##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11357612
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.46859436649183	32.0	18.0	33.0	18.0	33.0
2	28.711860820742952	31.0	27.0	33.0	18.0	33.0
3	30.575883205025846	32.0	29.0	33.0	27.0	33.0
4	31.89709236413429	33.0	31.0	33.0	29.0	33.0
5	32.73045020379284	33.0	33.0	34.0	32.0	34.0
6	36.856549246443706	38.0	37.0	38.0	35.0	38.0
7	36.98131182857805	38.0	38.0	38.0	35.0	38.0
8	37.185733585545975	38.0	38.0	38.0	36.0	38.0
9	37.278049558305035	38.0	38.0	38.0	37.0	38.0
10-11	37.273496268405715	38.0	38.0	38.0	36.5	38.0
12-13	37.31580564646864	38.0	38.0	38.0	37.0	38.0
14-15	37.31094489757178	38.0	38.0	38.0	37.0	38.0
16-17	37.32226409037392	38.0	38.0	38.0	37.0	38.0
18-19	37.32455290777674	38.0	38.0	38.0	37.0	38.0
20-21	37.289746202442736	38.0	38.0	38.0	37.0	38.0
22-23	37.30412949428862	38.0	38.0	38.0	37.0	38.0
24-25	37.316419961926115	38.0	38.0	38.0	37.0	38.0
26-27	37.28100382629849	38.0	38.0	38.0	37.0	38.0
28-29	37.2569878594246	38.0	38.0	38.0	37.0	38.0
30-31	37.26160339655504	38.0	38.0	38.0	37.0	38.0
32-33	37.18737145698443	38.0	38.0	38.0	36.5	38.0
34-35	37.19262609192537	38.0	38.0	38.0	36.0	38.0
36-37	37.18992044415758	38.0	38.0	38.0	36.0	38.0
38-39	37.154094912515326	38.0	38.0	38.0	36.0	38.0
40-41	37.18551626151673	38.0	38.0	38.0	36.0	38.0
42-43	37.157161581322875	38.0	38.0	38.0	36.0	38.0
44-45	37.13200840304944	38.0	38.0	38.0	36.0	38.0
46-47	37.103710601688505	38.0	38.0	38.0	36.0	38.0
48-49	37.06193178351779	38.0	38.0	38.0	36.0	38.0
50-51	37.03537752485377	38.0	38.0	38.0	36.0	38.0
52-53	37.00413414432803	38.0	38.0	38.0	36.0	38.0
54-55	36.97496268382221	38.0	38.0	38.0	36.0	38.0
56-57	36.9431440722022	38.0	38.0	38.0	36.0	38.0
58-59	36.94133446755055	38.0	38.0	38.0	36.0	38.0
60-61	36.96196451120436	38.0	38.0	38.0	36.0	38.0
62-63	36.969931421011694	38.0	38.0	38.0	36.0	38.0
64-65	36.96465194912194	38.0	38.0	38.0	36.0	38.0
66-67	36.962873743665845	38.0	38.0	38.0	36.0	38.0
68-69	36.958843883959815	38.0	38.0	38.0	36.0	38.0
70-71	36.94393014047751	38.0	38.0	38.0	36.0	38.0
72-73	36.92046838965196	38.0	38.0	38.0	36.0	38.0
74-75	36.920480005796136	38.0	38.0	38.0	36.0	38.0
76-77	36.921914945241454	38.0	38.0	38.0	36.0	38.0
78-79	36.898855047640254	38.0	38.0	38.0	35.0	38.0
80-81	36.876567999314304	38.0	38.0	38.0	35.0	38.0
82-83	36.8782666332137	38.0	38.0	38.0	35.0	38.0
84-85	36.83127634154345	38.0	38.0	38.0	35.0	38.0
86-87	36.81827423938418	38.0	38.0	38.0	35.0	38.0
88-89	36.82711209263365	38.0	38.0	38.0	35.0	38.0
90-91	36.80585236879665	38.0	38.0	38.0	35.0	38.0
92-93	36.815767443344825	38.0	38.0	38.0	35.0	38.0
94-95	36.76712012829391	38.0	38.0	38.0	35.0	38.0
96-97	36.74403011689422	38.0	38.0	38.0	35.0	38.0
98-99	36.82316178050985	38.0	38.0	38.0	35.0	38.0
100	35.20024092490921	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	16.0
19	85.0
20	165.0
21	425.0
22	1159.0
23	3064.0
24	6732.0
25	13062.0
26	22847.0
27	36553.0
28	55793.0
29	79762.0
30	107704.0
31	140246.0
32	181358.0
33	242395.0
34	354093.0
35	628706.0
36	1866662.0
37	7616782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42875069160665	19.031104425824726	14.047266273931527	23.492878608637096
2	13.65744576578582	20.19754846964696	42.86383206451807	23.28117370004915
3	17.824697656514417	26.40571803298088	31.111672066275904	24.657912244228804
4	11.68123193502296	18.695312007488898	40.11483223762178	29.508623819866358
5	12.67181846165333	38.089354757136704	36.256275239193414	12.982551542016543
6	28.42750479216602	38.9453704079533	19.330881465351965	13.296243334528715
