##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9035208
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.172277605562595	32.0	30.0	33.0	18.0	33.0
2	31.459714375142223	33.0	31.0	33.0	28.0	33.0
3	31.13989362502778	33.0	31.0	33.0	28.0	33.0
4	32.079463582908105	33.0	33.0	33.0	29.0	34.0
5	32.21333310754993	33.0	33.0	33.0	31.0	34.0
6	35.73048998982647	38.0	36.0	38.0	31.0	38.0
7	36.80546147913805	38.0	37.0	38.0	35.0	38.0
8	36.719470763705715	38.0	38.0	38.0	34.0	38.0
9	37.06267669764769	38.0	38.0	38.0	36.0	38.0
10-11	37.179182925285176	38.0	38.0	38.0	36.0	38.0
12-13	37.2305343717599	38.0	38.0	38.0	36.0	38.0
14-15	37.27656053961348	38.0	38.0	38.0	37.0	38.0
16-17	37.288702761463824	38.0	38.0	38.0	37.0	38.0
18-19	37.31451223923124	38.0	38.0	38.0	37.0	38.0
20-21	37.29156278808076	38.0	38.0	38.0	37.0	38.0
22-23	37.319153990382276	38.0	38.0	38.0	37.0	38.0
24-25	37.34153326782726	38.0	38.0	38.0	37.0	38.0
26-27	37.31933376918796	38.0	38.0	38.0	37.0	38.0
28-29	37.300694755546154	38.0	38.0	38.0	37.0	38.0
30-31	37.29638458971719	38.0	38.0	38.0	37.0	38.0
32-33	37.22742909009327	38.0	38.0	38.0	37.0	38.0
34-35	37.23452220836772	38.0	38.0	38.0	37.0	38.0
36-37	37.23159352689542	38.0	38.0	38.0	37.0	38.0
38-39	37.18525882022968	38.0	38.0	38.0	36.5	38.0
40-41	37.22280249670905	38.0	38.0	38.0	37.0	38.0
42-43	37.199435728521436	38.0	38.0	38.0	37.0	38.0
44-45	37.16833124439458	38.0	38.0	38.0	36.0	38.0
46-47	37.13384221552302	38.0	38.0	38.0	36.0	38.0
48-49	37.08753472555476	38.0	38.0	38.0	36.0	38.0
50-51	37.06220120602936	38.0	38.0	38.0	36.0	38.0
52-53	37.03388449815364	38.0	38.0	38.0	36.0	38.0
54-55	37.01001375333564	38.0	38.0	38.0	36.0	38.0
56-57	36.98182799176978	38.0	38.0	38.0	36.0	38.0
58-59	36.98925634343259	38.0	38.0	38.0	36.0	38.0
60-61	37.01323405635907	38.0	38.0	38.0	36.0	38.0
62-63	37.02494777092774	38.0	38.0	38.0	36.0	38.0
64-65	37.02688132687752	38.0	38.0	38.0	36.0	38.0
66-67	37.03175779460594	38.0	38.0	38.0	36.0	38.0
68-69	37.0353376331508	38.0	38.0	38.0	36.0	38.0
70-71	37.024888542485996	38.0	38.0	38.0	36.0	38.0
72-73	37.001853051048386	38.0	38.0	38.0	36.0	38.0
74-75	37.00231767833719	38.0	38.0	38.0	36.0	38.0
76-77	37.00272380422762	38.0	38.0	38.0	36.0	38.0
78-79	36.98129337065278	38.0	38.0	38.0	36.0	38.0
80-81	36.96227575358152	38.0	38.0	38.0	36.0	38.0
82-83	36.96672233926186	38.0	38.0	38.0	36.0	38.0
84-85	36.92075371289829	38.0	38.0	38.0	35.5	38.0
86-87	36.90965150130982	38.0	38.0	38.0	35.0	38.0
88-89	36.919027878067766	38.0	38.0	38.0	35.0	38.0
90-91	36.90092016334825	38.0	38.0	38.0	35.0	38.0
92-93	36.90773273503409	38.0	38.0	38.0	35.0	38.0
94-95	36.8594736176842	38.0	38.0	38.0	35.0	38.0
96-97	36.83831705470608	38.0	38.0	38.0	35.0	38.0
98-99	36.92439579339782	38.0	38.0	38.0	35.0	38.0
100	35.363850884360644	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	37.0
19	64.0
20	112.0
21	249.0
22	684.0
23	2056.0
24	4652.0
25	9219.0
26	15986.0
27	26042.0
28	40346.0
29	58963.0
30	80941.0
31	107815.0
32	141788.0
33	189394.0
34	277760.0
35	481085.0
36	1305415.0
37	6292591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.37361397767489	19.779754932039197	14.101125286767054	25.74550580351886
2	14.18372185274484	19.868956049064394	42.69222834262905	23.255093755561713
3	17.220411527880707	26.21341976853217	30.886217561344466	25.679951142242658
4	12.005158043954273	18.165979134071954	38.80195121130582	31.026911610667955
5	13.138044646342713	37.09230629087231	35.75883541366596	14.010813649119013
