##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9971591
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.881557015324837	32.0	30.0	33.0	18.0	33.0
2	31.436354339041785	33.0	31.0	33.0	28.0	33.0
3	31.079103023780256	33.0	31.0	33.0	28.0	33.0
4	32.101854959755165	33.0	33.0	33.0	30.0	34.0
5	32.21103282314728	33.0	33.0	33.0	31.0	34.0
6	35.721522974618594	38.0	36.0	38.0	31.0	38.0
7	36.80732362568822	38.0	37.0	38.0	35.0	38.0
8	36.71119192514013	38.0	38.0	38.0	34.0	38.0
9	37.06318640626155	38.0	38.0	38.0	36.0	38.0
10-11	37.18558738520262	38.0	38.0	38.0	36.0	38.0
12-13	37.23875066676922	38.0	38.0	38.0	36.0	38.0
14-15	37.28764361675083	38.0	38.0	38.0	37.0	38.0
16-17	37.29959988330849	38.0	38.0	38.0	37.0	38.0
18-19	37.326336088192946	38.0	38.0	38.0	37.0	38.0
20-21	37.29944134479875	38.0	38.0	38.0	37.0	38.0
22-23	37.32818921174197	38.0	38.0	38.0	37.0	38.0
24-25	37.35303994315217	38.0	38.0	38.0	37.0	38.0
26-27	37.330709117647956	38.0	38.0	38.0	37.0	38.0
28-29	37.313483785488344	38.0	38.0	38.0	37.0	38.0
30-31	37.3089295063768	38.0	38.0	38.0	37.0	38.0
32-33	37.24131228003924	38.0	38.0	38.0	37.0	38.0
34-35	37.249231732342125	38.0	38.0	38.0	37.0	38.0
36-37	37.24795083509437	38.0	38.0	38.0	37.0	38.0
38-39	37.21641811051718	38.0	38.0	38.0	37.0	38.0
40-41	37.22480013671152	38.0	38.0	38.0	37.0	38.0
42-43	37.19389443080716	38.0	38.0	38.0	37.0	38.0
44-45	37.1617418299724	38.0	38.0	38.0	36.0	38.0
46-47	37.12604049112872	38.0	38.0	38.0	36.0	38.0
48-49	37.0807026456357	38.0	38.0	38.0	36.0	38.0
50-51	37.05499128443282	38.0	38.0	38.0	36.0	38.0
52-53	37.028161945870075	38.0	38.0	38.0	36.0	38.0
54-55	37.0057875708853	38.0	38.0	38.0	36.0	38.0
56-57	36.97695088445951	38.0	38.0	38.0	36.0	38.0
58-59	36.98386702976835	38.0	38.0	38.0	36.0	38.0
60-61	37.00617174688642	38.0	38.0	38.0	36.0	38.0
62-63	37.015316556262505	38.0	38.0	38.0	36.0	38.0
64-65	37.014034084344644	38.0	38.0	38.0	36.0	38.0
66-67	37.02061900101184	38.0	38.0	38.0	36.0	38.0
68-69	37.02154557160256	38.0	38.0	38.0	36.0	38.0
70-71	37.015000825401685	38.0	38.0	38.0	36.0	38.0
72-73	36.99333691865812	38.0	38.0	38.0	36.0	38.0
74-75	36.993996124744015	38.0	38.0	38.0	36.0	38.0
76-77	36.99367324493768	38.0	38.0	38.0	36.0	38.0
78-79	36.971602267526166	38.0	38.0	38.0	36.0	38.0
80-81	36.953863315965506	38.0	38.0	38.0	36.0	38.0
82-83	36.95271914281202	38.0	38.0	38.0	36.0	38.0
84-85	36.90249820380696	38.0	38.0	38.0	35.5	38.0
86-87	36.89254238025754	38.0	38.0	38.0	35.0	38.0
88-89	36.89903329359089	38.0	38.0	38.0	35.0	38.0
90-91	36.88079854206346	38.0	38.0	38.0	35.0	38.0
92-93	36.888448332590414	38.0	38.0	38.0	35.0	38.0
94-95	36.83674207806541	38.0	38.0	38.0	35.0	38.0
96-97	36.81210319333054	38.0	38.0	38.0	35.0	38.0
98-99	36.89117199926025	38.0	38.0	38.0	35.0	38.0
100	35.28174856008782	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	38.0
19	61.0
20	103.0
21	240.0
22	694.0
23	2079.0
24	4714.0
25	9771.0
26	17493.0
27	29002.0
28	45266.0
29	66040.0
30	90642.0
31	118809.0
32	154001.0
33	209055.0
34	307437.0
35	534089.0
36	1441062.0