7	25.35268857573229	33.20386362907978	23.47939866232444	17.964049132863494
8	23.406372748074155	37.350096129362406	21.855967610092687	17.387563512470756
9	24.771941496152536	17.263761079353653	21.57192022407527	36.392377200418544
10-11	23.335358700402868	27.091606052399044	29.644554682797757	19.928480564400335
12-13	24.438543947442472	24.44478645687139	29.491150956732803	21.625518638953327
14-15	21.583590811166996	25.944947758384423	26.921821242000522	25.549640188448063
16-17	21.333921250347345	29.336294460490464	27.502326193217375	21.827458095944817
18-19	21.25211268002464	28.18586336634849	29.339961604604913	21.22206234902196
20-21	21.917204549880942	27.38419442193359	29.195282159323316	21.503318868862152
22-23	21.562215322606352	27.731762728584997	29.05211111868343	21.653910830125223
24-25	21.52274077718906	27.730996020258324	29.336563255843608	21.409699946709008
26-27	21.531537930008394	27.773807343717383	29.356931023895417	21.33772370237881
28-29	21.047836488500607	27.59795414635766	29.709493063738012	21.644716301403715
30-31	21.567818539501303	27.402617597100758	29.130228510181528	21.899335353216408
32-33	21.727863914344	27.4788788680176	29.540816590039054	21.252440627599352
34-35	21.592353676265013	27.93394637405628	29.36780457417295	21.105895375505764
36-37	21.37583412693723	27.70917917136219	29.183703199771884	21.73128350192869
38-39	21.26777175693812	27.57234505343056	29.54851692874384	21.61136626088748
40-41	21.53051039744604	27.38284646515402	29.608220828420457	21.478422308979482
42-43	21.759850711483463	27.336830550396552	29.456338315542364	21.446980422577617
44-45	21.4230391814941	27.848608854379712	29.243921038167304	21.48443092595888
46-47	21.79600957043944	27.381282993402756	28.785244876797133	22.03746255936067
48-49	21.751928933136817	27.36987228380866	28.881371328948045	21.996827454106473
50-51	21.39629789691489	27.5861336665219	29.109235027439446	21.908333409123763
52-53	21.516339426194854	27.67310050122107	29.350070264744744	21.46048980783933
54-55	21.55251819660081	27.710329168639735	28.890303894675	21.84684874008445
56-57	21.94027960131272	28.09159023481534	28.326979726217296	21.64115043765465
58-59	21.356000062788375	28.47692044958746	29.043713191024363	21.1233662965998
60-61	21.737559060866662	28.9847018327513	28.124656443013585	21.153082663368455
62-63	21.298393099245782	29.38793874643301	28.066387422351468	21.247280731969738
64-65	21.278632931061466	29.32097661653314	28.08450860704263	21.315881845362757
66-67	21.365997687308415	29.103190408928576	28.10569424210896	21.42511766165405
68-69	21.451067223981735	28.8264472227104	28.301691290774528	21.42079426253334
70-71	21.546837070821493	28.360934286495265	28.401910979067917	21.69031766361532
72-73	21.7183905761993	27.981668219801847	28.60024264872434	21.699698555274512
74-75	21.694497518285296	28.07926408377977	28.488067530930174	21.73817086700476
76-77	21.68533875746002	28.08937443688518	28.382673173152433	21.84261363250237
78-79	21.670454691114493	28.082195059913694	28.382086548984383	21.865263699987434
80-81	21.657738532141025	28.05347128671476	28.416029455322413	21.872760725821806
82-83	21.800419616071313	28.028082006659854	28.488116740680436	21.683381636588397
84-85	21.967523580045153	27.9525748523677	28.354499482364208	21.72540208522293
86-87	21.7110200982436	28.063881419678356	28.40198673012656	21.823111751951487
88-89	21.84259288716766	27.98684874316899	28.36440466581954	21.806153703843812
90-91	21.90904680011429	28.040627308063463	28.325476707764796	21.724849184057447
92-93	21.89391836809032	28.026469983007736	28.36589662799665	21.713715020905294
