##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13122849
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.697182905937574	32.0	18.0	33.0	18.0	33.0
2	28.813834099592246	31.0	27.0	33.0	18.0	33.0
3	30.62104387545723	32.0	29.0	33.0	27.0	33.0
4	31.88661890417241	33.0	31.0	33.0	29.0	33.0
5	32.727241775013944	33.0	33.0	34.0	32.0	34.0
6	36.87470030326494	38.0	37.0	38.0	35.0	38.0
7	37.006032531502875	38.0	38.0	38.0	35.0	38.0
8	37.22509548040978	38.0	38.0	38.0	36.0	38.0
9	37.3120132678506	38.0	38.0	38.0	37.0	38.0
10-11	37.31107315187426	38.0	38.0	38.0	37.0	38.0
12-13	37.35172991017423	38.0	38.0	38.0	37.0	38.0
14-15	37.348145703726374	38.0	38.0	38.0	37.0	38.0
16-17	37.36305877633737	38.0	38.0	38.0	37.0	38.0
18-19	37.3630889908129	38.0	38.0	38.0	37.0	38.0
20-21	37.32450940047622	38.0	38.0	38.0	37.0	38.0
22-23	37.34185217052412	38.0	38.0	38.0	37.0	38.0
24-25	37.35616706285886	38.0	38.0	38.0	37.0	38.0
26-27	37.321421153743074	38.0	38.0	38.0	37.0	38.0
28-29	37.29742020627988	38.0	38.0	38.0	37.0	38.0
30-31	37.29855065196935	38.0	38.0	38.0	37.0	38.0
32-33	37.236671152953456	38.0	38.0	38.0	37.0	38.0
34-35	37.244473905016115	38.0	38.0	38.0	37.0	38.0
36-37	37.24174768694775	38.0	38.0	38.0	37.0	38.0
38-39	37.22889093584254	38.0	38.0	38.0	37.0	38.0
40-41	37.22748376942011	38.0	38.0	38.0	37.0	38.0
42-43	37.194503909468764	38.0	38.0	38.0	37.0	38.0
44-45	37.16992792856901	38.0	38.0	38.0	36.0	38.0
46-47	37.1441522375129	38.0	38.0	38.0	36.0	38.0
48-49	37.10213296171054	38.0	38.0	38.0	36.0	38.0
50-51	37.07711060573398	38.0	38.0	38.0	36.0	38.0
52-53	37.0475573998592	38.0	38.0	38.0	36.0	38.0
54-55	37.02164242363429	38.0	38.0	38.0	36.0	38.0
56-57	36.99003966728756	38.0	38.0	38.0	36.0	38.0
58-59	36.995487660565175	38.0	38.0	38.0	36.0	38.0
60-61	37.01969517197034	38.0	38.0	38.0	36.0	38.0
62-63	37.03055630033927	38.0	38.0	38.0	36.0	38.0
64-65	37.02977639695324	38.0	38.0	38.0	36.0	38.0
66-67	37.035279268237794	38.0	38.0	38.0	36.0	38.0
68-69	37.03759638567918	38.0	38.0	38.0	36.0	38.0
70-71	37.02722279619451	38.0	38.0	38.0	36.0	38.0
72-73	37.01093368896121	38.0	38.0	38.0	36.0	38.0
74-75	37.00671009898028	38.0	38.0	38.0	36.0	38.0
76-77	37.006160296059264	38.0	38.0	38.0	36.0	38.0
78-79	36.98159955514597	38.0	38.0	38.0	36.0	38.0
80-81	36.961525030475265	38.0	38.0	38.0	36.0	38.0
82-83	36.96508343823447	38.0	38.0	38.0	36.0	38.0
84-85	36.91472858807505	38.0	38.0	38.0	35.5	38.0
86-87	36.9060586575758	38.0	38.0	38.0	35.0	38.0
88-89	36.912932309670694	38.0	38.0	38.0	35.0	38.0
90-91	36.89661120719862	38.0	38.0	38.0	35.0	38.0
92-93	36.90669189848007	38.0	38.0	38.0	35.0	38.0
94-95	36.85802838517371	38.0	38.0	38.0	35.0	38.0
96-97	36.833284013351964	38.0	38.0	38.0	35.0	38.0
98-99	36.91237034241411	38.0	38.0	38.0	35.0	38.0
100	35.365402523644406	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	7.0
19	51.0
20	133.0
21	378.0
22	1116.0
23	3085.0
24	7163.0
25	13785.0
26	24475.0
27	39038.0
28	59410.0
29	84918.0
30	115013.0
31	149770.0
32	194297.0
33	259837.0
34	380872.0
