##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10287951
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.434300280007164	32.0	18.0	33.0	18.0	33.0
2	28.642120671064628	31.0	27.0	33.0	18.0	33.0
3	30.502253947360362	31.0	29.0	33.0	27.0	33.0
4	31.86751754552486	33.0	31.0	33.0	29.0	33.0
5	32.721871245304335	33.0	33.0	33.0	32.0	34.0
6	36.87312381250649	38.0	37.0	38.0	35.0	38.0
7	36.991566542259	38.0	38.0	38.0	35.0	38.0
8	37.218000941100904	38.0	38.0	38.0	36.0	38.0
9	37.3025902825548	38.0	38.0	38.0	37.0	38.0
10-11	37.30517019375384	38.0	38.0	38.0	36.5	38.0
12-13	37.34599469806962	38.0	38.0	38.0	37.0	38.0
14-15	37.34118489677877	38.0	38.0	38.0	37.0	38.0
16-17	37.35902095567913	38.0	38.0	38.0	37.0	38.0
18-19	37.35999340393437	38.0	38.0	38.0	37.0	38.0
20-21	37.322978176317854	38.0	38.0	38.0	37.0	38.0
22-23	37.340622700541196	38.0	38.0	38.0	37.0	38.0
24-25	37.35533774335196	38.0	38.0	38.0	37.0	38.0
26-27	37.320643829470406	38.0	38.0	38.0	37.0	38.0
28-29	37.297764589058914	38.0	38.0	38.0	37.0	38.0
30-31	37.30062566061369	38.0	38.0	38.0	37.0	38.0
32-33	37.233705994435766	38.0	38.0	38.0	37.0	38.0
34-35	37.23713488058223	38.0	38.0	38.0	37.0	38.0
36-37	37.235788847675565	38.0	38.0	38.0	37.0	38.0
38-39	37.211802530991875	38.0	38.0	38.0	37.0	38.0
40-41	37.223355319982325	38.0	38.0	38.0	37.0	38.0
42-43	37.1953552245881	38.0	38.0	38.0	37.0	38.0
44-45	37.17085352313492	38.0	38.0	38.0	36.0	38.0
46-47	37.144386050946586	38.0	38.0	38.0	36.0	38.0
48-49	37.102255173736644	38.0	38.0	38.0	36.0	38.0
50-51	37.07779967581316	38.0	38.0	38.0	36.0	38.0
52-53	37.049962889519904	38.0	38.0	38.0	36.0	38.0
54-55	37.024938769348545	38.0	38.0	38.0	36.0	38.0
56-57	36.99304771089322	38.0	38.0	38.0	36.0	38.0
58-59	36.9988213148179	38.0	38.0	38.0	36.0	38.0
60-61	37.0223782571682	38.0	38.0	38.0	36.0	38.0
62-63	37.03219765354109	38.0	38.0	38.0	36.0	38.0
64-65	37.03002764444162	38.0	38.0	38.0	36.0	38.0
66-67	37.03332616527915	38.0	38.0	38.0	36.0	38.0
68-69	37.033995662300114	38.0	38.0	38.0	36.0	38.0
70-71	37.0249888710366	38.0	38.0	38.0	36.0	38.0
72-73	37.00473515769339	38.0	38.0	38.0	36.0	38.0
74-75	37.00504822688796	38.0	38.0	38.0	36.0	38.0
76-77	37.00615357656466	38.0	38.0	38.0	36.0	38.0
78-79	36.98419155781062	38.0	38.0	38.0	36.0	38.0
80-81	36.96157410516366	38.0	38.0	38.0	36.0	38.0
82-83	36.967639310394766	38.0	38.0	38.0	36.0	38.0
84-85	36.919535631820025	38.0	38.0	38.0	35.5	38.0
86-87	36.909606040672166	38.0	38.0	38.0	35.5	38.0
88-89	36.91762062164094	38.0	38.0	38.0	35.5	38.0
90-91	36.89777809986222	38.0	38.0	38.0	35.0	38.0
92-93	36.90645646463677	38.0	38.0	38.0	35.0	38.0
94-95	36.8597287596961	38.0	38.0	38.0	35.0	38.0
96-97	36.83775330489269	38.0	38.0	38.0	35.0	38.0
98-99	36.91319022610593	38.0	38.0	38.0	35.0	38.0
100	35.33969443864708	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	15.0
19	54.0
20	111.0
21	332.0
22	870.0
23	2192.0
24	5026.0
25	10284.0
26	17832.0
27	29703.0
28	46190.0
29	66111.0
30	89402.0
31	117823.0
32	153597.0
33	206061.0
34	304849.0
35	552961.0
36	1695101.0
37	6989431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62660747509392	19.161949741012567	13.955315300393636	23.25612748349987
2	13.779719861337203	20.387315287755044	42.7323487845815	23.100616066326253
3	17.8794883451525	26.4711311319426	30.934342513878615	24.715038009026287
4	11.691871394021998	18.60327678465809	40.57531961417779	29.129532207142123
5	12.716513535492016	38.08688322183767	36.183006609644494	13.013596633025825
6	28.48952963474421	39.03257221245728	19.50860721304681	12.969290939751696