37	6940989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.745362600612076	20.520787505223588	14.619131490651792	25.114718403512537
2	13.357326791644322	20.49839093665448	44.168244774593234	21.97603749710796
3	16.71533659974622	26.95925855763639	31.466192305721325	24.85921253689607
4	11.284227361511318	18.481554247461613	41.08813728922496	29.146081101802107
5	12.230039777330392	38.02215142584409	36.76252927738686	12.985279519438661
6	28.034606026460455	39.715308279809435	19.32518469029614	12.924901003433966
7	24.95993868982392	33.6451825992462	24.12516718746286	17.26971152346702
8	23.466405711987186	37.23192216768618	21.862970512930183	17.438701607396453
9	25.16672615232614	16.92071004516732	22.020608346250864	35.89195545625567
10-11	23.339620527957873	27.36462516362735	29.780824343878525	19.51492996453625
12-13	24.390290375928977	24.763646042040836	29.6335659976427	21.212497584387485
14-15	21.5952298885905	26.066687853523074	27.675418095266846	24.662664162619585
16-17	20.84145849945109	29.2972004166637	28.162983219026934	21.698357864858274
18-19	20.713640380958264	28.34242298947079	30.21531869889168	20.728617930679267
20-21	21.95897776305351	27.545274153080413	29.849428550755253	20.64631953311082
22-23	21.464434919848205	27.516017973064873	29.786204049024267	21.23334305806265
24-25	21.253327985588772	27.607415371967374	30.097160555248735	21.042096087195116
26-27	21.219533638486148	27.846313478539958	29.868984433134106	21.06516844983979
28-29	20.95446078423995	28.084125113568355	29.901760554017898	21.059653548173802
30-31	21.772922322445048	27.612407214436587	29.801655417375354	20.81301504574301
32-33	21.165565482028484	28.03711056543096	29.67213191455601	21.125192037984544
34-35	21.23524344346384	28.034537126048708	29.870251246041967	20.85996818444548
36-37	21.221154873710667	27.78944955551932	29.948171299522365	21.04122427124765
38-39	21.01768954101522	27.74166808454658	30.157883091550442	21.08275928288776
40-41	21.34694487756998	27.668708815004393	30.023243787097815	20.96110252032781
42-43	21.449504113512397	27.568119154680527	29.827657837292133	21.154718894514946
44-45	21.311836415891936	28.08872704426862	29.55420578010792	21.045230759731528
46-47	21.528097649342357	27.539876661312316	29.205322870279467	21.726702819065867
48-49	21.50306064086547	27.61958492638078	29.450582260517923	21.42677217223582
50-51	21.15581551964025	27.81960994393577	29.371858951872753	21.65271558455122
52-53	21.36359861648272	27.86021135707022	29.336625752207386	21.439564274239682
54-55	21.4595996835142	27.71801585713757	29.164962179491592	21.65742227985664
56-57	21.688075297982	28.027339266373325	28.7755791841334	21.50900625151128
58-59	21.274467007600386	28.230858974572428	29.357112322779205	21.137561695047978
60-61	21.49675237790803	28.528612196840225	28.759479321615245	21.215156103636502
62-63	21.29161365211674	28.596944308933992	28.747553028993778	21.36388900995549
64-65	21.16323411844116	28.610653922078395	28.83255075269754	21.393561206782906
66-67	21.47460651423092	28.735335792559276	28.44264444286111	21.347413250348694