6	29.542983898262502	37.91075855318337	18.544346793985373	14.001910754568755
7	26.13047757173936	32.70753700412874	22.87452596553394	18.287459458597965
8	24.716464745471274	35.69323473239354	21.169960890773073	18.42033963136211
9	25.794691168150198	16.22551467547842	21.000069948583363	36.979724207788024
10-11	24.24764875363135	26.389757712274033	28.69273181093341	20.669861723161215
12-13	25.33081695518244	23.806867534206187	28.56661960632229	22.295695904289087
14-15	22.56835149782938	24.954837785693478	26.188223890363123	26.28858682611402
16-17	22.05228700877722	28.297383967253438	26.841656550684835	22.80867247328451
18-19	22.147265453103017	26.97470274065633	28.884503821052043	21.99352798518861
20-21	22.88566828231949	26.11606081761053	28.631753181153723	22.366517718916253
22-23	22.680488937368665	26.45487755812454	28.498272492873305	22.366361011633487
24-25	22.56850525102136	26.66430301707362	28.591760375078646	22.175431356826373
26-27	22.523527202857803	26.85040919088652	28.659818477865702	21.96624512838998
28-29	21.90633815299352	26.526094213589264	28.841069381034785	22.726498252382434
30-31	22.641769352206605	26.276283667646872	28.949977088598995	22.131969891547527
32-33	22.228533588943687	26.822241856350736	28.574501711763773	22.374722842941804
34-35	22.086412976828772	26.911882557399586	29.11758640826867	21.884118057502974
36-37	22.563952182610887	26.623514323182874	28.595578210852945	22.21695528335329
38-39	22.29220131477331	26.293568512336453	28.96332019536059	22.450909977529644
40-41	22.34334296384913	26.119648832584698	29.131623129911016	22.405385073655157
42-43	22.68596191636233	26.137298363797118	28.829307659600744	22.347432060239804
44-45	22.327879767654295	26.827431561739772	28.498446231019813	22.346242439586117
46-47	22.75005087660522	26.29820889325969	28.04028670603762	22.911453524097467
48-49	22.770051496373657	26.184930012059297	27.985707292080004	23.05931119948704
50-51	22.259151979198478	26.40116156701446	28.29338845034308	23.046298003443983
52-53	22.38563008398312	26.6036004593928	28.395951466315637	22.614817990308435
54-55	22.462770841150363	26.577957731950058	27.94967261987349	23.009598807026094
56-57	23.007117848237755	26.817722418832673	27.44497256046951	22.730187172460063
58-59	22.27891350822677	27.257149830030595	28.27130737273861	22.192629289004024
60-61	22.75442444867359	27.87218568884247	27.152603457471276	22.220786405012657
62-63	22.381955520063446	28.18833703168147	27.093934282947817	22.335773165307263
64-65	22.20463529990324	28.10901080968975	27.21949745211204	22.466856438294972
66-67	22.40921369475081	27.936600307200887	27.053357720294468	22.60082827775383
68-69	22.390270374835037	27.651196923578265	27.314038186526247	22.644494515060458
70-71	22.530000935548973	27.190015789775497	27.404566598215556	22.875416676459974
72-73	22.819465770376517	26.880154420450648	27.488701428082756	22.81167838109008
74-75	22.586376964249858	27.0591909957698	27.4963001824725	22.85813185750784
76-77	22.73648673803135	26.969164508031717	27.33581537928094	22.958533374655985
78-79	22.70511569531226	26.925462649706073	27.373413034325942	22.996008620655726
80-81	22.60502096397414	26.960889871266787	27.438842507096915	22.995246657662157
82-83	22.957737966723624	26.807421968383643	27.552369503918545	22.68247056097419
84-85	23.07281932714642	26.8633134595692	27.169376563230323	22.894490650054056
86-87	22.681072707283917	27.054571406679795	27.409616779560768	22.85473910647552