7	25.497110697466454	33.17103990590934	23.910069547383102	17.421779849241105
8	23.393317094920064	37.33979681668391	21.907773472093712	17.359112616302312
9	25.07429321932035	17.17119375860169	21.921226102262732	35.83328691981523
10-11	23.4041258555761	27.17211619689868	29.67635149117643	19.74740645634879
12-13	24.479514919929148	24.634084085353827	29.66524626721103	21.221154727505994
14-15	21.65111400705544	26.055159088529873	27.25319648198169	25.040530422433
16-17	21.158931452920022	29.250625318880306	27.91971890223816	21.670724325961505
18-19	20.981092347737658	28.31968192694541	29.77991924728257	20.919306478034354
20-21	21.91911652815519	27.507866010489828	29.52246090517819	21.050556556176794
22-23	21.608651036662323	27.657444929432952	29.35411030633596	21.379793727568767
24-25	21.49491163531679	27.770452857943607	29.542427277204325	21.192208229535282
26-27	21.520177809899682	27.951791673514688	29.336251526556012	21.19177899002962
28-29	21.221410724675422	28.01326204152936	29.419543540166504	21.345783693628714
30-31	21.859079019644252	27.750934212518896	29.350545656413008	21.039441111423844
32-33	21.20034607135894	28.028670306102555	29.386703061025543	21.384280561512966
34-35	21.446127498214334	28.051554129965687	29.474820045139953	21.027498326680025
36-37	21.390030106050794	27.991517465105996	29.346294650832625	21.272157778010577
38-39	21.244989211661245	27.767718137225817	29.59974404007124	21.387548611041698
40-41	21.49045953770475	27.597456444402596	29.57346273299152	21.338621284901137
42-43	21.56530544275497	27.57551499546723	29.44494642573556	21.41423313604224
44-45	21.46955483065685	27.987946124737327	29.18098755815478	21.36151148645104
46-47	21.59817999632756	27.72652567706769	28.866345335710474	21.80894899089428
48-49	21.667813437495326	27.688965636451705	28.93729596830782	21.705924957745147
50-51	21.403216813052172	27.829926824665062	28.974719478155702	21.792136884127064
52-53	21.506502075709832	27.900594877997513	29.001699798750924	21.591203247541728
54-55	21.615053754176802	27.81468086589428	28.84952032790253	21.72074505202639
56-57	21.844548516880682	28.051696680807936	28.485587526135987	21.61816727617539
58-59	21.458461796315447	28.280060653653344	28.943484990979123	21.317992559052087
60-61	21.713442813566118	28.56268459000802	28.4085646264008	21.31530797002506
62-63	21.506918766268186	28.667286895439855	28.37886876510379	21.446925573188167
64-65	21.419881821341676	28.675359502233473	28.428693029267027	21.476065647157828
66-67	21.532055704920086	28.60861865721269	28.337195610299997	21.522130027567226
68-69	21.56332482083266	28.458576361776423	28.455497706292103	21.522601111098815
70-71	21.562344833957987	28.094041651648556	28.569096277010818	21.77451723738264
72-73	21.696847865726106	27.96382618851056	28.72436891314124	21.614957032622094
74-75	21.69072028938769	28.02966268923975	28.601937445170368	21.67767957620219
76-77	21.618868915833954	28.084697236326704	28.491131480881336	21.805302366958003
78-79	21.658989688283476	28.06142859034421	28.48216969012116	21.797412031251156
80-81	21.62113954889303	28.106895722541804	28.45745463539751	21.81451009316766
82-83	21.730045175343214	27.950946719152437	28.688381227851846	21.63062687765251
84-85	21.98082213662081	27.962883087654355	28.394656724785005	21.661638050939832
86-87	21.64399609810959	28.109798650472655	28.487376838798482	21.758828412619277
88-89	21.845347306021022	27.99469560694491	28.423950945735243	21.736006141298827
90-91	21.86473898924898	28.09534373804506	28.41319567039872	21.62672160230724
