714612404572	27.17468253791714	29.768475299415698	19.582127550262587
12-13	24.350541857923655	24.799283311005567	29.791429725659086	21.058745105411692
14-15	21.751432050521263	26.168160725471807	27.22117978144861	24.859227442558325
16-17	21.33397577981546	29.022065381725863	28.026134608084924	21.61782423037375
18-19	21.34935287748335	28.399203232419485	29.491572733384945	20.759871156712215
20-21	22.17579307484001	27.571781025330562	29.206347885132566	21.046078014696864
22-23	21.939873551245558	27.476986550575916	29.01487137698371	21.56826852119482
24-25	21.733054766343017	27.584231734362252	29.078914811546763	21.60379868774797
26-27	21.683317492036053	27.747587132637157	28.9888635345202	21.580231840806587
28-29	21.619695997631858	27.76867297816863	29.086733887459605	21.52489713673991
30-31	21.885249904485036	27.58745723371434	28.983699258574084	21.543593603226537
32-33	21.76567527089326	27.684097450611333	28.921602849208057	21.628624429287353
34-35	21.827068727692097	27.736989899986426	28.90082284271581	21.53511852960567
36-37	21.820888885359675	27.690864478442002	28.912668569073162	21.57557806712516
38-39	21.679797154470013	27.80429220724075	28.984304282207567	21.53160635608167
40-41	21.89306434617386	27.669507469158383	28.86310002981216	21.574328154855596
42-43	21.807649334232952	27.708073397499238	28.736476515442966	21.747800752824848
44-45	21.896168415348672	27.739652009121972	28.637090336546535	21.72708923898282
46-47	21.892754759065504	27.799993538935635	28.45435742238425	21.85289427961461
48-49	21.86883588841911	27.865191391154713	28.51816877700158	21.747803943424596
50-51	21.858396716224362	27.929064040883322	28.394071035481073	21.818468207411243
52-53	22.040684707361606	27.83652053481063	28.292917373520872	21.82987738430689
54-55	21.993025206174043	27.777156131656326	28.32847427992763	21.901344382242005
56-57	21.96588634027159	27.86774250857888	28.318475350017287	21.847895801132243
58-59	21.981818041150127	27.894528123405493	28.320347733659023	21.80330610178536
60-61	22.015688239602206	27.844351077361907	28.340908915699803	21.799051767336085
62-63	22.032257949406723	27.923385392028926	28.261537036568722	21.78281962199563
64-65	21.991131616645394	27.842601808806712	28.236105093465426	21.930161481082475
66-67	22.026688393482143	27.880337038115876	28.190535221948192	21.902439346453786
68-69	21.95918459770326	27.852104457584492	28.249446495399837	21.939264449312407
70-71	21.98571920715509	27.71639004899027	28.242122763386618	22.055767980468023
72-73	22.043859719599887	27.74919053549191	28.181617322138752	22.025332422769452
74-75	21.935717490667056	27.859975295565135	28.140390456303777	22.06391675746403
76-77	22.075326421149214	27.77285253749059	28.09903523713911	22.052785804221088
78-79	22.03549506697085	27.80179606662428	28.057481428117647	22.10522743828722
80-81	22.03005414490419	27.846595594940027	28.048619176975258	22.074731083180524
82-83	22.053380190462004	27.84886071286578	28.091726402770817	22.006032693901396
84-85	22.13949118575518	27.79945689369242	28.001527307692758	22.05952461285964
86-87	22.04739341637862	27.840103958701484	28.064676669237027	22.04782595568287
88-89	22.110898053228738	27.827419694188766	28.014399367782566	22.047282884799927
90-91	22.0965069491395	27.84578918832546	28.022239800272853	22.035464062262193
92-93	22.145859889744703	27.82125608028108	28.007295684405243	22.025588345568977
94-95	22.071757151385558	27.8711280980665	28.003086200555927	22.05402854999202
96-97	22.144195516152724	27.981940658853578	28.070420518697915	21.80344330629579
98-99	23.2184419527418	29.88653745330861	28.305913978752983	18.58910661519661
100	22.061933825630963	30.836149213614334	22.26250828547857	24.839408675276132
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	800.0
1	932.0
2	1541.5
3	2238.0
4	2924.0
5	3944.0
6	4757.0
7	5845.5
8	7007.0
9	7596.5
10	7698.0
11	7832.0
12	8769.0
13	10625.5
14	14098.5
15	18126.5
16	20750.5
17	21612.0
18	21010.5
19	19693.0