88-89	22.900364346994692	26.759198565026193	27.398622579240513	22.94181450873861
90-91	22.987696012959507	26.77662590193148	27.339278029560667	22.89640005554834
92-93	23.003814935689494	26.885088238363597	27.2466282301954	22.864468595751507
94-95	22.74147206426387	27.021890425646493	27.318218645284105	22.918418864805535
96-97	22.896110355268913	27.044503142418748	27.340685581569605	22.718700920742734
98-99	24.02763629449689	28.892199854336386	27.52917372071296	19.550990130453762
100	23.039094952535887	29.711889089614342	21.218559716762172	26.030456241087595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1595.0
1	1743.0
2	2872.5
3	4279.0
4	5751.5
5	8019.0
6	9576.0
7	11263.5
8	13546.0
9	14741.0
10	14576.5
11	14634.0
12	16541.0
13	20478.5
14	27791.5
15	36142.5
16	42112.5
17	45081.0
18	43667.5
19	38773.5
20	33202.5
21	29138.5
22	28324.5
23	31500.5
24	38611.0
25	48814.0
26	62765.0
27	78951.0
28	93266.5
29	109368.5
30	125732.5
31	141970.0
32	161393.5
33	180453.5
34	200597.0
35	223276.5
36	250117.0
37	276445.0
38	288259.0
39	291537.0
40	301399.0
41	309907.5
42	319710.0
43	356777.0
44	396306.0
45	414484.0
46	410581.5
47	398292.5
48	396904.5
49	395329.5
50	396378.5
51	394406.5
52	382262.0
53	370936.5
54	358482.0
55	332852.0
56	303305.5
57	273298.5
58	240834.5
59	208736.5
60	170423.0
61	132904.0
62	103309.5
63	78244.5
64	57626.0
65	41463.5
66	29753.0
67	22230.0
68	16887.0
69	12441.5
70	8968.5
71	6494.0
72	4632.5
73	3126.5
74	2102.5
75	1333.5
76	790.0
77	464.5
78	302.0
79	205.5
80	123.5
81	75.5
82	51.5
83	34.5
84	22.0
85	13.0
86	6.5
87	3.0
88	4.0
89	4.0
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0024791902964491795
3	0.0
4	0.0
5	0.0056999241190684264
6	3.7630566999675047E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.426400373467962E-5
44-45	1.728522317959952E-5
46-47	0.0
48-49	0.0
50-51	5.93217307542064E-6
52-53	9.571588844265343E-4
54-55	0.0022560789564898885
56-57	0.002526176053652814
58-59	0.0016957943930401651
60-61	0.00422455684646819
62-63	0.001829715176008574
64-65	0.0016902911763951786
66-67	1.345291307457872E-4
68-69	9.737145750466166E-5
70-71	1.8434428507368931E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	6.905137429152427E-5
80-81	3.142072582295594E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	5.906252853919836E-5
92-93	2.5399472064502706E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	93.0
20-21	294.0
22-23	612.0
24-25	863.0
26-27	2413.0
28-29	6831.0
30-31	18957.0
32-33	24092.0
34-35	32683.0
36-37	52751.0
38-39	63803.0
40-41	62525.0
42-43	70866.0
44-45	84307.0
46-47	86812.0
48-49	80395.0
50-51	72005.0
52-53	68189.0
54-55	73577.0
56-57	76409.0
58-59	76429.0
60-61	81063.0
62-63	80612.0
64-65	88969.0
66-67	99724.0
68-69	102637.0
70-71	132479.0
72-73	80826.0
74-75	74877.0
76-77	78192.0
78-79	79776.0
80-81	80206.0
82-83	75747.0
84-85	75758.0
86-87	76776.0
88-89	79951.0
90-91	79943.0
92-93	79356.0
94-95	95241.0
96-97	272673.0
98-99	589418.0
100-101	5676078.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.37288947840203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.01112018798808	67.32301785697157
2	7.637179185285478	11.818212420596915
3	2.4345738051338435	5.6511002985490055
4	1.1166462688373635	3.455925933808933
5	0.5967295246184698	2.3085343778402123
6	0.3481415310919162	1.6162029724809894
7	0.22262960380457775	1.205784701085442
8	0.15561989702121007	0.9632608874289914
9	0.1036824220286766	0.7219987722430048
