##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139431_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15301385
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.850928004229683	33.0	31.0	34.0	30.0	34.0
2	31.515304594976207	33.0	31.0	34.0	30.0	34.0
3	31.831724971301618	33.0	31.0	34.0	30.0	34.0
4	32.95451411751289	37.0	35.0	37.0	26.0	37.0
5	34.430114398141086	37.0	35.0	37.0	28.0	37.0
6	35.17780017952623	37.0	35.0	37.0	32.0	37.0
7	35.32060862464411	37.0	35.0	37.0	32.0	37.0
8	35.45398740048695	37.0	35.0	37.0	32.0	37.0
9	37.22385398445957	39.0	37.0	39.0	33.0	39.0
10-11	37.09024568691004	39.0	37.0	39.0	33.0	39.0
12-13	36.98821417146226	39.0	37.0	39.0	32.5	39.0
14-15	38.34673325323165	40.0	38.0	41.0	33.0	41.0
16-17	38.344129861447186	40.0	38.0	41.0	33.0	41.0
18-19	38.27454001712917	40.0	38.0	41.0	33.0	41.0
20-21	38.338814695376215	40.0	38.0	41.0	33.0	41.0
22-23	38.38494524284057	40.0	38.0	41.0	33.0	41.0
24-25	38.26687471031097	40.0	38.0	41.0	32.5	41.0
26-27	38.172675310960564	40.0	38.0	41.0	32.0	41.0
28-29	38.184880028011975	40.0	38.0	41.0	32.0	41.0
30-31	38.13307616466247	40.0	37.5	41.0	32.0	41.0
32-33	38.03087147954797	40.0	37.0	41.0	32.0	41.0
34-35	37.92523151975107	40.0	37.0	41.0	32.0	41.0
36-37	37.8847377656292	40.0	37.0	41.0	31.5	41.0
38-39	37.82572525997002	40.0	37.0	41.0	31.0	41.0
40-41	37.732454484772546	40.0	37.0	41.0	31.0	41.0
42-43	37.70039356589231	40.0	37.0	41.0	31.0	41.0
44-45	37.633697392446464	40.0	37.0	41.0	31.0	41.0
46-47	37.499276014387085	40.0	36.0	41.0	30.0	41.0
48-49	37.66787966079792	40.0	37.0	41.0	31.0	41.0
50-51	37.63336229567988	40.0	36.5	41.0	31.0	41.0
52-53	37.56861614910733	40.0	36.0	41.0	31.0	41.0
54-55	37.444456226941426	39.5	36.0	41.0	30.5	41.0
56-57	37.313917088328935	39.0	36.0	41.0	30.0	41.0
58-59	37.206120058247265	39.0	35.0	41.0	30.5	41.0
60-61	36.99774988544986	39.0	35.0	41.0	31.0	41.0
62-63	36.80992059204561	39.0	35.0	41.0	30.5	41.0
64-65	36.54669706611415	38.0	35.0	40.0	30.0	41.0
66-67	36.32909804870766	37.5	35.0	40.0	30.0	41.0
68-69	36.073889724630604	37.0	34.5	40.0	30.0	41.0
70-71	35.820578646627375	36.5	34.0	39.0	30.0	41.0
72-73	35.51281273972527	36.0	34.0	39.0	30.0	41.0
74-75	35.33069139356435	36.0	34.0	39.0	30.0	40.5
76-77	35.13112304849949	35.5	34.0	38.5	30.0	40.0
78-79	34.872562094811045	35.0	34.0	37.0	30.0	39.0
80-81	34.616645161195294	35.0	34.0	37.0	30.0	39.0
82-83	34.36700389130205	35.0	34.0	36.0	30.0	39.0
84-85	34.15289781076606	35.0	34.0	36.0	30.0	37.0
86-87	33.96438423246252	35.0	34.0	36.0	30.0	37.0
88-89	33.82307716254002	35.0	34.0	35.0	29.5	37.0
90-91	33.687399956758796	35.0	34.0	35.0	29.0	36.0
92-93	33.64068601037745	35.0	34.0	35.0	29.0	36.0
94-95	33.613651401718755	35.0	34.0	35.0	29.5	36.0
96-97	33.615830328272295	35.0	34.0	35.0	30.0	36.0
98-99	33.64155796776359	35.0	34.0	35.0	30.0	35.5
100-101	33.2516087402215	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	13.0
17	87.0
18	578.0
19	2137.0
20	5933.0
21	13484.0
22	25151.0
23	42249.0
24	68545.0
25	109362.0
26	170481.0
27	249420.0
28	284743.0
29	228637.0
30	270040.0
31	335139.0
32	421924.0
33	558271.0
34	766529.0
35	1095241.0
36	1666396.0
37	2897817.0
38	3883746.0
39	2102403.0
40	103057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.83972137162747	20.170749249169276	14.787125479164139	24.202403900039112