20	18532.0
21	18398.5
22	20421.0
23	24817.0
24	31797.0
25	42166.5
26	55686.5
27	71121.5
28	87636.5
29	105276.0
30	122121.5
31	139148.5
32	157325.5
33	174646.0
34	191609.5
35	207164.5
36	222457.0
37	237542.0
38	248857.5
39	257168.0
40	266554.5
41	275803.0
42	282466.0
43	287537.0
44	290359.0
45	292350.5
46	291871.5
47	287646.5
48	282447.0
49	275090.5
50	266079.5
51	253858.5
52	238466.0
53	222481.5
54	206131.5
55	189552.0
56	172315.5
57	153725.0
58	134594.5
59	115548.5
60	94971.0
61	75733.5
62	59555.0
63	45604.0
64	33932.0
65	24531.0
66	17801.0
67	13479.5
68	10213.0
69	7484.5
70	5305.0
71	3687.5
72	2613.0
73	1780.5
74	1138.0
75	684.0
76	412.0
77	247.5
78	146.0
79	83.0
80	49.0
81	30.5
82	20.0
83	13.5
84	7.5
85	3.0
86	3.0
87	3.5
88	3.0
89	3.0
90	3.0
91	2.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003042412231071209
3	0.0
4	0.0
5	0.004922393373855777
6	2.7266902070921214E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.577565983401294E-5
44-45	7.3464768170537E-6
46-47	0.0
48-49	0.0
50-51	1.4965705711220098E-5
52-53	0.0010081693315190298
54-55	0.002141039582904306
56-57	0.002496608646395118
58-59	0.001577964265237409
60-61	0.0038082040432642814
62-63	0.0015824790402978288
64-65	0.0016081492887140083
66-67	1.8863686130899207E-4
68-69	1.266780486893414E-4
70-71	1.994096835256653E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	9.038003902610084E-5
80-81	3.064593345028484E-4
82-83	8.349055300218253E-6
84-85	0.0
86-87	0.0
88-89	0.0
90-91	5.176779246084931E-5
92-93	2.437460211726499E-4
94-95	0.0
96-97	0.0
98-99	9.610989435984853E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	171.0
22-23	294.0
24-25	444.0
26-27	953.0
28-29	2285.0
30-31	7337.0
32-33	10094.0
34-35	14706.0
36-37	24293.0
38-39	29104.0
40-41	29024.0
42-43	33567.0
44-45	41307.0
46-47	42918.0
48-49	40291.0
50-51	36886.0
52-53	36012.0
54-55	39140.0
56-57	41555.0
58-59	41366.0
60-61	40797.0
62-63	40319.0
64-65	42530.0
66-67	45888.0
68-69	46738.0
70-71	45661.0
72-73	44363.0
74-75	44928.0
76-77	47530.0
78-79	48653.0
80-81	48632.0
82-83	46264.0
84-85	46614.0
86-87	48775.0
88-89	50640.0
90-91	51453.0
92-93	52555.0
94-95	65191.0
96-97	216188.0
98-99	489320.0
100-101	4933330.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.38042589485043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.99593453739404	77.73887154145176
2	7.202497485962662	12.443096006880886
3	1.6841102751781813	4.364224884713552
4	0.5552668557474684	1.918567499390432
5	0.2311828435731078	0.9984836243713818
6	0.12817398722902565	0.6643034163290614
7	0.06566607724173626	0.39705846032897263
8	0.038706795722483436	0.2674807599626476
9	0.02586547077904719	0.20108433436784143
>10	0.07140240682890875	0.9054153177298968
>50	8.57539305279397E-4	0.04789913122662956
>100	3.113290560094614E-4	0.042809759792892484
>500	2.439598211950091E-5	0.010705263454076675
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4351001089958532E-5	0.0	0.0	0.0
3	0.0	1.4351001089958532E-5	0.0	0.0	0.0
4	0.0	8.61060065397512E-5	0.0	1.4351001089958532E-5	0.0
5	0.0	8.61060065397512E-5	0.0	1.4351001089958532E-5	0.0
6	4.30530032698756E-5	8.61060065397512E-5	0.0	1.4351001089958532E-5	1.4351001089958532E-5
7	4.30530032698756E-5	8.61060065397512E-5	0.0	1.4351001089958532E-5	1.4351001089958532E-5
8	5.740400435983413E-5	8.61060065397512E-5	0.0	1.4351001089958532E-5	1.4351001089958532E-5
9	5.740400435983413E-5	1.4351001089958534E-4	0.0	2.8702002179917065E-5	4.30530032698756E-5
10-11	7.893050599477194E-5	1.4351001089958534E-4	0.0	4.30530032698756E-5	4.30530032698756E-5
12-13	8.61060065397512E-5	1.4351001089958534E-4	0.0	5.022850381485486E-5	4.30530032698756E-5
14-15	1.0045700762970973E-4	2.080895158043987E-4	0.0	5.740400435983413E-5	6.45795049048134E-5
16-17	1.8656301416946093E-4	2.367915179843158E-4	0.0	5.740400435983413E-5	7.175500544979267E-5
18-19	1.8656301416946093E-4	2.5114251907427433E-4	0.0	1.0763250817468899E-4	7.175500544979267E-5
