FastQCFastQC Report
Fri 27 May 2016
SRR2139433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6479729
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64950.047.8587881
GTATCAA114400.034.6341931
TCAACGC138250.028.1836554
ATCAACG138450.028.07973
CAACGCA142400.027.270065
TATCAAC150300.026.2451042
AACGCAG149250.026.1339866
CGCAGAG170200.022.9428628
ACGCAGA170050.022.8858687
GCAGAGT209800.018.5915039
AGAGTAC189500.018.42314510-11
GTACATG153400.018.3513551
TACATGG150850.018.0230712
ACATGGG150700.017.4014743
GAGTACT130400.017.12122512-13
GTGGTAT37350.016.87931
CAGAGTA207150.016.87455410-11
TGGTATC36150.015.744092
AGTACTT145850.015.53267312-13
GTACTTT147750.015.08120214-15
Produced by FastQC (version 0.11.5)