FastQCFastQC Report
Fri 27 May 2016
SRR2139423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11357612
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130600.051.8625561
CTTATAC140050.048.8281141
GTATCAA250400.039.4995231
TATACAC191000.039.257263
TTATACA202800.036.0106162
ATCAACG284250.033.9852563
TCAACGC290050.033.3505744
CAACGCA290700.033.2311975
AACGCAG302200.032.12386
TATCAAC306900.031.8315752
ACGCAGA339150.028.521417
CGCAGAG344450.028.0699528
TACACAT275300.027.3466345
ACATCTC268350.026.6626478
ACACATC271800.026.2923836
CACATCT294100.024.3872417
CATCTCC297600.023.8815989
GCAGAGT410200.023.613029
ATACACA336600.023.3982944
GAGTACT254500.020.76110312-13
Produced by FastQC (version 0.11.5)