##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139433_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6479729
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.866038070419304	30.0	18.0	33.0	18.0	33.0
2	29.894490957878023	32.0	28.0	33.0	25.0	33.0
3	31.670383745986907	33.0	31.0	33.0	29.0	33.0
4	31.525519971591404	33.0	31.0	33.0	29.0	33.0
5	32.64215988045179	33.0	33.0	33.0	31.0	34.0
6	36.39233585231728	38.0	37.0	38.0	34.0	38.0
7	37.01414704226056	38.0	38.0	38.0	36.0	38.0
8	37.18102269400464	38.0	38.0	38.0	36.0	38.0
9	37.21854463357958	38.0	38.0	38.0	36.0	38.0
10-11	37.27675185181356	38.0	38.0	38.0	36.5	38.0
12-13	37.295499163622424	38.0	38.0	38.0	37.0	38.0
14-15	37.314462765340956	38.0	38.0	38.0	37.0	38.0
16-17	37.325015984464784	38.0	38.0	38.0	37.0	38.0
18-19	37.32537487293064	38.0	38.0	38.0	37.0	38.0
20-21	37.2880489580628	38.0	38.0	38.0	37.0	38.0
22-23	37.300046292622824	38.0	38.0	38.0	37.0	38.0
24-25	37.320778474106774	38.0	38.0	38.0	37.0	38.0
26-27	37.29447634641385	38.0	38.0	38.0	37.0	38.0
28-29	37.27200410014589	38.0	38.0	38.0	37.0	38.0
30-31	37.268508809505576	38.0	38.0	38.0	37.0	38.0
32-33	37.20551970620506	38.0	38.0	38.0	36.5	38.0
34-35	37.21753571947788	38.0	38.0	38.0	37.0	38.0
36-37	37.21308804806448	38.0	38.0	38.0	36.5	38.0
38-39	37.20272076866442	38.0	38.0	38.0	36.5	38.0
40-41	37.198529985536794	38.0	38.0	38.0	36.5	38.0
42-43	37.1747258180028	38.0	38.0	38.0	36.0	38.0
44-45	37.152297404279714	38.0	38.0	38.0	36.0	38.0
46-47	37.13013652990149	38.0	38.0	38.0	36.0	38.0
48-49	37.09194055824587	38.0	38.0	38.0	36.0	38.0
50-51	37.06488535687943	38.0	38.0	38.0	36.0	38.0
52-53	37.03132629775164	38.0	38.0	38.0	36.0	38.0
54-55	37.00414793188861	38.0	38.0	38.0	36.0	38.0
56-57	36.97245340629634	38.0	38.0	38.0	36.0	38.0
58-59	36.97604909378691	38.0	38.0	38.0	36.0	38.0
60-61	36.999330066836364	38.0	38.0	38.0	36.0	38.0
62-63	37.00636842082916	38.0	38.0	38.0	36.0	38.0
64-65	37.00226090487899	38.0	38.0	38.0	36.0	38.0
66-67	37.0044386614107	38.0	38.0	38.0	36.0	38.0
68-69	37.00237104733239	38.0	38.0	38.0	36.0	38.0
70-71	36.989850565396225	38.0	38.0	38.0	36.0	38.0
72-73	36.9675281228432	38.0	38.0	38.0	36.0	38.0
74-75	36.96187720751385	38.0	38.0	38.0	36.0	38.0
76-77	36.96082952675373	38.0	38.0	38.0	36.0	38.0
78-79	36.93438660888508	38.0	38.0	38.0	36.0	38.0
80-81	36.90721359658802	38.0	38.0	38.0	35.5	38.0
82-83	36.90548046923046	38.0	38.0	38.0	35.0	38.0
84-85	36.85384155593387	38.0	38.0	38.0	35.0	38.0
86-87	36.83967380979901	38.0	38.0	38.0	35.0	38.0
88-89	36.84184655135196	38.0	38.0	38.0	35.0	38.0
90-91	36.81874350100627	38.0	38.0	38.0	35.0	38.0
92-93	36.822692581625915	38.0	38.0	38.0	35.0	38.0
94-95	36.769965684845104	38.0	38.0	38.0	35.0	38.0
96-97	36.7465554055363	38.0	38.0	38.0	35.0	38.0
98-99	36.822187190062834	38.0	38.0	38.0	35.0	38.0
100	35.18393345323467	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	16.0
19	54.0
20	95.0
21	228.0
22	602.0
23	1603.0
24	3642.0
25	7116.0
26	12326.0
27	19647.0
28	29963.0
29	43096.0
30	58700.0
31	77207.0
32	101810.0
33	136802.0
34	201940.0
35	351151.0
36	998894.0
