FastQCFastQC Report
Fri 27 May 2016
SRR2139422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10287951
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128150.048.2866861
GTATCAA230150.038.3236431
CTTATAC96200.035.4811971
ATCAACG261550.033.078513
TCAACGC269400.032.194734
CAACGCA269650.032.1008725
AACGCAG282950.030.8048826
TATCAAC287400.030.3592662
TATACAC132500.029.179543
ACGCAGA313900.027.6299767
CGCAGAG320200.027.0728768
TTATACA147150.025.2194182
ACATCTC154500.023.4319958
ACACATC153550.023.2117616
GCAGAGT382500.022.69729
TACACAT183700.021.7279575
TATGCCG98150.020.39699442-43
CACATCT180700.019.771887
GAGTACT238600.019.75726112-13
CTCGTAT100250.019.61838338-39
Produced by FastQC (version 0.11.5)