FastQCFastQC Report
Fri 27 May 2016
SRR2139428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9035208
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125150.053.616461
CTTATAC115750.048.624041
TATACAC131000.045.211763
TTATACA141700.040.5511742
GTATCAA245150.040.026811
TACACAT186700.031.7683945
ATCAACG306900.031.4236643
TATCAAC315850.030.9472452
CAACGCA313550.030.810915
TCAACGC316900.030.64464
ATACACA207750.029.3802574
AACGCAG333950.029.1545286
ACACATC198900.028.6970166
ACATCTC205150.027.9867348
ACGCAGA361650.026.7234697
CGCAGAG374400.025.7796848
CACATCT222050.025.7429547
CATCTCC245550.023.3992589
GCAGAGT430450.022.4032889
GAGTACT245150.020.67302112-13
Produced by FastQC (version 0.11.5)