37	4434833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.868636172901674	22.860631980133736	14.756774550293692	24.513957296670892
2	13.475729288106653	22.51660535056666	41.54561636956215	22.462048991764537
3	17.166381495275495	27.09279971430904	31.08725688991006	24.65356190050541
4	12.260451015775505	19.24106702610557	39.775444312563074	28.723037645555856
5	13.162603163089317	38.13170150129062	35.80151016486802	12.904185170752033
6	28.69764662525333	39.12649875395773	19.493431502244853	12.682423118544083
7	25.24449710782658	33.01767712816385	24.583250318030274	17.1545754459793
8	23.400453938737254	37.31751744555984	22.130724911489356	17.151303704213554
9	25.043454749419304	17.04801234742996	22.113918653079473	35.79461425007126
10-11	23.321476870406155	27.297985764528114	29.891250081600635	19.489287283465096
12-13	24.24211722434688	24.901481219353464	29.80636998862144	21.050031567678214
14-15	21.54439637830533	26.26064299911308	27.36833592886369	24.8266246937179
16-17	21.215432003406313	29.077589510302047	28.18839337262407	21.518585113667562
18-19	21.215185079499467	28.488737723444917	29.594401247336116	20.701675949719505
20-21	22.018564897352448	27.66646428941819	29.345992251430943	20.96897856179842
22-23	21.720594581146678	27.636480677686944	29.136468639631868	21.506456101534514
24-25	21.545591053617823	27.738950566379796	29.214927367596864	21.500531012405517
26-27	21.53292704630317	27.898129107034453	29.148385525364695	21.420558321297687
28-29	21.442118265195962	27.933770094667302	29.228191561378285	21.39592007875845
30-31	21.720967876460758	27.772952776945438	29.14992632499174	21.35615302160207
32-33	21.658756798318265	27.806071701793645	29.058419631884764	21.476751868003323
34-35	21.669009176168924	27.870827281538563	29.06071008740525	21.39945345488726
36-37	21.651145356127486	27.849123143113125	29.050104178326343	21.449627322433045
38-39	21.48748121994051	27.930852138454977	29.148272927956487	21.433393713648027
40-41	21.7439954909753	27.779072185548493	29.038574412129805	21.438357911346404
42-43	21.691932704570043	27.836109808062975	28.8793916978416	21.592565789525377
44-45	21.720935074421273	27.924105845273512	28.76992276060083	21.585036319704386
46-47	21.743112842402784	27.9137800215267	28.585052922258058	21.758054213812457
48-49	21.724582862810717	27.990720635670524	28.59880031326771	21.685896188251053
50-51	21.704040894806734	28.044910680743406	28.497175046730018	21.753873377719845
52-53	21.863551641746582	27.995227932686127	28.45190277410174	21.689317651465547
54-55	21.859635553207905	27.93238537529095	28.441140060654398	21.766839010846756
56-57	21.84919653327395	28.033045781184068	28.352253046327675	21.76550463921431
58-59	21.78023747981731	28.064574395951837	28.486998939945735	21.668189184285115
60-61	21.890409388178682	28.101620542166888	28.407631449397762	21.60033862025667
62-63	21.825240387521063	28.196346451467956	28.36379763307542	21.614615527935555
64-65	21.79531214291635	28.078029604657118	28.3608021023743	21.765856150052233
66-67	21.837856184451095	28.102801562546986	28.27434079837758	21.785001454624336