20-21	1.8656301416946093E-4	2.583180196192536E-4	0.0	1.291590098096268E-4	8.610600653975121E-5
22-23	2.0091401525941947E-4	2.870200217991707E-4	0.0	1.291590098096268E-4	1.0045700762970973E-4
24-25	2.1526501634937798E-4	3.372485256140255E-4	0.0	1.291590098096268E-4	1.0045700762970973E-4
26-27	2.2961601743933652E-4	3.444240261590048E-4	0.0	1.5786101198954388E-4	1.0045700762970973E-4
28-29	2.2961601743933652E-4	3.444240261590048E-4	0.0	2.9419552234414995E-4	1.0045700762970973E-4
30-31	2.2961601743933652E-4	3.874770294288804E-4	0.0	7.606030577678023E-4	1.0045700762970973E-4
32-33	2.583180196192536E-4	3.874770294288804E-4	0.0	0.0019230341460544434	1.0045700762970973E-4
34-35	2.870200217991707E-4	4.448810337887145E-4	0.0	0.0033007302506904623	1.0045700762970973E-4
36-37	3.0137102288912916E-4	5.166360392385072E-4	0.0	0.0056614699299886405	1.0045700762970973E-4
38-39	3.0137102288912916E-4	5.166360392385072E-4	0.0	0.009220518200298358	1.0045700762970973E-4
40-41	3.3007302506904624E-4	5.596890425083828E-4	0.0	0.013116814996222099	1.0045700762970973E-4
42-43	3.3007302506904624E-4	5.596890425083828E-4	0.0	0.017156621803045426	1.0045700762970973E-4
44-45	3.5159952670398405E-4	6.314440479581755E-4	0.0	0.02164130964365747	1.0763250817468899E-4
46-47	3.7312602833892186E-4	7.247255550429059E-4	0.0	0.027324306075281045	1.2198350926464753E-4
48-49	3.7312602833892186E-4	7.319010555878852E-4	0.0	0.03302882900853957	1.291590098096268E-4
50-51	3.874770294288804E-4	7.893050599477193E-4	0.0	0.03879075594615791	1.291590098096268E-4
52-53	4.0182803051883894E-4	7.893050599477193E-4	0.0	0.043942765337453026	1.506855114445646E-4
54-55	4.0182803051883894E-4	7.964805604926986E-4	0.0	0.04933156624673246	1.7938751362448166E-4
56-57	4.0182803051883894E-4	8.180070621276364E-4	0.0	0.05416785361404848	1.937385147144402E-4
58-59	4.0182803051883894E-4	8.180070621276364E-4	0.0	0.058810402466650064	2.080895158043987E-4
60-61	4.233545321537767E-4	8.180070621276364E-4	0.0	0.06371844483941588	2.1526501634937798E-4
62-63	4.5923203487867304E-4	8.180070621276364E-4	0.0	0.06864801371381665	2.1526501634937798E-4
64-65	4.5923203487867304E-4	9.112885692123668E-4	0.0	0.07324750956314835	2.1526501634937798E-4
66-67	4.664075354236523E-4	9.471660719372632E-4	0.0	0.07758151189231582	2.1526501634937798E-4
68-69	4.735830359686316E-4	9.615170730272217E-4	0.0	0.08231016675145716	2.1526501634937798E-4
70-71	5.238115397834865E-4	9.68692573572201E-4	0.0	0.08611318204029617	2.1526501634937798E-4
72-73	5.883910446882999E-4	9.758680741171802E-4	0.0	0.0892201737762722	2.4396701852929506E-4
74-75	5.883910446882999E-4	9.830435746621595E-4	0.0	0.09190381098009445	2.4396701852929506E-4
76-77	6.099175463232376E-4	0.001047623079566973	0.0	0.09456592168228176	2.4396701852929506E-4
78-79	6.314440479581754E-4	0.001105027083926807	0.0	0.09630239281416673	2.4396701852929506E-4
80-81	6.314440479581754E-4	0.001105027083926807	0.0	0.09679032685122532	2.583180196192536E-4
82-83	6.386195485031547E-4	0.001105027083926807	0.0	0.09710604887520441	2.870200217991707E-4
84-85	6.457950490481339E-4	0.0011193780850167656	0.0	0.0973141383910088	2.870200217991707E-4
86-87	6.457950490481339E-4	0.0011193780850167656	0.0	0.09740741989809354	2.870200217991707E-4
88	6.601460501380925E-4	0.0011624310882866412	0.0	0.09741459539863852	2.870200217991707E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7345	0.0	48.17631	1
GTATCAA	12370	0.0	34.589066	1
TCAACGC	14890	0.0	28.675943	4
ATCAACG	14900	0.0	28.597914	3
CAACGCA	15250	0.0	27.999002	5
AACGCAG	15780	0.0	27.085773	6
TATCAAC	16175	0.0	26.750175	2
ACGCAGA	18150	0.0	23.524824	7
CGCAGAG	18610	0.0	22.91981	8
GCAGAGT	23060	0.0	18.68677	9
AGAGTAC	20095	0.0	18.676315	10-11
GAGTACT	13480	0.0	18.338865	12-13
GTACATG	16280	0.0	18.077105	1
TACATGG	16540	0.0	17.660555	2
CAGAGTA	22525	0.0	17.34197	10-11
ACATGGG	16980	0.0	16.480522	3
TATACCG	495	0.0	15.924839	5
AGTACTT	15260	0.0	15.783618	12-13
GTACTTT	15810	0.0	15.608642	14-15
TATACTG	4185	0.0	15.591883	5
>>END_MODULE