2	14.3270307144521	20.57407074639779	41.895477687930516	23.203420851219594
3	18.001316961185594	25.57862797480454	30.344275875591286	26.075779188418586
4	12.098221161664318	18.024666329013815	38.65477813413606	31.222334375185813
5	13.221928587482271	37.521056185795175	35.448614476016225	13.80840075070633
6	30.007708382712504	38.04017541573991	18.588759024942565	13.363357176605026
7	26.4791427695647	32.69175191505303	22.816150528486716	18.01295478689554
8	24.130592015595013	37.35066876330728	20.70959577756221	17.809143443535493
9	25.093039267488752	17.225784547557453	21.252041080075692	36.4291351048781
10-11	24.096452355593186	26.987267964721223	28.698658886865186	20.217620792820405
12-13	24.97094480826327	24.488076480915026	28.902512059252565	21.638466651569143
14-15	21.98116358393102	25.918712580764407	26.72818577079839	25.37193806450618
16-17	21.471051785076853	29.053078164696235	27.397424463893987	22.078445586332926
18-19	21.44424532534977	28.01638281796764	29.38179826128442	21.157573595398173
20-21	22.21942655447669	27.155945415217282	29.41571390190069	21.208914128405336
22-23	21.917176578512652	27.094509923700855	29.281478007346706	21.70683549043979
24-25	21.70899673833755	27.175832965723572	29.37916910334042	21.73600119259846
26-27	21.70642861648074	27.346527338064085	29.27157527766469	21.675468767790488
28-29	21.579889373631342	27.401609225078627	29.36146121177351	21.657040189516522
30-31	21.875906439754335	27.236357676195507	29.240352204232106	21.647383679818052
32-33	21.651628749857544	27.362276577344502	29.237038306852302	21.749056365945652
34-35	21.7899040829482	27.40288552400896	29.174171347895722	21.63303904514712
36-37	21.8125840337835	27.30935120591525	29.135841545427017	21.74222321487423
38-39	21.647695790754877	27.46061896050921	29.179128933350423	21.71255631538549
40-41	21.913874974628573	27.344835046061146	29.04785029898606	21.693439680324218
42-43	21.800488540348287	27.422118394463634	28.90974745748722	21.867645607700855
44-45	21.89876070775577	27.561963595764915	28.678052358379524	21.86122333809979
46-47	22.01374592420644	27.57850802062145	28.329560234035313	22.0781858211368
48-49	21.91451753599891	27.680449956004537	28.35887186013773	22.046160647858816
50-51	21.898512690174535	27.816222031028957	28.227558458947676	22.05770681984883
52-53	22.05593353959649	27.778047207384322	28.111963933685647	22.05405531933354
54-55	22.02097087724124	27.68634425343824	28.159770184411098	22.132914684909423
56-57	22.014288409670982	27.80836556164464	28.07449583189761	22.10285019678677
58-59	21.987911773671716	27.86176696512929	28.12372411955882	22.02659714164017
60-61	22.10400248211005	27.80469437861382	28.097111360791345	21.994191778484783
62-63	21.98485334160662	27.887888640159574	28.09446518730544	22.03279283092836
64-65	21.94658025046663	27.871456467186096	28.07299907974202	22.108964202605257
66-67	22.052914203540304	27.903081122498453	27.922023888697556	22.121980785263695
68-69	22.00380944119654	27.857612727422055	27.990883514293735	22.147694317087673
70-71	22.016933310412966	27.630090155921504	28.06634498587893	22.286631547786595
72-73	22.02539047094594	27.708023507859224	28.052310869932935	22.2142751512619
74-75	21.908304181699283	27.820182221196244	28.015995503532555	22.25551809357192
76-77	22.04339598590657	27.810664861283662	27.911599987171208	22.234339165638552
