##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139424_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19742527
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83498855035123	33.0	31.0	34.0	30.0	34.0
2	31.439523167424312	33.0	31.0	34.0	28.0	34.0
3	31.762404934282223	33.0	31.0	34.0	30.0	34.0
4	32.86444045384869	37.0	35.0	37.0	26.0	37.0
5	34.38652123915039	37.0	35.0	37.0	27.0	37.0
6	35.137970635673945	37.0	35.0	37.0	32.0	37.0
7	35.279934275890824	37.0	35.0	37.0	32.0	37.0
8	35.42511721017274	37.0	35.0	37.0	32.0	37.0
9	37.190714744875365	39.0	37.0	39.0	33.0	39.0
10-11	37.053803775980654	39.0	37.0	39.0	33.0	39.0
12-13	36.952887616666295	39.0	37.0	39.0	32.5	39.0
14-15	38.30441943931494	40.0	38.0	41.0	33.0	41.0
16-17	38.30544463735569	40.0	38.0	41.0	33.0	41.0
18-19	38.23378775168953	40.0	38.0	41.0	33.0	41.0
20-21	38.298148609447466	40.0	38.0	41.0	32.5	41.0
22-23	38.34664159927101	40.0	38.0	41.0	33.0	41.0
24-25	38.22600883507596	40.0	38.0	41.0	32.0	41.0
26-27	38.13291362741276	40.0	38.0	41.0	32.0	41.0
28-29	38.14539184422318	40.0	38.0	41.0	32.0	41.0
30-31	38.098411917771756	40.0	37.5	41.0	32.0	41.0
32-33	38.00291175959347	40.0	37.0	41.0	32.0	41.0
34-35	37.90027313866047	40.0	37.0	41.0	31.0	41.0
36-37	37.8592893323122	40.0	37.0	41.0	31.0	41.0
38-39	37.79866931889808	40.0	37.0	41.0	31.0	41.0
40-41	37.708351108019535	40.0	37.0	41.0	31.0	41.0
42-43	37.68147468210757	40.0	37.0	41.0	31.0	41.0
44-45	37.618770365567144	40.0	37.0	41.0	31.0	41.0
46-47	37.48179587934692	40.0	36.0	41.0	30.0	41.0
48-49	37.65338183717719	40.0	37.0	41.0	31.0	41.0
50-51	37.61347704072172	40.0	36.5	41.0	31.0	41.0
52-53	37.551928038353836	40.0	36.0	41.0	31.0	41.0
54-55	37.42165566185268	39.5	36.0	41.0	30.0	41.0
56-57	37.28614909300328	39.0	35.5	41.0	30.0	41.0
58-59	37.18004267384678	39.0	35.0	41.0	30.5	41.0
60-61	36.96689454294621	39.0	35.0	41.0	30.5	41.0
62-63	36.78460061999648	39.0	35.0	41.0	30.0	41.0
64-65	36.521239249232565	38.0	35.0	40.0	30.0	41.0
66-67	36.30521973085914	37.5	35.0	40.0	30.0	41.0
68-69	36.05038745270309	37.0	34.0	40.0	30.0	41.0
70-71	35.80301338547852	37.0	34.0	39.0	30.0	41.0
72-73	35.49400684625034	36.0	34.0	39.0	30.0	41.0
74-75	35.31274430993666	36.0	34.0	39.0	30.0	40.5
76-77	35.10894005955193	35.5	34.0	38.5	30.0	40.0
78-79	34.84910415377402	35.0	34.0	37.0	30.0	39.0
80-81	34.59318157396117	35.0	34.0	37.0	30.0	39.0
82-83	34.34236994736949	35.0	34.0	36.0	30.0	39.0
84-85	34.12773886790325	35.0	34.0	36.0	30.0	37.0
86-87	33.93997258723077	35.0	34.0	36.0	29.5	37.0
88-89	33.80075959800827	35.0	34.0	35.0	29.0	36.5
90-91	33.663598588250565	35.0	34.0	35.0	29.0	36.0
92-93	33.62076353525107	35.0	34.0	35.0	29.0	36.0
94-95	33.59696300953926	35.0	34.0	35.0	29.5	36.0
96-97	33.60537900390003	35.0	34.0	35.0	30.0	36.0
98-99	33.63503616242233	35.0	34.0	35.0	30.0	35.0
100-101	33.24633802791493	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	10.0
17	140.0
18	848.0
19	3246.0
20	9245.0
21	20306.0
22	37203.0
23	60396.0
24	95044.0
25	147792.0
26	225681.0
27	323305.0
28	365670.0
29	294278.0
30	349722.0
31	432856.0
32	544219.0
33	715622.0
34	984218.0
35	1399489.0
36	2136139.0
37	3713469.0
38	5015661.0
39	2731529.0
40	136438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46722590274284	20.389436468796525	14.69169068377113	24.4516469446895
2	14.134381676430516	20.98891999547178	41.76465343472504	23.11204489337267