68-69	21.441565068383376	28.463921744270632	28.787390232992376	21.307122954353616
70-71	21.441158158039215	27.92241667475766	28.87870183333117	21.757723333871954
72-73	21.611632337963886	27.854285998328336	29.04484618217438	21.4892354815334
74-75	21.36300190565344	28.068722925631757	28.91907898193211	21.649196186782692
76-77	21.373151809699145	28.16715432578876	28.693029351646427	21.766664512865667
78-79	21.557080015648015	28.205405433047197	28.643731338685953	21.59378321261884
80-81	21.46123802279797	28.036208181667316	28.705937508495943	21.79661628703877
82-83	21.642152974560577	27.919834221189987	28.891334634624165	21.546678169625267
84-85	22.06601371491089	27.892312718842692	28.51565615301437	21.526017413232047
86-87	21.312731256077974	28.19271939244511	28.665667679490525	21.828881671986395
88-89	21.685283630270767	28.006251386251563	28.68365137508471	21.624813608392962
90-91	21.84490942624431	27.961400178828978	28.69548056176687	21.49820983315984
92-93	21.793942009811673	28.11765005350413	28.51656383435178	21.571844102332424
94-95	21.54029128327756	28.226805998813642	28.544085318061	21.688817399847792
96-97	21.50322937774191	28.383886082301157	28.71283661267734	21.400047927279594
98-99	22.52177285320968	30.29191377027871	28.928059043041326	18.258254333470283
100	21.606240049907687	31.359833086795447	22.78581647341754	24.24811038987933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1694.0
1	1739.5
2	2796.0
3	4202.5
4	5603.0
5	7889.5
6	9549.0
7	11299.5
8	13656.0
9	15048.0
10	15283.5
11	15738.5
12	17834.5
13	22067.5
14	29918.0
15	38936.0
16	45552.5
17	48615.0
18	47204.5
19	43208.5
20	39584.0
21	37236.0
22	38723.0
23	45825.5
24	57264.0
25	73897.0
26	99304.5
27	128248.5
28	148545.5
29	169524.0
30	190186.0
31	207282.5
32	235070.5
33	266989.5
34	298194.0
35	324197.0
36	354532.5
37	383357.0
38	387034.5
39	387348.5
40	399717.0
41	406911.0
42	408331.0
43	426071.0
44	442121.5
45	437759.0
46	425621.5
47	415287.0
48	403775.0
49	387491.5
50	370632.5
51	353015.0
52	333036.5
53	311264.5
54	289967.5
55	265120.0
56	238050.0
57	210451.5
58	182764.0
59	160337.5
60	135487.5
61	103425.5
62	74333.5
63	53126.0
64	38085.5
65	27138.0
66	19671.5
67	14505.0
68	10798.5
69	8096.5
70	6100.5
71	4249.5
72	2819.5
73	1842.5
74	1217.0
75	785.5
76	476.5
77	271.5
78	176.5
79	110.5
80	71.5
81	47.5
82	29.0
83	15.0
84	8.5
85	8.0
86	7.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0026274643635102964
3	0.0
4	0.0
5	0.005866666613181387
6	3.309401679230526E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.767069032159414E-5
44-45	4.677654754880236E-5
46-47	0.0
48-49	0.0
50-51	1.601026706406284E-5
52-53	0.0011240897763712134
54-55	0.0021410086741746872
56-57	0.002574304062038651
58-59	0.0017035002188363795
60-61	0.004115109168285276
62-63	0.0016322689817737817
64-65	0.0015954998304922876
66-67	2.3426223451660904E-4
68-69	2.2524403458500864E-4
70-71	2.2199062498539534E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	8.522878083394658E-5
80-81	3.3214793426128084E-4
82-83	1.2427568248631897E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.236371010582092E-5
