FastQCFastQC Report
Fri 27 May 2016
SRR2139431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15301385
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211450.052.8732341
GTATCAA373700.038.596291
ATCAACG454650.031.158143
TCAACGC459250.030.915534
TATCAAC474100.030.075252
CAACGCA475100.028.4900655
AACGCAG494800.027.5069736
ACGCAGA555500.024.4897827
CGCAGAG562100.024.2192788
GTGGTAT111950.022.6644211
TGGTATC105300.022.4416752
GCAGAGT650250.020.6704489
GTACATG393550.020.5495911
TACATGG387950.020.4336642
GAGTACT416250.019.86545412-13
ACATGGG400950.019.2736973
AGAGTAC579350.019.24096510-11
CAGAGTA630850.018.97456410-11
GTACTTT451600.018.19855114-15
AGTACTT443950.017.71382712-13
Produced by FastQC (version 0.11.5)