35	685611.0
36	2089693.0
37	9014194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40540312549508	18.311366685694548	13.729701530513685	23.553528658296685
2	14.403377041494863	20.029884567656815	41.60594649248024	23.960791898368083
3	18.3854816892277	25.54137443782215	30.493713674522965	25.57943019842719
4	12.29229262639538	17.76193568942232	39.65009427449786	30.295677409684433
5	13.3562441903016	37.620097582357694	35.52318034416429	13.500477883176423
6	29.88718975544381	37.94701951933702	18.79609380078219	13.369696924436973
7	26.318250428565506	32.878815635142615	23.104070092101956	17.69886384418992
8	24.303426793983533	36.497935775988886	21.214905391352136	17.98373203867544
9	25.557521846056446	16.966894917407036	21.234306666182015	36.2412765703545
10-11	24.096722441902667	26.6022530625781	29.061661838827835	20.23936265669139
12-13	24.99354370381005	24.067898670479256	29.001640573628485	21.93691705208221
14-15	22.18034361288467	25.406270391437104	26.711592886575165	25.70179310910306
16-17	21.885994420876138	28.539016184671485	27.32238632022665	22.25260307422573
18-19	21.80794734436097	27.75134804949748	28.976466924217448	21.4642376819241
20-21	22.639977736278848	26.998785462802072	28.858876081389994	21.502360719529083
22-23	22.35879165574855	26.852046212770492	28.635418316608817	22.153743814872136
24-25	22.149268968873653	26.949022900730196	28.860215910925586	22.04149221947056
26-27	22.091680675445705	27.143811930540846	28.774047387781902	21.990460006231544
28-29	21.925283984760625	27.220213477160954	28.762809990506078	22.091692547572343
30-31	22.309057939665617	26.962513155828642	28.695435452014966	22.032993452490768
32-33	22.092501935957635	27.126125538101448	28.666067867505596	22.115304658435317
34-35	22.10369342685043	27.298678929576308	28.67740217672059	21.92022546685267
36-37	22.205031718899406	27.099565585853902	28.554279481746285	22.141123213500403
38-39	22.005942242000863	27.151073996296756	28.79177038405863	22.051213377643748
40-41	22.359745429619352	27.069882930050294	28.660582868150097	21.909788772180256
42-43	22.372966755401848	27.08217407407711	28.466636582046746	22.078222588474294
44-45	22.30492061669363	27.349893395035078	28.2338692937538	22.111316694517495
46-47	22.37022714259353	27.101827293699866	28.069941323542576	22.45800424016403
48-49	22.311525811069387	27.09184007302776	28.27053387189181	22.326100244011037
50-51	22.096818121581666	27.38363223540755	28.151004554793946	22.368545088216838
52-53	22.333962627238286	27.367089738001106	28.021267971539487	22.27767966322112
54-55	22.419622396793933	27.18423232770842	27.999355412491223	22.396789863006425
56-57	22.442185410824905	27.350676671307273	27.87882177577341	22.328316142094415
58-59	22.26921960826026	27.485217592453942	28.15072696048008	22.094835838805718
60-61	22.48389274114852	27.505963477726052	27.869237379130002	22.140906401995426
62-63	22.26804174734331	27.683887806489466	27.843953458446514	22.20411698772071
