FastQCFastQC Report
Fri 27 May 2016
SRR2139429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9971591
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131250.052.4727481
GTATCAA250750.040.1789251
CTTATAC95450.034.78191
ATCAACG305950.032.3857043
CAACGCA314600.031.4817075
TCAACGC316950.031.3424114
TATCAAC324700.030.9100992
TATACAC122350.030.3385663
AACGCAG331750.030.0200396
ACGCAGA366550.027.0071937
CGCAGAG372500.026.5643628
ACATCTC144400.023.5504468
GCAGAGT423050.023.3599839
ACACATC147550.023.1632026
TATGCCG87700.021.97124942-43
GTGGTAT62250.021.6341321
TTATACA165400.021.5408362
TGCCGTC91500.021.30656244-45
TACACAT179800.021.1187845
CTCGTAT88650.021.1013938-39
Produced by FastQC (version 0.11.5)