FastQCFastQC Report
Fri 27 May 2016
SRR2139426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13122849
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145700.050.526631
GTATCAA274600.039.3500981
ATCAACG315600.033.52743
TCAACGC320250.033.0675054
CAACGCA324450.032.573035
TATCAAC340950.031.4396082
AACGCAG342700.030.9383016
ACGCAGA386550.027.3728287
CGCAGAG399200.026.5054268
GCAGAGT477250.022.2068239
GAGTACT284950.020.0864512-13
CAGAGTA452950.019.56350710-11
TACATGG345150.019.306062
GTACATG357300.019.2768381
AGAGTAC423200.018.87646910-11
ACATGGG346300.018.8433783
TATACAC138350.018.7543813
CTTATAC110150.018.4301321
GTGGTAT78000.017.6273941
GTACTTT327750.017.4440914-15
Produced by FastQC (version 0.11.5)