92-93	2.3557072078424107E-4
94-95	0.0
96-97	0.0
98-99	7.244757765728297E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	104.0
20-21	232.0
22-23	541.0
24-25	827.0
26-27	2228.0
28-29	6048.0
30-31	18019.0
32-33	23236.0
34-35	31698.0
36-37	51356.0
38-39	63304.0
40-41	61739.0
42-43	71365.0
44-45	84534.0
46-47	87132.0
48-49	81398.0
50-51	74042.0
52-53	70636.0
54-55	75428.0
56-57	78816.0
58-59	77516.0
60-61	78363.0
62-63	76276.0
64-65	83123.0
66-67	91725.0
68-69	95250.0
70-71	111421.0
72-73	81198.0
74-75	78465.0
76-77	81522.0
78-79	83795.0
80-81	83997.0
82-83	78359.0
84-85	78520.0
86-87	79861.0
88-89	83508.0
90-91	84327.0
92-93	83638.0
94-95	98145.0
96-97	284218.0
98-99	652400.0
100-101	6543281.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.99335428732348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.44097225350723	66.5538040165498
2	7.978137876765477	12.285271921978385
3	2.616435318851676	6.043443944226399
4	1.19025298712682	3.6656627971760134
5	0.614271889168688	2.3647426595754153
6	0.3495232798782506	1.6146581831600084
7	0.2313419868159458	1.2468256886717404
8	0.1448244849385215	0.8920418302680585
9	0.10641069066837706	0.7373624405930227
>10	0.3221385668602553	3.588527116979413
>50	0.0036013983802488086	0.18155896928730195
>100	0.0016806558037271537	0.2329267699305301
>500	1.3620422157888112E-4	0.06411637546596846
>1k	2.451663118889623E-4	0.40061539038741867
>5k	2.7240701320965227E-5	0.12844189575053608
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.00569798741244E-5	0.0
4	0.0	0.0	0.0	4.01139597482488E-5	2.00569798741244E-5
5	0.0	0.0	0.0	4.01139597482488E-5	2.00569798741244E-5
6	0.0	0.0	0.0	4.01139597482488E-5	3.00854698111866E-5
7	0.0	0.0	0.0	1.203418792447464E-4	3.00854698111866E-5
8	0.0	0.0	0.0	1.203418792447464E-4	3.00854698111866E-5
9	0.0	0.0	0.0	2.105982886783062E-4	5.0142449685311E-5
10-11	0.0	0.0	0.0	2.105982886783062E-4	5.0142449685311E-5
12-13	0.0	0.0	0.0	2.206267786153684E-4	5.0142449685311E-5
14-15	0.0	5.0142449685311E-6	0.0	2.206267786153684E-4	6.51851845909043E-5
16-17	5.0142449685311E-6	1.00284899370622E-5	0.0	2.306552685524306E-4	7.01994295594354E-5
18-19	1.00284899370622E-5	1.00284899370622E-5	0.0	2.757834732692105E-4	7.01994295594354E-5
20-21	1.00284899370622E-5	1.00284899370622E-5	0.0	3.660398827027703E-4	7.01994295594354E-5
22-23	1.00284899370622E-5	1.00284899370622E-5	0.0	3.760683726398325E-4	8.52421644650287E-5
24-25	1.00284899370622E-5	1.00284899370622E-5	0.0	4.7133902704192337E-4	1.00284899370622E-4
26-27	1.00284899370622E-5	1.00284899370622E-5	0.0	6.51851845909043E-4	1.00284899370622E-4
28-29	1.00284899370622E-5	1.00284899370622E-5	0.0	0.001007863238674751	1.00284899370622E-4
30-31	1.00284899370622E-5	1.00284899370622E-5	0.0	0.0020959543968459998	1.00284899370622E-4
32-33	1.00284899370622E-5	1.00284899370622E-5	0.0	0.004116695119164033	1.00284899370622E-4
34-35	1.00284899370622E-5	1.00284899370622E-5	0.0	0.006714074012863143	1.103133893076842E-4
36-37	1.00284899370622E-5	1.00284899370622E-5	0.0	0.010514871699009717	1.103133893076842E-4
