FastQCFastQC Report
Fri 27 May 2016
SRR2139432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12953995
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177100.057.2653731
GTATCAA324250.041.466471
ATCAACG393800.033.356833
TCAACGC395000.033.277094
TATCAAC408400.032.439082
CAACGCA409700.030.5793085
AACGCAG428050.029.4219676
ACGCAGA488100.025.7903657
TGGTATC75650.025.6566722
CGCAGAG492950.025.54098
GTGGTAT83500.024.7079811
GCAGAGT566250.022.0059289
GTACATG371100.020.8574811
GAGTACT354200.020.6667512-13
TACATGG365300.020.5968532
CAGAGTA552900.019.76256210-11
ACATGGG377950.019.2729433
AGAGTAC520650.019.05811710-11
CTTATAC96650.019.0122761
GTACTTT389650.018.84222614-15
Produced by FastQC (version 0.11.5)