64-65	22.195145097341324	27.683690522412146	27.804320170539388	22.316844209707142
66-67	22.322812358189363	27.682540816840362	27.660168766357724	22.334478058612557
68-69	22.369975789675518	27.548998970381337	27.817148193829933	22.263877046113215
70-71	22.315176699714744	27.245810836359187	27.828980018238536	22.61003244568753
72-73	22.379627969833056	27.225925396545875	27.893611105188487	22.500835528432578
74-75	22.23326147690036	27.40372998293773	27.805399758948578	22.557608781213332
76-77	22.375319294243944	27.256824722664565	27.69498179367203	22.672874189419463
78-79	22.46946669443476	27.314918364545637	27.614722803089286	22.600892137930316
80-81	22.431814347488192	27.301786739898855	27.591523183144588	22.67487572946836
82-83	22.50127193152372	27.328070752992645	27.691633857779784	22.479023457703846
84-85	22.731257767206255	27.25115802826406	27.476153391333412	22.54143081319627
86-87	22.402702285308628	27.337760929536177	27.639750028135555	22.619786757019643
88-89	22.53289537361373	27.273899045468774	27.631292306951405	22.561913273966088
90-91	22.56495990433207	27.28559234595525	27.577576608367433	22.57187114134524
92-93	22.627388761391906	27.282796340133842	27.593925923248825	22.495888975225427
94-95	22.468618602685556	27.450129495370973	27.552141387371478	22.529110514571993
96-97	22.474403667953762	27.535768043403262	27.72557564214187	22.264252646501102
98-99	23.64704794093608	29.393976795522708	27.832463123972158	19.12651213956905
100	22.52460965275504	30.314295328039975	21.711120376813955	25.449974642391027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2013.0
1	1873.0
2	2593.0
3	3853.5
4	5031.5
5	6913.5
6	8624.0
7	10735.0
8	13700.0
9	15713.5
10	15999.0
11	16315.5
12	18310.5
13	22040.5
14	28263.5
15	35233.5
16	40741.0
17	44466.0
18	44730.5
19	41609.0
20	37929.0
21	36107.5
22	38469.5
23	45999.0
24	57708.0
25	75026.0
26	99293.0
27	127311.0
28	152622.0
29	178278.5
30	201792.5
31	225090.5
32	254678.5
33	284334.0
34	313921.0
35	345103.5
36	382187.0
37	413927.0
38	428807.0
39	442324.0
40	467440.5
41	492980.5
42	509465.0
43	528680.5
44	547693.0
45	559131.5
46	557970.0
47	551108.0
48	550665.0
49	547037.0
50	540323.0
51	529720.0
52	506152.0
53	478718.0
54	452617.0
55	420969.5
56	385994.5
57	353246.5
58	320558.0
59	280388.0
60	231180.5
61	182149.5
62	141985.0
63	107703.0
64	80261.0
65	59204.5
66	42751.5
67	31748.0
68	23318.0
69	16732.5
70	11960.5
71	8489.5
72	5871.0
73	3886.0
74	2530.0
75	1605.5
76	982.5
77	599.5
78	364.0
79	231.0
80	154.5
81	98.5
82	58.5
83	34.0
84	22.0
85	16.5
86	11.5
87	7.0
88	5.5
89	3.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002720445842210026
3	0.0
4	0.0
5	0.00301001710832762
6	1.2192474362846056E-4
7	7.620296476778785E-6
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.294454672187097E-5
44-45	7.85969377689882E-6
46-47	0.0
48-49	0.0
50-51	1.6072809828523208E-5
52-53	0.0011405299533094539
54-55	0.002332870301327452
56-57	0.002673416578823884
58-59	0.0017224933508452115
60-61	0.0043828204261075416
62-63	0.0017322005384920755