3	17.36875436458837	26.258407135591	31.027336342056277	25.34550215776435
4	11.97460182003188	18.380460092859042	39.143122206385506	30.501815880723576
5	12.960857611506245	37.868317214182895	35.77152167316138	13.399303501149475
6	29.37565633140543	38.551043289727524	18.87011114997854	13.203189228888501
7	25.85720042070838	33.1299490651834	23.371846251444037	17.64100426266418
8	24.08501066941351	36.99737661287251	21.343557951569856	17.574054766144123
9	25.350256378800072	16.92794016066989	21.3558366337905	36.36596682673954
10-11	23.75116698913792	26.974242832248756	29.29802229681142	19.9765678818019
12-13	24.670158681235307	24.569271542062882	29.271255998772006	21.4893137779298
14-15	21.857585954771192	25.96695302850373	26.930741564963512	25.244719451761565
16-17	21.419144158432843	29.091722057961512	27.558585229654962	21.930548553950686
18-19	21.464667213206887	28.157929353771166	29.35567764471302	21.021725788308927
20-21	22.21531062653784	27.339700731621868	29.259254165201032	21.185734476639258
22-23	21.930050067894832	27.29367980519578	29.114579273085845	21.66169085382354
24-25	21.775386570547166	27.384309126853918	29.18879416880972	21.65151013378919
26-27	21.758231622241087	27.57861503609101	29.104601407070458	21.558551934597443
28-29	21.63139587440262	27.54791267390156	29.18344025470349	21.63725119699233
30-31	21.930888311534197	27.384733694195397	29.13631941370155	21.548058580568856
32-33	21.638665130812818	27.46724524899944	29.16460608311755	21.72948353707019
34-35	21.750169378237207	27.519964399620996	29.049770923639574	21.68009529850223
36-37	21.806733485889097	27.47447275394581	29.0494665360998	21.66932722406529
38-39	21.68921217668759	27.536587534572725	29.082680172782226	21.691520115957463
40-41	21.926180992824147	27.38859370447237	28.991902211393445	21.693323091310038
42-43	21.882033366370244	27.47863885411742	28.818293662307877	21.821034117204455
44-45	21.888180162545186	27.648202401045324	28.604023774676357	21.859593661733136
46-47	22.04861642364271	27.53529269604415	28.284039904970793	22.132050975342352
48-49	21.93743242529422	27.642926060818407	28.338087160254272	22.081554353633102
50-51	21.87912062497079	27.805607341203963	28.221163920701997	22.094108113123255
52-53	22.033748284191255	27.774313005871026	28.167910491121777	22.02402821881594
54-55	21.998761586740986	27.694663990083722	28.14717081972144	22.15940360345385
56-57	22.045244196178956	27.82013790666682	28.040823752300092	22.093794144854133
58-59	21.90512084340092	27.96038418145744	28.160134367947553	21.974360607194093
60-61	22.07227894734909	27.953796240984254	27.990776159710855	21.9831486519558
62-63	21.97159515483963	28.085904027844897	27.934103130701516	22.008397686613954
64-65	21.936414432196337	28.00422443408196	27.94251341036252	22.116847723359186
66-67	22.047105331037177	27.99346064223771	27.82116521904111	22.138268807684003
68-69	22.010967390898152	27.96157069883843	27.887177809354004	22.14028410090942
70-71	22.03462061587385	27.684936775452286	27.948114757601168	22.3323278510727
72-73	22.09180731351605	27.70425786950441	27.87293610832598	22.330998708653556
74-75	21.953609904858542	27.846683526792365	27.886819185982286	22.31288738236681
76-77	22.1112915825087	27.747105950459495	27.815550157865264	22.326052309166545
78-79	22.080457228995503	27.78745433303847	27.790685487789542	22.341402950176487
