FastQCFastQC Report
Fri 27 May 2016
SRR2139430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9128323
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113200.049.5860251
GTATCAA211750.039.023331
CTTATAC84350.035.687661
ATCAACG253300.032.2664643
TCAACGC257400.031.8191434
CAACGCA257350.031.8086645
TATCAAC272800.030.3060052
AACGCAG271050.030.2620476
TATACAC117700.028.1257763
ACGCAGA296750.027.568967
CGCAGAG300700.027.1925538
TTATACA133250.024.3287962
GCAGAGT352200.023.2407079
ACACATC134300.022.4765386
ACATCTC137900.022.0763288
TATGCCG83750.019.95050242-43
TACACAT168000.019.908985
GAGTACT226900.019.79485312-13
AGAGTAC325450.019.75581410-11
CAGAGTA350400.019.43207410-11
Produced by FastQC (version 0.11.5)