FastQCFastQC Report
Fri 27 May 2016
SRR2139424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19742527
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA220450.055.959061
GTATCAA419450.042.647261
ATCAACG517050.033.657683
TCAACGC521500.033.4401554
TATCAAC536150.032.7331922
CAACGCA540350.030.7585895
AACGCAG565750.029.5186676
ACGCAGA644600.025.8346827
CGCAGAG652650.025.5211778
GCAGAGT765300.021.5901059
GTGGTAT108250.021.020071
TGGTATC101350.021.0131472
GTACATG512450.020.5684131
TACATGG503300.020.456622
GAGTACT474450.019.58807812-13
AGAGTAC695800.019.29355810-11
ACATGGG526650.019.1401843
CAGAGTA747200.018.75049410-11
GTACTTT533250.017.50570714-15
AGTACTT518150.017.3519912-13
Produced by FastQC (version 0.11.5)