80-81	22.105705150604415	27.79420058724082	27.752522242625183	22.347572019529583
82-83	22.130589863209735	27.75380375858586	27.86972627194147	22.245880106262934
84-85	22.229591728302633	27.738071103845325	27.802258664000284	22.230078503851765
86-87	22.054437376160184	27.765131471374737	27.858911767470186	22.321519384994897
88-89	22.089115412178252	27.796737424844824	27.836870786180302	22.277276376796625
90-91	22.124696369096423	27.81363812622766	27.879920255601675	22.181745249074243
92-93	22.139853547690173	27.807214258052987	27.85734594856671	22.195586245690134
94-95	21.986074508571203	27.870052144285225	27.89736367196206	22.246509675181514
96-97	22.036557412153943	27.875305540502186	27.912051967714905	22.17608507962896
98-99	22.302830957844773	28.467254322306744	27.90317488464959	21.32673983519889
100-101	22.942131683113438	31.05914247467802	25.113635795935874	20.885090046272673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6467.0
1	5134.0
2	5360.0
3	7104.0
4	8888.0
5	13858.0
6	18427.0
7	22476.0
8	25704.5
9	25554.5
10	22482.5
11	19228.0
12	20422.0
13	27176.5
14	44631.5
15	69175.5
16	89164.5
17	100806.0
18	103396.5
19	97995.0
20	89353.0
21	81893.0
22	80016.5
23	88688.5
24	107497.5
25	135282.0
26	172303.0
27	213563.5
28	257654.0
29	304748.0
30	349335.0
31	393614.0
32	440482.0
33	489255.5
34	536767.5
35	579280.5
36	625642.0
37	667553.0
38	695207.0
39	718864.5
40	747934.5
41	772551.0
42	786577.5
43	801892.5
44	816549.0
45	826688.5
46	835011.5
47	838675.5
48	830388.0
49	809367.0
50	781849.5
51	747999.0
52	709114.0
53	669147.5
54	626792.5
55	580785.0
56	530360.0
57	476703.0
58	423171.0
59	367908.5
60	307506.5
61	249151.0
62	197085.0
63	152356.5
64	115248.5
65	86294.5
66	64835.5
67	49163.5
68	37165.5
69	27298.0
70	20152.5
71	14515.5
72	10407.0
73	7302.0
74	4912.5
75	3352.5
76	2265.0
77	1522.5
78	1085.5
79	781.5
80	557.5
81	416.0
82	324.0
83	229.5
84	155.0
85	109.0
86	81.0
87	64.0
88	48.0
89	36.0
90	27.0
91	21.0
92	16.5
93	10.5
94	6.5
95	5.5
96	4.5
97	3.5
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.83284034510881
3	0.006387226923888719
4	8.97368913310844
5	3.4443412436513317E-4
6	0.0734353813977309
7	0.027048209178085463
8	5.622380559489674E-4
9	3.039124626751175E-4
10-11	0.2981406584881459
12-13	0.31213835999819073
14-15	1.1143456964754309E-4
16-17	1.494236274819328E-4
18-19	0.5012478898977826
20-21	2.558280154878787E-4
22-23	1.697462951604318E-4
24-25	0.179057434548163
26-27	0.16471723676243702
28-29	1.2431128692955357E-4
30-31	1.397001445617096E-4
32-33	1.3745689771694784E-4
34-35	3.599027313094854E-4
36-37	2.690314514164813E-4
38-39	0.0012878959520193848
40-41	0.00160390725280641
42-43	0.0010873978413197098
44-45	7.857415501577068E-4
46-47	0.001325813531115014
48-49	0.002305426977780204
50-51	3.423313004155282E-4
52-53	0.0014634185941640766
54-55	5.442403102662046E-4
56-57	0.01035128826537715
58-59	1.4731842934093904E-4
60-61	0.002427887194529527
62-63	0.001425579247639238
64-65	0.0014133403511154296
66-67	0.004406987486318654
68-69	0.0018363958975731824
70-71	0.0023812710906793333
72-73	0.00330859165909701
74-75	6.317693712071651E-4
76-77	2.736301444101329E-4
78-79	7.87030548191064E-4
80-81	4.602228318101668E-4
82-83	0.001475275607591787
84-85	2.1634422045323348E-4
86-87	3.7015012806389757E-4
88-89	1.2704538178309952E-4