78-79	22.004311234794127	27.837161782630517	27.89149015914738	22.267036823427976
80-81	21.997589483340413	27.81248457327813	27.92304979086487	22.266876152516595
82-83	22.01980363268124	27.822842513654756	28.030009343550837	22.127344510113176
84-85	22.13654527211969	27.78370067573286	27.949673568776173	22.13008048337128
86-87	21.958709940627376	27.821520759084727	28.044605040510874	22.17516425977702
88-89	22.02473714548601	27.82242293338268	27.957278393933965	22.195561527197345
90-91	22.06561364341049	27.846475470049825	27.96758026223458	22.120330624305105
92-93	22.06176428918991	27.8066754049457	27.984795003888323	22.146765301976064
94-95	21.90852204781821	27.89168056712584	28.035773671826114	22.164023713229835
96-97	21.929298673140654	27.933176210359033	28.047244507182008	22.09028060931831
98-99	22.21699273951363	28.479338602313888	28.055358329278995	21.248310328893492
100-101	22.860985268596764	31.053956354200047	25.293469364338232	20.791589012864957
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6681.0
1	5139.5
2	4809.0
3	6130.5
4	7305.5
5	10912.0
6	14706.5
7	19189.5
8	23362.0
9	23964.0
10	20348.5
11	16201.0
12	16794.0
13	22474.5
14	37209.0
15	57464.5
16	73762.0
17	83440.0
18	86196.0
19	82798.5
20	76386.0
21	69997.0
22	67596.0
23	73497.0
24	87086.5
25	108221.0
26	135104.0
27	164251.5
28	196634.5
29	230259.0
30	262314.0
31	296632.0
32	332401.5
33	367265.5
34	401853.5
35	434703.0
36	469383.0
37	499774.5
38	522415.0
39	541439.0
40	561646.5
41	581252.0
42	592521.5
43	601579.0
44	612136.5
45	622007.5
46	627812.5
47	629033.5
48	626100.0
49	616540.0
50	601434.5
51	581179.0
52	555499.5
53	527901.5
54	498880.0
55	463937.0
56	425971.5
57	386741.0
58	345040.0
59	300611.5
60	251764.5
61	204396.0
62	162297.0
63	126486.0
64	96611.0
65	72228.5
66	54137.0
67	41051.0
68	31083.5
69	22987.5
70	16891.0
71	12409.0
72	8939.0
73	6338.0
74	4431.5
75	3075.0
76	2135.5
77	1421.5
78	1008.0
79	719.0
80	511.5
81	369.5
82	277.0
83	195.5
84	135.0
85	101.0
86	72.0
87	51.5
88	33.0
89	21.0
90	16.5
91	12.5
92	10.5
93	7.0
94	3.5
95	3.5
96	3.5
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.8030067212869947
3	0.006600709674320331
4	8.772245126830022
5	4.051920790176837E-4
6	0.0753984034778551
7	0.026324414423923064
8	5.358992012814526E-4
9	3.267678056594223E-4
10-11	0.2993944665793325
12-13	0.30864199547949417
14-15	8.822730752804403E-5
16-17	1.7645461505608805E-4
18-19	0.4929815176861441
20-21	2.9085898829439637E-4
22-23	2.124718977313264E-4
24-25	0.17903706928922594
26-27	0.1641132934548171
28-29	1.3419792144439907E-4
30-31	1.1468723006517216E-4
32-33	1.1491802798562619E-4
34-35	4.081924219076873E-4
36-37	2.510992115782111E-4
38-39	0.0014978574660090972
40-41	0.001807498653730901
42-43	0.0012439452227040175
44-45	9.046292112903823E-4
46-47	0.0014669307643557297
48-49	0.002577044665876327
50-51	3.40863281476898E-4
52-53	0.00159435931792117
54-55	5.401233069737862E-4
56-57	0.010235416355633029
58-59	1.3280355482812707E-4
60-61	0.0026286097084115114
62-63	0.0015446443292108127
64-65	0.0015616076692025849
66-67	0.005024393392759173
68-69	0.0021610403518383795
70-71	0.0026294327870187858
72-73	0.003656759983662392
74-75	7.180090489213535E-4
76-77	3.273434547150142E-4
78-79	7.951775082230999E-4
80-81	5.091662454443046E-4
82-83	0.001587783505276888
84-85	2.6801188056058665E-4
