FastQCFastQC Report
Fri 27 May 2016
SRR2139427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26953215
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA377350.056.030161
GTATCAA703300.041.1706051
ATCAACG867600.032.6428073
TCAACGC881600.032.1846774
TATCAAC900450.031.8035552
CAACGCA912900.029.8392475
AACGCAG955300.028.6802546
ACGCAGA1092550.025.0294577
CGCAGAG1105550.024.7592968
GTGGTAT182950.022.0810991
GTACATG995100.021.6174091
TGGTATC176800.021.5986522
TACATGG982600.021.4471052
GCAGAGT1270750.021.2898719
ACATGGG1014400.020.4596633
GAGTACT691200.019.78076712-13
CAGAGTA1236800.018.92642810-11
AGAGTAC1164550.018.7754110-11
CATGGGG656100.018.028694
GTACTTT765500.017.82364714-15
Produced by FastQC (version 0.11.5)