92-93	21.861154051770118	28.018375925147136	28.437707852188716	21.68276217089403
94-95	21.708392774464	28.18076100460568	28.40190511455054	21.708941106379783
96-97	21.772171157575364	28.273369157589496	28.497217105756235	21.45724257907891
98-99	22.843380160727328	30.17173890847551	28.716856487915816	18.268024442881345
100	21.769436197988764	31.122592259737086	22.637649554265465	24.470321988008685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	881.0
1	963.0
2	1924.5
3	3185.5
4	4412.0
5	6407.0
6	8063.5
7	9631.5
8	11659.0
9	12943.0
10	12925.5
11	12953.5
12	14432.5
13	17712.5
14	24170.0
15	31687.0
16	37421.0
17	41463.0
18	41982.5
19	38934.5
20	35273.5
21	33015.5
22	34757.5
23	41209.5
24	51488.0
25	65957.0
26	85665.5
27	108987.5
28	131827.5
29	157140.0
30	184206.5
31	208642.0
32	236641.5
33	264887.5
34	290875.0
35	318097.0
36	344986.0
37	370597.0
38	386104.0
39	395171.0
40	408444.5
41	418270.0
42	425062.0
43	447457.5
44	465002.5
45	464502.5
46	454432.0
47	440691.5
48	427984.0
49	409630.0
50	390974.5
51	370563.5
52	346629.0
53	322158.0
54	299557.0
55	274381.5
56	246044.0
57	215185.5
58	184752.5
59	158471.5
60	129505.0
61	100612.0
62	77271.5
63	57941.5
64	42132.5
65	30503.5
66	22750.5
67	17285.5
68	12612.5
69	9079.5
70	6751.0
71	4885.0
72	3438.0
73	2393.0
74	1676.0
75	1133.0
76	716.5
77	419.5
78	249.0
79	157.5
80	112.5
81	74.5
82	43.5
83	22.5
84	14.0
85	14.5
86	13.0
87	10.0
88	5.5
89	2.5
90	1.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002527228210943073
3	0.0
4	0.0
5	0.0024980678854321916
6	9.720108503627204E-5
7	9.720108503627204E-6
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.471820721804157E-5
44-45	2.0042354505659083E-5
46-47	0.0
48-49	0.0
50-51	1.5369092601088284E-5
52-53	0.0011241110370068697
54-55	0.002065619114055962
56-57	0.0026077820082377693
58-59	0.0016107511427384494
60-61	0.0037965148629845206
62-63	0.0016183799247821878
64-65	0.0015119149931444608
66-67	2.3335326966189877E-4
68-69	1.643407700088174E-4
70-71	2.0469773557832532E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	5.7320081007031276E-5
80-81	2.1392419890880156E-4
82-83	1.166402136382153E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.0208309858959213E-5
92-93	1.829782082102932E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	116.0
20-21	305.0
22-23	563.0
24-25	850.0
26-27	1934.0
28-29	5328.0
30-31	16248.0
32-33	20237.0
34-35	27809.0
36-37	45699.0
38-39	55878.0
40-41	55035.0
42-43	61509.0
44-45	73519.0
46-47	75786.0
48-49	70919.0
50-51	64350.0
52-53	61885.0
54-55	65603.0
56-57	68579.0
58-59	68526.0
60-61	69379.0
62-63	67416.0
64-65	75838.0
66-67	84559.0
68-69	86527.0
70-71	105199.0
72-73	72754.0
74-75	70330.0
76-77	73635.0
78-79	74818.0
80-81	75245.0
82-83	72117.0
84-85	71624.0
86-87	74317.0
88-89	77506.0
90-91	78913.0
92-93	78702.0
94-95	95933.0
96-97	304364.0
98-99	694667.0
100-101	7043430.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.47554304270177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.69273164229617	68.03259193484408
2	8.046301088137911	12.628756947534095
3	2.5534731285406647	6.011555712215259
4	1.0909603381196162	3.4245481988794566
5	0.5652408477088403	2.2178791236934168
6	0.34121197091151134	1.606607682597085
7	0.2096132859406629	1.1514661510211053
8	0.13487527951839037	0.8467528642593501
9	0.09083909139065016	0.6415782323748198
>10	0.2718263750314033	3.0827755872250675
>50	0.0020466098551413367	0.10063358112078662
>100	7.048478112845841E-4	0.12256404605840841
>500	1.2535370187036617E-4	0.06726662421187891