68-69	21.82905801222438	28.082301764456552	28.353477110926946	21.73516311239213
70-71	21.82863106192965	27.92126721180172	28.40132260439139	21.848779121877243
72-73	21.891256590319884	27.905343007630645	28.328522964937726	21.87487743711174
74-75	21.851788178641947	27.95472614368315	28.28057798441152	21.912907693263385
76-77	21.903822009145827	27.9282883017115	28.249790198144982	21.91809949099769
78-79	21.864001808998708	27.928590482617537	28.229522010956455	21.9778856974273
80-81	21.853020045375846	27.990075113290487	28.199192536801437	21.957712304532233
82-83	21.93807500083227	27.977293706344536	28.209324239114743	21.875307053708454
84-85	22.053822655299342	27.94211276315679	28.13514195607557	21.868922625468297
86-87	21.945026248151986	27.94438902347688	28.202851434603087	21.907733293768047
88-89	21.94638943428447	27.952048601982682	28.160612446438183	21.940949517294666
90-91	21.998985848123205	27.977551115173156	28.142325306963528	21.88113772974011
92-93	22.01362226415913	27.95120384104167	28.16708104918062	21.868092845618577
94-95	21.97456257479361	28.02523920267725	28.117997907147657	21.882200315381482
96-97	22.010023626284774	28.158764957194172	28.1801824710909	21.65102894543015
98-99	23.091387634932563	30.06158863646663	28.45375804439176	18.393265684209048
100	21.92809795130694	31.00242727271342	22.442529980595317	24.62694479538433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	633.0
1	787.0
2	1411.5
3	2045.5
4	2666.5
5	3691.0
6	4481.0
7	5425.0
8	6461.5
9	6884.0
10	6922.0
11	7166.5
12	8101.0
13	9939.5
14	13538.0
15	17679.0
16	20215.0
17	20955.0
18	20315.5
19	19018.0
20	17753.0
21	17568.0
22	19561.0
23	23855.0
24	30648.5
25	40255.5
26	52778.0
27	67083.5
28	81742.0
29	97696.0
30	113983.5
31	130170.5
32	147222.0
33	163777.0
34	181047.0
35	197134.5
36	212533.0
37	226942.5
38	237755.0
39	247625.0
40	256467.5
41	263640.5
42	268306.0
43	272910.0
44	275687.0
45	275889.5
46	273426.5
47	269275.0
48	265475.5
49	257574.5
50	247090.5
51	234623.0
52	219741.5
53	203053.0
54	185576.0
55	168399.5
56	150917.5
57	133356.0
58	115911.0
59	98287.5
60	80591.5
61	64058.0
62	49828.0
63	37827.5
64	27731.0
65	20036.0
66	14570.5
67	10897.5
68	8144.0
69	5920.0
70	4344.5
71	3076.0
72	2112.5
73	1457.5
74	992.5
75	622.5
76	371.0
77	221.5
78	149.0
79	96.5
80	55.0
81	35.0
82	22.0
83	11.0
84	6.0
85	5.5
86	5.5
87	5.0
88	2.5
89	0.0
90	0.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002947654138004846
3	0.0
4	0.0
5	0.004691554230122895
6	2.0062567431446593E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.857570533481894E-5
44-45	1.579666781929686E-5
46-47	0.0
48-49	0.0
50-51	8.042685426927457E-6
52-53	0.001148284717357399
54-55	0.00201656838606226
56-57	0.002544234294691214
58-59	0.0017206238669300784
60-61	0.004042865642816466
62-63	0.0015255189306895896
64-65	0.0014681637910371704
66-67	1.6049836262104638E-4
68-69	1.3615337609740685E-4
70-71	9.430997889599881E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	7.066396212128975E-5
80-81	2.84900310265341E-4
82-83	0.0
84-85	9.042449780494532E-6
86-87	0.0
88-89	0.0
90-91	1.8536861429453387E-5