64-65	0.0017204128720361132
66-67	1.236494495467608E-4
68-69	1.290894349677978E-4
70-71	1.9983111664150966E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	8.559269517702282E-5
80-81	3.001310208329127E-4
82-83	9.180306076912788E-6
84-85	4.632298133596127E-6
86-87	0.0
88-89	0.0
90-91	2.8617950456794954E-5
92-93	2.3130107868703679E-4
94-95	0.0
96-97	0.0
98-99	5.350031137181219E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	85.0
20-21	331.0
22-23	666.0
24-25	1002.0
26-27	2642.0
28-29	6755.0
30-31	21166.0
32-33	27183.0
34-35	36872.0
36-37	60064.0
38-39	70185.0
40-41	69833.0
42-43	80035.0
44-45	94547.0
46-47	96262.0
48-49	90547.0
50-51	82254.0
52-53	80164.0
54-55	86094.0
56-57	89304.0
58-59	90589.0
60-61	92292.0
62-63	91865.0
64-65	98900.0
66-67	106114.0
68-69	108303.0
70-71	116899.0
72-73	97806.0
74-75	97508.0
76-77	101575.0
78-79	103907.0
80-81	103545.0
82-83	99342.0
84-85	98935.0
86-87	103024.0
88-89	106400.0
90-91	107226.0
92-93	108588.0
94-95	133171.0
96-97	413042.0
98-99	900384.0
100-101	8847443.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.24225561343818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.60583028658465	70.29689427083609
2	8.033082755118581	12.891853596002548
3	2.1963363985172673	5.287169601087624
4	0.9282778704306943	2.9794844063759127
5	0.44645752415156637	1.791237938675637
6	0.2455649475398814	1.182281117411742
7	0.14176593117580205	0.7962932660680057
8	0.09935422171330786	0.6377925483994767
9	0.06789851763454505	0.4903497187024233
>10	0.2270950893838062	2.7975338039370405
>50	0.005615566854407541	0.30113910448509984
>100	0.0025007416753255493	0.36090189908987197
>500	1.2008819292455453E-4	0.06226404907394475
>1k	1.000610271633958E-4	0.12480467985455641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.620296476778785E-6	0.0	0.0	0.0
3	0.0	7.620296476778785E-6	0.0	0.0	0.0
4	0.0	7.620296476778785E-6	0.0	0.0	0.0
5	0.0	7.620296476778785E-6	0.0	0.0	0.0
6	0.0	7.620296476778785E-6	0.0	0.0	0.0
7	0.0	7.620296476778785E-6	0.0	0.0	0.0
8	0.0	7.620296476778785E-6	0.0	0.0	0.0
9	0.0	1.524059295355757E-5	0.0	0.0	0.0
10-11	0.0	1.524059295355757E-5	0.0	7.620296476778785E-6	0.0
12-13	0.0	1.524059295355757E-5	0.0	1.524059295355757E-5	0.0
14-15	0.0	1.524059295355757E-5	0.0	1.524059295355757E-5	0.0
16-17	0.0	1.524059295355757E-5	0.0	1.524059295355757E-5	0.0
18-19	0.0	2.2860889430336356E-5	0.0	1.524059295355757E-5	0.0
20-21	0.0	3.048118590711514E-5	0.0	1.524059295355757E-5	0.0
22-23	0.0	4.1911630622283315E-5	0.0	1.524059295355757E-5	3.8101482383893925E-6
24-25	0.0	4.572177886067271E-5	0.0	4.1911630622283315E-5	7.620296476778785E-6
26-27	0.0	4.572177886067271E-5	0.0	8.001311300617725E-5	7.620296476778785E-6
28-29	0.0	4.572177886067271E-5	0.0	3.01001710832762E-4	7.620296476778785E-6
30-31	0.0	6.096237181423028E-5	0.0	0.0011049429891329238	7.620296476778785E-6
32-33	0.0	6.096237181423028E-5	0.0	0.002990966367135673	7.620296476778785E-6
34-35	0.0	8.001311300617725E-5	0.0	0.005520904797426229	7.620296476778785E-6