>1k	5.014103589515774E-5	0.06502331396517592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.720108503627204E-6	0.0	0.0	0.0
3	0.0	1.9440217007254407E-5	0.0	0.0	0.0
4	0.0	2.9160325510881614E-5	0.0	0.0	0.0
5	0.0	7.776086802901763E-5	0.0	9.720108503627204E-6	0.0
6	0.0	7.776086802901763E-5	0.0	9.720108503627204E-6	0.0
7	0.0	7.776086802901763E-5	0.0	9.720108503627204E-6	0.0
8	0.0	7.776086802901763E-5	0.0	1.9440217007254407E-5	0.0
9	0.0	7.776086802901763E-5	0.0	1.9440217007254407E-5	0.0
10-11	0.0	7.776086802901763E-5	0.0	1.9440217007254407E-5	0.0
12-13	0.0	8.262092228083123E-5	0.0	2.9160325510881614E-5	0.0
14-15	0.0	1.4094157330259448E-4	0.0	2.9160325510881614E-5	0.0
16-17	0.0	1.5552173605803526E-4	0.0	2.9160325510881614E-5	0.0
18-19	0.0	1.6524184456166247E-4	0.0	2.9160325510881614E-5	0.0
20-21	0.0	1.7982200731710328E-4	0.0	4.860054251813602E-5	0.0
22-23	0.0	1.992622243243577E-4	0.0	5.3460596769949625E-5	0.0
24-25	0.0	2.284225498352393E-4	0.0	7.290081377720403E-5	0.0
26-27	0.0	2.6244292959793454E-4	0.0	1.1664130204352646E-4	0.0
28-29	0.0	2.8188314660518895E-4	0.0	2.575828753461209E-4	0.0
30-31	0.0	3.5964401463420655E-4	0.0	0.00102547144713267	0.0
32-33	0.0	4.27684774159597E-4	0.0	0.0029014523883327207	0.0
34-35	0.0	5.394660219513099E-4	0.0	0.0051127770729079095	0.0
36-37	0.0	6.852676495057179E-4	0.0	0.008650896568228213	0.0
38-39	0.0	6.901277037575315E-4	0.0	0.013992096190971361	0.0
40-41	0.0	7.678885717865491E-4	0.0	0.019950522703694837	0.0
42-43	0.0	7.970488972974307E-4	0.0	0.025957549758936452	0.0
44-45	0.0	8.359293313119396E-4	0.0	0.03219785941826511	0.0
46-47	0.0	8.602296025710075E-4	0.0	0.03943448019921557	0.0
48-49	0.0	8.699497110746348E-4	0.0	0.046836342824727685	0.0
50-51	0.0	0.0010351915556362974	0.0	0.05401464295465637	0.0
52-53	0.0	0.0011080923694135013	0.0	0.06079441863593635	0.0
54-55	0.0	0.0011178124779171285	0.0	0.06759849458847539	0.0
56-57	0.0	0.0011566929119316374	0.0	0.07212320509691386	0.0
58-59	0.0	0.0011809931831907052	0.0	0.07634173218748806	0.0
60-61	0.0	0.001210153508701587	0.0	0.0806039997663286	0.0
62-63	0.0	0.0012636141054715366	0.0	0.08489542767068001	0.0
64-65	0.0	0.0013219347564932997	0.0	0.0890799343814915	0.0
66-67	0.0	0.0013267948107451135	0.0	0.09340538266560562	0.0
68-69	0.0	0.0013365149192487405	0.0	0.09776485132948243	0.0
70-71	0.0	0.0013510950820041813	0.0	0.10177925614148045	0.0
72-73	0.0	0.0013608151905078086	0.0	0.10485567048287847	0.0
74-75	0.0	0.0013802554075150629	0.0	0.1074703796703542	0.0
76-77	0.0	0.001419135841529572	0.0	0.1099052668505128	0.0
78-79	0.0	0.001428855950033199	0.0	0.11130982252928692	0.0
80-81	0.0	0.001428855950033199	0.0	0.11184442849698642	0.0
82-83	0.0	0.001428855950033199	0.0	0.11228183337964964	0.0
84-85	0.0	0.0014531562212922672	0.0	0.11246165538696676	0.0
86-87	0.0	0.0014871766010549623	0.0	0.11256857658050665	0.0
88	0.0	0.0015454972520767254	0.0	0.11257829668901027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12815	0.0	48.286686	1
GTATCAA	23015	0.0	38.323643	1
CTTATAC	9620	0.0	35.481197	1
ATCAACG	26155	0.0	33.07851	3
TCAACGC	26940	0.0	32.19473	4
CAACGCA	26965	0.0	32.100872	5
AACGCAG	28295	0.0	30.804882	6
TATCAAC	28740	0.0	30.359266	2
TATACAC	13250	0.0	29.17954	3
ACGCAGA	31390	0.0	27.629976	7
CGCAGAG	32020	0.0	27.072876	8
TTATACA	14715	0.0	25.219418	2
ACATCTC	15450	0.0	23.431995	8
ACACATC	15355	0.0	23.211761	6
GCAGAGT	38250	0.0	22.6972	9
TACACAT	18370	0.0	21.727957	5
TATGCCG	9815	0.0	20.396994	42-43
CACATCT	18070	0.0	19.77188	7
GAGTACT	23860	0.0	19.757261	12-13
CTCGTAT	10025	0.0	19.618383	38-39
>>END_MODULE