92-93	2.3374410275315958E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	50.0
20-21	170.0
22-23	300.0
24-25	375.0
26-27	899.0
28-29	2040.0
30-31	6703.0
32-33	9152.0
34-35	13578.0
36-37	22288.0
38-39	26976.0
40-41	26613.0
42-43	31019.0
44-45	38288.0
46-47	39149.0
48-49	36706.0
50-51	34029.0
52-53	33441.0
54-55	36504.0
56-57	38616.0
58-59	38032.0
60-61	37681.0
62-63	37509.0
64-65	39914.0
66-67	42965.0
68-69	43721.0
70-71	44826.0
72-73	40984.0
74-75	41718.0
76-77	43736.0
78-79	44651.0
80-81	44288.0
82-83	42660.0
84-85	42924.0
86-87	44378.0
88-89	46436.0
90-91	46905.0
92-93	48067.0
94-95	60509.0
96-97	201690.0
98-99	456018.0
100-101	4593221.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.26725747064508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.95218119855126	82.08319034038485
2	6.108168457196653	10.905188926852931
3	1.1919794278222697	3.1921420344936826
4	0.3813468077508923	1.3616713469242996
5	0.14947756941439902	0.6671726337500689
6	0.08025093788682246	0.42982686807622356
7	0.04135954261956929	0.2584437057922447
8	0.027421768388483016	0.1958292847228088
9	0.014263697342055149	0.11459530288049334
>10	0.05269350094770192	0.7031553448466777
>50	5.402355755570676E-4	0.03465839610898279
>100	3.1685650432499037E-4	0.05412581516675237
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0865488356071684E-5	0.0	0.0	0.0
3	0.0	3.0865488356071684E-5	0.0	0.0	0.0
4	0.0	1.0802920924625088E-4	0.0	1.5432744178035842E-5	0.0
5	0.0	1.0802920924625088E-4	0.0	1.5432744178035842E-5	0.0
6	0.0	1.0802920924625088E-4	0.0	3.0865488356071684E-5	1.5432744178035842E-5
7	0.0	1.0802920924625088E-4	0.0	3.0865488356071684E-5	1.5432744178035842E-5
8	0.0	1.0802920924625088E-4	0.0	3.0865488356071684E-5	1.5432744178035842E-5
9	0.0	1.2346195342428673E-4	0.0	4.6298232534107525E-5	1.5432744178035842E-5
10-11	0.0	1.2346195342428673E-4	0.0	5.4014604623125446E-5	1.5432744178035842E-5
12-13	0.0	1.3117832551330465E-4	0.0	6.173097671214337E-5	1.5432744178035842E-5
14-15	0.0	1.6204381386937633E-4	0.0	8.488009297919714E-5	1.5432744178035842E-5
16-17	1.5432744178035842E-5	1.6976018595839425E-4	0.0	1.157455813352688E-4	1.5432744178035842E-5
18-19	1.5432744178035842E-5	1.774765580474122E-4	0.0	1.543274417803584E-4	1.5432744178035842E-5
20-21	1.5432744178035842E-5	2.0062567431446593E-4	0.0	1.774765580474122E-4	1.5432744178035842E-5
22-23	1.5432744178035842E-5	2.3920753475955555E-4	0.0	1.9290930222544802E-4	2.3149116267053763E-5
24-25	1.5432744178035842E-5	2.546402789375914E-4	0.0	2.2377479058151967E-4	3.0865488356071684E-5
26-27	1.5432744178035842E-5	2.623566510266093E-4	0.0	2.546402789375914E-4	3.0865488356071684E-5
28-29	1.5432744178035842E-5	2.623566510266093E-4	0.0	3.703858602728602E-4	3.0865488356071684E-5
30-31	2.3149116267053763E-5	2.9322213938268095E-4	0.0	6.79040743833577E-4	3.0865488356071684E-5
32-33	3.0865488356071684E-5	2.9322213938268095E-4	0.0	0.001342648743489118	3.0865488356071684E-5
34-35	6.173097671214337E-5	3.240876277387526E-4	0.0	0.0026621483707111827	3.0865488356071684E-5
36-37	6.173097671214337E-5	3.7810223236187806E-4	0.0	0.004838165299814236	3.0865488356071684E-5
