FastQCFastQC Report
Fri 27 May 2016
SRR2139425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12123274
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165450.050.4597741
GTATCAA309250.039.3178061
CTTATAC106650.037.666481
TATACAC133650.033.9223983
ATCAACG367500.032.5580443
TCAACGC374250.032.0398524
CAACGCA375450.031.8686435
TATCAAC383200.031.4381142
AACGCAG402350.029.865796
TTATACA153800.028.2467922
ACGCAGA439300.027.275347
CGCAGAG465950.025.6783668
TACACAT182700.024.8622865
ACACATC187400.022.6989366
GCAGAGT531900.022.5107089
ACATCTC190600.022.159728
ATACACA220750.021.7470954
TACATGG390350.020.9682522
GTACATG405200.020.7948461
GAGTACT302650.020.32870712-13
Produced by FastQC (version 0.11.5)