86-87	4.3488424817252205E-4
88-89	1.2441672918952912E-4
90-91	3.020478973292212E-4
92-93	4.2455925866519075E-6
94-95	0.0
96-97	5.6685283567258965E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1174.0
20-21	3161.0
22-23	4159.0
24-25	5938.0
26-27	8237.0
28-29	13035.0
30-31	29080.0
32-33	36460.0
34-35	49782.0
36-37	73330.0
38-39	89239.0
40-41	90976.0
42-43	107482.0
44-45	132706.0
46-47	132291.0
48-49	121345.0
50-51	108227.0
52-53	104195.0
54-55	111608.0
56-57	119036.0
58-59	119870.0
60-61	119189.0
62-63	115786.0
64-65	119217.0
66-67	126919.0
68-69	129820.0
70-71	127375.0
72-73	119572.0
74-75	120625.0
76-77	126592.0
78-79	132114.0
80-81	132304.0
82-83	126536.0
84-85	125151.0
86-87	128983.0
88-89	136171.0
90-91	141596.0
92-93	142654.0
94-95	155153.0
96-97	246622.0
98-99	1263886.0
100-101	1.0003789E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.1961446107303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.81401340773934	73.88983502929622
2	7.496956448444323	12.474357456502421
3	1.9752526621226618	4.930002183620609
4	0.7655213690427569	2.547537060859418
5	0.3584720859344206	1.49117477501551
6	0.19838682605446017	0.9903011441574402
7	0.11643098003710567	0.6780626056635267
8	0.07851816418936325	0.5225926833973864
9	0.04322841423181061	0.3236793661550041
>10	0.1499649302387386	1.87411537528454
>50	0.0027301021609608	0.15025500021421856
>100	4.5553725080241237E-4	0.06437444417964323
>500	2.7650510138495694E-5	0.013697689890497132
>1k	4.1422043138877274E-5	0.05001518576355701
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.535356113188447E-6	0.0	6.535356113188447E-6	6.535356113188447E-6
3	0.0	6.535356113188447E-6	0.0	6.535356113188447E-6	6.535356113188447E-6
4	0.0	1.960606833956534E-5	0.0	1.960606833956534E-5	6.535356113188447E-6
5	1.3070712226376893E-5	1.960606833956534E-5	0.0	1.960606833956534E-5	6.535356113188447E-6
6	9.149498558463825E-5	1.960606833956534E-5	0.0	1.960606833956534E-5	1.3070712226376893E-5
7	9.149498558463825E-5	1.960606833956534E-5	0.0	1.960606833956534E-5	1.3070712226376893E-5
8	9.149498558463825E-5	3.2676780565942235E-5	0.0	1.960606833956534E-5	1.960606833956534E-5
9	9.149498558463825E-5	9.80303416978267E-5	0.0	3.2676780565942235E-5	2.6141424452753787E-5
10-11	1.372424783769574E-4	9.80303416978267E-5	0.0	4.5747492792319125E-5	2.6141424452753787E-5
12-13	1.4377783449014584E-4	9.80303416978267E-5	0.0	6.208588307529025E-5	2.6141424452753787E-5
14-15	1.568485467165227E-4	1.3397480032036318E-4	0.0	7.188891724507292E-5	5.881820501869602E-5
16-17	2.48343532301161E-4	1.372424783769574E-4	0.0	8.822730752804403E-5	9.80303416978267E-5
18-19	2.614142445275379E-4	1.535808686599285E-4	0.0	1.274394442071747E-4	1.1763641003739204E-4
20-21	2.646819225841321E-4	1.699192589428996E-4	0.0	1.3397480032036318E-4	1.2090408809398627E-4
22-23	2.875556689802917E-4	1.960606833956534E-4	0.0	1.7318693699949381E-4	1.437778344901458E-4
24-25	3.104294153764512E-4	2.123990736786245E-4	0.0	2.679496006407263E-4	1.927930053390592E-4
26-27	3.300354837160166E-4	2.222021078484072E-4	0.0	3.8231833262152416E-4	2.1893442979181298E-4
28-29	3.333031617726108E-4	2.254697859050014E-4	0.0	0.0010783337586760936	2.222021078484072E-4
30-31	3.496415520555819E-4	2.48343532301161E-4	0.0	0.00416302184410104	2.3200514201818988E-4