38-39	6.173097671214337E-5	4.0125134862893186E-4	0.0	0.008773515065213375	3.0865488356071684E-5
40-41	6.173097671214337E-5	4.321168369850035E-4	0.0	0.012191867900648315	3.0865488356071684E-5
42-43	6.173097671214337E-5	4.4754958116303934E-4	0.0	0.01584171189875379	3.0865488356071684E-5
44-45	6.173097671214337E-5	4.8613144160812897E-4	0.0	0.019977687338467394	3.0865488356071684E-5
46-47	6.173097671214337E-5	5.169969299642007E-4	0.0	0.024839001754548687	3.0865488356071684E-5
48-49	6.173097671214337E-5	5.247133020532186E-4	0.0	0.0297543307752531	3.0865488356071684E-5
50-51	9.259646506821505E-5	5.632951624983081E-4	0.0	0.03456934695880028	3.0865488356071684E-5
52-53	9.259646506821505E-5	5.710115345873261E-4	0.0	0.03963128704919604	3.8581860445089604E-5
54-55	9.259646506821505E-5	5.710115345873261E-4	0.0	0.04447716872109929	4.6298232534107525E-5
56-57	9.259646506821505E-5	5.710115345873261E-4	0.0	0.048566845928278785	4.6298232534107525E-5
58-59	9.259646506821505E-5	6.018770229433977E-4	0.0	0.05279541783306061	4.6298232534107525E-5
60-61	9.259646506821505E-5	6.095933950324157E-4	0.0	0.057108869830821626	4.6298232534107525E-5
62-63	9.259646506821505E-5	6.481752554775053E-4	0.0	0.06082044480563925	4.6298232534107525E-5
64-65	9.259646506821505E-5	6.79040743833577E-4	0.0	0.06450887066418981	4.6298232534107525E-5
66-67	1.0802920924625088E-4	6.86757115922595E-4	0.0	0.06869114433643753	4.6298232534107525E-5
68-69	1.0802920924625088E-4	7.021898601006307E-4	0.0	0.0728116870319731	4.6298232534107525E-5
70-71	1.0802920924625088E-4	7.176226042786666E-4	0.0	0.07649239651843465	4.6298232534107525E-5
72-73	1.2346195342428673E-4	7.407717205457204E-4	0.0	0.0793937524239054	6.173097671214337E-5
74-75	1.2346195342428673E-4	7.484880926347383E-4	0.0	0.08149260563211826	6.173097671214337E-5
76-77	1.3117832551330465E-4	7.562044647237562E-4	0.0	0.08352972786361898	6.173097671214337E-5
78-79	1.3889469760232256E-4	7.639208368127742E-4	0.0	0.08497268944426534	6.173097671214337E-5
80-81	1.3889469760232256E-4	7.793535809908099E-4	0.0	0.08549740274631856	6.944734880116128E-5
82-83	1.3889469760232256E-4	8.102190693468816E-4	0.0	0.08587550497868043	9.259646506821505E-5
84-85	1.3889469760232256E-4	8.179354414358995E-4	0.0	0.08612242888552901	9.259646506821505E-5
86-87	1.6204381386937633E-4	8.333681856139354E-4	0.0	0.08622274172268624	9.259646506821505E-5
88	1.6976018595839425E-4	9.259646506821505E-4	0.0	0.08625360721104232	9.259646506821505E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6495	0.0	47.858788	1
GTATCAA	11440	0.0	34.634193	1
TCAACGC	13825	0.0	28.183655	4
ATCAACG	13845	0.0	28.0797	3
CAACGCA	14240	0.0	27.27006	5
TATCAAC	15030	0.0	26.245104	2
AACGCAG	14925	0.0	26.133986	6
CGCAGAG	17020	0.0	22.942862	8
ACGCAGA	17005	0.0	22.885868	7
GCAGAGT	20980	0.0	18.591503	9
AGAGTAC	18950	0.0	18.423145	10-11
GTACATG	15340	0.0	18.351355	1
TACATGG	15085	0.0	18.023071	2
ACATGGG	15070	0.0	17.401474	3
GAGTACT	13040	0.0	17.121225	12-13
GTGGTAT	3735	0.0	16.8793	1
CAGAGTA	20715	0.0	16.874554	10-11
TGGTATC	3615	0.0	15.74409	2
AGTACTT	14585	0.0	15.532673	12-13
GTACTTT	14775	0.0	15.081202	14-15
>>END_MODULE