94-95	21.76607318571347	28.144751341389913	28.32807796642508	21.76109750647154
96-97	21.824747868763307	28.276649564132832	28.416615277509788	21.48198728959407
98-99	22.895129251140162	30.136934459270027	28.666912098541935	18.301024191047876
100	21.800901069262125	31.145327723537402	22.62975599408042	24.424015213120054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1261.0
1	1268.5
2	2145.0
3	3385.5
4	4481.0
5	6297.5
6	7923.0
7	9780.5
8	12468.5
9	14223.5
10	14184.5
11	14048.5
12	15559.5
13	19001.0
14	25534.0
15	33143.0
16	38995.0
17	43056.0
18	43530.0
19	40465.0
20	36992.5
21	34987.5
22	36932.0
23	44147.5
24	55221.5
25	70577.5
26	92061.0
27	116122.0
28	139541.0
29	166737.5
30	195049.0
31	221840.5
32	252079.0
33	281806.0
34	311337.0
35	341111.5
36	370310.0
37	396898.0
38	413318.5
39	424404.5
40	439469.0
41	451793.5
42	457565.0
43	463997.5
44	494597.0
45	545507.0
46	541614.0
47	513555.0
48	490962.0
49	454297.5
50	428971.5
51	404412.5
52	378526.5
53	353314.5
54	326935.5
55	297006.5
56	266702.5
57	234579.0
58	203252.5
59	175584.0
60	145761.5
61	115381.0
62	88706.5
63	66805.0
64	48728.0
65	35404.5
66	25489.0
67	18936.5
68	14176.5
69	10167.0
70	7444.0
71	5466.0
72	3750.0
73	2450.0
74	1639.0
75	1029.5
76	629.0
77	344.0
78	202.0
79	133.0
80	88.5
81	60.5
82	42.5
83	31.0
84	24.0
85	19.0
86	13.0
87	12.0
88	8.5
89	4.0
90	2.5
91	1.5
92	1.0
93	1.0
94	1.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0026237909870490384
3	0.0
4	0.0
5	0.0024300882967299817
6	1.1446068064307884E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.150077428903202E-5
44-45	9.052825909767134E-6
46-47	0.0
48-49	0.0
50-51	1.3850025460963473E-5
52-53	0.001016872468033987
54-55	0.0021294683109897936
56-57	0.002400869124019643
58-59	0.0016075078559854516
60-61	0.003978558804193706
62-63	0.0016196981361874779
64-65	0.001621590082007575
66-67	1.607047790874189E-4
68-69	1.2792068759927999E-4
70-71	1.9396624718730289E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	6.177381979186855E-5
80-81	3.008600239401583E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.6173288810785084E-5
92-93	2.446488500688551E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	77.0
20-21	277.0
22-23	564.0
24-25	882.0
26-27	2057.0
28-29	5630.0
30-31	16618.0
32-33	20897.0
34-35	28792.0
36-37	45843.0
38-39	55874.0
40-41	54729.0
42-43	61445.0
44-45	73109.0
46-47	75052.0
48-49	69515.0
50-51	62840.0
52-53	60866.0
54-55	63932.0
56-57	66633.0
58-59	67410.0
60-61	73122.0
62-63	72283.0
64-65	86497.0
66-67	101685.0
68-69	103122.0
70-71	140598.0
72-73	75556.0
74-75	68587.0
76-77	71863.0
78-79	73731.0
80-81	73717.0
82-83	70329.0
84-85	70955.0
86-87	72762.0
88-89	75443.0
90-91	78012.0
92-93	78779.0
94-95	98140.0
96-97	337709.0
98-99	782200.0
100-101	7919480.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.17951626289327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.43573698723841	67.57504105488971
2	8.381386624508616	13.10505503832735
3	2.5279493712351258	5.929015769407419
4	1.0998174971035168	3.439327996040759
5	0.5455544864179234	2.132559292160221
6	0.315136788666832	1.4782345016768732
7	0.19969210867830764	1.092828272059104
8	0.1275705591346066	0.7978723682024291
9	0.08486434656332714	0.59711882060587
>10	0.27640172683648606	3.287501387723997
>50	0.004313451450879513	0.2237410900432759
>100	0.001432124773748053	0.20315295559071778
>500	9.595179273912462E-5	0.06241205724330809
>1k	4.7975599534003064E-5	0.07613939602900949
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.641400322532589E-5	0.0
4	0.0	8.804667741775296E-6	0.0	2.641400322532589E-5	0.0