90-91	2.3091317087492176E-4
92-93	0.0
94-95	0.0
96-97	3.785806371766805E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1671.0
20-21	4274.0
22-23	5614.0
24-25	7799.0
26-27	10748.0
28-29	18052.0
30-31	40075.0
32-33	50520.0
34-35	66991.0
36-37	96454.0
38-39	114730.0
40-41	118790.0
42-43	139285.0
44-45	168172.0
46-47	165695.0
48-49	150213.0
50-51	135199.0
52-53	131424.0
54-55	141540.0
56-57	149202.0
58-59	152844.0
60-61	156653.0
62-63	162958.0
64-65	172290.0
66-67	181459.0
68-69	187495.0
70-71	180560.0
72-73	169321.0
74-75	172909.0
76-77	178648.0
78-79	185543.0
80-81	184952.0
82-83	180170.0
84-85	180145.0
86-87	184018.0
88-89	190435.0
90-91	195396.0
92-93	198913.0
94-95	217958.0
96-97	332610.0
98-99	1618401.0
100-101	1.2642401E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.5187241764838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.48808454305599	75.63418734576413
2	7.222079092527892	12.208018216042307
3	1.833022939960912	4.647742808151713
4	0.6719246657624208	2.2716086197180045
5	0.3145499035970763	1.3292678270930427
6	0.1629450580696309	0.8263145051344962
7	0.09139067555417936	0.5406956309626345
8	0.05693646895210349	0.3849758171956615
9	0.036870394961338815	0.2804614867813872
>10	0.11849648817987576	1.5555535636023783
>50	0.0028179904470320757	0.15681363676897855
>100	8.297424187158951E-4	0.1099773829957845
>500	1.0426939348462455E-5	0.005992646953423842
>1k	4.1609573510402425E-5	0.048390512836163906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.065207711251959E-6	1.0130415422503918E-5
2	0.0	5.065207711251959E-6	0.0	5.065207711251959E-6	1.0130415422503918E-5
3	0.0	1.0130415422503918E-5	0.0	5.065207711251959E-6	1.0130415422503918E-5
4	0.0	4.5586869401267626E-5	0.0	1.0130415422503918E-5	1.0130415422503918E-5
5	0.0	4.5586869401267626E-5	0.0	1.0130415422503918E-5	1.0130415422503918E-5
6	5.5717284823771544E-5	4.5586869401267626E-5	0.0	1.0130415422503918E-5	1.0130415422503918E-5
7	5.5717284823771544E-5	4.5586869401267626E-5	0.0	1.5195623133755876E-5	1.0130415422503918E-5
8	6.0782492535023504E-5	5.5717284823771544E-5	0.0	2.0260830845007837E-5	1.5195623133755876E-5
9	6.0782492535023504E-5	1.1143456964754309E-4	0.0	4.5586869401267626E-5	1.5195623133755876E-5
10-11	6.584770024627547E-5	1.1143456964754309E-4	0.0	4.8119473256893606E-5	1.5195623133755876E-5
12-13	7.597811566877939E-5	1.1143456964754309E-4	0.0	5.0652077112519585E-5	2.0260830845007837E-5
14-15	9.87715503694132E-5	1.3422800434817692E-4	0.0	6.0782492535023504E-5	2.5326038556259793E-5
16-17	1.4435841977068084E-4	1.367606082038029E-4	0.0	6.0782492535023504E-5	3.0391246267511752E-5
18-19	1.4689102362630682E-4	1.494236274819328E-4	0.0	7.851071952440537E-5	4.052166169001567E-5
20-21	1.4689102362630682E-4	1.6715185447131463E-4	0.0	9.877155036941319E-5	5.0652077112519585E-5
22-23	1.5448883519318476E-4	2.076735161613303E-4	0.0	1.2916279663692496E-4	5.0652077112519585E-5
24-25	1.7474966603819257E-4	2.4059736628446803E-4	0.0	2.0514091230570432E-4	5.0652077112519585E-5
26-27	1.823474776050705E-4	2.5326038556259796E-4	0.0	4.432056747345464E-4	5.3184680968145565E-5
28-29	1.823474776050705E-4	2.5326038556259796E-4	0.0	0.0017626922835156816	5.5717284823771544E-5
30-31	1.9247789302757442E-4	2.735212164076058E-4	0.0	0.006442944208712492	5.5717284823771544E-5