36-37	0.0	9.90638541981242E-5	0.0	0.00927390081223978	7.620296476778785E-6
38-39	0.0	1.0668415067490299E-4	0.0	0.01868877710929997	7.620296476778785E-6
40-41	0.0	1.2192474362846056E-4	0.0	0.02879710038574703	7.620296476778785E-6
42-43	0.0	1.2573489186684994E-4	0.0	0.03549153084059719	7.620296476778785E-6
44-45	0.0	1.2954504010523934E-4	0.0	0.043081346131468856	7.620296476778785E-6
46-47	0.0	1.2954504010523934E-4	0.0	0.05153987522069331	7.620296476778785E-6
48-49	0.0	1.2954504010523934E-4	0.0	0.05977360556385279	7.620296476778785E-6
50-51	0.0	1.2954504010523934E-4	0.0	0.06804162724115777	7.620296476778785E-6
52-53	0.0	1.2954504010523934E-4	0.0	0.08770580229948542	7.620296476778785E-6
54-55	0.0	1.2954504010523934E-4	0.0	0.11084102240298582	7.620296476778785E-6
56-57	0.0	1.2954504010523934E-4	0.0	0.13871606691504262	7.620296476778785E-6
58-59	0.0	1.3335518834362874E-4	0.0	0.16183985657382782	7.620296476778785E-6
60-61	0.0	1.447856330587969E-4	0.0	0.17099183264243917	7.620296476778785E-6
62-63	3.8101482383893925E-6	1.600262260123545E-4	0.0	0.18219747861154234	7.620296476778785E-6
64-65	7.620296476778785E-6	1.7526681896591205E-4	0.0	0.18784411830083542	7.620296476778785E-6
66-67	7.620296476778785E-6	1.7526681896591205E-4	0.0	0.1932469085028716	7.620296476778785E-6
68-69	7.620296476778785E-6	1.7526681896591205E-4	0.0	0.19871066107672197	7.620296476778785E-6
70-71	7.620296476778785E-6	1.7907696720430145E-4	0.0	0.20376291764082632	7.620296476778785E-6
72-73	7.620296476778785E-6	1.8288711544269085E-4	0.0	0.20751591365563987	7.620296476778785E-6
74-75	7.620296476778785E-6	1.8669726368108022E-4	0.0	0.21047258868863006	7.620296476778785E-6
76-77	7.620296476778785E-6	1.9050741191946961E-4	0.0	0.21340259268395148	7.620296476778785E-6
78-79	7.620296476778785E-6	1.9050741191946961E-4	0.0	0.21527337546900066	7.620296476778785E-6
80-81	7.620296476778785E-6	1.9050741191946961E-4	0.0	0.2159477717071956	7.620296476778785E-6
82-83	7.620296476778785E-6	1.9050741191946961E-4	0.0	0.21636307786518005	7.620296476778785E-6
84-85	7.620296476778785E-6	1.9050741191946961E-4	0.0	0.216587876611245	7.620296476778785E-6
86-87	7.620296476778785E-6	1.9050741191946961E-4	0.0	0.21667932016896635	7.620296476778785E-6
88	7.620296476778785E-6	2.057480048730272E-4	0.0	0.21668313031720474	7.620296476778785E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14570	0.0	50.52663	1
GTATCAA	27460	0.0	39.350098	1
ATCAACG	31560	0.0	33.5274	3
TCAACGC	32025	0.0	33.067505	4
CAACGCA	32445	0.0	32.57303	5
TATCAAC	34095	0.0	31.439608	2
AACGCAG	34270	0.0	30.938301	6
ACGCAGA	38655	0.0	27.372828	7
CGCAGAG	39920	0.0	26.505426	8
GCAGAGT	47725	0.0	22.206823	9
GAGTACT	28495	0.0	20.08645	12-13
CAGAGTA	45295	0.0	19.563507	10-11
TACATGG	34515	0.0	19.30606	2
GTACATG	35730	0.0	19.276838	1
AGAGTAC	42320	0.0	18.876469	10-11
ACATGGG	34630	0.0	18.843378	3
TATACAC	13835	0.0	18.754381	3
CTTATAC	11015	0.0	18.430132	1
GTGGTAT	7800	0.0	17.627394	1
GTACTTT	32775	0.0	17.44409	14-15
>>END_MODULE