5	0.0	8.804667741775296E-6	0.0	2.641400322532589E-5	0.0
6	0.0	8.804667741775296E-6	0.0	3.521867096710118E-5	0.0
7	0.0	8.804667741775296E-6	0.0	3.521867096710118E-5	0.0
8	0.0	1.760933548355059E-5	0.0	3.521867096710118E-5	0.0
9	0.0	6.163267419242707E-5	0.0	4.402333870887648E-5	0.0
10-11	0.0	7.043734193420236E-5	0.0	4.8425672579764125E-5	0.0
12-13	0.0	7.043734193420236E-5	0.0	6.163267419242707E-5	0.0
14-15	0.0	1.188630145139665E-4	0.0	7.924200967597765E-5	0.0
16-17	0.0	1.3647234999751708E-4	0.0	7.924200967597765E-5	0.0
18-19	0.0	1.5408168548106766E-4	0.0	7.924200967597765E-5	0.0
20-21	0.0	1.8049568870639356E-4	0.0	7.924200967597765E-5	0.0
22-23	0.0	2.0690969193171946E-4	0.0	7.924200967597765E-5	0.0
24-25	0.0	2.509330306405959E-4	0.0	7.924200967597765E-5	0.0
26-27	0.0	2.553353645114836E-4	0.0	9.24490112886406E-5	0.0
28-29	0.0	2.641400322532589E-4	0.0	2.509330306405959E-4	0.0
30-31	0.0	3.213703725747983E-4	0.0	8.232364338559902E-4	0.0
32-33	0.0	3.2577270644568596E-4	0.0	0.001985452575770329	0.0
34-35	0.0	3.87405380638113E-4	0.0	0.00364953477896586	0.0
36-37	0.0	4.226240516052142E-4	0.0	0.005938748391827437	0.0
38-39	0.0	4.402333870887648E-4	0.0	0.009790790528854128	0.0
40-41	0.0	4.974637274103042E-4	0.0	0.01407426138522781	0.0
42-43	0.0	5.238777306356301E-4	0.0	0.017807440507740538	0.0
44-45	0.0	5.899127386989448E-4	0.0	0.022029278689921793	0.0
46-47	0.0	6.295337435369337E-4	0.0	0.026766189934996902	0.0
48-49	0.0	6.339360774078212E-4	0.0	0.031300593822011176	0.0
50-51	0.0	7.13178087083799E-4	0.0	0.03595386072353942	0.0
52-53	0.0	7.307874225673496E-4	0.0	0.04064674862990565	0.0
54-55	0.0	7.307874225673496E-4	0.0	0.04549812055562384	0.0
56-57	0.0	7.660060935344508E-4	0.0	0.05005453611199256	0.0
58-59	0.0	7.792130951471137E-4	0.0	0.05447007698449287	0.0
60-61	0.0	8.012247645015519E-4	0.0	0.05901768787311981	0.0
62-63	0.0	8.012247645015519E-4	0.0	0.0631911003827213	8.804667741775296E-6
64-65	0.0	9.068807774028555E-4	0.0	0.06760223892135073	8.804667741775296E-6
66-67	0.0	9.244901128864061E-4	0.0	0.07211463113901057	8.804667741775296E-6
68-69	0.0	9.465017822408443E-4	0.0	0.07623081330829051	8.804667741775296E-6
70-71	0.0	9.597087838535071E-4	0.0	0.07989795742273992	8.804667741775296E-6
72-73	0.0	9.729157854661702E-4	0.0	0.08273306043559156	8.804667741775296E-6
74-75	0.0	0.0010169391241750467	0.0	0.08496064137426071	8.804667741775296E-6
76-77	0.0	0.001074169464496586	0.0	0.08717061297744631	8.804667741775296E-6
78-79	0.0	0.0011093881354636874	0.0	0.08866300415967723	8.804667741775296E-6
80-81	0.0	0.0011093881354636874	0.0	0.08916046788708754	8.804667741775296E-6
82-83	0.0	0.001113790469334575	0.0	0.089495045261275	8.804667741775296E-6
84-85	0.0	0.0011358021386890132	0.0	0.0897063572870776	8.804667741775296E-6
86-87	0.0	0.001175423143527002	0.0	0.08977679462901181	8.804667741775296E-6
88	0.0	0.0012062394806232155	0.0	0.08978119696288268	8.804667741775296E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13060	0.0	51.862556	1
CTTATAC	14005	0.0	48.828114	1
GTATCAA	25040	0.0	39.499523	1
TATACAC	19100	0.0	39.25726	3
TTATACA	20280	0.0	36.010616	2
ATCAACG	28425	0.0	33.985256	3
TCAACGC	29005	0.0	33.350574	4
CAACGCA	29070	0.0	33.231197	5
AACGCAG	30220	0.0	32.1238	6
TATCAAC	30690	0.0	31.831575	2
ACGCAGA	33915	0.0	28.52141	7
CGCAGAG	34445	0.0	28.069952	8
TACACAT	27530	0.0	27.346634	5
ACATCTC	26835	0.0	26.662647	8
ACACATC	27180	0.0	26.292383	6
CACATCT	29410	0.0	24.387241	7
CATCTCC	29760	0.0	23.881598	9
GCAGAGT	41020	0.0	23.61302	9
ATACACA	33660	0.0	23.398294	4
GAGTACT	25450	0.0	20.761103	12-13
>>END_MODULE