32-33	1.9754310073882638E-4	2.8111902797448376E-4	0.0	0.017452173169118623	5.5717284823771544E-5
34-35	2.1780393158383422E-4	3.191080858088734E-4	0.0	0.033344262363171645	5.5717284823771544E-5
36-37	2.329995547175901E-4	3.5962974749888905E-4	0.0	0.057034238828697056	5.5717284823771544E-5
38-39	2.329995547175901E-4	3.8495578605514884E-4	0.0	0.09319475667932478	5.5717284823771544E-5
40-41	2.4312997014009401E-4	4.3560786316766844E-4	0.0	0.13458256888796455	5.5717284823771544E-5
42-43	2.4312997014009401E-4	4.508034863014243E-4	0.0	0.17573231633417546	5.5717284823771544E-5
44-45	2.5326038556259796E-4	5.115859788364478E-4	0.0	0.22280582419869555	5.5717284823771544E-5
46-47	2.6592340484072783E-4	5.521076405264635E-4	0.0	0.2748457682240982	5.5717284823771544E-5
48-49	2.6845600869635384E-4	5.571728482377154E-4	0.0	0.3255738234522865	5.5717284823771544E-5
50-51	2.6845600869635384E-4	6.635422101740066E-4	0.0	0.37638545460771056	5.5717284823771544E-5
52-53	2.7098861255197984E-4	6.736726255965105E-4	0.0	0.4255027737837208	5.5717284823771544E-5
54-55	2.735212164076058E-4	6.787378333077624E-4	0.0	0.4816050143935475	5.5717284823771544E-5
56-57	2.735212164076058E-4	6.939334564415184E-4	0.0	0.5427446040722143	7.344551181315341E-5
58-59	2.7858642411885776E-4	6.964660602971443E-4	0.0	0.6039221828087153	8.104332338003135E-5
60-61	2.836516318301097E-4	7.040638718640222E-4	0.0	0.6652947720420997	8.104332338003135E-5
62-63	2.988472549638656E-4	7.091290795752742E-4	0.0	0.6883845213938418	8.864113494690927E-5
64-65	2.988472549638656E-4	8.256288569340693E-4	0.0	0.6952808016927114	9.117373880253525E-5
66-67	3.013798588194916E-4	8.458896877790771E-4	0.0	0.7008905192329229	9.117373880253525E-5
68-69	3.0391246267511755E-4	8.737483301909628E-4	0.0	0.7064521172998777	9.117373880253525E-5
70-71	3.115102742419955E-4	8.889439533247188E-4	0.0	0.7112919232679789	9.370634265816123E-5
72-73	3.241732935201254E-4	8.940091610359707E-4	0.0	0.7154555240066279	9.623894651378721E-5
74-75	3.241732935201254E-4	9.041395764584747E-4	0.0	0.7187276481880966	9.623894651378721E-5
76-77	3.292385012313773E-4	9.345308227259863E-4	0.0	0.721819957495816	9.623894651378721E-5
78-79	3.393689166538812E-4	9.471938420041163E-4	0.0	0.7247527127606309	9.623894651378721E-5
80-81	3.393689166538812E-4	9.471938420041163E-4	0.0	0.7260848623886902	1.0383675808066515E-4
82-83	3.393689166538812E-4	9.471938420041163E-4	0.0	0.7265913831598154	1.1143456964754309E-4
84-85	3.393689166538812E-4	9.623894651378721E-4	0.0	0.7269560781150255	1.1143456964754309E-4
86-87	3.4190152050950716E-4	9.8265029598288E-4	0.0	0.7270523170615393	1.1143456964754309E-4
88-89	3.4443412436513317E-4	0.0010636936193629113	0.0	0.72707511049624	1.24097588925673E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22045	0.0	55.95906	1
GTATCAA	41945	0.0	42.64726	1
ATCAACG	51705	0.0	33.65768	3
TCAACGC	52150	0.0	33.440155	4
TATCAAC	53615	0.0	32.733192	2
CAACGCA	54035	0.0	30.758589	5
AACGCAG	56575	0.0	29.518667	6
ACGCAGA	64460	0.0	25.834682	7
CGCAGAG	65265	0.0	25.521177	8
GCAGAGT	76530	0.0	21.590105	9
GTGGTAT	10825	0.0	21.02007	1
TGGTATC	10135	0.0	21.013147	2
GTACATG	51245	0.0	20.568413	1
TACATGG	50330	0.0	20.45662	2
GAGTACT	47445	0.0	19.588078	12-13
AGAGTAC	69580	0.0	19.293558	10-11
ACATGGG	52665	0.0	19.140184	3
CAGAGTA	74720	0.0	18.750494	10-11
GTACTTT	53325	0.0	17.505707	14-15
AGTACTT	51815	0.0	17.35199	12-13
>>END_MODULE