38-39	1.00284899370622E-5	1.00284899370622E-5	0.0	0.016973219218477775	1.103133893076842E-4
40-41	1.00284899370622E-5	1.00284899370622E-5	0.0	0.023877834540145096	1.103133893076842E-4
42-43	1.00284899370622E-5	1.00284899370622E-5	0.0	0.030962962680679544	1.153276342762153E-4
44-45	1.00284899370622E-5	1.00284899370622E-5	0.0	0.03858461503284681	1.203418792447464E-4
46-47	1.00284899370622E-5	1.00284899370622E-5	0.0	0.04706871751960144	1.203418792447464E-4
48-49	1.00284899370622E-5	1.00284899370622E-5	0.0	0.05556786274126165	1.403988591188708E-4
50-51	1.00284899370622E-5	1.00284899370622E-5	0.0	0.06330985697267366	1.403988591188708E-4
52-53	1.00284899370622E-5	1.00284899370622E-5	0.0	0.07139783410691433	1.454131040874019E-4
54-55	1.00284899370622E-5	1.00284899370622E-5	0.0	0.07841777706285788	1.50427349055933E-4
56-57	1.00284899370622E-5	1.00284899370622E-5	0.0	0.08335680835686102	1.50427349055933E-4
58-59	1.00284899370622E-5	1.00284899370622E-5	0.0	0.08885743508733962	1.604558389929952E-4
60-61	1.00284899370622E-5	1.00284899370622E-5	0.0	0.09437310455272382	1.704843289300574E-4
62-63	1.00284899370622E-5	1.00284899370622E-5	0.0	0.09943247772597172	1.704843289300574E-4
64-65	1.00284899370622E-5	1.00284899370622E-5	0.0	0.10464227824827553	1.704843289300574E-4
66-67	1.00284899370622E-5	1.00284899370622E-5	0.0	0.10938575398850595	1.754985738985885E-4
68-69	1.00284899370622E-5	1.00284899370622E-5	0.0	0.11446518414162796	1.805128188671196E-4
70-71	1.00284899370622E-5	1.00284899370622E-5	0.0	0.11911338922745629	2.00569798741244E-4
72-73	1.00284899370622E-5	1.00284899370622E-5	0.0	0.12294928662838257	2.105982886783062E-4
74-75	1.00284899370622E-5	1.00284899370622E-5	0.0	0.12561185070667258	2.105982886783062E-4
76-77	1.00284899370622E-5	1.00284899370622E-5	0.0	0.12859031221798006	2.105982886783062E-4
78-79	1.00284899370622E-5	1.00284899370622E-5	0.0	0.13058598171545543	2.105982886783062E-4
80-81	1.00284899370622E-5	1.00284899370622E-5	0.0	0.13126290478620714	2.256410235838995E-4
82-83	1.00284899370622E-5	1.00284899370622E-5	0.0	0.13178438626293437	2.50712248426555E-4
84-85	1.00284899370622E-5	1.00284899370622E-5	0.0	0.13202005577645534	2.50712248426555E-4
86-87	1.00284899370622E-5	1.00284899370622E-5	0.0	0.1321454119006686	2.557264933950861E-4
88	1.00284899370622E-5	1.00284899370622E-5	0.0	0.13215544039060567	2.607407383636172E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13125	0.0	52.472748	1
GTATCAA	25075	0.0	40.178925	1
CTTATAC	9545	0.0	34.7819	1
ATCAACG	30595	0.0	32.385704	3
CAACGCA	31460	0.0	31.481707	5
TCAACGC	31695	0.0	31.342411	4
TATCAAC	32470	0.0	30.910099	2
TATACAC	12235	0.0	30.338566	3
AACGCAG	33175	0.0	30.020039	6
ACGCAGA	36655	0.0	27.007193	7
CGCAGAG	37250	0.0	26.564362	8
ACATCTC	14440	0.0	23.550446	8
GCAGAGT	42305	0.0	23.359983	9
ACACATC	14755	0.0	23.163202	6
TATGCCG	8770	0.0	21.971249	42-43
GTGGTAT	6225	0.0	21.634132	1
TTATACA	16540	0.0	21.540836	2
TGCCGTC	9150	0.0	21.306562	44-45
TACACAT	17980	0.0	21.118784	5
CTCGTAT	8865	0.0	21.10139	38-39
>>END_MODULE