32-33	3.65979942338553E-4	2.48343532301161E-4	0.0	0.011178726631608838	2.679496006407263E-4
34-35	4.019244009610894E-4	2.9735870315007436E-4	0.0	0.02126278111425861	3.0062638120666857E-4
36-37	4.24798147357249E-4	3.463738739989877E-4	0.0	0.03656858513134595	3.0062638120666857E-4
38-39	4.24798147357249E-4	3.463738739989877E-4	0.0	0.05644587074960861	3.07161737319857E-4
40-41	4.313335034704375E-4	3.757829765083357E-4	0.0	0.07497360533049785	3.202324495462339E-4
42-43	4.313335034704375E-4	3.855860106781184E-4	0.0	0.09105384904699804	3.300354837160166E-4
44-45	4.4113653764022017E-4	4.149951131874664E-4	0.0	0.10876793179179531	3.431061959423934E-4
46-47	4.54207249866597E-4	4.378688595836259E-4	0.0	0.12437109451203274	3.627122642819588E-4
48-49	4.574749279231913E-4	4.444042156968144E-4	0.0	0.13744507441646622	4.117274351308721E-4
50-51	4.705456401495682E-4	5.097577768286988E-4	0.0	0.14720236109345658	4.149951131874664E-4
52-53	4.901517084891335E-4	5.097577768286988E-4	0.0	0.15437164674962428	4.3786885958362596E-4
54-55	4.901517084891335E-4	5.13025454885293E-4	0.0	0.16100830088256718	4.6727796209297397E-4
56-57	4.901517084891335E-4	5.162931329418872E-4	0.0	0.16714500027285112	4.705456401495682E-4
58-59	4.901517084891335E-4	5.195608109984816E-4	0.0	0.17285690151577782	4.738133182061624E-4
60-61	5.097577768286988E-4	5.326315232248584E-4	0.0	0.1781342015771775	4.770809962627566E-4
62-63	5.55505269621018E-4	5.391668793380468E-4	0.0	0.18305205705235178	4.96687064602322E-4
64-65	5.55505269621018E-4	6.404648990924678E-4	0.0	0.18800258930809205	4.999547426589162E-4
66-67	5.620406257342064E-4	6.404648990924678E-4	0.0	0.192681904285135	5.195608109984816E-4
68-69	5.751113379605834E-4	6.600709674320332E-4	0.0	0.1976977901020071	5.358992012814526E-4
70-71	6.012527624133371E-4	6.7314167965841E-4	0.0	0.20229868080569177	5.391668793380468E-4
72-73	6.43732577149062E-4	6.796770357715984E-4	0.0	0.2058473791751531	5.947174063001486E-4
74-75	6.470002552056562E-4	6.927477479979753E-4	0.0	0.20888305208972913	6.012527624133371E-4
76-77	6.502679332622504E-4	7.123538163375407E-4	0.0	0.21164424004755125	6.012527624133371E-4
78-79	6.666063235452216E-4	7.188891724507292E-4	0.0	0.21407212484360078	6.012527624133371E-4
80-81	6.666063235452216E-4	7.221568505073234E-4	0.0	0.21522561519757852	6.14323474639714E-4
82-83	6.7314167965841E-4	7.254245285639176E-4	0.0	0.2157909235013693	6.404648990924678E-4
84-85	6.829447138281927E-4	7.286922066205118E-4	0.0	0.2160882822045194	6.404648990924678E-4
86-87	6.894800699413812E-4	7.515659530166714E-4	0.0	0.21621898932678318	6.404648990924678E-4
88-89	6.927477479979754E-4	7.777073774694252E-4	0.0	0.2162483984292925	6.470002552056562E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21145	0.0	52.873234	1
GTATCAA	37370	0.0	38.59629	1
ATCAACG	45465	0.0	31.15814	3
TCAACGC	45925	0.0	30.91553	4
TATCAAC	47410	0.0	30.07525	2
CAACGCA	47510	0.0	28.490065	5
AACGCAG	49480	0.0	27.506973	6
ACGCAGA	55550	0.0	24.489782	7
CGCAGAG	56210	0.0	24.219278	8
GTGGTAT	11195	0.0	22.664421	1
TGGTATC	10530	0.0	22.441675	2
GCAGAGT	65025	0.0	20.670448	9
GTACATG	39355	0.0	20.549591	1
TACATGG	38795	0.0	20.433664	2
GAGTACT	41625	0.0	19.865454	12-13
ACATGGG	40095	0.0	19.273697	3
AGAGTAC	57935	0.0	19.240965	10-11
CAGAGTA	63085	0.0	18.974564	10-11
GTACTTT	45160	0.0	18.198551	14-15
AGTACTT	44395	0.0	17.713827	12-13
>>END_MODULE