>10	0.3680245015842785	4.321500125552182
>50	0.0036541145007487675	0.18214785943611603
>100	0.001773728012395907	0.2878713666069975
>500	1.287029901173912E-4	0.056885764423669986
>1k	9.652710287373799E-5	0.08755666297591476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.2135627646867677E-5	0.0	0.0	0.0
5	0.0	2.2135627646867677E-5	0.0	0.0	0.0
6	0.0	2.2135627646867677E-5	0.0	0.0	0.0
7	0.0	2.2135627646867677E-5	0.0	0.0	0.0
8	0.0	3.320344147030151E-5	0.0	0.0	0.0
9	0.0	9.961032441090454E-5	0.0	0.0	0.0
10-11	0.0	9.961032441090454E-5	0.0	0.0	0.0
12-13	0.0	9.961032441090454E-5	0.0	0.0	0.0
14-15	0.0	1.7708502117494141E-4	0.0	0.0	0.0
16-17	0.0	1.7708502117494141E-4	0.0	0.0	0.0
18-19	0.0	1.826189280866583E-4	0.0	0.0	0.0
20-21	0.0	1.8815283499837524E-4	0.0	0.0	0.0
22-23	0.0	1.8815283499837524E-4	0.0	3.320344147030152E-5	0.0
24-25	0.0	2.102884626452429E-4	0.0	7.194078985231995E-5	0.0
26-27	0.0	2.2135627646867675E-4	0.0	1.2174595205777223E-4	0.0
28-29	0.0	2.324240902921106E-4	0.0	4.150430183787689E-4	0.0
30-31	0.0	2.7116143867412905E-4	0.0	0.001499688773075285	0.0
32-33	0.0	2.7669534558584594E-4	0.0	0.0036966498170269016	0.0
34-35	0.0	3.2096660087958134E-4	0.0	0.006004288999212857	0.0
36-37	0.0	3.652378561733167E-4	0.0	0.010010837603295906	0.0
38-39	0.0	3.652378561733167E-4	0.0	0.016064931764714217	0.0
40-41	0.0	3.9844129764361817E-4	0.0	0.022495331596129278	0.0
42-43	0.0	4.316447391139197E-4	0.0	0.028859324544603732	0.0
44-45	0.0	5.201872497013904E-4	0.0	0.036402039665273896	0.0
46-47	0.0	5.644585049951257E-4	0.0	0.04421591622461818	0.0
48-49	0.0	5.644585049951257E-4	0.0	0.05221241171204913	0.0
50-51	0.0	6.087297602888611E-4	0.0	0.06041366175521361	0.0
52-53	0.0	6.087297602888611E-4	0.0	0.06821093659382274	0.0
54-55	0.0	6.087297602888611E-4	0.0	0.07600821143243189	0.0
56-57	0.0	6.087297602888611E-4	0.0	0.08328529902133963	0.0
58-59	0.0	6.197975741122949E-4	0.0	0.09032996251995526	0.0
60-61	0.0	6.474671086708795E-4	0.0	0.09712560020754364	0.0
62-63	0.0	6.696027363177473E-4	0.0	0.10343978799381265	0.0
64-65	0.0	7.802808745520856E-4	0.0	0.10951048387596611	0.0
66-67	0.0	7.968825952872363E-4	0.0	0.11597962105576318	0.0
68-69	0.0	8.19018222934104E-4	0.0	0.12239341916644308	0.0
70-71	0.0	8.245521298458209E-4	0.0	0.12748461352522267	0.0
72-73	0.0	8.300860367575378E-4	0.0	0.131817662637097	0.0
74-75	0.0	8.411538505809716E-4	0.0	0.13528742227074353	0.0
76-77	0.0	8.90959012786424E-4	0.0	0.1387129106490963	0.0
78-79	0.0	9.075607335215747E-4	0.0	0.14112569406260486	0.0
80-81	0.0	9.075607335215747E-4	0.0	0.14186723758877495	0.0
82-83	0.0	9.130946404332916E-4	0.0	0.1425423742320044	0.0
84-85	0.0	9.186285473450085E-4	0.0	0.1427028575324442	0.0
86-87	0.0	9.296963611684424E-4	0.0	0.14272499316009105	0.0
88	0.0	9.850354302856116E-4	0.0	0.14273052706700276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12515	0.0	53.61646	1
CTTATAC	11575	0.0	48.62404	1
TATACAC	13100	0.0	45.21176	3
TTATACA	14170	0.0	40.551174	2
GTATCAA	24515	0.0	40.02681	1
TACACAT	18670	0.0	31.768394	5
ATCAACG	30690	0.0	31.423664	3
TATCAAC	31585	0.0	30.947245	2
CAACGCA	31355	0.0	30.81091	5
TCAACGC	31690	0.0	30.6446	4
ATACACA	20775	0.0	29.380257	4
AACGCAG	33395	0.0	29.154528	6
ACACATC	19890	0.0	28.697016	6
ACATCTC	20515	0.0	27.986734	8
ACGCAGA	36165	0.0	26.723469	7
CGCAGAG	37440	0.0	25.779684	8
CACATCT	22205	0.0	25.742954	7
CATCTCC	24555	0.0	23.399258	9
GCAGAGT	43045	0.0	22.403288	9
GAGTACT	24515	0.0	20.673021	12-13
>>END_MODULE